Growth regulation最新文献_第3页

Streptozotocin induction of diabetes in rats leads to increased insulin-like growth factor-II/mannose-6-phosphate receptor mRNA expression in kidney but not in lung or heart. 链脲佐菌素诱导大鼠糖尿病导致肾脏中胰岛素样生长因子- ii /甘露糖-6-磷酸受体mRNA表达增加，但在肺和心脏中没有。

Growth regulation Pub Date : 1996-06-01

W Kiess, A Hoeflich, Y Yang, H Groenbaek, A Flyvbjerg

{"title":"Streptozotocin induction of diabetes in rats leads to increased insulin-like growth factor-II/mannose-6-phosphate receptor mRNA expression in kidney but not in lung or heart.","authors":"W Kiess, A Hoeflich, Y Yang, H Groenbaek, A Flyvbjerg","doi":"","DOIUrl":"","url":null,"abstract":"Insulin and glucose are thought to act as important modulators of the expression of the IGFs, their binding proteins and their receptors. It has been postulated that changes of the IGF system after diabetes onset contribute to the development of diabetes late complications. We have measured the expression of IGF-II/M6P receptor mRNA in rat kidney, lung and heart after streptozotocin induction of diabetes. Adult rats were injected with streptozotocin, and, after the onset of diabetes, were treated with either insulin or vehicle. The rats were sacrificed on days 1, 2, 3, 4 and 9. Kidneys, lungs and hearts were removed aseptically and RNA was extracted from the tissues. In solution hybridization/RNAse protection experiments, specific IGF-II/M6P receptor and beta-actin transcripts were detected in the RNA samples from all tissues examined. To gain additional evidence for the expression of IGF-II/M6P receptor RNA in the tissues examined, Northern blotting experiments were performed: a major 9 kb RNA species was detected on the blots. Interestingly, streptozotocin-induced onset of diabetes led to a significant increase in the expression of IGF-II/M6P receptor mRNA in the kidney but not in lung and heart whereas no change in actin mRNA expression was measured. Insulin treatment did not prevent the increase of IGF-II/M6P receptor mRNA expression during short-term treatment (1-9 days). Alterations of the IGF system during diabetes onset might be of relevance for the development of early renal changes during the course of the disease.","PeriodicalId":77148,"journal":{"name":"Growth regulation","volume":"6 2","pages":"66-72"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19753179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical localization of insulin-like growth factor binding protein-1, -3 and -4 in human fetal tissues and their analysis in media from fetal tissue explants. 人胎组织中胰岛素样生长因子结合蛋白-1、-3和-4的免疫组化定位及其在胎组织外植体培养基中的分析。

Growth regulation Pub Date : 1996-06-01

T Braulke, W Götz, M Claussen

{"title":"Immunohistochemical localization of insulin-like growth factor binding protein-1, -3 and -4 in human fetal tissues and their analysis in media from fetal tissue explants.","authors":"T Braulke, W Götz, M Claussen","doi":"","DOIUrl":"","url":null,"abstract":"The actions of insulin-like growth factor (IGF) II that are important in the regulation of fetal growth and development, are modulated by IGF binding proteins (IGFBPs). We have determined the cellular distribution of IGF-II and IGFBP-1, -3 and -4 in 12-week gestation human fetal tissues using immunocytochemistry. IGF-II immunostaining was found in all organs examined, with strongest immunoreactivity in spinal ganglia, tubular cells of the mesonephros and peri- and epidermal layers of the skin. The immunoreactivity distribution of all IGFBPs was similar to that of IGF-II except lung, hepatic parenchyma, fibrocytes of connective tissue and cells of the growth plate in the cartilage. When conditioned media from skin, liver, lung and kidney explants were analyzed, phosphorylated IGFBP-1 was only detected in liver samples whereas IGFBP-3 was found in all media. Weak immunoreactivity of IGFBP-4 was seen in media from lung tissue. To determine whether proteolytic degradation of IGFBPs were responsible for the different IGFBP levels, cell-free conditioned media were incubated with recombinant human IGFBPs. At neutral pH only proteolysis of IGFBP-4 was observed in media from skin and lung tissue. Upon acidification of the medium samples, IGFBP-1 fragments were formed in skin-derived medium and IGFBP-3 was cleaved by medium from lung and kidney tissue. Acid-activated proteolytic activity against IGFBP-4 was found in the media from lung and liver. These findings suggest that IGFBP proteases may be important in locally defining the concentrations of IGFBPs and contribute to tissue-specific growth response to IGFs.","PeriodicalId":77148,"journal":{"name":"Growth regulation","volume":"6 2","pages":"55-65"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19753178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discordant Regulation of insulin-like growth factor-II and insulin-like growth factor binding protein-2 gene expression in a rat beta-cell line. 大鼠β细胞系中胰岛素样生长因子- ii和胰岛素样生长因子结合蛋白-2基因表达的不一致调控。

Growth regulation Pub Date : 1996-06-01

W De, P Czernichow, M Asfari

引用次数: 0

Postnatal age and food intake alter insulin-like growth factor-II/mannose-6-phosphate receptors in ovine skeletal muscles. 出生后年龄和食物摄入改变绵羊骨骼肌中胰岛素样生长因子- ii /甘露糖-6-磷酸受体。

Growth regulation Pub Date : 1996-06-01

J M Oldham, J A Martyn, J R Napier, J J Bass

引用次数: 0

Studies on stimulation of DNA synthesis with epidermal growth factor and insulin-like growth factor-I in cultured human keratinocytes. 表皮生长因子和胰岛素样生长因子- 1刺激人角质形成细胞DNA合成的研究。

Growth regulation Pub Date : 1996-06-01

H J Ristow

{"title":"Studies on stimulation of DNA synthesis with epidermal growth factor and insulin-like growth factor-I in cultured human keratinocytes.","authors":"H J Ristow","doi":"","DOIUrl":"","url":null,"abstract":"Epidermal growth factor (EGF) and insulin are the most widely used growth factors (GFs) in human keratinocyte cultures. Insulin is supposed to exert its growth-promoting activity in this system through the insulin-like growth factor-I (IGF-I) receptor. In order to obtain more information about the contribution of EGF and IGF-I to the proliferation of keratinocytes, the effect of each factor on DNA synthesis was studied with 3H thymidine incorporation in an otherwise GF-free system. Both factors are able to initiate DNA synthesis, DNA synthesis peaks 18-20 h after the addition of each factor, and neither factor influences significantly the binding of the other to the respective receptor. IGF-I is the more potent growth factor, and IGF-I-stimulated cells enter the S-phase regularly approximately 3 h earlier than EGF-stimulated keratinocytes (7-9 h vs 10-12 h). However, IGF-I-stimulated DNA synthesis can be completely turned off by the addition of the monoclonal antibody (mAb) to the EGF receptor LA1. This inhibition cannot be reversed by higher IGF-I concentrations, but only by the addition of EGF to the culture medium. These results may suggest the presence of two keratinocyte populations, one responding to EGF and one to IGF-I, with an additional signal from the EGF receptor, or they may be explained on the basis of only one cell population for which EGF acts as a \"competence' factor and IGF-I as a \"progression' factor.","PeriodicalId":77148,"journal":{"name":"Growth regulation","volume":"6 2","pages":"96-109"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19753093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ontogeny of growth hormone protein and mRNA in the gilthead sea bream Sparus aurata. 金颡鱼生长激素蛋白和mRNA的个体发育。

Growth regulation Pub Date : 1996-03-01

B Funkenstein, I Cohen

{"title":"Ontogeny of growth hormone protein and mRNA in the gilthead sea bream Sparus aurata.","authors":"B Funkenstein, I Cohen","doi":"","DOIUrl":"","url":null,"abstract":"The ontogeny of growth hormone (GH) protein and mRNA was studied in the gilthead sea bream Sparus aurata by Northern blot, reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analyses. Total RNA was prepared from a pool of larvae collected on different days after hatching. When hybridized to Sparus aurata GH cDNA, GH specific mRNA was first seen on day 3 post-hatching. Levels increased on subsequent days and reached the highest levels on day 9; thereafter a decrease was noted to similar levels between days 10 and 16. RT-PCR amplification of larval RNA showed an amplified fragment on day 3, which hybridized to Sparus aurata GH cDNA but not on days 1 and 2 after hatching. Soluble proteins were electrophoresed and immunoreacted with antibodies raised against purified Sparus GH. A faint band was detected on day 2 after hatching, which had an electrophoretic mobility very similar to recombinant tilapia GH or Sparus pituitary extract. The immunoreactive material increased on days 3, 4 and 6. Specificity of the immunoreaction was demonstrated by the ability of recombinant tilapia GH to complete with GH bound to the membrane and inability of normal rabbit serum (NRS) to detect GH on the membrane. Our results suggest that both GH mRNA and protein are expressed in Sparus aurata shortly after hatching.","PeriodicalId":77148,"journal":{"name":"Growth regulation","volume":"6 1","pages":"16-21"},"PeriodicalIF":0.0,"publicationDate":"1996-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19691911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of epidermal growth factor on circulating levels of free and total IGF-I and IGF-binding proteins in adult rats. 表皮生长因子对成年大鼠游离和总IGF-I及igf结合蛋白循环水平的影响。

Growth regulation Pub Date : 1996-03-01

J Frystyk, L Vinter-Jensen, C Skjaerbaek, A Flyvbjerg

{"title":"The effect of epidermal growth factor on circulating levels of free and total IGF-I and IGF-binding proteins in adult rats.","authors":"J Frystyk, L Vinter-Jensen, C Skjaerbaek, A Flyvbjerg","doi":"","DOIUrl":"","url":null,"abstract":"In neonatal rats, systemic administration of epidermal growth factor (EGF) results in reduced body weight gain and decreased levels of circulating IGF-I, which suggests that it be involved in the EGF-induced growth retardation. We investigated the effect of 4 weeks of EGF administration on circulating free and total IGF-I and IGF-binding proteins (IGFBPs) in adult rats treated with saline (controls), 30 (low dose group) and 150 (high dose group) microgram/kg/day EGF. Serum IGF-I was determined in ultrafiltrates (free) and acid-ethanol extracts (total), and serum IGFBPs using Western ligand blotting, which yielded four distinct molecular bands. The IGFBPs were tentatively identified as IGFBP-3 (a double band at 42 and 38 kDa), IGFBP-1 and/or IGFBP-2 (a single band at 30 kDa) and IGFBP-4 (a single band at 24 kDa). EGF administration did not change the body weight, tibia length, or liver, heart and lung weight. In contrast, serum total IGF-I and IGFBP-3 decreased dose-dependently: total IGF-I averaged 1470 +/- 100 micrograms/l (controls; mean +/- SEM), 1030 +/- 60 micrograms/l (low dose group; P < 0.005) and 760 +/- 40 micrograms/l (high dose group; P < 0.005), whereas differences between IGFBP-3 levels reached significance (P < 0.05) between controls and high dose rats, only. When compared to controls, levels of IGFBP-1 and/or IGFBP-2 were increased in the low dose group (P < 0.05), but unchanged in the high dose group. IGFBP-4 was unaffected by EGF. Free IGF-I averaged 74 +/- 6 micrograms/l in controls, and was reduced to 35 +/- 6 micrograms/l (low dose group; P < 0.005) and 57 +/- 5 micrograms/l (high dose group; P < 0.05). Free IGF-I was inversely correlated (r = -0.49, P < 0.05) with IGFBP-1 and/or IGFBP-2. We conclude that in adult rats prolonged EGF administration has a marked depressing effect on circulating total and free IGF-I. Nevertheless, we did not observe any somatic growth retardation.","PeriodicalId":77148,"journal":{"name":"Growth regulation","volume":"6 1","pages":"48-54"},"PeriodicalIF":0.0,"publicationDate":"1996-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19691915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of insulin-like growth factors and IGF-binding proteins in bone tumours. 骨肿瘤中胰岛素样生长因子和igf结合蛋白的调控。

Growth regulation Pub Date : 1996-03-01

W Zumkeller, O Groth, J Commentz

引用次数: 0

Regulation of endothelial IGFBP-3 synthesis and secretion by IGF-I and TGF-beta. igf - 1和tgf - β对内皮细胞IGFBP-3合成和分泌的调节。

Growth regulation Pub Date : 1996-03-01

N E Erondu, B L Dake, D R Moser, M Lin, M Boes, R S Bar

引用次数: 0

Insulin-like growth factor binding protein-3: factors affecting binary and ternary complex formation. 胰岛素样生长因子结合蛋白-3:影响二元和三元络合物形成的因素。

Growth regulation Pub Date : 1996-03-01

S R Holman, R C Baxter

{"title":"Insulin-like growth factor binding protein-3: factors affecting binary and ternary complex formation.","authors":"S R Holman, R C Baxter","doi":"","DOIUrl":"","url":null,"abstract":"IGF binding protein-3 (IGFBP-3), the major serum carrier of IGF-I and IGF-II in adults, circulates predominantly in a ternary complex with IGF and the acid-labile subunit (ALS). The affinities of IGF and ALS binding have not previously been determined under conditions of temperature, ionic strength and pH which approximate those in the circulation. IGF-I and -II binding was optimal at pH 4.0-5.5, and relatively poor at pH 7.4. The addition of 0.1 mol/l NaCl to 50 mmol/l phosphate buffer increased the affinity of IGF-II for IGFBP-3 by 2-fold, and of IGF-I by 4-fold. ALS binding peaked at pH 5.5-6.0, and was markedly reduced at pH 7.4, where only approximately 0.4 mol ALS was bound per mol IGFBP-3, with greatly reduced affinity. ALS affinity was further reduced at 37 degrees C compared to 22 degrees C, and in the presence of 0.1 mol/l NaCl compared to its absence. Under 'physiological' conditions, the affinities of ALS binding to IGF-I-IGFBP-3 and IGF-II-IGFBP-3 complexes (2.5 x 10(8) l/mol and 5.8 x 10(7) l/mol, respectively) are 300-fold and 2000-fold lower than the constants for the formation of the corresponding binary complexes. Thus, ALS binding to IGFBP-3 complexes is much weaker than previously recognised, emphasising the importance of ALS dissociation as a controlling factor in the regulation of IGF bioavailability.","PeriodicalId":77148,"journal":{"name":"Growth regulation","volume":"6 1","pages":"42-7"},"PeriodicalIF":0.0,"publicationDate":"1996-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19691914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0