Biotechnology (Reading, Mass.)最新文献_第10页

Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda. 1989. 噬菌体λ免疫球蛋白库的大型组合文库的生成。1989.

Biotechnology (Reading, Mass.) Pub Date : 1992-01-01

W D Huse, L Sastry, S A Iverson, A S Kang, M Alting-Mees, D R Burton, S J Benkovic, R A Lerner

引用次数: 0

Cloning immunoglobulin variable domains for expression by the polymerase chain reaction. 1989. 利用聚合酶链反应克隆免疫球蛋白可变结构域。1989.

Biotechnology (Reading, Mass.) Pub Date : 1992-01-01

R Orlandi, D H Güssow, P T Jones, G Winter

引用次数: 0

Construction of coliphage lambda charon vectors with BamH1 cloning sites. 1980. 带BamH1克隆位点的噬菌体λ载体的构建。1980.

Biotechnology (Reading, Mass.) Pub Date : 1992-01-01

D L Rimm, D Horness, J Kucera, F R Blattner

引用次数: 0

A technique for expressing eukaryotic genes in bacteria. 1980. 一种在细菌中表达真核基因的技术。1980.

Biotechnology (Reading, Mass.) Pub Date : 1992-01-01

L Guarante, T M Roberts, M Ptashne

引用次数: 0

Molecular cloning of a cDNA encoding human antihaemophilic factor. 1984. 编码人抗血友病因子cDNA的分子克隆。1984.

Biotechnology (Reading, Mass.) Pub Date : 1992-01-01

J J Toole, J L Knopf, J M Wozney, L A Sultzman, J L Buecker, D D Pittman, R J Kaufman, E Brown, C Showemaker, E C Orr

引用次数: 0

A bacterial clone synthesizing proinsulin. 1978. 合成胰岛素原的细菌克隆体。1978.

Biotechnology (Reading, Mass.) Pub Date : 1992-01-01

L Villa-Komaroff, A Efstratiadis, S Broome, P Lomedico, R Tizard, S P Naber, W L Chick, W Gilbert

引用次数: 0

Sexual and parasexual genetics of Aspergillus species. 曲霉种的性遗传学和拟性遗传学。

Biotechnology (Reading, Mass.) Pub Date : 1992-01-01

A J Clutterbuck

引用次数: 0

Current taxonomic schemes of the genus Aspergillus and its teleomorphs. 曲霉属及其远形植物的最新分类方案。

Biotechnology (Reading, Mass.) Pub Date : 1992-01-01

R A Samson

引用次数: 0

Protein synthesis in Aspergillus nidulans. 中性曲霉的蛋白质合成。

Biotechnology (Reading, Mass.) Pub Date : 1992-01-01

S D Martinelli, S Perera, A Sheikh

{"title":"Protein synthesis in Aspergillus nidulans.","authors":"S D Martinelli, S Perera, A Sheikh","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In this review of protein synthesis, we have described a system for translation of mRNA using extracts of A. nidulans. This system is useful for characterizing mutants suspected to have defects in protein synthesis and for assessing the toxicity of various antibiotics and their effects on misreading the genetic code. The well developed genetical system of A. nidulans has enabled us to map at least 27 new genes whose mutation disturbs the level of translational accuracy. These mutants could be used to identify new components of translation or new roles for established components. The abundant fidelity mutations themselves could be used to elucidate the mechanism for maintaining the accuracy of protein synthesis. The large number of mutations in the control of fidelity indicate that mutations in other parts of the translation system could be easily obtained. This would be particularly important for initiation where many factors are thought to be needed and yet their exact roles are unknown. A. nidulans appears to have normal eukaryotic ribosomes and translation factors that can be used to study the mechanism of protein synthesis, its regulation, and the maintenance of its high fidelity. If highly purified factors were used, requirements for hitherto undiscovered factors could be seen. Since A. nidulans has typical eukaryotic responses to inhibitors of translation, it could be used to study new inhibitors, their mode of action, and their potency. Among the fungi, A. nidulans could be a worthy competitor to S. cerevisiae in the field of protein synthesis, particularly because so many translation genes have been identified. The system awaits further exploitation.</p>","PeriodicalId":77041,"journal":{"name":"Biotechnology (Reading, Mass.)","volume":"23 ","pages":"69-87"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12673657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Haemophilus b conjugate vaccines. b型嗜血杆菌结合疫苗。

Biotechnology (Reading, Mass.) Pub Date : 1992-01-01 DOI: 10.1016/b978-0-7506-9265-6.50007-5

P P Vella, R W Ellis

引用次数: 13