{"title":"Current molecular methods for plant genome identification.","authors":"J C Fowler, J M Kijas, M R Thomas","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>At least four molecular methods have been used for plant species and plant varietal identification. These include protein isoenzymes, restriction fragment length polymorphisms and more recently two alternate PCR-based procedures. One approach is based broadly on methods which amplify plant DNAs using short oligonucleotide primers to produce complex multibanded 'fingerprint' patterns. The method can be used on any plant, is relatively cheap and easy to set up, but has a number of technical limitations. A second approach is to isolate short tandem repeats (STRs) from a test plant, each example of which is locus-specific and may show length variation between individuals. Results from several STR loci will provide a genetic profile of plant variety. However, in comparison with random amplification methods STRs are costly to establish in both time and resources.</p>","PeriodicalId":77018,"journal":{"name":"Australasian biotechnology","volume":"4 3","pages":"153-7"},"PeriodicalIF":0.0,"publicationDate":"1994-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18765453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"DNA fingerprinting for wildlife management.","authors":"S M Degnan","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77018,"journal":{"name":"Australasian biotechnology","volume":"4 3","pages":"163-6"},"PeriodicalIF":0.0,"publicationDate":"1994-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18766555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Opinion--genome testing and quality assurance.","authors":"J C Fowler","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Recent discoveries and innovations in DNA technology have provided scientists with the ability to rapidly identify complex organisms, or samples therefrom, by detailed study of their genes and genome. In various disciplines, most notably in human forensic identifications, parentage tests and disease diagnosis, these activities have moved to commercial practice. However the rigour of quality assurance in these and other areas has not, in general, kept pace with technological innovation in molecular diagnostics. Insufficient resources are given to objective and ongoing evaluation of the quality of molecular diagnostic services, yet sustained quality is the key to long term credibility of the industry. The rapidly expanding commercial prospects for this technology must be accompanied by a planned commitment of resources to cost efficient quality assurance programs which include both declared and undeclared trials.</p>","PeriodicalId":77018,"journal":{"name":"Australasian biotechnology","volume":"4 3","pages":"169-71"},"PeriodicalIF":0.0,"publicationDate":"1994-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18768703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The use of pulsed field gel electrophoresis for microbial strain identification.","authors":"A J Hillier, B E Davidson","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77018,"journal":{"name":"Australasian biotechnology","volume":"4 3","pages":"167-8"},"PeriodicalIF":0.0,"publicationDate":"1994-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18766532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Species of origin determination from prehistoric blood residues using ancient genomic DNA.","authors":"T H Loy, K I Matthaei","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77018,"journal":{"name":"Australasian biotechnology","volume":"4 3","pages":"161-2"},"PeriodicalIF":0.0,"publicationDate":"1994-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18766556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Quality control in the food industries with DNA technologies.","authors":"P R Carnegie","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An important part of quality control in the food industry are tests to determine the origin of materials used in processed products. Because of the stability of DNA, tests which make use of DNA in the product can be used to authenticate the species used in meat pies, tinned fish and even the variety of oats used in porridge. Tests based on hybridization, sequencing of oligonucleotides and amplification of DNA are likely to find increasing application with the only inhibition being the unpredictable nature of the market which makes commercial development difficult.</p>","PeriodicalId":77018,"journal":{"name":"Australasian biotechnology","volume":"4 3","pages":"146-9"},"PeriodicalIF":0.0,"publicationDate":"1994-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18766553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The application of DNA fingerprinting to parentage verification in cattle.","authors":"S S Moore, D Vankan","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77018,"journal":{"name":"Australasian biotechnology","volume":"4 2","pages":"107-8"},"PeriodicalIF":0.0,"publicationDate":"1994-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18765387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Applications of DNA fingerprinting for pedigree verification.","authors":"M E Matthews, H McPartlan","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77018,"journal":{"name":"Australasian biotechnology","volume":"4 2","pages":"103-6"},"PeriodicalIF":0.0,"publicationDate":"1994-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18765389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"DNA typing: analysis of restriction fragment length polymorphisms.","authors":"P A McNicholas","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Genetic Technologies Corporation Pty. Ltd. was the first Australian commercial laboratory to use DNA typing for the purpose of determining human identity. Genetic Technologies holds the patient for the use of this technique in Australia (Australian Patent Number 577277) for individual identity in any species. In the five years since its beginning Genetic Technologies has conducted over 1200 paternity and 70 criminal cases.</p>","PeriodicalId":77018,"journal":{"name":"Australasian biotechnology","volume":"4 2","pages":"97-8"},"PeriodicalIF":0.0,"publicationDate":"1994-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18765449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"DNA profiling in forensic science and parentage testing.","authors":"A van Daal","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77018,"journal":{"name":"Australasian biotechnology","volume":"4 2","pages":"92-6"},"PeriodicalIF":0.0,"publicationDate":"1994-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18765448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}