Archives internationales de physiologie, de biochimie et de biophysique最新文献_第2页

Body temperature of the armadillo Chaetophractus villosus (mammalia, dasypodidae). 绒毛毛犰狳(哺乳动物，穿山甲科)的体温。

Archives internationales de physiologie, de biochimie et de biophysique Pub Date : 1994-09-01 DOI: 10.3109/13813459409003937

E B Casanave, J M Affanni

引用次数: 10

Kinetics and inhibition by some aminoacids of lactating rat mammary gland arginase. 泌乳大鼠乳腺精氨酸酶的动力学及几种氨基酸的抑制作用。

Archives internationales de physiologie, de biochimie et de biophysique Pub Date : 1994-09-01 DOI: 10.3109/13813459409003940

J M Fuentes, M L Campo, G Soler

引用次数: 20

In vitro and in vivo effects of HgCl2 on synaptosomal ATP diphosphohydrolase (EC 3.6.1.5) from cerebral cortex of developing rats. HgCl2对发育大鼠大脑皮层突触体ATP二磷酸水解酶(EC 3.6.1.5)的体内外影响。

Archives internationales de physiologie, de biochimie et de biophysique Pub Date : 1994-09-01 DOI: 10.3109/13813459409003939

E M Oliveira, J B Rocha, J J Sarkis

{"title":"In vitro and in vivo effects of HgCl2 on synaptosomal ATP diphosphohydrolase (EC 3.6.1.5) from cerebral cortex of developing rats.","authors":"E M Oliveira, J B Rocha, J J Sarkis","doi":"10.3109/13813459409003939","DOIUrl":"https://doi.org/10.3109/13813459409003939","url":null,"abstract":"The aim of the present investigation was to evaluate the in vitro (10-500 microM) and in vivo (1-21 subcutaneous injections of 2.5 mg/kg each) effects of HgCl2 on the ATP diphosphohydrolase activity (EC 3.6.1.5; apyrase) of synaptosomes from cerebral cortex of rats at different ages (5, 11, 18 and 25 days of life). The in vitro results showed that HgCl2 (from 10 to 500 microM) inhibited the hydrolysis of both substrates by the synaptosomal enzyme at all ages studied. In contrast, HgCl2 injected in vivo did no affected the normal ontogeny of ATP and ADP hydrolysis. The hydrolysis of both nucleotides increased at the same rate as a function of age in control and HgCl2-treated rats (the specific activity of enzyme increased about 5-fold from the first week of postnatal life of weaning). The results of the present study demonstrated that in vitro HgCl2 inhibited the enzyme, but was ineffective when tested in vivo. Probably the absence of an in vivo effect is due to the low permeability of blood-brain barrier to inorganic forms of mercury.","PeriodicalId":77008,"journal":{"name":"Archives internationales de physiologie, de biochimie et de biophysique","volume":"102 5","pages":"251-4"},"PeriodicalIF":0.0,"publicationDate":"1994-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813459409003939","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18847325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Oscillations in rat liver cytosolic enzyme activities of the urea cycle. 尿素循环大鼠肝细胞质酶活性的波动。

Archives internationales de physiologie, de biochimie et de biophysique Pub Date : 1994-09-01 DOI: 10.3109/13813459409003936

J M Fuentes, M R Pascual, G Salido, G Soler, J A Madrid

{"title":"Oscillations in rat liver cytosolic enzyme activities of the urea cycle.","authors":"J M Fuentes, M R Pascual, G Salido, G Soler, J A Madrid","doi":"10.3109/13813459409003936","DOIUrl":"https://doi.org/10.3109/13813459409003936","url":null,"abstract":"Diurnal rhythms were studied in three rat liver enzymes of the urea cycle: arginase, arginosuccinate synthetase and arginosuccinase. In animals synchronized to a 12:12 h light-dark cycle these enzymes were determined at 8 different time points under three different feeding schedules: 24 h of fasting, ad libitum feeding and restricted feeding. Under the three experimental conditions maxima of enzyme activities occurred during the dark period. In all cases the maximum activity of arginosuccinase preceded the one of arginase and these in turn the one of arginosuccinate synthetase. On the other hand, the hepatic protein level was maximal during the light period and decreased to its lowest level during the dark period. The restriction of food between 08.00 h and 14.00 h induced an important phase shift of hepatic protein rhythm and arginosuccinase activity. Our results suggest that the diurnal rhythms of cytosolic enzymes of the urea cycle are not only dependent on the light-dark cycle, but also on the synchronizing and masking effect of food intake.","PeriodicalId":77008,"journal":{"name":"Archives internationales de physiologie, de biochimie et de biophysique","volume":"102 5","pages":"237-41"},"PeriodicalIF":0.0,"publicationDate":"1994-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813459409003936","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18848771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Characterization of extracellular pH drop due to the activation of the secretory process by acetylcholine in the bovine adrenal medulla. 乙酰胆碱激活牛肾上腺髓质的分泌过程，导致细胞外pH值下降。

Archives internationales de physiologie, de biochimie et de biophysique Pub Date : 1994-09-01 DOI: 10.3109/13813459409003938

P N Viglione, C Gomez, J E Pinto

{"title":"Characterization of extracellular pH drop due to the activation of the secretory process by acetylcholine in the bovine adrenal medulla.","authors":"P N Viglione, C Gomez, J E Pinto","doi":"10.3109/13813459409003938","DOIUrl":"https://doi.org/10.3109/13813459409003938","url":null,"abstract":"A progressive and reversible decrease of external pH accompanied the catecholamine release elicited by acetylcholine in decorticated bovine adrenal glands perfused with buffer-free Locke solution adjusted to an initial pH of 7.4. Both the secretory response as well as the extracellular acid shift promoted by the cholinergic agonist were antagonized by hexamethonium plus atropine, Mg2+ and verapamil. Experiments performed to assess the effects of the reduction of external pH on acetylcholine-induced release of catecholamines revealed that increasing the extracellular concentration of H+ significantly and reversibly reduced this secretory response. These findings are consistent with the idea that adrenomedullary activation of secretion by acetylcholine could be associated with a transient acidification of the extracellular fluid. This release of protons, arising mainly from the chromaffin granules, may be involved in a local automodulatory mechanism of the regulated secretory process.","PeriodicalId":77008,"journal":{"name":"Archives internationales de physiologie, de biochimie et de biophysique","volume":"102 5","pages":"247-50"},"PeriodicalIF":0.0,"publicationDate":"1994-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813459409003938","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18847323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Age-dependent reduction of the response of rat cardiac muscle to the phosphodiesterase inhibitor milrinone. 大鼠心肌对磷酸二酯酶抑制剂米立酮反应的年龄依赖性降低。

Archives internationales de physiologie, de biochimie et de biophysique Pub Date : 1994-09-01 DOI: 10.3109/13813459409003942

M Canepari, B Polla, M R Gualea, C Zanardi, C Reggiani

{"title":"Age-dependent reduction of the response of rat cardiac muscle to the phosphodiesterase inhibitor milrinone.","authors":"M Canepari, B Polla, M R Gualea, C Zanardi, C Reggiani","doi":"10.3109/13813459409003942","DOIUrl":"https://doi.org/10.3109/13813459409003942","url":null,"abstract":"This study aimed to investigate whether milrinone effect on cardiac muscle contractility undergoes to age-related changes. Experiments were carried out on papillary muscles isolated from right ventricle of Brown Norway rats belonging to two different age groups: 2 month old and 18 month old. The effect of milrinone (10-100 microM) on rat cardiac muscle in vitro preparations was characterized by a reduction of peak developed tension and of contraction duration. Furthermore, the recovery of contractility after a contractile cycle, i.e. the mechanical restitution was faster in the presence of milrinone than in control conditions. All these effects were reduced in preparations from 18 month old rats compared to preparations from 2 month old rats. The decrease of milrinone effect on the mechanical restitution was particularly pronounced. The reduction of the milrinone effects is likely connected with the reduction of the maximal effect of adrenergic stimulation, although the molecular basis of this link is not yet clearly understood.","PeriodicalId":77008,"journal":{"name":"Archives internationales de physiologie, de biochimie et de biophysique","volume":"102 5","pages":"265-9"},"PeriodicalIF":0.0,"publicationDate":"1994-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813459409003942","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18847327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Carbohydrates of pigeon milk and their changes in the first week of secretion. 鸽奶中碳水化合物及其在第一周分泌时的变化。

Archives internationales de physiologie, de biochimie et de biophysique Pub Date : 1994-09-01 DOI: 10.3109/13813459409003944

S Shetty, P V Salimath, S N Hegde

{"title":"Carbohydrates of pigeon milk and their changes in the first week of secretion.","authors":"S Shetty, P V Salimath, S N Hegde","doi":"10.3109/13813459409003944","DOIUrl":"https://doi.org/10.3109/13813459409003944","url":null,"abstract":"The carbohydrate content of pigeon crop secretion called pigeon milk (PM) was in the range of 0.9-1.5%. Sugars of trichloroacetic acid soluble (TCA-S) fraction increased by 67% between day-1 and day-5 of secretion while those of TCA- insoluble (TCA-P) fraction remained fairly constant. Sialic acids constituted 5-9% of carbohydrates. The proportion of lipid- and protein-bound sialic acids was 51% and 31% respectively; the former increased from 41% to 68% between day-1 and day-5 whereas the latter decreased from 45% to 21% during the corresponding period. Some of the sugars of PM were fucose (40%), glucosamine (31%), galactose (12%), mannose (9%) and glucose (8%). The free sugars whose content was very low (0.05%) included fucose, mannose, glucose and some unidentified oligosaccharides. The proportion of lipid- and protein-bound sugars was 31% and 63% respectively; the former decreased by 7% from day-1 to day-5 while the latter increased by 11% during the same period. Gel chromatography of PM confirmed the presence of sialic acids and glucosamine; the latter existed both in free and bound form. The nature of changes in the carbohydrate composition of PM in the first week of secretion was more quantitative than qualitative.","PeriodicalId":77008,"journal":{"name":"Archives internationales de physiologie, de biochimie et de biophysique","volume":"102 5","pages":"277-80"},"PeriodicalIF":0.0,"publicationDate":"1994-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813459409003944","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18847329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Comparison of macrophage phospholipid radiolabelling methods for measuring phospholipase A2 inhibition. 巨噬细胞磷脂放射性标记法测定磷脂酶A2抑制作用的比较。

Archives internationales de physiologie, de biochimie et de biophysique Pub Date : 1994-09-01 DOI: 10.3109/13813459409003943

J Y Petit, P Boucrot, F Lang, L Welin

{"title":"Comparison of macrophage phospholipid radiolabelling methods for measuring phospholipase A2 inhibition.","authors":"J Y Petit, P Boucrot, F Lang, L Welin","doi":"10.3109/13813459409003943","DOIUrl":"https://doi.org/10.3109/13813459409003943","url":null,"abstract":"Rat peritoneal macrophages activated by ionophore A 23187, were labelled after introduction in the culture medium of 1-0-stearoyl 2-0-[3H] arachidonylglycero-3-phosphocholine (as unique source of tritiated arachidonic acid), or [3H] arachidonic acid which was esterified by cells in phospholipids and triglycerides or remained non esterified. With either cell-labelling method, stimulated macrophages produced tritiated nonesterified fatty acids and eicosanoids which were isolated from cell and medium lipids. When introduced into the culture medium at 1, 5 or 10 microM, the membrane phospholipid analogue 1,2 di-O-hexadecylglycerophosphocholine (dihexadecyl-GPC), but not the lysolecithin analogue 1-0-octadecyl 2-0-methylglycero phosphocholine, lowered phospholipase A2 activity with either labelling method. Dihexadecyl-GPC had an inhibitory effect on the release of arachidonic acid and eicosanoids. Moreover, this effect, as measured by tritiated nonesterified fatty acid formation, was greater in activated cells labelled with tritiated phospholipid (IC50 6 microM) than with [3H] arachidonic acid (IC50 60 microM). This is attributable to the inhibitory effect of dihexadecyl-GPC on endogenous phospholipase A2 and the endogenous enzyme excreted together with lysosomes into the medium. It may be concluded that radioactive phospholipid labelling is a sensitive method for measuring phospholipase A2 activity and assessing the effects of potential phospholipase inhibitors.","PeriodicalId":77008,"journal":{"name":"Archives internationales de physiologie, de biochimie et de biophysique","volume":"102 5","pages":"271-5"},"PeriodicalIF":0.0,"publicationDate":"1994-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813459409003943","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18847803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

[Role of NO in cardiovascular and respiratory physiology]. NO在心血管和呼吸生理中的作用。

Archives internationales de physiologie, de biochimie et de biophysique Pub Date : 1994-07-01 DOI: 10.3109/13813459109045390

A T Dinh-Xuan

{"title":"[Role of NO in cardiovascular and respiratory physiology].","authors":"A T Dinh-Xuan","doi":"10.3109/13813459109045390","DOIUrl":"https://doi.org/10.3109/13813459109045390","url":null,"abstract":"Nitric oxide (NO) is now considered as the endogenous nitrovasodilator which is mainly derived from vascular endothelial cells in physiological conditions. Biosynthesis of NO is controlled by a family of enzymes, the NO synthases (NOS), that can be divided into two major subgroups, namely the constitutive and the inducible NOS. The constitutive NOS is the principal isoform found in endothelial cells. Endothelial dysfunction, as seen in chronic hypoxic lung diseases, impairs endogenous production of NO, thereby causing and/or aggravating pulmonary hypertension. A logical means to reduce pulmonary hypertension would consist in supplying the patients with exogenous NO. Given by inhalation, NO is a selective pulmonary vasodilator, as it rapidly combines with haemoglobin, which inactivates NO, and therefore prevents the occurrence of systemic hypotension. Endothelial dysfunction resulting in reduced NO synthesis is also likely to account for various cardiovascular disorders, including essential hypertension and coronary atherosclerosis. However, the importance of endogenous NO in the modulation of bronchial tone remains to be established. Current investigations include studies looking at regulatory mechanisms of cellular expression of various NOS isoforms on the one hand and, on the other hand, clinical evaluation of short- and long-term inhalation of NO in patients with primary and secondary pulmonary hypertension.","PeriodicalId":77008,"journal":{"name":"Archives internationales de physiologie, de biochimie et de biophysique","volume":"102 4","pages":"A3-9"},"PeriodicalIF":0.0,"publicationDate":"1994-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813459109045390","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18998414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

[Autoregulation of body weight and body composition in man. A systematic approach through models and simulation]. 人体体重和身体成分的自动调节。通过模型和仿真的系统方法]。

Archives internationales de physiologie, de biochimie et de biophysique Pub Date : 1994-07-01 DOI: 10.3109/13813459109045389

L Girardier

{"title":"[Autoregulation of body weight and body composition in man. A systematic approach through models and simulation].","authors":"L Girardier","doi":"10.3109/13813459109045389","DOIUrl":"https://doi.org/10.3109/13813459109045389","url":null,"abstract":"The regulation of body weight and its composition in humans is examined from the standpoint of system analysis. It is based upon the hypothesis that the ultimate purpose for modulating body energy stores is to ensure the maximum duration for survival of the individual in the face of calorie deficit. A mathematical model, which is built on this hypothesis, allows quantitative simulation of variations in body weight and composition which occur during cycles of underfeeding/refeeding, and is achieved with the use of three global parameters. One of them controls the partitioning of body energy stores between fat and lean tissues, and the other two are concerned with the control of adaptive thermogenesis. The first of these two parameters for thermogenesis modulates the efficiency of fat storage by a function which is inversely proportional to the level of replenishment of the adipose tissues, and the other one controls regulatory thermogenesis which is a direct function of energy imbalance. These two forms of adaptive thermogenesis operate independently of each other and their amplitude depends upon the nutritional background of the individual. This model can be used as a theoretical framework for integrating numerous data in the literature on physiological processes that are involved in the regulation of body weight and body composition.","PeriodicalId":77008,"journal":{"name":"Archives internationales de physiologie, de biochimie et de biophysique","volume":"102 4","pages":"A23-35"},"PeriodicalIF":0.0,"publicationDate":"1994-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813459109045389","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18998417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9