The Penn dental journal最新文献_第7页

Expression of cytolethal distending toxin genes from actinobacillus actinomycetemcomitans in escherichia coli. 放线菌膨胀毒素基因在大肠杆菌中的表达。

The Penn dental journal Pub Date : 2001-01-01

P A Sansaricq, K Mao, C J DiRienzo

引用次数: 0

Reliability of CCD and CMOS (APS) digital sensors compared with D and E-plus-speed films in the detection of dental pathology: an in vitro study. CCD和CMOS (APS)数字传感器与D和e -plus速度膜在口腔病理检测中的可靠性比较:体外研究。

The Penn dental journal Pub Date : 2001-01-01

J N Tsau, M Mupparapu

引用次数: 0

The new F-speed films in dentistry: a brief review. 新的f -速度胶片在牙科:一个简短的回顾。

The Penn dental journal Pub Date : 2001-01-01

M Mupparapu, E Mozaffari

引用次数: 0

Search for an endogenous collagenase in chicken endochondral bone matrix vesicles. 鸡软骨内骨基质囊泡内源性胶原酶的研究。

The Penn dental journal Pub Date : 2001-01-01

D Chen, E E Golub

{"title":"Search for an endogenous collagenase in chicken endochondral bone matrix vesicles.","authors":"D Chen, E E Golub","doi":"","DOIUrl":"","url":null,"abstract":"While isolating matrix vesicles from avian endochondral bone, collagenase activity was discovered unexpectedly. The question was raised whether this collagenase activity was endogenous or if it was due to the bacterial collagenase used to release the matrix vesicles from bone. In related experiments done, collagen partially degraded by collagenase mineralized better than undegraded collagen. This study would then attempt to show if an endogenous collagenase is found, whether it facilitates collagen mineralization by allowing better access for matrix vesicles by the \"nicking\" of collagen. From this two-month study evidence was found that an endogenous collagenase as well as a gelatinase was present on matrix vesicles. SDS gel electrophoresis and zymography were used to determine the presence of collagenases in purified matrix vesicle fractions. Western blots with antibodies to bacterial collagenase, matrix metallo-proteinases 13 (MMP-13), an endogenous collagenase, and MMP-2, an endogenous gelatinase, were also done to determine the presence of an endogenous collagenase. Another facet was added to this study to determine if contamination by exogenous collagenase during matrix vesicle isolation could be removed. From the final Western blots done, it was verified that even after exhaustive washings of the column and centrifugation, purified matrix vesicles contained contaminating bacterial collagenase. Due to the research completed, the matrix vesicle isolation protocol was changed from using bacterial collagenase to a trypsin isolation method to prevent any exogenous collagenase contamination.","PeriodicalId":76715,"journal":{"name":"The Penn dental journal","volume":"101 ","pages":"12-3, 32"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40997206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial organization and biofilm colonization of viridans streptococci. 翠绿链球菌的空间组织和生物膜定植。

The Penn dental journal Pub Date : 2001-01-01

R Wu

引用次数: 0

Alumni profile: life is. 校友简介:人生是。

The Penn dental journal Pub Date : 2001-01-01

H E Goldstein

引用次数: 0

Analysis of the human periodontal ligament fibroblast response to the cytolethal distending toxin produced by actinobacillus actinomycetemcomitans. 人牙周韧带成纤维细胞对放线菌产生的细胞致死膨胀毒素的反应分析。

The Penn dental journal Pub Date : 2001-01-01

R Lukasiewicz

{"title":"Analysis of the human periodontal ligament fibroblast response to the cytolethal distending toxin produced by actinobacillus actinomycetemcomitans.","authors":"R Lukasiewicz","doi":"","DOIUrl":"","url":null,"abstract":"A small number of diverse bacterial pathogens have been found to produce cytolethal distending toxin (CDT). Much evidence has been obtained to show that CDTs cause a variety of eukaryotic cells to become irreversibly blocked in the G2 phase of the cell cycle. Cells which are sensitive to CDT, such as Chinese hamster ovary (CHO) cells, die within a few days after exposure to the toxin. Recent research has shown that CDT is a potential virulence factor for Actinobacillus actinomycetemcomitans, a bacterial species associated with juvenile periodontitis (JP). This research investigates the effects A. actinomycetemcomitans CDT has on human periodontal ligament fibroblasts (HPDLFs). After HPDLFs were exposed to physiologic levels of CDT, cell viability was assessed utilizing an assay that measured the conversion of a tetrazolium compound into a soluble formazan product, which could be read on a spectrophotometer. Cell viability was also measured after exposure to CDT through trypan blue staining and the use of a hemocytometer. CHO cells were utilized as a parallel control to show that the CDT obtained from A. actinomycetemcomitans was active. The results obtained indicate that the HPDLFs are resistant to the cytolethal distending toxin. The possible resistance the HPDLFs have to the CDT may stem from the low turnover rate of the fibroblasts. However, further research must be completed on this topic.","PeriodicalId":76715,"journal":{"name":"The Penn dental journal","volume":"101 ","pages":"6-7, 32"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40997202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interleukin 4 in primary Sjören's syndrome. 白细胞介素4在原发性Sjören综合征中的作用。

The Penn dental journal Pub Date : 2001-01-01

J Ramirez

引用次数: 0

Management of the child with neurofibromatosis Type I, von Recklinghausen Disease. 1型神经纤维瘤病儿童的治疗。

The Penn dental journal Pub Date : 2001-01-01

A Cheifetz

引用次数: 0

Dentistry: changing with the ages. 牙科:随着年龄的变化而变化。

The Penn dental journal Pub Date : 1996-01-01

S L Chin, C Estilo

引用次数: 0