Aging (Albany NY)最新文献

Correction for: Inhibition of circulating exosomal microRNA-15a-3p accelerates diabetic wound repair 更正抑制循环外泌体microRNA-15a-3p可加速糖尿病伤口修复

Aging (Albany NY) Pub Date : 2024-04-15 DOI: 10.18632/aging.205514

Y. Xiong, Lang Chen, Tao Yu, Chenchen Yan, Wu Zhou, Faqi Cao, Xiaomeng You, Yingqi Zhang, Yun Sun, Jing Liu, Hang Xue, Yiqiang Hu, Dong Chen, B. Mi, Guohui Liu

引用次数: 0

Correction for: MTDH promotes metastasis of clear cell renal cell carcinoma by activating SND1-mediated ERK signaling and epithelial-mesenchymal transition 更正为MTDH 通过激活 SND1 介导的 ERK 信号转导和上皮-间质转化促进透明细胞肾细胞癌的转移

Aging (Albany NY) Pub Date : 2024-04-15 DOI: 10.18632/aging.205603

Anbang He, S. He, Cong Huang, Zhicong Chen, Yucai Wu, Y. Gong, Xuesong Li, Liqun Zhou

引用次数: 0

Machine learning for identifying tumor stemness genes and developing prognostic model in gastric cancer 利用机器学习识别胃癌肿瘤干性基因并开发预后模型

Aging (Albany NY) Pub Date : 2024-04-12 DOI: 10.18632/aging.205715

Guo-Xing Li, Yun-Peng Chen, You-Yang Hu, Wen-Jing Zhao, Yun-Yan Lu, Fu-Jian Wan, Zhi-Jun Wu, Xiang-Qian Wang, Qi-Ying Yu

{"title":"Machine learning for identifying tumor stemness genes and developing prognostic model in gastric cancer","authors":"Guo-Xing Li, Yun-Peng Chen, You-Yang Hu, Wen-Jing Zhao, Yun-Yan Lu, Fu-Jian Wan, Zhi-Jun Wu, Xiang-Qian Wang, Qi-Ying Yu","doi":"10.18632/aging.205715","DOIUrl":"https://doi.org/10.18632/aging.205715","url":null,"abstract":"Gastric cancer presents a formidable challenge, marked by its debilitating nature and often dire prognosis. Emerging evidence underscores the pivotal role of tumor stem cells in exacerbating treatment resistance and fueling disease recurrence in gastric cancer. Thus, the identification of genes contributing to tumor stemness assumes paramount importance. Employing a comprehensive approach encompassing ssGSEA, WGCNA, and various machine learning algorithms, this study endeavors to delineate tumor stemness key genes (TSKGs). Subsequently, these genes were harnessed to construct a prognostic model, termed the Tumor Stemness Risk Genes Prognostic Model (TSRGPM). Through PCA, Cox regression analysis and ROC curve analysis, the efficacy of Tumor Stemness Risk Scores (TSRS) in stratifying patient risk profiles was underscored, affirming its ability as an independent prognostic indicator. Notably, the TSRS exhibited a significant correlation with lymph node metastasis in gastric cancer. Furthermore, leveraging algorithms such as CIBERSORT to dissect immune infiltration patterns revealed a notable association between TSRS and monocytes and other cell. Subsequent scrutiny of tumor stemness risk genes (TSRGs) culminated in the identification of CDC25A for detailed investigation. Bioinformatics analyses unveil CDC25A’s implication in driving the malignant phenotype of tumors, with a discernible impact on cell proliferation and DNA replication in gastric cancer. Noteworthy validation through in vitro experiments corroborated the bioinformatics findings, elucidating the pivotal role of CDC25A expression in modulating tumor stemness in gastric cancer. In summation, the established and validated TSRGPM holds promise in prognostication and delineation of potential therapeutic targets, thus heralding a pivotal stride towards personalized management of this malignancy.","PeriodicalId":7669,"journal":{"name":"Aging (Albany NY)","volume":"8 16","pages":"6455 - 6477"},"PeriodicalIF":0.0,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140710074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Garlic oil supplementation blocks inflammatory pyroptosis-related acute lung injury by suppressing the NF-κB/NLRP3 signaling pathway via H2S generation 补充大蒜油可通过产生 H2S 抑制 NF-κB/NLRP3 信号通路，从而阻断与炎症性脓毒症相关的急性肺损伤

Aging (Albany NY) Pub Date : 2024-04-12 DOI: 10.18632/aging.205721

Tursunay Dilxat, Qiang Shi, Xiaofan Chen, Xuxin Liu

{"title":"Garlic oil supplementation blocks inflammatory pyroptosis-related acute lung injury by suppressing the NF-κB/NLRP3 signaling pathway via H2S generation","authors":"Tursunay Dilxat, Qiang Shi, Xiaofan Chen, Xuxin Liu","doi":"10.18632/aging.205721","DOIUrl":"https://doi.org/10.18632/aging.205721","url":null,"abstract":"Acute lung injury (ALI) is a major cause of acute respiratory failure with a high morbidity and mortality rate, and effective therapeutic strategies for ALI remain limited. Inflammatory response is considered crucial for the pathogenesis of ALI. Garlic, a globally used cooking spice, reportedly exhibits excellent anti-inflammatory bioactivity. However, protective effects of garlic against ALI have never been reported. This study aimed to investigate the protective effects of garlic oil (GO) supplementation on lipopolysaccharide (LPS)-induced ALI models. Hematoxylin and eosin staining, pathology scores, lung myeloperoxidase (MPO) activity measurement, lung wet/dry (W/D) ratio detection, and bronchoalveolar lavage fluid (BALF) analysis were performed to investigate ALI histopathology. Real-time polymerase chain reaction, western blotting, and enzyme-linked immunosorbent assay were conducted to evaluate the expression levels of inflammatory factors, nuclear factor-κB (NF-κB), NLRP3, pyroptosis-related proteins, and H2S-producing enzymes. GO attenuated LPS-induced pulmonary pathological changes, lung W/D ratio, MPO activity, and inflammatory cytokines in the lungs and BALF. Additionally, GO suppressed LPS-induced NF-κB activation, NLRP3 inflammasome expression, and inflammatory-related pyroptosis. Mechanistically, GO promoted increased H2S production in lung tissues by enhancing the conversion of GO-rich polysulfide compounds or by increasing the expression of H2S-producing enzymes in vivo. Inhibition of endogenous or exogenous H2S production reversed the protective effects of GO on ALI and eliminated the inhibitory effects of GO on NF-κB, NLRP3, and pyroptotic signaling pathways. Overall, these findings indicate that GO has a critical anti-inflammatory effect and protects against LPS-induced ALI by suppressing the NF-κB/NLRP3 signaling pathway via H2S generation.","PeriodicalId":7669,"journal":{"name":"Aging (Albany NY)","volume":"17 23","pages":"6521 - 6536"},"PeriodicalIF":0.0,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140712044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NXPH4 can be used as a biomarker for pan-cancer and promotes colon cancer progression NXPH4 可用作泛癌症的生物标记物，并促进结肠癌的进展

Aging (Albany NY) Pub Date : 2024-04-12 DOI: 10.18632/aging.205648

Zhipeng Zhang, Pengfei Wang, Siwen Chen, Dezhi Xiang, Jinzhen Chen, Wanchang Huang, Xiao Liu, Tongwen Yi, Dawei Wang, Yunfei Pu, Longfu He, Hao Zhang

{"title":"NXPH4 can be used as a biomarker for pan-cancer and promotes colon cancer progression","authors":"Zhipeng Zhang, Pengfei Wang, Siwen Chen, Dezhi Xiang, Jinzhen Chen, Wanchang Huang, Xiao Liu, Tongwen Yi, Dawei Wang, Yunfei Pu, Longfu He, Hao Zhang","doi":"10.18632/aging.205648","DOIUrl":"https://doi.org/10.18632/aging.205648","url":null,"abstract":"NXPH4 promotes cancer proliferation and invasion. However, its specific role and mechanism in cancer remain unclear. Transcriptome and clinical data for pan-cancer were derived from the TCGA database. K-M survival curve and univariate Cox were used for prognostic analysis. CIBERSORT and TIMER algorithms were employed to calculate immune cell infiltration. Gene set enrichment analysis (GSEA) was employed for investigating the function of NXPH4. Western blot verified differential expression of NXPH4 in colon cancer. Functional assays (CCK-8, plate clonogenicity assay, wound healing assay, and Transwell assay) confirmed the impact of NXPH4 on proliferation, invasion, and migration of colon cancer cells. Dysregulation of NXPH4 in pan-cancer suggests its potential as a diagnostic and prognostic marker for certain cancers, including colon and liver cancer. High expression of NXPH4 in pan-cancer might be associated with the increase in copy number and hypomethylation. NXPH4 expression in pan-cancer is substantially linked to immune cell infiltration in the immune microenvironment. NXPH4 expression is associated with the susceptibility to immunotherapy and chemotherapy. Western blot further confirmed the higher expression of NXPH4 in colon cancer. Knockdown of NXPH4 significantly suppresses proliferation, invasion, and migration of colon cancer cell lines HT-29 and HCT116, as validated by functional assays.","PeriodicalId":7669,"journal":{"name":"Aging (Albany NY)","volume":"4 7","pages":"5866 - 5886"},"PeriodicalIF":0.0,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140710789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of continuous and intermittent ketogenic diets on cognitive behavior, motor function, and blood lipids in TgF344-AD rats 持续性和间歇性生酮饮食对 TgF344-AD 大鼠认知行为、运动功能和血脂的影响

Aging (Albany NY) Pub Date : 2024-04-12 DOI: 10.18632/aging.205741

J. Rutkowsky, Zabrisky Roland, Anthony Valenzuela, An B Nguyen, Heui Hye Park, Natalie Six, Ilknur Dursun, Kyoungmi Kim, Pamela J Lein, Jon J Ramsey

{"title":"The impact of continuous and intermittent ketogenic diets on cognitive behavior, motor function, and blood lipids in TgF344-AD rats","authors":"J. Rutkowsky, Zabrisky Roland, Anthony Valenzuela, An B Nguyen, Heui Hye Park, Natalie Six, Ilknur Dursun, Kyoungmi Kim, Pamela J Lein, Jon J Ramsey","doi":"10.18632/aging.205741","DOIUrl":"https://doi.org/10.18632/aging.205741","url":null,"abstract":"Studies suggest that ketogenic diets (KD) may improve memory in mouse models of aging and Alzheimer’s disease (AD). This study determined whether a continuous or intermittent KD (IKD) enhanced cognitive behavior in the TgF344-AD rat model of AD. At 6 months-old, TgF344-AD and wild-type (WT) littermates were placed on a control (CD), KD, or IKD (morning CD and afternoon KD) provided as two meals per day for 2 or 6 months. Cognitive and motor behavior and circulating β-hydroxybutyrate (BHB), AD biomarkers and blood lipids were assessed. Animals on a KD diet had elevated circulating BHB, with IKD levels intermediate to CD and KD. TgF344-AD rats displayed impaired spatial learning memory in the Barnes maze at 8 and 12 months of age and impaired motor coordination at 12 months of age. Neither KD nor IKD improved performance compared to CD. At 12 months of age, TgF344-AD animals had elevated blood lipids. IKD reduced lipids to WT levels with KD further reducing cholesterol below WT levels. This study shows that at 8 or 12 months of age, KD or IKD intervention did not improve measures of cognitive or motor behavior in TgF344-AD rats; however, both IKD and KD positively impacted circulating lipids.","PeriodicalId":7669,"journal":{"name":"Aging (Albany NY)","volume":"123 21","pages":"5811 - 5828"},"PeriodicalIF":0.0,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140708849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mechanism of USP43 in the development of tumor: a literature review USP43 在肿瘤发生中的作用机制：文献综述

Aging (Albany NY) Pub Date : 2024-04-08 DOI: 10.18632/aging.205731

Ziqi Zhao, Meichen Liu, Zhikun Lin, Mengru Zhu, Linlin Lv, Xinqing Zhu, Rui Fan, Abdullah Al-danakh, Hui He, Guang Tan

引用次数: 0

Single-cell data revealed exhaustion of characteristic NK cell subpopulations and T cell subpopulations in hepatocellular carcinoma 单细胞数据显示肝细胞癌中特征性 NK 细胞亚群和 T 细胞亚群已衰竭

Aging (Albany NY) Pub Date : 2024-04-05 DOI: 10.18632/aging.205723

Zhong-Ning Cui, Hongzhi Li, Chunli Liu, Juan Wang, Chunguang Chen, Shanlei Hu, Xiaoli Zhao, Guangming Li

{"title":"Single-cell data revealed exhaustion of characteristic NK cell subpopulations and T cell subpopulations in hepatocellular carcinoma","authors":"Zhong-Ning Cui, Hongzhi Li, Chunli Liu, Juan Wang, Chunguang Chen, Shanlei Hu, Xiaoli Zhao, Guangming Li","doi":"10.18632/aging.205723","DOIUrl":"https://doi.org/10.18632/aging.205723","url":null,"abstract":"Background: The treatment and prognosis of patients with advanced hepatocellular carcinoma (HCC) have been a major medical challenge. Unraveling the landscape of tumor immune infiltrating cells (TIICs) in the immune microenvironment of HCC is of great significance to probe the molecular mechanisms. Methods: Based on single-cell data of HCC, the cell landscape was revealed from the perspective of TIICs. Special cell subpopulations were determined by the expression levels of marker genes. Differential expression analysis was conducted. The activity of each subpopulation was determined based on the highly expressed genes. CTLA4+ T-cell subpopulations affecting the prognosis of HCC were determined based on survival analysis. A single-cell regulatory network inference and clustering analysis was also performed to determine the transcription factor regulatory networks in the CTLA4+ T cell subpopulations. Results: 10 cell types were identified and NK cells and T cells showed high abundance in tumor tissues. Two NK cells subpopulations were present, FGFBP2+ NK cells, B3GNT7+ NK cells. Four T cells subpopulations were present, LAG3+ T cells, CTLA4+ T cells, RCAN3+ T cells, and HPGDS+ Th2 cells. FGFBP2+ NK cells, and CTLA4+ T cells were the exhaustive subpopulation. High CTLA4+ T cells contributed to poor prognostic outcomes and promoted tumor progression. Finally, a network of transcription factors regulated by NR3C1, STAT1, and STAT3, which were activated, was present in CTLA4+ T cells. Conclusion: CTLA4+ T cell subsets in HCC exhibited functional exhaustion characteristics that probably inhibited T cell function through a transcription factor network dominated by NR3C1, STAT1, and STAT3.","PeriodicalId":7669,"journal":{"name":"Aging (Albany NY)","volume":"3 11","pages":"6550 - 6565"},"PeriodicalIF":0.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140735983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TGF-β downstream of Smad3 and MAPK signaling antagonistically regulate the viability and partial epithelial–mesenchymal transition of liver progenitor cells TGF-β下游的Smad3和MAPK信号拮抗调控肝祖细胞的活力和部分上皮-间质转化

Aging (Albany NY) Pub Date : 2024-04-05 DOI: 10.18632/aging.205725

Yi-min Sun, Yu Wu, Gan-xun Li, Hui-Fang Liang, Tu-Ying Yong, Zifu Li, Bixiang Zhang, Xiao-Ping Chen, Guan-nan Jin, Ze-Yang Ding

{"title":"TGF-β downstream of Smad3 and MAPK signaling antagonistically regulate the viability and partial epithelial–mesenchymal transition of liver progenitor cells","authors":"Yi-min Sun, Yu Wu, Gan-xun Li, Hui-Fang Liang, Tu-Ying Yong, Zifu Li, Bixiang Zhang, Xiao-Ping Chen, Guan-nan Jin, Ze-Yang Ding","doi":"10.18632/aging.205725","DOIUrl":"https://doi.org/10.18632/aging.205725","url":null,"abstract":"Background: Liver progenitor cells (LPCs) are a subpopulation of cells that contribute to liver regeneration, fibrosis and liver cancer initiation under different circumstances. Results: By performing adenoviral-mediated transfection, CCK-8 analyses, F-actin staining, transwell analyses, luciferase reporter analyses and Western blotting, we observed that TGF-β promoted cytostasis and partial epithelial–mesenchymal transition (EMT) in LPCs. In addition, we confirmed that TGF-β activated the Smad and MAPK pathways, including the Erk, JNK and p38 MAPK signaling pathways, and revealed that TGFβ-Smad signaling induced growth inhibition and partial EMT, whereas TGFβ-MAPK signaling had the opposite effects on LPCs. We further found that the activity of Smad and MAPK signaling downstream of TGF-β was mutually restricted in LPCs. Mechanistically, we found that TGF-β activated Smad signaling through serine phosphorylation of both the C-terminal and linker regions of Smad2 and 3 in LPCs. Additionally, TGFβ-MAPK signaling inhibited the phosphorylation of Smad3 but not Smad2 at the C-terminus, and it reinforced the linker phosphorylation of Smad3 at T179 and S213. We then found that overexpression of mutated Smad3 at linker phosphorylation sites intensifies TGF-β-induced cytostasis and EMT, mimicking the effects of MAPK inhibition in LPCs, whereas mutation of Smad3 at the C-terminus caused LPCs to blunt TGF-β-induced cytostasis and partial EMT. Conclusion: These results suggested that TGF-β downstream of Smad3 and MAPK signaling were mutually antagonistic in regulating the viability and partial EMT of LPCs. This antagonism may help LPCs overcome the cytostatic effect of TGF-β under fibrotic conditions and maintain partial EMT and progenitor phenotypes.","PeriodicalId":7669,"journal":{"name":"Aging (Albany NY)","volume":"29 6","pages":"6588 - 6612"},"PeriodicalIF":0.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140738347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A prognostic signature established based on genes related to tumor microenvironment for patients with hepatocellular carcinoma 基于肿瘤微环境相关基因的肝细胞癌患者预后特征的建立

Aging (Albany NY) Pub Date : 2024-04-04 DOI: 10.18632/aging.205722

Zhong-Ning Cui, Ge Li, Yanbin Shi, Xiaoli Zhao, Juan Wang, Shanlei Hu, Chunguang Chen, Guangming Li

{"title":"A prognostic signature established based on genes related to tumor microenvironment for patients with hepatocellular carcinoma","authors":"Zhong-Ning Cui, Ge Li, Yanbin Shi, Xiaoli Zhao, Juan Wang, Shanlei Hu, Chunguang Chen, Guangming Li","doi":"10.18632/aging.205722","DOIUrl":"https://doi.org/10.18632/aging.205722","url":null,"abstract":"Background: Complex cellular signaling network in the tumor microenvironment (TME) could serve as an indicator for the prognostic classification of hepatocellular carcinoma (HCC) patients. Methods: Univariate Cox regression analysis was performed to screen prognosis-related TME-related genes (TRGs), based on which HCC samples were clustered by running non-negative matrix factorization (NMF) algorithm. Furthermore, the correlation between different molecular HCC subtypes and immune cell infiltration level was analyzed. Finally, a risk score (RS) model was established by LASSO and Cox regression analyses (CRA) using these TRGs. Functional enrichment analysis was performed using gene set enrichment analysis (GSEA). Results: HCC patients were divided into three molecular subtypes (C1, C2, and C3) based on 704 prognosis-related TRGs. HCC subtype C1 had significantly better OS than C2 and C3. We selected 13 TRGs to construct the RS model. Univariate and multivariate CRA showed that the RS could independently predict patients’ prognosis. A nomogram integrating the RS and clinicopathologic features of the patients was further created. We also validated the reliability of the model according to the area under the receiver operating characteristic (ROC) curve value, concordance index (C-index), and decision curve analysis. The current findings demonstrated that the RS was significantly correlated with CD8+ T cells, monocytic lineage, and myeloid dendritic cells. Conclusion: This study provided TRGs to help classify patients with HCC and predict their prognoses, contributing to personalized treatments for patients with HCC.","PeriodicalId":7669,"journal":{"name":"Aging (Albany NY)","volume":"14 6","pages":"6537 - 6549"},"PeriodicalIF":0.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140741809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0