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Quaderni Sclavo di diagnostica clinica e di laboratorio最新文献

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[Isolation frequency of enteropathogenic Escherichia coli in the Patro distric from 1974 to 1986]. [1974 - 1986年Patro地区肠致病性大肠杆菌的分离频率]。
Quaderni Sclavo di diagnostica clinica e di laboratorio Pub Date : 1988-01-01
B M Casadei, A Limberti, A M Ciabatti, L Livatino, C Ignesti
{"title":"[Isolation frequency of enteropathogenic Escherichia coli in the Patro distric from 1974 to 1986].","authors":"B M Casadei,&nbsp;A Limberti,&nbsp;A M Ciabatti,&nbsp;L Livatino,&nbsp;C Ignesti","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In the Prato district in the years 1974-1986 the isolation frequency of serogropus of enteropathogenic Escherichia coli on 12,251 faeces samples pertinent to infantile diarrhoea has been studied. The research reveals a constant decrease in the isolation frequency of the serogroups considered. An hypothesis is quite difficult but this finding is taking world-wide consistency: furthermore some items are distinctive of the researched district and therefore quite interesting.</p>","PeriodicalId":76395,"journal":{"name":"Quaderni Sclavo di diagnostica clinica e di laboratorio","volume":"24 1-4","pages":"45-53"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14209369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Possibility of early diagnosis of hepatic fibrosis in chronic alcoholics determining the N-terminal peptide of type-III procollagen]. [检测iii型前胶原n端肽对慢性酗酒者肝纤维化早期诊断的可能性]。
Quaderni Sclavo di diagnostica clinica e di laboratorio Pub Date : 1988-01-01
R Ricciardi, G Restuccia
{"title":"[Possibility of early diagnosis of hepatic fibrosis in chronic alcoholics determining the N-terminal peptide of type-III procollagen].","authors":"R Ricciardi,&nbsp;G Restuccia","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The authors examined the behaviour of sP-III-P and other parameters in 40 patients affected by alcoholic liver disease (20 of whom with steatosis, 10 with steatofibrosis, 10 with liver cirrhosis) and at the same time in a group of non drinking controls. The levels of sP-III-P appeared significantly increased in the patients with steato-fibrosis and even more so in those with liver cirrhosis, while in those with steatosis the increase was insignificant. The gamma-globulins showed similar behaviour while serum albumin appeared proportionally reduced in the cases in which sP-III-P and gamma-globulins showed a greater increase. The authors believe that the study of sP-III-P is valuable in the follow-up of patients with alcoholic liver disease and in particular in chronic alcoholism to reveal the presence of an increased fibrogenesis.</p>","PeriodicalId":76395,"journal":{"name":"Quaderni Sclavo di diagnostica clinica e di laboratorio","volume":"24 1-4","pages":"171-8"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14209562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Application to an automatic system of a direct method for determining atypical variants of cholinesterase using succinyldithiocholine as substrate]. [以琥珀酰二硫代胆碱为底物测定胆碱酯酶非典型变异的直接法自动系统的应用]。
Quaderni Sclavo di diagnostica clinica e di laboratorio Pub Date : 1988-01-01
G Capone, D Gnudi, L Pensabeni, A M Martelli, M C Marconi, A Accorsi
{"title":"[Application to an automatic system of a direct method for determining atypical variants of cholinesterase using succinyldithiocholine as substrate].","authors":"G Capone,&nbsp;D Gnudi,&nbsp;L Pensabeni,&nbsp;A M Martelli,&nbsp;M C Marconi,&nbsp;A Accorsi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The determination of human serum cholinesterase (EC 3.1.1.8) is frequently requested to detect patients with atypical forms of the enzyme which reacts abnormally with succinylcholine (suxamethonium), employed as a neuromuscular blocking agent. Usually, for biochemical identification of succinylcholine sensitive individuals the standard reaction is run with and without the inhibitors, notably dibucaine. A new test for direct determination of succinylcholine sensitive individuals which use a substrate analogue of succinylcholine was applied to a automated instrument. The linearity, precision, recovery, interference and correlation of the method have been evaluated. We have estimated the reference intervals for a population of 364 healthy subjects subdivided for sex, three atypical homozygotes and two atypical heterozygotes. On the basis of analytical performance we can conclude that this test may offer a further parameter for preoperative screening of individuals with an abnormal response to the muscle relaxant succinylcholine, thus avoiding the determination of genotype by measurement of dibucaine number.</p>","PeriodicalId":76395,"journal":{"name":"Quaderni Sclavo di diagnostica clinica e di laboratorio","volume":"24 1-4","pages":"189-96"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14395137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Relation between fasting C-peptide and other clinical and metabolic variables in non-insulin-dependent diabetes mellitus. 非胰岛素依赖型糖尿病空腹c肽与其他临床及代谢变量的关系
Quaderni Sclavo di diagnostica clinica e di laboratorio Pub Date : 1988-01-01
G Seghieri, L Alviggi, P Caselli, C Breschi, P Mammini, A Gironi, A Bartolomei, L A De Giorgio
{"title":"Relation between fasting C-peptide and other clinical and metabolic variables in non-insulin-dependent diabetes mellitus.","authors":"G Seghieri,&nbsp;L Alviggi,&nbsp;P Caselli,&nbsp;C Breschi,&nbsp;P Mammini,&nbsp;A Gironi,&nbsp;A Bartolomei,&nbsp;L A De Giorgio","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76395,"journal":{"name":"Quaderni Sclavo di diagnostica clinica e di laboratorio","volume":"24 1-4","pages":"65-72"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14395906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Determination of serum bilirubin fractions using gel-filtration]. [凝胶过滤法测定血清胆红素含量]。
Quaderni Sclavo di diagnostica clinica e di laboratorio Pub Date : 1988-01-01
C Franzini, G Cattozzo, A Pagani
{"title":"[Determination of serum bilirubin fractions using gel-filtration].","authors":"C Franzini,&nbsp;G Cattozzo,&nbsp;A Pagani","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Serum bilirubin has been fractionated by means of a gel-filtration technique, on Bio-Gel P-10 columns and with caffeine solution as eluent. When sera containing high concentration of direct-reacting bilirubin were applied to the column, a bilirubin fraction was eluted with the protein. This bilirubin fraction is not set free from protein in the presence of urea, guanidine and sodium dodecyl sulfate; moreover it exhibits peculiar behaviour in the direct and indirect diazo-reaction and in enzymatic oxidation by bilirubin-oxidase as well. On the basis of these data, such bilirubin fraction has been identified with the previously described delta-bilirubin. A method for the quantitative measurement of delta-bilirubin, based on gel-filtration separation followed by diazo-reaction, has been worked out. Results by this method satisfactorily compared with the Kodak Ektachem method.</p>","PeriodicalId":76395,"journal":{"name":"Quaderni Sclavo di diagnostica clinica e di laboratorio","volume":"24 1-4","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14397440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Hemolytic group B streptococcus in cervico-vaginal secretions]. [宫颈阴道分泌物中的溶血性B组链球菌]。
Quaderni Sclavo di diagnostica clinica e di laboratorio Pub Date : 1988-01-01
A Savarino, R Mattei, A Barsotti, R Bechi, F Saisi, F Lenci
{"title":"[Hemolytic group B streptococcus in cervico-vaginal secretions].","authors":"A Savarino,&nbsp;R Mattei,&nbsp;A Barsotti,&nbsp;R Bechi,&nbsp;F Saisi,&nbsp;F Lenci","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The group B streptococcus can be an etiological agent of newborn's infections and vaginitis. Therefore, we evaluated the statistical incidence of this microorganism in vaginal swabs of 1403 non-selected subjects. We isolated this streptococcus in 12.3% samples and it was present in 26.3% of positive cultures. Our study confirms the elevate incidence of this microorganism and suggests the opportunity of this research in pregnancy monitoring and in gynaecological flogosis.</p>","PeriodicalId":76395,"journal":{"name":"Quaderni Sclavo di diagnostica clinica e di laboratorio","volume":"24 1-4","pages":"185-8"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14209565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Evaluation of the specific immune response after anti-rubella vaccination]. [抗风疹疫苗接种后特异性免疫反应的评价]。
Quaderni Sclavo di diagnostica clinica e di laboratorio Pub Date : 1988-01-01
C Cellesi, A Rossolini, G M Rossolini, M L Bianchi Bandinelli, M G Cusi, P Egisto Valensin
{"title":"[Evaluation of the specific immune response after anti-rubella vaccination].","authors":"C Cellesi,&nbsp;A Rossolini,&nbsp;G M Rossolini,&nbsp;M L Bianchi Bandinelli,&nbsp;M G Cusi,&nbsp;P Egisto Valensin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A selective rubella immunization program was carried out in the Siena (Italy) area since 1984. 912 schoolgirls aged 10 to 18 were screened for rubella antibodies. Seropositivity rates, increasing with age, appeared quite heterogenous among subjects living in different villages. Seronegative girls were partly voluntarily immunized with RA 27/3 live attenuated rubella vaccine. Serological and clinical controls performed on 238 subjects one month after immunization showed a 99.6% vaccine-induced seroconversion rate and mild post-vaccinal reactions in 26% of vaccinees. Serological control performed, on 172 subjects, at 8-12 days after vaccination revealed that in most cases vaccine induced haemagglutination-inhibiting antibodies are not detectable at the time. The results are discussed in relation to a forthcoming introduction of compulsory rubella vaccination in our Country.</p>","PeriodicalId":76395,"journal":{"name":"Quaderni Sclavo di diagnostica clinica e di laboratorio","volume":"24 1-4","pages":"97-105"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14395904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A combination of Gomori-Grocott methenamine silver nitrate and hematoxylene and eosin staining technique for the demonstration of Candida albicans in tissue. Gomori-Grocott甲基亚胺硝酸银与苏木烯和伊红染色技术的结合显示组织中的白色念珠菌。
Quaderni Sclavo di diagnostica clinica e di laboratorio Pub Date : 1988-01-01
B K Sinha, D P Monga, S Prasad
{"title":"A combination of Gomori-Grocott methenamine silver nitrate and hematoxylene and eosin staining technique for the demonstration of Candida albicans in tissue.","authors":"B K Sinha,&nbsp;D P Monga,&nbsp;S Prasad","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76395,"journal":{"name":"Quaderni Sclavo di diagnostica clinica e di laboratorio","volume":"24 1-4","pages":"129-32"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13623532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Semiautomatic procedure for determining urine iron in subjects treated with deferoxamine B]. 用去铁胺B治疗受试者尿铁的半自动测定方法
Quaderni Sclavo di diagnostica clinica e di laboratorio Pub Date : 1988-01-01
R Grisler, C Giavardi, V Agape, R Scaricabarozzi, S Guercilena
{"title":"[Semiautomatic procedure for determining urine iron in subjects treated with deferoxamine B].","authors":"R Grisler,&nbsp;C Giavardi,&nbsp;V Agape,&nbsp;R Scaricabarozzi,&nbsp;S Guercilena","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A semiautomatic method for the determination of urine iron during treatment with deferoxamine B has been evaluated: samples were diluted 1:10 with a TCA 10% and thioglycolic acid 1.1% solution and processed after centrifugation by the aca III discrete clinical analyzer Du Pont. In this way an overestimation of values depending on the turbidity caused by proteins is avoided and the bad smell is limited thanks to the very low concentration of the thioglycolic acid solution. A good correlation with the atomic absorption spectrophotometry, specially for values less than or equal to 20 mg/l, was observed examining urine samples of 150 patients treated with deferoxamine B.</p>","PeriodicalId":76395,"journal":{"name":"Quaderni Sclavo di diagnostica clinica e di laboratorio","volume":"24 1-4","pages":"73-9"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14395148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[In vitro evaluation of the antibiotic effect of imipenen on bacterial strains isolated mostly from compromised patients]. [亚胺培南对主要来自感染患者的菌株的体外抗菌效果评价]。
Quaderni Sclavo di diagnostica clinica e di laboratorio Pub Date : 1988-01-01
M Bartolucci, F Galluzzi, G Sanchioni, G Aureli
{"title":"[In vitro evaluation of the antibiotic effect of imipenen on bacterial strains isolated mostly from compromised patients].","authors":"M Bartolucci,&nbsp;F Galluzzi,&nbsp;G Sanchioni,&nbsp;G Aureli","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The antibacterial activity in vitro of Imipenem (N-formimidoyl-thienamycin) was studied on 237 recently-isolated nosocomial bacterial strains and compared with Aztreonam, Cefotaxime, Ceftazidime, Ceftriaxone, Piperacillin, Amikacin and Netilmicin through the determination of susceptibility by the Kirby-Bauer method. Taking into account that 65% bacterial strains, out of the examined ones, came from antibiotic high-pressure departments (45% from bone-marrow transplanted patients in the Hematological Department and 20% from Intensive Care Unit patients), the results obtained in vitro show an activity of Imipenem on Gram-negative bacteria as much as a 92.7% susceptibility and a 75.7% on Gram-positive bacteria; when compared with the activity of the other antibiotics, Imipenem shows a really excellent activity.</p>","PeriodicalId":76395,"journal":{"name":"Quaderni Sclavo di diagnostica clinica e di laboratorio","volume":"24 1-4","pages":"203-12"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14395141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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