{"title":"DNA nuclear content of apocrine cells in fibrocystic mastopathy and breast cancer.","authors":"R Vasiu, C D Olinici","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The DNA nuclear content of apocrine cells was studied in 5 cases of fibrocystic disease, 3 cases of invasive duct carcinoma and 3 cases of invasive lobular carcinoma. Both diploid and aneuploid values were found in each group. The possible significance of apocrine change in breast lesions is discussed.</p>","PeriodicalId":76191,"journal":{"name":"Morphologie et embryologie","volume":"36 1","pages":"43-7"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13303456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D Laky, V Cândea, A Popa, I Tintoiu, B T Cândea, S Socolovschi
{"title":"Contributions to the biology of the hypoxic liver. Note II. Histologic, electron microscopic and biochemical aspects in the course of open heart surgery under extracorporeal circulation.","authors":"D Laky, V Cândea, A Popa, I Tintoiu, B T Cândea, S Socolovschi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A study was carried out on the evolution of histological and ultrastructural lesions of liver fragments harvested at different time intervals in the course of extracorporeal circulation in 62 patients operated for acquired and congenital heart disease, as well as that of serologic tests, pre-, intra- and postoperatively up to seven days. Morphologically, it is only the ultrastructural examination that detects the accentuation of preexisting hypoxic lesions within the framework of a state of \"controlled shock\", noting especially accentuated dilation of the endoplasmic reticulum, lysosomal activation, mitochondrial lesions and a tendency to ribosomal and glycogenic depletion. The lesions did not exceed the limits of reversibility, excepting the cases with advanced heart failure and cardiac cirrhosis. Lending support to these data is the decrease of proteinemia and the dynamics of LDH, SDH, G1DH, gamma GT and transaminases increase after 24 h, then fall to normal values within seven days.</p>","PeriodicalId":76191,"journal":{"name":"Morphologie et embryologie","volume":"36 1","pages":"19-23"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13303451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Gastric Burkitt's lymphoma. Case report and review of the literature.","authors":"C D Olinici, R Vasiu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A case of gastric Burkitt's lymphoma with pancreatic infiltration in a 15-year-old boy is reported. Local lymph nodes were not involved. Review of the literature disclosed that the reported cases of Burkitt's lymphoma were localized in the intestine. The origin, evolution and prognosis of gastrointestinal lymphoma are briefly discussed.</p>","PeriodicalId":76191,"journal":{"name":"Morphologie et embryologie","volume":"36 1","pages":"39-41"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13303455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The ultrastructure of the abdominal lymph nodes cortex in rats during primary and secondary immune response (a special reference to dendritic reticulum cells and interdigitating cells).","authors":"E Mandache, A Negoescu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The stimulation of coeliac rat lymph nodes was performed by intraperitoneal injections of typhoid vaccine and was unique for the primary immune response and repeated after 6 weeks for the secondary response. The light and electron microscopic observations showed for the primary response, an early germinal center reaction, which might be accounted for by a background of continuous stimulation of the coeliac nodes, stemming from the digestive tract. The dendritic reticulum cells (DRC), considered typical for the B area, were located at the borderline between the germinal center and the mantle zone. Their cytoplasmic extensions penetrated the lymphocyte-lymphoblastic center, surrounding most of the germinal center cells. The marginal zone and the paracortex reacted as a whole, the interdigitating cells (IDC) being the dominant feature. An explanation would be that the marginal zone can be penetrated by T cells and connected IDCs, thus, the B and T areas seem to be largely interspersed. The results suggest that IDCs are cells of direct monocytic origin.</p>","PeriodicalId":76191,"journal":{"name":"Morphologie et embryologie","volume":"36 1","pages":"49-58"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13303457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z Gârban, S Sandor, G Daranyi, A Prunkl, V Precob, I Gergen, I Eremia
{"title":"Homeostasis changes induced by the action of ethanol on the materno-fetal complex in rats. V. Late fetal effects of acute intoxication during the preimplantation period.","authors":"Z Gârban, S Sandor, G Daranyi, A Prunkl, V Precob, I Gergen, I Eremia","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The late fetal effect of ethanol administered during the preimplantation period (in acute experiment) was investigated. Ethanol was injected i.v. (33.16% v/v in a.d., 4.80 ml/kg b.w.) to pregnant female rats on day 2 and 4 of pregnancy. Some effects concerning biochemical and morphological homeostasis on at-term fetuses (day 20 of pregnancy) were studied. Data obtained were compared with those of the control group. Biochemical investigation performed on hepatic DNA and on some serum metabolites revealed the following statistically non-significant changes: the increase of fetal hepatic DNA; the increase of total protein determined from pooled fetal serum of the whole litters; hypoalbuminemy and hyperglobulinemy; hypo-alpha 1-globulinemy and hyper-alpha 2-, beta-, gamma-globulinemy; the increase of total lipids, decrease of cholesterol; increase of uric acid and urea. In the amniotic fluid the following statistically non-significant values were found: increase of proteins, lipids, uric acid and urea content and decrease of cholesterol. Ponderal somatometry evidenced a statistically significant decrease of fetal and placental wet weight. The changes found show that--in our experimental conditions--the i.v. administration of ethanol during the preimplantation period does not significantly influence the late, fetal biochemical values and induces a significant lowering of fetal and placental wet weight and a significant increase of late fetal mortality.</p>","PeriodicalId":76191,"journal":{"name":"Morphologie et embryologie","volume":"36 1","pages":"59-68"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13303458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Functional morphology of the bronchovascular barrier of the human lungs during various age periods.","authors":"V V Tomson, A G Türin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cellular components of the bronchovascular barrier have been studied in human lungs obtained after death of some patients with acute and chronic lung inflammatory diseases, hypertonic disease, atherosclerosis and chronic glomerulonephritis. Certain oxidative-reductive and hydrolytic enzymes, including NAD-, NADP- diaphorases, lactic dehydrogenase, acid and alkaline monophosphoesterase, ATP-ase, adenylate cyclase and nonspecific esterase were evaluated quantitatively after the histochemical processing of the specimens for the above reactions. Correlation analysis was performed for the bronchial epithelium, endotheliocytes, lymphocytes, plasma and mast cells, as well as macrophages and polymorphonuclear leucocytes. The results showed that there was a significant shift in some of the measured enzymic activities. Moreover, the correlations between different quantitative data were noted and these correlations changed with age. The increase in \"rigidity\" of the correlations in the elements of the bronchovascular barrier has been demonstrated during the process of ageing.</p>","PeriodicalId":76191,"journal":{"name":"Morphologie et embryologie","volume":"36 1","pages":"33-8"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13303454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The effect of human alpha leukocyte interferon (IFN) on the frequency of sister chromatid exchanges (SCE) in the KB cell line.","authors":"L Georgian, G Ghyka, I Savi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Concentrations of 50, 500 and 5,000 iu/ml of natural human alpha leukocyte interferon (IFN) were added into the culture medium of KB cells, 4 h after serial passage, in the presence of 5-bromo-desoxyuridine (Brdu) at 10 micrograms/ml. Similar cultures without IFN were set up as controls. After 72 h of incubation, the harlequin technique (differential staining of sister chromatids) was applied in order to discriminate among the metaphases of different generations and to appreciate the frequency of sister chromatid exchanges (SCE) in the second generation cells. The incidence of sister chromatid exchanges was slightly increased following IFN treatment but no dose-effect relationships were observed. At the same time, cell cycle kinetics estimated as replication index (RI) and average generation time (AGT) was not modified in IFN-treated cells as against the controls.</p>","PeriodicalId":76191,"journal":{"name":"Morphologie et embryologie","volume":"36 1","pages":"13-7"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13303450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bone marrow biopsy (BMB). III. Bone marrow biopsy in Hodgkin's disease (HD).","authors":"I Macavei, N Galatâr","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Bone marrow biopsies have been investigated in 330 cases of Hodgkin's disease totalising 298 patients, out of which 32 with repeated biopsies. Positive biopsies with typical lesions were found in 32% of patients, the majority in stages III and IV (88.6%), rarely in stage I or II (11.4%). Nonspecific lesions were very frequent (75%), either isolated or accompanied by typical lesions. The majority of the positive biopsies were found in patients with lymphocytic predominance and lymphocytic depletion, or in polytreated patients in an advanced stage of the disease. The specific marrow involvement consisted in lymphocytic infiltrations either nodular or diffuse, Reed-Sternberg (R-St) or Hodgkin cells. The lymphocytic depletion is often accompanied by diffuse fibrosis, atypical histiocytes, fibroblasts and R-St cells. Hodgkin typical granulomas are rare. The positive biopsies were associated with nonspecific reactions including hyperplasia of granulopoiesis, megakaryocytes, territories with hyperplasia or aplasia, fibrosis, disruption of sinus walls, oedema, plasmocytosis, necrosis, myelomonoblastic cells, lymphocyte nodes, etc. The bone marrow histology has a prognostic significance.</p>","PeriodicalId":76191,"journal":{"name":"Morphologie et embryologie","volume":"36 1","pages":"25-32"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13303452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Aspects of the thyroid gland vascularization in young organisms (a morphological and radioisotope-based study).","authors":"N Rottenberg, F Rottenberg, P Matusz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The thyroid gland vascularization has been studied on 58 pieces belonging to young organisms (human and animal) by means of both morphometric methods (macro- and microscopic) and injection of radioisotopes (86Rb). The statistical-mathematical study of the dimensional values characterizing the thyroid vascular branch at different levels assumed for study allowed to determine the thyroid central area as the best vascularized of the thyroid parenchyma. Estimating the specific activity and the absorption constant value of the 86Rb in the vascular wall and the glandular parenchyma, the radioisotope-based study attests the data obtained morphologically. By the simultaneous injection of 86Rb into strychnine, the pharmacodynamic study shows a more prominent reduction of the 86Rb absorption constant value in the distal area of the gland as compared to its central one and the blank batch.</p>","PeriodicalId":76191,"journal":{"name":"Morphologie et embryologie","volume":"36 1","pages":"3-8"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13303453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Histologic observations on the blood vessels of the fish brain.","authors":"T P Pessacq","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76191,"journal":{"name":"Morphologie et embryologie","volume":"36 1","pages":"9-11"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13304055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}