Alexandria Journal of Medicine最新文献_第9页

Clinical and epidemiological significance of RT-PCR and non-structural glycoprotein-1 assays in the diagnosis of dengue virus infections RT-PCR和非结构糖蛋白-1检测在登革热病毒感染诊断中的临床和流行病学意义

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Alexandria Journal of Medicine Pub Date : 2020-01-01 DOI: 10.1080/20905068.2019.1700334

I. Abdullahi, A. Ahmad, N. Faruku, B. Oderinde, A. Emeribe

{"title":"Clinical and epidemiological significance of RT-PCR and non-structural glycoprotein-1 assays in the diagnosis of dengue virus infections","authors":"I. Abdullahi, A. Ahmad, N. Faruku, B. Oderinde, A. Emeribe","doi":"10.1080/20905068.2019.1700334","DOIUrl":"https://doi.org/10.1080/20905068.2019.1700334","url":null,"abstract":"ABSTRACT Background: Due to the rapid geographic expansion, dengue has attracted much global attention. Hence, many research outputs have emanated from clinical and epidemiological studies. However, most of these studies, especially those from low- and middle-income countries, heavily relied on enzyme-linked immunosorbent assay (ELISA). Objective: In view of this, we sought to comment and remind dengue researchers within virology, immunology, and epidemiology disciplines regarding the limitations of ELISA protocols in establishing diagnosis of dengue virus (DENV) infections. Subsequently, we provided an update on the current diagnostic algorithm for dengue. Method: Extensive literature search was done using special key words on “PubMed”, “Scopus”, “Web of Science” and “Hinari”. Suitable articles were selected and subjected to scrutiny for inclusion in this study. Result: It was discovered that over 90% of published articles from LMICs inferred about dengue mainly from available commercial serological kits, without further confirmation using more accurate, sensitive and specific protocols. In some instances (less than 5%), combination of either RNA positive and anti-DENV IgM or dengue NS1 and anti-DENV IGM were used to diagnose acute primary dengue; while presence anti-DENV IgG and DENV RNA were considered non-primary dengue. Conclusion: In view of the limitations of every protocol used for investigations of dengue virus infections, its necessary to utilize appropriate combination tests to differentiate primary from non-primary dengue in order to generate reliable clinical and epidemiological inferences.","PeriodicalId":7611,"journal":{"name":"Alexandria Journal of Medicine","volume":"56 1","pages":"1 - 3"},"PeriodicalIF":0.9,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/20905068.2019.1700334","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43766134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Detection of Helicobacter pylori oipA and dupA genes among dyspeptic patients with chronic gastritis 慢性胃炎消化不良患者幽门螺杆菌oipA和dupA基因的检测

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Alexandria Journal of Medicine Pub Date : 2020-01-01 DOI: 10.1080/20905068.2020.1780675

Marwa El-Sayed, N. Musa, M. Eltabbakh, D. Abdelhamid, S. M. Mostafa, M. Salah, H. Faheem, R. Hassan

{"title":"Detection of Helicobacter pylori oipA and dupA genes among dyspeptic patients with chronic gastritis","authors":"Marwa El-Sayed, N. Musa, M. Eltabbakh, D. Abdelhamid, S. M. Mostafa, M. Salah, H. Faheem, R. Hassan","doi":"10.1080/20905068.2020.1780675","DOIUrl":"https://doi.org/10.1080/20905068.2020.1780675","url":null,"abstract":"ABSTRACT Helicobacter pylori (H. pylori) is a microbe with wide genetic diversity that infects the stomach of most people in developing countries, leading to several clinical outcomes among different individuals such as gastritis, ulcers, or gastric cancer. Outer inflammatory protein A (oipA) and duodenal ulcer promoting (dupA) genes are among the possible virulence factors which determine the patient outcome. Aim To detect oipA and dupA genes of H. pylori among dyspeptic Egyptian patients, and to investigate their correlation with the varying degrees of the associated chronic gastritis. Methods The study enrolled 50 patients with dyspepsia, attending the Gastrointestinal Endoscopy unit of the Gastroenterology and Tropical Departments at Ain Shams University Hospital for upper gastrointestinal endoscopy, in the period between, June and, December 2019. Four antral gastric biopsies were taken from each patient for polymerase chain reaction assay to detect the virulence genes oipA, dupA, and cagA and for histopathological assessment. Results Forty patients were H. pylori positive by histopathology and PCR. cagA, oipA, and dupA were identified in 6 (15%), 13 (32.5%), 9 (22.5%) of biopsies, respectively. Both cagA and oipA genes were highly significantly associated with increasing the severity of gastritis. Only oipA virulence gene showed a highly significant association with gastroduodenitis. There was a highly significant moderate association between cagA and oipA genes. Conclusion oipA could be a virulence biomarker that serves a great value in predicting the progress of gastric mucosal damage in patients with chronic gastritis, and targeting antimicrobial therapy in those patients to prevent severe gastroduodenal diseases.","PeriodicalId":7611,"journal":{"name":"Alexandria Journal of Medicine","volume":"56 1","pages":"105 - 110"},"PeriodicalIF":0.9,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/20905068.2020.1780675","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49317173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Is fenofibrate the missing piece in COVID-19 management? 非诺贝特是COVID-19管理中缺失的一环吗?

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Alexandria Journal of Medicine Pub Date : 2020-01-01 DOI: 10.1080/20905068.2020.1785144

E. Mikhael

引用次数: 0

Exercise-induced bronchospasm and its associated factors among secondary school students in an urban community 城市社区中学生运动性支气管痉挛及其相关因素分析

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Alexandria Journal of Medicine Pub Date : 2020-01-01 DOI: 10.1080/20905068.2020.1848238

A. Odeyemi, A. Odeyemi, O. Kayode, S. Oseni, O. Oyedeji

{"title":"Exercise-induced bronchospasm and its associated factors among secondary school students in an urban community","authors":"A. Odeyemi, A. Odeyemi, O. Kayode, S. Oseni, O. Oyedeji","doi":"10.1080/20905068.2020.1848238","DOIUrl":"https://doi.org/10.1080/20905068.2020.1848238","url":null,"abstract":"ABSTRACT Introduction: Exercise-induced bronchospasm (EIB) is a transient narrowing of the airway that usually occurs shortly after exercise. It occurs commonly in people with asthma; however EIB has also been reported in individuals without clinical asthma. The study aimed to determine the prevalence and factors associated with EIB among secondary school students. Methods: It was a descriptive cross-sectional school- based study involving secondary school students aged between 10 and 17 years without previous history of asthma. Subjects undertook a six-minute running test, spirometry was done and the best of three FEV1 readings were obtained pre-exercise, at 5, 10, 15 and 30 minutes post exercise. The diagnosis of EIB was made when there was a decrease in FEV1 of ≥10% from baseline after exercise. Results: Of the 265participants studied, 34 (12.8%) had EIB at 5 minutes post exercise. EIB was significantly more in the 10–13 years age group (χ2 = 18.416, p = <0.001), there was no significant gender difference. The presence of allergic (vernal) conjunctivitis and rhinitis were both significantly associated with the development of EIB (χ2 = 13.574, p < 0.001 and 0.011 respectively). There was no significant association with previous history of wheeze and exposure to indoor air pollution such as cooking with biomass fuel and parental cigarette smoking. Conclusion: EIB exist among non-asthmatic school children, and it is more frequent in the early adolescent age-group. Students with allergic conjunctivitis and rhinitis are more likely to manifest exercise induced bronchospasm.","PeriodicalId":7611,"journal":{"name":"Alexandria Journal of Medicine","volume":"56 1","pages":"203 - 209"},"PeriodicalIF":0.9,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/20905068.2020.1848238","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41990162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial resistance pattern and molecular epidemiology of ESBL and MBL producing Acinetobacter baumannii isolated from hospitals in Minia, Egypt 埃及米尼亚医院分离的产ESBL和MBL的鲍曼不动杆菌的耐药性模式和分子流行病学

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Alexandria Journal of Medicine Pub Date : 2020-01-01 DOI: 10.1080/20905068.2019.1707350

R. A. Abd El-Baky, S. M. Farhan, R. Ibrahim, K. Mahran, H. Hetta

{"title":"Antimicrobial resistance pattern and molecular epidemiology of ESBL and MBL producing Acinetobacter baumannii isolated from hospitals in Minia, Egypt","authors":"R. A. Abd El-Baky, S. M. Farhan, R. Ibrahim, K. Mahran, H. Hetta","doi":"10.1080/20905068.2019.1707350","DOIUrl":"https://doi.org/10.1080/20905068.2019.1707350","url":null,"abstract":"ABSTRACT Introduction: Multidrug resistant (MDR) Acinetobacter baumanii (A. baumannii) strains have emerged as novel nosocomial pathogens threatening patients’ lives, especially in intensive-care units (ICUs). This study aims to determine the prevalence of carbapenemase genes and CTX-M-15 and the resistance pattern of carbapenemase producing isolates.Methods: A total of 530 clinical specimens were collected from patients suffering from different infections, antibiotic susceptibility test was performed using kirby-bauer disk diffusion method. ESβL production was detected phenotypically by double-disc synergy test (DDST). Carbapenemase production was tested by Modified Hodge Test (MHT). Then, these isolates were tested for MBL detection by disc potentiation test. Carbapenemase encoding genes (VIM, IMP, GIM and SPM, OXA-51, OXA-23 and OXA-143) and CTX-M-15 were tested by polymerase chain reaction (PCR).Results: Out of 530 samples, 20 bacterial isolates were identified as A. baumannii from different infectious cases, 35% of isolates were ESBL-producers. Eleven isolates were resistant to imipenem (4 isolates) and meropenem (7 isolates). All carbapenem resistant isolates were MHT positive. Nine (45%) isolates were confirmed as A. baumannii by OXA-51 (all were carbapenem resistant). Distribution of IMP, VIM, GIM and SPM, OXA-23, OXA-143 and CTX-M-15 by PCR were 55, 50, 50, 25, 35, 45 and 33% respectively.Conclusion: The high prevalence of resistance genes and the resistance pattern of the isolates indicate that the detection of ESBLs and MBLs phenotypically and genotypically with the study of the resistance pattern of the isolates is critically important for the surveillance of drug resistance in the hospital environment.","PeriodicalId":7611,"journal":{"name":"Alexandria Journal of Medicine","volume":"56 1","pages":"4 - 13"},"PeriodicalIF":0.9,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/20905068.2019.1707350","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46351769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

A view of the health services after COVID-19: an Egyptian perspective 从埃及的角度看COVID-19后的卫生服务

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Alexandria Journal of Medicine Pub Date : 2020-01-01 DOI: 10.1080/20905068.2020.1789391

M. I. Kamel

引用次数: 10

The risk of obstructive sleep apnea and its association with indices of general and abdominal obesity in a Nigerian family practice clinic: a cross-sectional study 尼日利亚家庭诊所阻塞性睡眠呼吸暂停的风险及其与一般和腹部肥胖指数的关联:一项横断面研究

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Alexandria Journal of Medicine Pub Date : 2020-01-01 DOI: 10.1080/20905068.2019.1711304

O. Awopeju, M. Fawale, S. olowookere, O. Salami, O. Adewole, G. Erhabor

{"title":"The risk of obstructive sleep apnea and its association with indices of general and abdominal obesity in a Nigerian family practice clinic: a cross-sectional study","authors":"O. Awopeju, M. Fawale, S. olowookere, O. Salami, O. Adewole, G. Erhabor","doi":"10.1080/20905068.2019.1711304","DOIUrl":"https://doi.org/10.1080/20905068.2019.1711304","url":null,"abstract":"ABSTRACT Introduction: Obstructive sleep apnea (OSA) is associated with considerable morbidity and mortality. This study assessed the prevalence of high risk of OSA and investigated which anthropometric measure best predicts the OSA risk among patients attending a family practice clinic in a tertiary hospital. Methods: We conducted a descriptive cross-sectional study of 362 consecutive patients (64% females; median age of 54 years). OSA risk was assessed by the Berlin Questionnaire and the patients were divided into two groups according to OSA risk: high and low risk. Anthropometric measurements were conducted as stated in the protocol established in the 3rd National Health and Nutrition Examination Survey. Results: Out of 362 participants, 84 [23.2% (95% CI 19.0%, 28.0%)] had high risk of OSA. Subjects with a high risk of OSA had significantly higher body mass index, waist circumference, hip circumference, and waist-to-height ratio (24.9 vs 23.8, p = 0.002; 89.0 vs 84.0, p < 0.001; 95.0 vs 91.0, p < 0.001; 0.56 vs 0.52, p < 0.001, respectively). Body mass index, waist circumference, hip circumference, and waist-to-height ratio performed similarly in predicting high risk of OSA with Area Under the Curve (AUC) of 0.661, 95% CI (0.592,0.730); 0.659, 95% CI (0.596,0.723); 0.668, 95% CI (0.604,0.733); 0.659 95% CI (0.592,0.725) respectively. The AUCs were similar when the analysis was restricted to those who were overweight. Conclusion: High risk of OSA is moderately prevalent in this population, with measures of central and abdominal adiposity equally predicting the risk.","PeriodicalId":7611,"journal":{"name":"Alexandria Journal of Medicine","volume":"56 1","pages":"14 - 20"},"PeriodicalIF":0.9,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/20905068.2019.1711304","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49237784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Correction 校正

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Alexandria Journal of Medicine Pub Date : 2020-01-01 DOI: 10.1080/20905068.2020.1826535

引用次数: 0

Identification of urinary proteomic profile of patients with chronic allograft nephropathy 慢性移植物肾病患者尿蛋白质组学特征的鉴定

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Alexandria Journal of Medicine Pub Date : 2020-01-01 DOI: 10.1080/20905068.2020.1749782

Amr K. Hussien, P. Moez, H. el-Wakil, Hayam A. Elagaan

{"title":"Identification of urinary proteomic profile of patients with chronic allograft nephropathy","authors":"Amr K. Hussien, P. Moez, H. el-Wakil, Hayam A. Elagaan","doi":"10.1080/20905068.2020.1749782","DOIUrl":"https://doi.org/10.1080/20905068.2020.1749782","url":null,"abstract":"ABSTRACT Background and objectives Chronic allograft nephropathy (CAN) is a major complication that occurs post-transplantation. At present, the diagnosis of CAN is based on renal biopsy. Therefore, there is an ultimate need to identify more specific and sensitive noninvasive methods for the early diagnosis of CAN. Recently, proteomic-based modalities have been developed to discover biomarkers of CAN. Methods Urine samples from 75 participants were collected. Participants were divided into three groups: Group I: 25 patients with CAN, Group II: 25 transplanted patients with stable renal functions, and Group III: 25 healthy control subjects matched for age and sex. Each group was divided into training set and test set. Specimens were purified with magnetic beads-based weak cation exchange chromatography and analyzed using MALDI-TOF MS. Results A Genetic Algorithm (GA) was used to set up the classification models. Five peaks represented the proteomic profile that differentiates between the CAN patients and the control group with sensitivity of 100%, specificity of 100%, recognition capability of 100%, and cross-validation of 91.7% and five peaks differentiate between the transplant patients with normal renal functions and the control groups with sensitivity of 96.8%, specificity of 95.5%, recognition capability of 98%, and cross-validation of 100%. Interpretation and conclusions We identified a pattern for CAN and transplant patients with normal renal functions by proteomic profiling using MALDI-TOF-MS and magnetic beads.","PeriodicalId":7611,"journal":{"name":"Alexandria Journal of Medicine","volume":"56 1","pages":"93 - 104"},"PeriodicalIF":0.9,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/20905068.2020.1749782","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49106004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The potential cytoprotective effect of Vitamin C and Vitamin E on monosodium glutamate-induced testicular toxicity in rats 维生素C和维生素E对谷氨酸钠致大鼠睾丸毒性的潜在细胞保护作用

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Alexandria Journal of Medicine Pub Date : 2020-01-01 DOI: 10.1080/20905068.2020.1804311

Safaa Mohamed El Kotb, D. El-ghazouly, Omnia Ameen

{"title":"The potential cytoprotective effect of Vitamin C and Vitamin E on monosodium glutamate-induced testicular toxicity in rats","authors":"Safaa Mohamed El Kotb, D. El-ghazouly, Omnia Ameen","doi":"10.1080/20905068.2020.1804311","DOIUrl":"https://doi.org/10.1080/20905068.2020.1804311","url":null,"abstract":"ABSTRACT Background Monosodium glutamate (MSG) has been recognized as flavor enhancer that adversely affects male reproductive systems. Objective The study was conducted to explore the conceivable protective effects of vitamin C and/or vitamin E on testicular toxicity induced by MSG in rats. Materials and Methods Thirty male Wistar albino rats were divided (six per group) into: control, MSG, MSG + Vitamin C, MSG + Vitamin E and MSG + Vitamin C + Vitamin E groups. The duration of the study was three weeks. Assessment of serum testosterone, leuteinizing hormone (LH), malondialdehyde (MDA), glutathione peroxidase (GPX), interleukin-10 (IL-10) ,and tumor necrosis factor (TNF-α) were done. Histopathological examination of the testes of the rats was performed using histological, histochemical (Periodic Acid Schiff reaction (PAS)), and immunohistochemical (Proliferating cell nuclear antigen (PCNA), androgen receptors (ARs), Caspase-3) techniques. Results MSG-group was associated with significant decrease in serum testosterone, LH, GPX, and IL-10 (P < 0.05) and significant increase in serum MDA and TNF-α (P < 0.05) when compared with control group. MSG-group revealed many histopathological changes in the testis including degeneration of the germinal epithelium, absence of sperms in the lumina of tubules, widened vacuolated interstitium, marked deposition of the collagen fibers, very strong PAS reaction and marked immunohistochemical changes. Administration of vitamin C or vitamin E significantly reduced these changes; however, the combination of vitamin C and vitamin E provided more potent protection against the toxic effect of MSG than using each vitamin alone. Also, there was insignificant difference (P > 0.05) between MSG +Vitamin C and MSG +Vitamin E groups. Conclusion Vitamin C and Vitamin E act synergistically in reducing MSG-induced testicular toxicity via antioxidant, anti-inflammatory and antiapoptotic effects of both vitamins.","PeriodicalId":7611,"journal":{"name":"Alexandria Journal of Medicine","volume":"56 1","pages":"134 - 147"},"PeriodicalIF":0.9,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/20905068.2020.1804311","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47321665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8