Al-Azhar Journal of Pharmaceutical Sciences最新文献_第5页

A NEW TRIAL FOR BIOFORMATION OF ANTIMICROBIAL AGENT CONTROLLING MULTI DRUG RESISTANT MICROORGANISM 控制多重耐药微生物的抗菌药物生物合成新试验

Al-Azhar Journal of Pharmaceutical Sciences Pub Date : 2020-09-01 DOI: 10.21608/ajps.2020.118377

M. Elaasser

{"title":"A NEW TRIAL FOR BIOFORMATION OF ANTIMICROBIAL AGENT CONTROLLING MULTI DRUG RESISTANT MICROORGANISM","authors":"M. Elaasser","doi":"10.21608/ajps.2020.118377","DOIUrl":"https://doi.org/10.21608/ajps.2020.118377","url":null,"abstract":"In the present investigation, a trial was done to find a new antimicrobial agent producing microbe from soil microbiota of local habitats to control the problem of multiple drug resistance. The term of Antimicrobial resistance (AMR) is used to describe microorganisms that can resist the effects of drugs and chemicals designed to kill them. Seventy six actinomycetes isolates were isolated from fifteen soil samples different localities in Egypt were primary screening for antimicrobial activity by agar plug diffusion method against test microorganisms. Sixteen isolates were selected for secondary screening in small scale submerged fermentation system and assayed against pathogenic tested microorganisms using agar well diffusion method. Among of these isolates tested, the isolate (S1SHA1) showed the highest antimicrobial activity against pathogenic test organisms. This isolate was identified as Streptomyces griseoplanus by morphological, physiological, biochemical characters and 16s rRNA gene sequence. Physical and nutritional factors affecting activity of antimicrobial agent were studied. The results showed that, optimum activity of antimicrobial agent achieved with pH 7, incubation temperature 28 C, for 7 days, at 150 rpm agitation, carbon and nitrogen source starch 1.5% and potassium nitrate 0.4%, as well as phosphorus 2 g/l and NaCl at concentration of 1 %. Antimicrobial agent from batch culture was subjected to extraction and purification processes using ethyle acetate and preparative TLC, respectively. Determination of minimum inhibitory concentrations (MIC) and Mode of action of antimicrobial agent produced by S. griseoplanus (S1SHA1) on the test microbial strains using Transmission Electron Microscopy (TEM). Cytotoxic studies showed that no cytotoxic effects were observed for the compound when tested even at high concentrations","PeriodicalId":7603,"journal":{"name":"Al-Azhar Journal of Pharmaceutical Sciences","volume":"04 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86110909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SCREENING OF BIOSURFACTANT PRODUCTION BY BACTERIAL STRAINS ISOLATED FROM {SUDHANSHU SHEKHAR, 2015 #18}OIL CONTAMINATED SITES NEAR GAS STATIONS IN EGYPT. 从埃及加油站附近的石油污染场所分离的菌株筛选生物表面活性剂的生产。

Al-Azhar Journal of Pharmaceutical Sciences Pub Date : 2020-03-01 DOI: 10.21608/ajps.2020.86023

E. El-Gebaly

{"title":"SCREENING OF BIOSURFACTANT PRODUCTION BY BACTERIAL STRAINS ISOLATED FROM {SUDHANSHU SHEKHAR, 2015 #18}OIL CONTAMINATED SITES NEAR GAS STATIONS IN EGYPT.","authors":"E. El-Gebaly","doi":"10.21608/ajps.2020.86023","DOIUrl":"https://doi.org/10.21608/ajps.2020.86023","url":null,"abstract":"Biosurfactant producing bacteria (28 isolates) were isolated from 30 oil contaminated soils and tested for production of biosurfactant by different screening methods. About 75% of the isolated bacteria showed no blood hemolysis and their emulsification index for hexane and xylene ranges from 28-35% and 30-45% respectively. Biosurfactant producing ability was confirmed by other tests where about 40% of isolates showed positive oil spreading activity, hydrocarbon overlay and 67% showed to be positive for drop collapsing test. 16s RNA sequencing of the most active isolates revealed one Stenotrophomonas maltophila, two Bacillus spp. and two Achromobacter spp. Biosurfactants extracted from these isolates showed variable antimicrobial activity against Staphylococcus aureus, E. coli and Pseudomonas aeruginosa. This study demonstrated that the biosurfactants produced by these bacteria could be used in combination with antibiotics for treating bacterial infections. Further study is required for enhancing biosurfactant production by bacterial isolates to be used environmentally for bioremediation of oil contaminated soils.","PeriodicalId":7603,"journal":{"name":"Al-Azhar Journal of Pharmaceutical Sciences","volume":"109 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79211174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ANTIFUNGAL EFFECT OF GOLD NANOPARTICLES ON FUNGI ISOLATED FROM ONYCHOMYCOSIS PATIENTS 金纳米颗粒对甲真菌分离真菌的抑菌作用

Al-Azhar Journal of Pharmaceutical Sciences Pub Date : 2019-09-01 DOI: 10.21608/ajps.2019.70234

A. Sami

{"title":"ANTIFUNGAL EFFECT OF GOLD NANOPARTICLES ON FUNGI ISOLATED FROM ONYCHOMYCOSIS PATIENTS","authors":"A. Sami","doi":"10.21608/ajps.2019.70234","DOIUrl":"https://doi.org/10.21608/ajps.2019.70234","url":null,"abstract":"Onychomycosis is a widely distributed fungal nail infection which can be caused by dermatophytes, yeasts or non-dermatophytic fungi. In our study a total of 7 species related to 4 genera were isolated from 50 patients suspected with the fungal nail infection (onychomycosis) these namely Aspergillus flavus (8 isolates), Aspergillus niger (13 isolates), Candida albicans (8 isolates), C. tropicalis (5 isolates), Epidermophyton floccosum (1 isolate), Trichophyton mentagrophytes (2 isolates) and Trichophyton rubrum (1 isolate). Nowadays the field of nanotechnology becomes one of the most topics of interest. The impact of gold nanoparticles on the isolated fungal species was evaluated by agar well diffusion method and micro-dilution method. Best antifungal activity of gold nanoparticles was observed by using 100 µl of AuNps containing 20 µg of gold nanoparticles with the greatest zone of inhibition (19 mm) against C. albicans. AuNps showed variable MIC (minimum inhibitory concentration). MIC50 and MIC90 values of AuNPs ranged from 3.125 to 25.0 μg/ml and from 12.5 to100 μg/ml respectively. All isolated fungi could grow on keratin agar medium but with variable degrees indicating their ability to hydrolyze keratin. Keratinase activity in presence and absence of AuNPs was determined for dermatophytes and Aspergillus species. AuNPs had an inhibitory effect causing reduction in keratinase enzyme activity reaching 52.17 %, 40 % and 37.5 % was attained in case of E. floccosum, A. flavus and A. niger (the most susceptible isolates) respectively, by application of 20 µg/ml of AuNPs.","PeriodicalId":7603,"journal":{"name":"Al-Azhar Journal of Pharmaceutical Sciences","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84415240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

SYNTHESIS AND EVALUATION OF ANTIMICROBIAL ACTIVITY OF ARYLAZOLE DERIVATIVES 芳基唑衍生物的合成及抗菌活性评价

Al-Azhar Journal of Pharmaceutical Sciences Pub Date : 2019-09-01 DOI: 10.21608/AJPS.2019.70236

Ahmed Mancy

引用次数: 0

SYNTHESIS AND EVALUATION OF ANTIMICROBIAL ACTIVITY OF PHENYLPYRAZOLE DERIVATIVES 苯吡唑衍生物的合成及抗菌活性评价

Al-Azhar Journal of Pharmaceutical Sciences Pub Date : 2019-09-01 DOI: 10.21608/AJPS.2019.70238

Ali Hammad

引用次数: 0

APPLICATION OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR SIMULTANEOUS DETERMINATION OF RACECADOTRIL AND OFLOXACIN IN THEIR PHARMACEUTICAL DOSAGE FORM 高效液相色谱法同时测定药物剂型中消旋卡多曲和氧氟沙星的含量

Al-Azhar Journal of Pharmaceutical Sciences Pub Date : 2019-09-01 DOI: 10.21608/ajps.2019.70243

M. Kamel

引用次数: 2

LONG NON-CODING RNA H19 AS POTENTIAL BIOMARKER FOR HCV GENOTYPE 4 INDUCED HEPATOCELLULAR CARCINOMA PATIENTS 长链非编码RNA h19作为HCV基因型4诱导的肝细胞癌患者的潜在生物标志物

Al-Azhar Journal of Pharmaceutical Sciences Pub Date : 2019-09-01 DOI: 10.21608/AJPS.2019.70241

F. Fawzy

{"title":"LONG NON-CODING RNA H19 AS POTENTIAL BIOMARKER FOR HCV GENOTYPE 4 INDUCED HEPATOCELLULAR CARCINOMA PATIENTS","authors":"F. Fawzy","doi":"10.21608/AJPS.2019.70241","DOIUrl":"https://doi.org/10.21608/AJPS.2019.70241","url":null,"abstract":"The H19 gene is an oncofetal RNA expressed during embryo development and in several types of cancer. However, little is known about the role of the plasma H19 in liver cancer diagnosis. \u0000The current study aimed at measuring the plasma levels of long non-coding RNAs (H19) expression in chronic liver disease (CLD) due to HCV genotype 4 infections with/without cirrhosis and Hepatocellular carcinoma (HCC) patients in an attempt to evaluate the potential benefits of these new circulating, noninvasive, diagnostic, prognostic and epigenetic markers for liver cirrhosis and carcinogenesis of Egyptian patients. \u0000A hundred subjects were included in this study, divided into two groups; Group I (50 patients) were classified into subgroup Ia (CLD without cirrhosis, n=25) and subgroup Ib (CLD with cirrhosis, n=25), Group II (CLD patients with HCC, n=25), and control (Healthy volunteer, n=25). The expression of lncRNAs (H19) genes was analyzed by Real-Time PCR. \u0000LncRNAs (H19) showed upregulation in all diseased groups, which was in consistence with the progression of the disease toward the HCC stage. In addition, H19 showed a diagnostic ability to discriminate between cases of cirrhosis and HCC compared to healthy control (p< 0.001), while it did not show a discrimination significance differences between cirrhotic cases and non cirrhotic cases. By using receiver operating characteristic curve (ROC) analysis, it was found that H19 could diagnose HCC with AUC (81.4%). The increased H19 expression was associated with advanced tumor stages and higher grades. \u0000Plasma level of H19 marker might serve as a potential non-invasive biomarker for diagnosis of HCC.","PeriodicalId":7603,"journal":{"name":"Al-Azhar Journal of Pharmaceutical Sciences","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81092788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

CHEMERIN IS AN INDISPENSABLE PRE-TREATMENT PREDICTOR OF SOFOSBUVIR, PEGYLATED INTERFERON-ALPHA AND RIBAVIRIN OUTCOMES IN CHRONIC HEPATITIS C EGYPTIAN PATIENTS 在埃及慢性丙型肝炎患者中，Chemerin是索非布韦、聚乙二醇化干扰素和利巴韦林预后不可缺少的治疗前预测因子

Al-Azhar Journal of Pharmaceutical Sciences Pub Date : 2019-09-01 DOI: 10.21608/ajps.2019.70245

M. El-Derany

{"title":"CHEMERIN IS AN INDISPENSABLE PRE-TREATMENT PREDICTOR OF SOFOSBUVIR, PEGYLATED INTERFERON-ALPHA AND RIBAVIRIN OUTCOMES IN CHRONIC HEPATITIS C EGYPTIAN PATIENTS","authors":"M. El-Derany","doi":"10.21608/ajps.2019.70245","DOIUrl":"https://doi.org/10.21608/ajps.2019.70245","url":null,"abstract":"Chronic hepatitis C (CHC) treatment modalities dramatically evolve, however some patients still suffer treatment failure with sever aggressive side effects. Accordingly personalizing antiviral treatment becomes an urgent cornerstone in treatment path. Inflammation is one of the hallmarks of viral infection. Besides it is one of the main factors interfering with drug response. Accordingly, this study focused on studying some inflammatory markers interfering with antiviral response in different CHC patients. A cohort of 157 genotype 4 (G4) Egyptian CHC patients treated with sofosbuvir (SOF), pegylated interferon-alpha-2a (Peg-IFNα-2a) plus ribavirin (RBV) were enrolled. Single nucleotide polymorphism (SNP) within chemerin gene (rs17173608) was analyzed by quantitative real-time PCR (qRT-PCR). Serum chemerin, serum IL-6 and serum alpha-fetoprotein (AFP) were analyzed by enzyme-linked immunosorbent assay (ELISA). Fibrosis grade, serum AFP, serum IL-6 and serum chemerin levels were found to be significant univariant predictors of SOF, Peg- IFNα-2a and RBV response at (p<0.01). Moreover, G/G Chemerin (rs17173608) genotype was also proved to be associated with treatment failure (OR=17.067- 95% CI =6.684-43.575- p<0.001) in Egyptian CHC G4 patients. Combining all predictors in Multiple stepwise regression analysis concluded that higher serum chemerin levels and G/G Chemerin rs17173608 genotype formulated the strongest predictive model with a significant prediction capacity expressed by area under the receiving operating curve (AUROC) 0.881(95% CI = 0.832–0.931) with specificity of 77.1%, sensitivity of 80.2% and accuracy of 78.9%. Chemerin is a vital pretreatment predictor of anti-HCV drug response as it accurately predicts SOF, Peg- IFNα-2a and RBV different outcomes in naive Egyptian CHC G4 patients.","PeriodicalId":7603,"journal":{"name":"Al-Azhar Journal of Pharmaceutical Sciences","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85579243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

SYNTHESIS AND EVALUATION OF NEW PHENYLTHIAZOLE DERVATIVES AS ANTIMICROBIAL AGENTS 新型苯噻唑类抗菌药物的合成与评价

Al-Azhar Journal of Pharmaceutical Sciences Pub Date : 2019-09-01 DOI: 10.21608/AJPS.2019.70240

Hany G. Ezzat

{"title":"SYNTHESIS AND EVALUATION OF NEW PHENYLTHIAZOLE DERVATIVES AS ANTIMICROBIAL AGENTS","authors":"Hany G. Ezzat","doi":"10.21608/AJPS.2019.70240","DOIUrl":"https://doi.org/10.21608/AJPS.2019.70240","url":null,"abstract":"The current arsenal of antimicrobials couldn't hold back the rapid and ferocious growth of the antimicrobial resistance phenomenon. Collectively the current situation points to the pressing need to develop other effective antimicrobial agents. \u0000In the search for novel antimicrobials, phenylthiazole derivatives have recently been found to possess antibacterial activity particularly against a range of several antibiotic-resistant staphylococcus aureus strains. Modification of the nitrogenous head by incorporation the hydrolysable -HC=N- linkage within a pyrimidine ring bearing one or more hydrogen bond-promoting group led to the discovery of the second generation of phenylthiazoles, with more potent activity and longer half-lives compared to the lead compound 1a. \u0000Based on the above rationale, the objective of this study was to create a new set of structures of n-butylphenylthiazole-5-pyrimidine that contains two or more heteroatoms in their side chain and to study the relationship between the structural form of these compounds and their antimicrobial activity and spectrum against certain multi-drug resistance (MDR) strains. The new set of derivatives is designed to cover all the possibilities of side chains carbon-units distance and spatial configurations.","PeriodicalId":7603,"journal":{"name":"Al-Azhar Journal of Pharmaceutical Sciences","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91277844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ROLE OF BOSWELLIC ACID IN THE TREATMENT OF PEPTIC ULCER DISEASE 乳香酸在消化性溃疡疾病治疗中的作用

Al-Azhar Journal of Pharmaceutical Sciences Pub Date : 2019-09-01 DOI: 10.21608/ajps.2019.70246

M. Sherif

引用次数: 1