Al-Azhar Journal of Pharmaceutical Sciences最新文献

{"title":"SIMULTANEOUS DETERMINATION OF LEVAMISOLE AND OXYCLOZANIDE IN THE PHARMACEUTICAL PREPARATION BY CAPILLARY ELECTROPHORESIS","authors":"A. Ahmed, M. Nassar, A. El-Olemy, Mohamed S. Emara","doi":"10.21608/ajps.2022.269245","DOIUrl":"https://doi.org/10.21608/ajps.2022.269245","url":null,"abstract":"A sensitive, precise and accurate capillary electrophoresis method was developed for simultaneous determination of levamisole and oxyclozanide in pure sample and pharmaceutical preparation. Capillary electrophoresis was presented as a simple separation analytical method for the simultaneous analysis of the deliberated drugs within a shorter analytical run time. In this study, separation was achieved on fused silica capillary (30 cm - 50 µm internal diameter); background electrolyte solution consisted of phosphate buffer (40 mM, pH 7.9) and UV detection at 227 nm. The method showed to be linear (r 2 > 0.9998), precise (RSD <0.193%), accurate (recovery of 99.95% for levamisole and 100.12% for oxyclozanide), specific and robust. LOD and LOQ values were 0.099 µg mL -1 and 0.299 µg mL -1 respectively for levamisole and 0.075 µg mL -1 and 0.228 µg mL -1 respectively for oxyclozanide. The proposed method obtained well separation and had a perfect accuracy. The method was validated according to ICH guidelines and carried out for determination of the cited drugs in their pharmaceutical preparation.","PeriodicalId":7603,"journal":{"name":"Al-Azhar Journal of Pharmaceutical Sciences","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84385003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MOLECULAR CHARACTERIZATION OF KEY VIRULENCE TRAITS AMONG MULTIDRUG-RESISTANT METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS ISOLATES FROM SOME EGYPTIAN HOSPITALS","authors":"Abdel-Aty E Alfeky, M. Tawfick, M. Ashour, A. El-Moghazy","doi":"10.21608/ajps.2022.269250","DOIUrl":"https://doi.org/10.21608/ajps.2022.269250","url":null,"abstract":"Methicillin-resistant Staphylococcus aureus (MRSA) is an evolving cause of illness and death worldwide. MRSA strains can express a wide range of virulence factors that are implicated in their pathogenicity. The present study aimed to investigate the prevalence of crucial virulence traits encoding genes among MRSA isolates from Egyptian hospitals. A total of 170 S. aureus isolates were identified in this study from two Egyptian hospitals. These isolates were recovered from different clinical samples, during the period from September 2017 to December 2018. Of the 170 isolates, 138 (81.2%) were identified as MRSA by conventional microbiological methods and the identification was confirmed by the detection of methicillin resistance encoding gene mecA . Antimicrobial susceptibility was determined for MRSA isolates using the Kirby-Bauer disk diffusion method against 16 different antimicrobial agents representing diverse antimicrobial classes. Out of 109/138 (79%) Multidrug-resistant (MDR)-MRSA, fifty MDR-MRSA isolates were selected for further analysis of virulence encoding genes. MRSA isolates were resistant to different classes of antimicrobial agents including ꞵ-lactams, aminoglycosides, tetracyclines, macrolides and lincosamides. The antimicrobial resistance patterns among the selected 50 MDR-MRSA isolates revealed that the highest resistance rate was 100% to each of cefoxitin and penicillin, followed by doxycycline (80%), tetracycline (76%), gentamicin (74%), erythromycin (68%), clindamycin (60%) and azithromycin (50%). While the highest susceptibility rate was 88% to linezolid, followed by teicoplanin (66%), and amikacin (60%). Among the selected MDR-MRSA isolates, 52% were strong biofilm producers and 48% were moderate biofilm producers. The 50 MDR-MRSA isolates were screened for the presence of the virulence genes ( icaA , icaD , cna , hla , geh , tsst-1 and LukE/D ) that are implicated in their pathogenicity. The highest frequency of virulence genes in the selected MDR-MRSA isolates was 100% to each of icaD and geh , followed by hla (98%), icaA (96%), cna (92%), LukE/D (68%), and tsst-1 (56%). This study indicates that MRSA infection remains a significant problem in hospitals in Egypt. In addition, this study has verified a high prevalence of virulence factors among MRSA isolates from diverse clinical sources. Therefore, future studies on MRSA should aim to elucidate MRSA epidemiology, study antimicrobial susceptibility profiles, and investigate their virulence factors for effective control measures and better health management.","PeriodicalId":7603,"journal":{"name":"Al-Azhar Journal of Pharmaceutical Sciences","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74809402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MORINGA OLEIFERA CRUDE EXTRACT AND THE REFERENCE M2-CHANNEL BLOCKER AMANTADINE AS ANTIVIRALS AGAINST THE EGYPTIAN H5N8 ISOLATE, SPECIFICALLY INFLUENZA A/CHICKEN/EGYPT/Q16684C/2019 (H5N8) VIRUS.","authors":"Ahmed R. Sofy, K. Elsharkawy, A. Mousa, M. Ali","doi":"10.21608/ajps.2022.269249","DOIUrl":"https://doi.org/10.21608/ajps.2022.269249","url":null,"abstract":"Despite that great effort is currently running in the direction of vaccine and drug development, limited number of therapeutics are currently approved against seasonal influenza virus. To limit the spread of the transmission of highly pathogenic influenza (HPAI) viruses including HPAI H5N8 to mammals, it is recommended to control them in their natural and intermediate reservoirs, namely domestic poultry. Herein we aim to test both Moringa oleifera extract against the reference M2-channel blocker amantadine as antivirals against the Egyptian H5N8 isolate, specifically influenza A/chicken/Egypt/Q16684C/2019 (H5N8) virus. Interestingly, beside its high safety on MDCK cells (CC 50 = 15.42 mg/ml), the extract shows a promising and potent antiviral activity (IC 50 = 0.665 µg/ml) against HPAI H5N8, while the 50% inhibitory concentration (IC 50 ) of the amantadine was 56.81 µM. Moringa oleifera extract has shown a very promising effect as an anti-influenza, which makes it worthy of further studies.","PeriodicalId":7603,"journal":{"name":"Al-Azhar Journal of Pharmaceutical Sciences","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80190533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AN OVERVIEW OF IMIDES AND THEIR ANALOGUES AS ANTICANCER AGENTS","authors":"M. El-Zahabi, Yahia Amin, Helmy M. Sakr, Omar M. El-Hady","doi":"10.21608/ajps.2022.268400","DOIUrl":"https://doi.org/10.21608/ajps.2022.268400","url":null,"abstract":"","PeriodicalId":7603,"journal":{"name":"Al-Azhar Journal of Pharmaceutical Sciences","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88863803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PHYTOCHEMICAL CONSTITUENTS AND GAS CHROMATOGRAPHY WITH MASS SPECTROSCOPY ANALYSIS OF EUPHORBIA HETEROPHYLLA'S AERIAL PARTS","authors":"Hossam M Fouda, O. Abdulkader, Abd-Elmonem Sharaf, Mohammed H. Elhaw","doi":"10.21608/ajps.2022.268382","DOIUrl":"https://doi.org/10.21608/ajps.2022.268382","url":null,"abstract":"","PeriodicalId":7603,"journal":{"name":"Al-Azhar Journal of Pharmaceutical Sciences","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77661321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DESIGN AND SYNTHESIS OF NOVEL TERREMIDE DERIVATIVES FOR PHARMACOLOGICAL EVALUATION","authors":"Mohamed M. Elsebaei, Ahmed Mancy, Abdelrahman S. Mayhoub, M. Ayman","doi":"10.21608/ajps.2022.268386","DOIUrl":"https://doi.org/10.21608/ajps.2022.268386","url":null,"abstract":"","PeriodicalId":7603,"journal":{"name":"Al-Azhar Journal of Pharmaceutical Sciences","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82797283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NOVEL APPLICATION OF SPIRULINA PLATENSIS EXTRACT AS AN ALTERNATIVE TO THE EXPENSIVE PLANT GROWTH REGULATORS ON CAPPARIS CARTILAGINEA (DECNE.)","authors":"Marwa E. Abd El-Sadek, E. Ahmed","doi":"10.21608/ajps.2022.268248","DOIUrl":"https://doi.org/10.21608/ajps.2022.268248","url":null,"abstract":"","PeriodicalId":7603,"journal":{"name":"Al-Azhar Journal of Pharmaceutical Sciences","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75042540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HYPOURICEMIC EFFECT OF ANTIDESMA BUNIUS (L.) LEAVES EXTRACT AND IDENTIFICATION OF FLAVONOID CONTENT","authors":"D. Elnaggar","doi":"10.21608/ajps.2022.223765","DOIUrl":"https://doi.org/10.21608/ajps.2022.223765","url":null,"abstract":"Phytochemical investigation of Antidesma bunius (L.) spreng leaf ethyl acetate extract led to the isolation and identification of amentoflavone 7-O-β-D-glucoside, kaempferol 3-O-β-D-glucoside (Astragalin) and quercetin 7-O-α-L-rhamnopyranosyl(1 ′′′ →6 ′′ )-O-β-D-glucopyranoside (Quercetin-7-O-rutinoside) isolated for the first time from A. bunius leaf together with amentoflavone which is previously reported in A.bunius leaf. Detailed phenolics profile in A. bunius leaves ethyl acetate extract using HPLC-DAD-ESIMS / MS analysis revealed a total of 12 metabolites belonging to flavonoids and phenolic acids. Xanthine oxidase inhibition and hypouricemic effect in vitro bioassays recommends for the potential use of A. bunius leaf extract for the treatment of gout.","PeriodicalId":7603,"journal":{"name":"Al-Azhar Journal of Pharmaceutical Sciences","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83209720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BIODEGRADATION OF METHYL RED BY ACINETOBACTER BAUMANNII SM01 AND KLEBSIELLA PNEUMONIAE SM27 AMONG OTHER EGYPTIAN ISOLATES","authors":"Moaz Al-Sedawy","doi":"10.21608/ajps.2022.223774","DOIUrl":"https://doi.org/10.21608/ajps.2022.223774","url":null,"abstract":"Azo dyes are xenobiotic pose a long-term effect on human life. The capability of Acinetobacter baumannii SM01 and Klebsiella pneumoniae SM27 isolated from petroleum oil contaminated soil sites in Egypt for degradation of methyl red (MR) was investigated. The physicochemical parameters which affect MR degradation were examined. The maximum degradation of MR by both isolates was attained when 1.0 % (w/v) of glucose, 0.1 % (w/v) of yeast extract and 1.0 mM of CaCl2 were added to the culture media, and incubated for 6.0 h at 35°C with the highest speed of shaking (150 rpm). The mechanism of degradation of MR by Acinetobacter baumannii SM01 and Klebsiella pneumoniae SM27 isolates was studied through analysis of MR degradation products by FT-IR and double beam UV-visible spectrophotometer. Their results confirmed that MR underwent azo reduction and further degradation. The enzymes responsible for biodegradation of MR by Acinetobacter baumannii SM01 isolate were investigated, in the cell free medium obtained after 6.0 h of incubation and a control. The results showed a significant increase in the activities of laccase, tyrosinase, azoreductase and NADH-DCIP reductase enzymes. The outcomes of phytotoxicity studies on seeds of Triticum aestivum illustrated that MR degradation metabolites produced by Acinetobacter baumannii SM01 inhibited the germination rate by only 10 % and decreased root and shoot length by 27.8 % and 23.9 % respectively correlated to the inhibition exhibited by the intact MR. Consequently, the high efficiency of MR degradation enables Acinetobacter baumannii SM01 to be a potential candidate for bioremediation of MR containing wastewater.","PeriodicalId":7603,"journal":{"name":"Al-Azhar Journal of Pharmaceutical Sciences","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73136094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PHYTOCHEMICAL AND BIOLOGICAL INVESTIGATION OF KOELREUTERIA ELEGANS (SEEM.) LEAVES","authors":"Dina El Naggar","doi":"10.21608/ajps.2022.223766","DOIUrl":"https://doi.org/10.21608/ajps.2022.223766","url":null,"abstract":"A new galloylquinic acid butyl ester; 1, 3, 4, 5-tetra-O-galloylquinic acid butyl ester (1) and other ten known phenolic compounds : 1,3,4,5-tetra-O-galloylquinic acid (2) , methyl gallate (3), gallic acid (4) , 3 \" -galloyl quercetrin (5), isorhamnetin-3-O-β-D4 C1-arabinopyranoside (6), isorhamnetin-3O-α-L1 C4-rhamnopyranoside (7), quercetrin (8), guaijaverin (9), quercetin (10), azaleatin (11) as well as, three triterpenoidal compounds oleanolic acid (12), oleanolic acid-3-O-β-D4 C1glucopyranoside (13), 3-O-[O-α-L-rhamnopyranosyl-(1-2)-O-β-D-glucopyranosyl-(12)β-D4 C1-glucopyranosyl] oleanolic acid (14) were isolated from the 70 % methanol extract of leaves of Koelreuteria elegans (Seem.). The structures of the isolated compounds were elucidated on the basis of chemical and spectroscopic analysis (NMR, -ESIMS and UV). Total phenolic and flavonoid contents were found to be 8.19 g of GAE (gallic acid equivalents) and 6.22 g of RE (rutin equivalents) per 100 g dry extract respectively. The extract significantly improved the main liver function enzymes (ALT, AST) and total bilirubin in addition to the significant hepatoprotective action against the depletion of hepatic GSH and serum vitamin C levels , as well as the changes of hepatic antioxidant enzymes levels (SOD, CAT) induced by CCl4 toxicity. It non-significantly reduced the level of ALP and GGT. Moreover, compound 1 exhibited higher activity than aqueous methanol extract against both Gram positive and Gram negative organisms and Geotricum candidum (fungi). Key world: Koelreuteria elegans; Flavonols; Triterpenoidal saponin; Hepatoprotective effect and Antimicrobial activity. Az. J. Pharm Sci. Vol. 65, March, 2022. 17","PeriodicalId":7603,"journal":{"name":"Al-Azhar Journal of Pharmaceutical Sciences","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79268392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}