Journal of clinical & laboratory immunology最新文献_第9页

Natural killer cell frequency and function in patients with monoclonal gammopathies. 单克隆伽玛病患者的自然杀伤细胞频率和功能。

Journal of clinical & laboratory immunology Pub Date : 1992-01-01

G Famularo, A D'Ambrosio, F Quintieri, S Di Giovanni, I Parzanese, F Pizzuto, R Giacomelli, O Pugliese, G Tonietti

引用次数: 0

Effects of cryopreservation on immune responses: VI. An inexpensive method for freezing human peripheral blood mononuclear cells. 低温保存对免疫反应的影响:六。一种廉价的冷冻人外周血单核细胞的方法。

Journal of clinical & laboratory immunology Pub Date : 1992-01-01

M Venkataraman

引用次数: 0

Herbal medicine "sho-saiko-to" induces in vitro granulocyte colony-stimulating factor production on peripheral blood mononuclear cells. 中药“shoo -saiko-to”在体外诱导外周血单核细胞产生粒细胞集落刺激因子。

Journal of clinical & laboratory immunology Pub Date : 1992-01-01

M Yamashiki, M Asakawa, Y Kayaba, Y Kosaka, A Nishimura

{"title":"Herbal medicine \"sho-saiko-to\" induces in vitro granulocyte colony-stimulating factor production on peripheral blood mononuclear cells.","authors":"M Yamashiki, M Asakawa, Y Kayaba, Y Kosaka, A Nishimura","doi":"","DOIUrl":"","url":null,"abstract":"The herbal medicine \"Sho-saiko-to (Xiao-Chai-Hu-Tang)\" has been used in China for about 3000 years for the treatment of pyretic diseases. This medicine is now available as one of the prescribing drugs approved by the Ministry of Health and Welfare of Japan, and has also been widely used for patients with chronic viral liver disease as one of biological response modifiers in the field of Japan's Western Medicine. However, its mode of action has not been fully described. In the present in vitro study, we added \"Sho-saiko-to\" (TJ-9, Tsumura, Tokyo) to the culture of peripheral blood mononuclear cells (PBMC) obtained from healthy volunteers, and observed a dose-dependent increase in the production of granulocyte colony-stimulating factor (G-CSF). The same experiment was conducted using other herbal medicines \"Dai-saiko-to\" (TJ-8) and \"Saiko-keishi-to\" (TJ-10) which showed similar effects, or \"Sho-seiryu-to\" (TJ-19) which consists of very different compounds and shows different efficacy. The increases of G-CSF production were similar when \"Sho-saiko-to\" (TJ-9) or one of the 2 reference drugs (TJ-8 and 10) was added, whereas the increase when the control drug \"Sho-seiryu-to\" (TJ-19) was added, was quite small. This result shows that G-CSF induction is not a common effect of herbal medicines, but a specific effect of TJ-8, 9, and 10. Among these 3 drugs the increase produced by \"Sho-saiko-to\" was the largest. Based on this result, we conclude that administration of \"Sho-saiko-to\" may be useful not only for the treatment of chronic liver disease, but also for malignant diseases and acute infectious diseases where G-CSF is efficacious.","PeriodicalId":75994,"journal":{"name":"Journal of clinical & laboratory immunology","volume":"37 2","pages":"83-90"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12459667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of topical exposure to chemical allergens on murine Langerhans cells. Comparison of 2,4-dinitrochlorobenzene with trimellitic anhydride. 局部暴露于化学过敏原对小鼠朗格汉斯细胞的影响。2,4-二硝基氯苯与三苯酸酐的比较。

Journal of clinical & laboratory immunology Pub Date : 1992-01-01

M Cumberbatch, S J Gould, S W Peters, D A Basketter, R J Dearman, I Kimber

{"title":"Influence of topical exposure to chemical allergens on murine Langerhans cells. Comparison of 2,4-dinitrochlorobenzene with trimellitic anhydride.","authors":"M Cumberbatch, S J Gould, S W Peters, D A Basketter, R J Dearman, I Kimber","doi":"","DOIUrl":"","url":null,"abstract":"Chemical allergens differ with respect to the type of hypersensitivity reactions they preferentially elicit. Some chemicals, such as trimellitic anhydride (TMA), have the potential to induce both contact and respiratory hypersensitivity. Other chemicals cause only contact allergy. For example, 2,4-dinitrochlorobenzene (DNCB), a potent contact allergen, appears not to induce respiratory sensitization. In previous studies we have shown that topical exposure of mice to TMA and DNCB, under conditions of equivalent immunogenicity with respect to draining lymph node activation and contact sensitization, caused qualitatively different antibody responses. While the chemicals provoked IgG anti-hapten antibody responses of equivalent magnitude, only TMA induced an IgE response, and DNCB caused a significantly stronger IgG2a response. These data are consistent with the preferential activation by DNCB and TMA of Th1 and Th2 cells respectively. The purpose of the present study was to examine whether the qualitative differences in immune responses stimulated by these chemicals is reflected by variable affects on Langerhans cells (LC) in situ. Mice were exposed to concentrations of DNCB (1%) and TMA (50%) which caused equivalent levels of contact sensitization. Under these conditions topical exposure to DNCB, but not to TMA, or to vehicle alone, resulted in increased expression by LC of Ia antigen. Similar treatment with an irritant concentration (20%) of sodium dodecyl sulphate failed to influence Ia expression by LC. These data indicate that, at concentrations which induce similar levels of skin sensitization, not all contact allergens cause rapid changes in LC Ia expression, and that the qualitative differences in immune responses elicited by chemical allergens DNCB and TMA is associated with variable effects on LC.","PeriodicalId":75994,"journal":{"name":"Journal of clinical & laboratory immunology","volume":"37 2","pages":"65-81"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12514021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronical administration of anti-DNA antibodies by subcutaneous injection of hybridoma clones in BALB/c and NZBxNZW/F1 mice. Experimental model of the pathogenic role of DNA in acute lupus disease. BALB/c和NZBxNZW/F1小鼠皮下注射杂交瘤克隆慢性抗dna抗体。DNA在急性狼疮疾病中致病作用的实验模型。

Journal of clinical & laboratory immunology Pub Date : 1992-01-01

M Gayral-Taminh, I Delobbe, M A Mignon-Conte, M Dueymes, J J Conte

引用次数: 0

Reduced CD4-CD8 T cell ratios in patients with Wegener's granulomatosis. 韦格纳肉芽肿患者CD4-CD8 T细胞比例降低。

Journal of clinical & laboratory immunology Pub Date : 1992-01-01

M Ikeda, S Tsuru, Y Watanabe, S Kitahara, T Inouye

引用次数: 0

Induction of monocytic cell adherence to matrix-bound fibronectin by phorbol ester. 磷酸酯诱导单核细胞粘附基质结合纤维连接蛋白。

Journal of clinical & laboratory immunology Pub Date : 1992-01-01

P Roth, R A Polin

引用次数: 0

Immunoglobulin-complexed aspartate aminotransferase with a possible association with ulcerative colitis and its activity. 免疫球蛋白复合天冬氨酸转氨酶与溃疡性结肠炎的可能关联及其活性。

Journal of clinical & laboratory immunology Pub Date : 1992-01-01

H Tajiri, T Nakano, K Kozaiwa, T Harada, S Okada, R Fushimi, N Amino, K Miyai

引用次数: 0

Selective detection of human hepatitis B virus surface and core antigens in some peripheral blood mononuclear cell subsets by flow cytometry. 流式细胞术选择性检测人乙型肝炎病毒表面抗原和部分外周血单核细胞亚群的核心抗原。

Journal of clinical & laboratory immunology Pub Date : 1992-01-01

I Chemin, C Vermot-Desroches, I Baginski, J C Saurin, F Laurent, F Zoulim, J Bernaud, J P Lamelin, O Hantz, D Rigal

{"title":"Selective detection of human hepatitis B virus surface and core antigens in some peripheral blood mononuclear cell subsets by flow cytometry.","authors":"I Chemin, C Vermot-Desroches, I Baginski, J C Saurin, F Laurent, F Zoulim, J Bernaud, J P Lamelin, O Hantz, D Rigal","doi":"","DOIUrl":"","url":null,"abstract":"The presence of HBs and HBc antigens was investigated, by flow cytometry, on the surface of peripheral mononuclear cells (PBMC) from the following phenotype: CD3 (T lymphocytes), CD4 (T helper/inducer), CD8 (T cytotoxic/suppressor), CD19 (B lymphocytes) and CD56 (NK cells) among 8 patients suffering from chronic hepatitis B and 5 healthy HBV-negative subjects. This study demonstrated the presence of HBsAg and HBcAg on the lymphocyte surface for most of the patients. The mean percentage of labelled cells was 17% for HBsAg and 15% for HBcAg. Among the different lymphocyte subsets only B lymphocytes and the NK cells expressed HBsAg for 57% and 26% of cells, respectively. Similarly HBcAg was also detected among CD19 and CD56 cells only. PCR was used to search for the presence of HBV DNA and RNA in PBMC, using primers located in the S gene. HBV DNA was detected with variable intensity in the CD3, CD4, CD19 and CD56 subsets following their separation with a cell sorter. For HBV RNA the signal obtained after PCR and Southern blotting was higher for CD56 and CD19 cells than for CD3 cells and undetectable for CD4 cells. This study demonstrates that replication and transcription can occur in CD19 and CD56 cells. Positive signals in CD3 cells may possibly be due to contamination of this subpopulation by NK cells.","PeriodicalId":75994,"journal":{"name":"Journal of clinical & laboratory immunology","volume":"38 2","pages":"63-71"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12517257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral polyvalent vaccine (Buccalin Berna) administration activates selected T-cell subsets and regulates the expression of polymorphonuclear leukocyte membrane molecules. 口服多价疫苗(Buccalin Berna)激活选定的t细胞亚群并调节多形核白细胞膜分子的表达。

Journal of clinical & laboratory immunology Pub Date : 1992-01-01

A Fattorossi, R Biselli, A Casciaro, V Trinchieri, C De Simone

{"title":"Oral polyvalent vaccine (Buccalin Berna) administration activates selected T-cell subsets and regulates the expression of polymorphonuclear leukocyte membrane molecules.","authors":"A Fattorossi, R Biselli, A Casciaro, V Trinchieri, C De Simone","doi":"","DOIUrl":"","url":null,"abstract":"Acute respiratory tract infections (ARIS) still represent a major clinical problem during influenza outbreaks. The virus-induced impairment of the immune system favors the entry of opportunistic microorganisms into respiratory tract mucosa. A useful strategy to reduce ARIS is to provide at risk subjects an orally administrable polyvalent vaccine (OPV) comprised of bacteria strains recognised as the major ones responsible for ARIS. For the present study, the main circulating leukocyte populations of a group of healthy subjects receiving OPV were monitored as a marker of immune response. Subjects were investigated immediately before taking OPV (day 0) and 10,20, and 30 days later. Data show that T lymphocytes were induced to enhance the expression of class II MHC molecules, whilst a consistent number of CD4+ lymphocytes lost L-selectin, both phenomena indicating an activation status. OPV administration also modulated important molecules on the membrane of polymorphonuclear leukocytes, namely CD11b and CD16, strongly suggesting an activation of these cells and an enhancement of their defensive capacities. Finally, OPV was found to be able to increase the titer of serum antibodies specific for bacteria strains contained in the vaccine preparation in a portion of individuals. We conclude that OPV is able to consistently influence important immune functions and suggest that this property may be of relevance in preventing ARIS. Also, the present data may help to further our understanding of the mechanisms of OPV activity.","PeriodicalId":75994,"journal":{"name":"Journal of clinical & laboratory immunology","volume":"38 2","pages":"95-101"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12517260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0