Jikken dobutsu. Experimental animals最新文献

{"title":"A 3-Mbp fragment on rat chromosome 1 affects susceptibility both to stroke and kidney injury under salt loading in the stroke-prone spontaneously hypertensive rat: a genetic approach using multiple congenic strains.","authors":"Mei Wang, Hiroki Ohara, Masahiro Egawa, Shohei Fukunaga, Hiroyuki Matsuo, Zhi-Ru Ge, Toru Nabika","doi":"10.1538/expanim.21-0189","DOIUrl":"10.1538/expanim.21-0189","url":null,"abstract":"<p><p>We have previously reported that a major quantitative trait locus (QTL) responsible for susceptibility to salt-induced stroke in the stroke-prone spontaneously hypertensive rat (SHRSP) is located in a 3-Mbp region on chromosome 1 covered by SHRSP.SHR-(D1Rat23-D1Rat213)/Izm (termed Pr1.31), a congenic strain with segments from SHRSP/Izm introduced into the stroke-resistant SHR/Izm. Here, we attempted to narrow down the candidate region on chromosome 1 further through analyses of subcongenic strains constructed for the target region. Simultaneously, salt-induced kidney injury was evaluated through the measurement of urinary albumin and the gene expression of renal tubular injury markers (Kim-1 and Clu) to explore a possible mechanism leading to the onset of stroke. All subcongenic strains examined in this study showed lower susceptibility to salt-induced stroke than SHRSP. Interestingly, Pr1.31 had the lowest stroke susceptibility when compared with newly constructed subcongenic strains harboring fragments of the congenic sequence in Pr1.31. Although Kim-1 and Clu expression after 1 week of salt loading in Pr1.31 did not differ significantly from those in SHRSP, the urinary albumin level of Pr1.31 was significantly lower than those of the other subcongenic strains and that of SHRSP. The present results indicated that, although the congenic fragment in Pr1.31 harbored the gene(s) related to salt-induced organ damages, further genetic dissection of the candidate region was difficult due to multiple QTLs suggested in this region. Further analysis using Pr1.31 will unveil genetic and pathophysiological mechanisms underlying salt-induced end organ damages in SHRSP.</p>","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"71 1","pages":"368-375"},"PeriodicalIF":0.0,"publicationDate":"2022-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9388333/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48249190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calcium carbonate supplementation causes motor dysfunction.","authors":"Ami Sugiura, Misa Kitamura, Yasushi Hasegawa","doi":"10.1538/expanim.22-0011","DOIUrl":"10.1538/expanim.22-0011","url":null,"abstract":"<p><p>We previously showed that a diet containing calcium carbonate causes impairments in spatial and recognition memory in mice. In this study, we investigated the effects of calcium carbonate supplementation on motor function. Motor function was determined using different tests that have been used to analyze different aspects of Parkinsonism. A catalepsy test for akinesia; a muscular strength assessment, pole test, beam-walking test, and gait analysis for motor coordination and balance assessment; and an open-field test for locomotor activity assessment were performed. The mice were fed diets containing 0.6% or 1.0% calcium carbonate for eight weeks, after which they were evaluated for motor functions. The diets containing calcium carbonate caused significant motor dysfunction, as revealed by the different tests, although the spontaneous locomotor activity did not change. Calcium carbonate supplementation decreased the dopamine content in the basal ganglia, including the striatum and substantia nigra, and the number of tyrosine hydroxylase-positive neurons in the substantia nigra. In addition, administration of L-dopa led to at least a partial recovery of motor dysfunction, suggesting that calcium carbonate supplementation causes motor dysfunction by decreasing the dopamine content in the basal ganglia. These results suggest that mice with calcium carbonate-induced motor dysfunction may be useful as a new animal model for Parkinson's disease and Huntington's disease.</p>","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"71 1","pages":"399-410"},"PeriodicalIF":0.0,"publicationDate":"2022-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9388334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47468083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LAS Seminar 3","authors":"","doi":"10.1538/expanim.71suppl-LAS3","DOIUrl":"https://doi.org/10.1538/expanim.71suppl-LAS3","url":null,"abstract":"","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"71 1","pages":"S64 - S64"},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47868142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Symposium 6","authors":"","doi":"10.1538/expanim.71suppl-S6","DOIUrl":"https://doi.org/10.1538/expanim.71suppl-S6","url":null,"abstract":"","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"71 1","pages":"S31 - S36"},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45076147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral Presentation (Best Presentation Award)","authors":"Shingo Kawano, J. Bai, G. Sashida, Imari Furukawa, K. Yoshinobu, Yuri Kitamoto, Satoru Takahashi, T. Kaname, K. Araki, M. Araki","doi":"10.1538/expanim.71suppl-OP","DOIUrl":"https://doi.org/10.1538/expanim.71suppl-OP","url":null,"abstract":"Sperm...have...a...characteristic...structure...called...the...“mitochondrial...sheath,”... in...which...mitochondria...are... tightly... coiled...around...the...flagellum....However,... the...molecular...mechanisms... involved... in...the... formation...of... this...structure... have...not...been...well... studied.... In... this... study,...we... investigated... the... function...of...ARMC12... that... is... specifically... expressed... in... the...testis... to...clarify... the...molecular...mechanisms... involved... in... the... formation...of... the...mitochondrial... sheath.... We...generated...several... testis-enriched...gene...KO...mice...using... the...CRISPR/Cas9...system...and...examined... their... fertility...and...sperm...morphology....Armc12...KO...male...mice...are...infertile,...and...KO...spermatozoa...showed...abnormal... morphology...and...reduced...motility....Scanning...electron...microscopy...revealed...that...sperm...mitochondria...in...KO...mice... failed... to...elongate...properly...around... the... flagellum....ARMC12... is... localized...on... the...outer...membrane...of... sperm... mitochondria...as...a...peripheral...membrane...protein.... Immunoprecipitation...revealed...that...ARMC12... interacts...with... several... proteins,... including...VDAC2... and...VDAC3,...which... are... channel... proteins... localized... on... the... outer... mitochondrial...membrane,...and...TBC1D21,...which...is...expressed...specifically...in...the...testis. Tbc1d21...KO...mice...showed...the...same...abnormalities...in...mitochondrial...sheath...formation...as...Armc12...KO...mice,...and... the...protein... interaction...between...ARMC12...and...VDAC...was...not...observed... in...Tbc1d21...KO...testis.... In...addition,... experiments...using...cultured...cells...showed...that...mitochondria... in...ARMC12-expressing...cells...aggregate,... indicating... that...ARMC12...acts...as...an...adherence...factor...between...mitochondria. These...results...suggest...that...ARMC12...acts...as...an...adherence...factor...between...mitochondria...by...cooperating...with... TBC1D21,...and...uses...VDAC...proteins...as...a...scaffold....This...suggests...that...ARMC12...enables...sperm...mitochondria...to... correctly...elongate...around...the...flagellum...during...spermatogenesis,...resulting...in...the...formation...of...a...finely...organized... mitochondrial...sheath. ARMC12 enables mitochondrial sheath formation in cooperation with TBC1D21","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"71 1","pages":"S65 - S76"},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43386416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Encouragement Award","authors":"","doi":"10.1538/expanim.71suppl-L2","DOIUrl":"https://doi.org/10.1538/expanim.71suppl-L2","url":null,"abstract":"","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"71 1","pages":"S2 - S3"},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42950827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ando-Tajima Award","authors":"","doi":"10.1538/expanim.71suppl-L1","DOIUrl":"https://doi.org/10.1538/expanim.71suppl-L1","url":null,"abstract":"","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"71 1","pages":"S1 - S1"},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47540386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Symposium 4","authors":"","doi":"10.1538/expanim.71suppl-S4","DOIUrl":"https://doi.org/10.1538/expanim.71suppl-S4","url":null,"abstract":"","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"71 1","pages":"S22 - S24"},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42003235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LAS Seminar 2","authors":"K. Mominoki","doi":"10.1538/expanim.71suppl-LAS2","DOIUrl":"https://doi.org/10.1538/expanim.71suppl-LAS2","url":null,"abstract":"The...Japanese...Association...of...Laboratory...Animal...Facilities...of...National...University...Corporations...(JALAN)...aims... to... improve... the...accuracy...and...standard...of...animal...experiments...at...national...university...corporations,... and... to... promote...the...refinement...of...care...and...use...for...laboratory...animals....JALAN...has...been...organized...about...50...years...ago... for...the...purpose...of...establishing...a...communication...system...and...cooperation...system...between...the...animal...facilities... in...order...to...contribute...to...the...promotion...of...education...and...research...in...the...life...science....Since...its...establishment,... the...JALAN...has...been...working...with...the...recognition...that...“people”...are...important...to...support...the...animal...facilities... and... the...scientific... fields... that... form...the...basis...of... their...activities.... In...particular,... in...1971-1995,...when... the... initial... membership... facilities...were...established... in... the...national...universities,... there...were...no... institutions... for... training... “people”...such...as...experimental...animal...technologists,...so...it...is...important...to...train...those....Therefore,...we...have...been... holding...workshops...for...experimental...animal...technologists...every...year...since...1995. By...the...way,... the...workshop... in...2020...was...planned...to...be...held... jointly...by...the...two...animal... facilities... in...Hokkaido... University.... In...January,...2020,... the...first...person... infected...with...COVID-19...was...confirmed... in...Japan,...and... in...April,... 2020,... an... “emergency...declaration”...based...on... the...Act... for...Pandemic... Influenza...and...New...Infection...Diseases... Preparedness...and...Response...was...nationally...issued....Although...the...declarations...of...emergency...were...lifted...by...May... 25,...2020,... the...workshop... in...2020...was...canceled...due...to... the...strong...demand... for... thorough...measures...to...prevent... infection....The... secretariat... of... JALAN...had...decided... to...produce... the... training...video... for... animal... experiment... technologists...instead...of...the...workshop...in...2020....The...produced...video...was...distributed...to...the...JALAN...members...at... the...JALAN...on-lined...meeting...in...2021....At...this...LAS...seminar,...I...had...the...opportunity...to...introduce...this...vide,...so...I... will...explain...the...background...and... intention...of... the...video...production,...and...the...way...of...thinking...when...using...this... video. Production of Education and Training Video -Basic Techniques for Rabbits, Dogs, and Pigs Uses-","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"71 1","pages":"S56 - S63"},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42584455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Symposium 3","authors":"","doi":"10.1538/expanim.71suppl-S3","DOIUrl":"https://doi.org/10.1538/expanim.71suppl-S3","url":null,"abstract":"","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"71 1","pages":"S19 - S21"},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42612480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}