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Folia histochemica et cytochemica最新文献

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Changes in the cell surface architecture in normal and polyoma virus transformed hamster cells after infection with influenza virus. 感染流感病毒后正常和多瘤病毒转化的仓鼠细胞表面结构的变化。
Folia histochemica et cytochemica Pub Date : 1980-01-01
T Ochałek
{"title":"Changes in the cell surface architecture in normal and polyoma virus transformed hamster cells after infection with influenza virus.","authors":"T Ochałek","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Test of Con A induced cell agglutination, method of binding cells to Con A coated nylon fibres and modified procedure of cell-to-cell binding were used in the investigation of architectural surface changes in normal and polyoma virus transformed hamster cells infected with influenza virus. In both cell types influenza virus infection caused 1) increase in fixation resistant Con A agglutination, 2) decrease in the level of surface membrane fluidity and cell plasticity. It has postulated that influenza virus infection results in stabilization of the cell surface architecture. These changes are amplified by polyoma virus transformation. Con A acts in this system, as an indicator rather than as a modifier of architectural changes.</p>","PeriodicalId":75854,"journal":{"name":"Folia histochemica et cytochemica","volume":"18 3","pages":"159-72"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17322754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Activity of some respiratory and lysosomal enzymes of lymphocytes in golden hamster with induced melanoma. 金仓鼠诱导黑色素瘤后淋巴细胞部分呼吸酶和溶酶体酶活性的变化。
Folia histochemica et cytochemica Pub Date : 1980-01-01
M Słomianko-Winnicka, W Kuźna-Grygiel
{"title":"Activity of some respiratory and lysosomal enzymes of lymphocytes in golden hamster with induced melanoma.","authors":"M Słomianko-Winnicka,&nbsp;W Kuźna-Grygiel","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A considerable increase in activity of the following enzymes: succinate dehydrogenase (SDH), lactate dehydrogenase (LDH), beta-hydrogenase (beta-HBDH), cytochrome oxidase (CO), and acid phosphatase (AP) was found in lymph nodes lymphocytes after 1 week of malignant melanoma passage in golden hamster (Mesocricetus auratus Waterhouse). The peripheral blood lymphocytes displayed, too, an increase in activity of all the enzymes mentioned except beta-HBDH and SDH. After 3 weeks of the melanoma growth, the animals affected showed a considerable decrease in activity of all the enzymes studied both in the lymph nodes and in the peripheral blood. The increase in enzymatic activity during the initial phase of tumour growth may be a manifestation of biological activation of the cellular defence of lymphocytes. On the other hand, the decrease in the activity seen at the advanced phase of the disease speaks for an impaired immune response.</p>","PeriodicalId":75854,"journal":{"name":"Folia histochemica et cytochemica","volume":"18 4","pages":"303-10"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17322756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preliminary results of the investigations of the cell surface membranes composition (proteins and sugars) in BHK-21, BHK-21/RSV and PR-RSH cell cultures. BHK-21、BHK-21/RSV和PR-RSH细胞表面膜组成(蛋白和糖)的初步研究结果
Folia histochemica et cytochemica Pub Date : 1980-01-01
J Branny, A Klein, M Knycz, C Drózd, J Pawełek
{"title":"Preliminary results of the investigations of the cell surface membranes composition (proteins and sugars) in BHK-21, BHK-21/RSV and PR-RSH cell cultures.","authors":"J Branny,&nbsp;A Klein,&nbsp;M Knycz,&nbsp;C Drózd,&nbsp;J Pawełek","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The results of preliminary investigations concerned with protein and sugar composition of cell surface membranes of BHK-21, BHK-21/RSV and PR-RSH were described. The obtained results indicate on quantitative differences mainly in sugar content of the investigated cells. However differences of quantitative and qualitative composition of proteins of the BHK-21, BHK-21/RSV and PR-RSH were found.</p>","PeriodicalId":75854,"journal":{"name":"Folia histochemica et cytochemica","volume":"18 2","pages":"109-12"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18461478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of intoxication by ceresan on the activity of phosphatases and esterases in the rat thalamus. 酒精中毒对大鼠丘脑磷酸酶和酯酶活性的影响。
Folia histochemica et cytochemica Pub Date : 1980-01-01
J Wigowska-Sowińska
{"title":"The effect of intoxication by ceresan on the activity of phosphatases and esterases in the rat thalamus.","authors":"J Wigowska-Sowińska","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of Ceresan (Methoxyethylmercury chloride) on the histoenzymatic pattern of the thalamus was studied. The experimental animals were treated intragastrically with 100 mg of Ceresan for 6 consecutive days, and the activity of various hydrolases of the thalamus was investigated histochemically. Ingestion of large doses of Ceresan caused widespread alterations in activity of the thalamic hydrolases. A generalized increase of activity was found with respect to TPPase, acP and NsE, while AChE, BuTJ and ATPase activities appeared distinctly reduced. The thalamic neuroglia of experimental rats demonstrated enzymic activities which could not be detected in control animals, and these were: AChE, NsE, acP and TPPase -- in astrocytes, and ATPase -- in oligodendroglia. The changes in the histoenzymatic pattern of the thalamus caused by Ceresan ingestion were topographically highly differentiated. The anterior and lateral nuclei of the thalamus seem to be more sensitive towards Ceresan intoxication than the posterior ones.</p>","PeriodicalId":75854,"journal":{"name":"Folia histochemica et cytochemica","volume":"18 4","pages":"287-96"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17178231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Staining of depolymerised DNA in mammalian tissues with methyl violet 6B and crystal violet. 甲基紫6B和结晶紫对哺乳动物组织中解聚DNA的染色。
Folia histochemica et cytochemica Pub Date : 1980-01-01
M K Dutt
{"title":"Staining of depolymerised DNA in mammalian tissues with methyl violet 6B and crystal violet.","authors":"M K Dutt","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The paper contains an account of DNA staining with basic dyes; methyl violet 6B and crystal violet in mammalian tissue sections after RNA extraction with cold concentrated phosphoric acid. The study shows that the best staining is obtained at pHs 2.5 and 3.5. Dehydration of stained nuclei is perfect when a mixture of absolute ethanol and n-butanol is used followed by treatment of sections in isoamyl or amyl alcohol. The in situ absorption data of nuclei stained with aqueous solution of methyl violet 6B as well as with crystal violet are also presented. Possible mechanism of staining as well as an explanation for dye-leaching when sections are dehydrated through ethanol are discussed.</p>","PeriodicalId":75854,"journal":{"name":"Folia histochemica et cytochemica","volume":"18 1","pages":"79-83"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17220082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enzymes of neutrophils in women with malignant tumors of reproductive organs. 女性生殖器官恶性肿瘤中性粒细胞的酶。
Folia histochemica et cytochemica Pub Date : 1980-01-01
J Blicharski, T Wolska, A Zduńczyk, A Bodzoń, J Lisiewicz, J Piotrowski, K Klimczyk
{"title":"Enzymes of neutrophils in women with malignant tumors of reproductive organs.","authors":"J Blicharski,&nbsp;T Wolska,&nbsp;A Zduńczyk,&nbsp;A Bodzoń,&nbsp;J Lisiewicz,&nbsp;J Piotrowski,&nbsp;K Klimczyk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Enzymatic equipment in peripheral blood neutrophils has been determined in 10 women with cervical carcinoma, 5 with carcinoma of the uterus body and 30 women with myomas of the uterus. Using cytochemical techniques the authors observed the intracellular deficiency of N-acetyl-beta-glucosaminidase accompanied by diminished absolute count of the enzyme-positive cells as well as the beta-glucuronidase- and the myeloperoxidase-positive neutrophils. Lowered activity of the myeloperoxidase was another pattern of cells in question. The acid phosphatase activity of neutrophils in the patients was significantly elevated. It has been suggested that observed intracellular deficiencies of selected enzymes within the neutrophils are of importance with regard to lowered antitumor activity of that cells operating mainly through the myeloperoxidase-H2O2-halide system.</p>","PeriodicalId":75854,"journal":{"name":"Folia histochemica et cytochemica","volume":"18 3","pages":"173-82"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17322755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Additive effects of cortisol and calf serum on RNA synthesis in isolated rat hepatocytes. Autoradiographic studies with the light and electron microscope. 皮质醇和小牛血清对离体大鼠肝细胞RNA合成的影响。用光学和电子显微镜进行放射自显影研究。
Folia histochemica et cytochemica Pub Date : 1980-01-01
M Kwiatkowska, E Puvion, G Moyne
{"title":"Additive effects of cortisol and calf serum on RNA synthesis in isolated rat hepatocytes. Autoradiographic studies with the light and electron microscope.","authors":"M Kwiatkowska,&nbsp;E Puvion,&nbsp;G Moyne","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The known stimulating effect of calf serum (10%) and cortisol (20 microgram/ml) on the incorporation of 2H uridine into the nuclei of isolated rat hepatocytes has been confirmed with the method of light and electron autoradiography. Moreover, it has been shown that cortisol and serum exert additive effects when they are applied jointly. In the presence of either serum or cortisol or both transcriptional activity increases gradually, reaching its maximum level after 6 h incubation. This effect is reduced when the incubation is prolonged up to 24 h. Autoradiographic ultrastructural studies have shown that both serum and cortisol enhance the nucleolar as well as extranucleolar transcriptional activity. The nuclei of hepatocytes cultivated in medium containing serum or both serum and cortisol shown a higher growth rate in comparison with those cultivated in a poor, control medium. Cortisol added to the serum-free medium does not stimulate the nuclear growth.</p>","PeriodicalId":75854,"journal":{"name":"Folia histochemica et cytochemica","volume":"18 4","pages":"249-57"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18217527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of low temperature on the nucleolus of larkspur (Delphinium Ajacis L.) meiocytes. 低温对飞燕减数细胞核仁的影响。
Folia histochemica et cytochemica Pub Date : 1980-01-01
B Gabara
{"title":"Effect of low temperature on the nucleolus of larkspur (Delphinium Ajacis L.) meiocytes.","authors":"B Gabara","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In comparison with stage specific modifications of nucleolus in the early stages of meiotic prophase in Delphinium Ajacis meiocytes, the effect of low temperature is described. On the contrary to control leptotene nucleolus, exhibiting intermingled fibrillar and granular components, cold induced a disappearance of fibrillar material from these organelles. Disappearance of fibrillar material was accompanied by marked diminishment of 3H-uridine incorporation. NOR in control as well as in cold treated material was present. Zygotene nucleolus being in eccentric position displayed segregation of granular and fibrillar components, especially well visible in cold treated cells. In the area occupied in control material by NOR, cold treated cells were found to contain the numerous dark granules. The application of Bernhard's EDTA technique and enzymatic digestion with RNase, DNase and pepsine have proved that the dark granules are composed of RNA. Although pachytene nucleolus still pressed to nuclear envelope exhibited compact structure, composed probably of fibrillar and granular elements, in cold treated meiocytes it showed only the presence of granular component. Moreover, in cold treated cells some dark granules of RNA character were visible at the surface of nucleolus. No was absent. In addition to these changes, in the cytoplasm of cold treated cells in pachytene stage a material of nucleolar origin has appeared. The meiotic irregularities described in the present paper are discussed in relation to the partial male sterility that occurs in Delphinium meiocytes growing in a low temperature.</p>","PeriodicalId":75854,"journal":{"name":"Folia histochemica et cytochemica","volume":"18 4","pages":"259-66"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18217528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Activity of acetylcholine hydrolase (EC 3.1.1.7) in the pig brain; spectrophotometric and histochemical studies in the light and electron microscope. 猪脑乙酰胆碱水解酶(ec3.1.1.7)活性的研究光镜和电子显微镜下的分光光度和组织化学研究。
Folia histochemica et cytochemica Pub Date : 1980-01-01
M Wawrzyniak, R Cybulska, K Radymska-Wawrzyniak
{"title":"Activity of acetylcholine hydrolase (EC 3.1.1.7) in the pig brain; spectrophotometric and histochemical studies in the light and electron microscope.","authors":"M Wawrzyniak,&nbsp;R Cybulska,&nbsp;K Radymska-Wawrzyniak","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>AChE activity (EC 3.1.1.7) in the pig brain was examined by spectrophotometric method and histochemically in the light and electron microscope. The results obtained correlate to a high degree with the existing data concerning the activity of the studied enzyme in other mammals. In the pig, the highest activity was encountered in the striatum nucleus accumbens and nucleus amygdale lateralis: the lowest activity was found in encephalon and cerebellum. In comparison with other mammals AChE activity in the pig brain is considerably lower. No comparable proportions have been encountered between the activities of AChE and ChAT in particular brain regions in the mammals examined so far and also in the pig. The results of histochemical examination both in the light and in the electron microscope correspond to the results obtained by other authors. The discussion deals with the problem of correlation between the level of AChE activity in particular regions of the pig brain with respect to their phylogenetic growth.</p>","PeriodicalId":75854,"journal":{"name":"Folia histochemica et cytochemica","volume":"18 2","pages":"113-22"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18461479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Short-term action of streptozotocin on biochemical activity and morphology of the rat liver golgi apparatus. 链脲佐菌素对大鼠肝脏高尔基体生化活性及形态的短期影响。
Folia histochemica et cytochemica Pub Date : 1980-01-01
A Kordowiak, F Kaczmarski
{"title":"Short-term action of streptozotocin on biochemical activity and morphology of the rat liver golgi apparatus.","authors":"A Kordowiak,&nbsp;F Kaczmarski","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>After six hours, the diabetogenic dose of streptozotocin has neither induced the release of hepatocyte cytoplasmic enzyme nor visibly injured the hepatocyte. As a rule, however, these doses of streptozotocin induced various responses in individual rat livers. Morphological studies have shown hypertrophy of the rat liver Golgi apparatus, higher numbers of secretory vacuoles and dilated cisternae. After this time liver Golgi-rich fractions revealed increased total liver galactosyl transferase activity and higher level but unaltered electrophoretical patterns of protein.</p>","PeriodicalId":75854,"journal":{"name":"Folia histochemica et cytochemica","volume":"18 1","pages":"3-11"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17503021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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