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Folia histochemica et cytochemica最新文献

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Changes in mucus secretion during chemical carcinogenesis in the rat colon. 大鼠结肠化学癌变过程中粘液分泌的变化。
Folia histochemica et cytochemica Pub Date : 1983-01-01
Z Chłap, J Stachura, T Przewłocki, B Mizera, W Rzepecki
{"title":"Changes in mucus secretion during chemical carcinogenesis in the rat colon.","authors":"Z Chłap,&nbsp;J Stachura,&nbsp;T Przewłocki,&nbsp;B Mizera,&nbsp;W Rzepecki","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Adenomatous polyps and adenocarcinomas with variety of the precancerous stages have been induced by intraectally injection of N-methyl-N'-nitro-N-nitrosoguanidine. The aim of our experiments have been to study some quantitative and qualitative changes in mucus secretion both in the neoplasm and in transitional area surrounding tumor. The changes in composition of mucus confirmed by several histochemical methods reflect the earliest morphologically observable stage of malignant transformation in colonic mucosa.</p>","PeriodicalId":75854,"journal":{"name":"Folia histochemica et cytochemica","volume":"21 2","pages":"137-43"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17935295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of vincristine on enzymatic activity in the brain. 长春新碱对大脑酶活性的影响。
Folia histochemica et cytochemica Pub Date : 1983-01-01
M B Kozik, L J Maziarz, J Szczech
{"title":"The effect of vincristine on enzymatic activity in the brain.","authors":"M B Kozik,&nbsp;L J Maziarz,&nbsp;J Szczech","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Adult Wistar rats of either sex were treated intraperitoneally on alternate days with 0.0015 mg of Vincristin over a period of 24 days, and their brains examined with respect to histoenzymatic activity. It has been found that the adopted treatment resulted in a drop of the cerebral AChE, alkP, acP and ATPase activities. The activity of NsE was depressed in some regions of the brain, whereas in others an enhancement of NsE activity was found. TPPase activity appeared to increase following treatment with Vincristin. The observed differential reaction of NsE to Vincristin treatment could be indicative of an existence of multiple isoenzymes of carbocylesterases in the brain. The moderate intensity of changes in the enzymatic activity of the experimental brains evoked by the treatment of rats with Vincristin makes it feasible to assume that after cessation of the treatment the original enzymatic activity of the brain will be restored.</p>","PeriodicalId":75854,"journal":{"name":"Folia histochemica et cytochemica","volume":"21 3-4","pages":"187-94"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17207210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An attempt at comparison of the morphology of normal and RSV--transformed cells in a scanning microscope. 在扫描显微镜下比较正常细胞和RSV转化细胞的形态。
Folia histochemica et cytochemica Pub Date : 1983-01-01
J Branny, J Sukiennik
{"title":"An attempt at comparison of the morphology of normal and RSV--transformed cells in a scanning microscope.","authors":"J Branny,&nbsp;J Sukiennik","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The electronic scanning microscope (SEM) was first used to analyze biological material in 1965 (Hollenberg M. et al. The Journal of Histochem. and Cytochem. 21, 109-130, 1973). It has since been possible to collect specific data on cell morphology and the changes in cell surface and structure caused by external factors, such as oncogenic viruses.</p>","PeriodicalId":75854,"journal":{"name":"Folia histochemica et cytochemica","volume":"21 1","pages":"75-7"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17369363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transfection of brown Leghorn embryo fibroblasts (BLEF) and duck embryo fibroblasts (DEF) by means of DNA isolated from RSV-transformed cells. 用rsv转化细胞分离的DNA转染棕色来角鸭胚胎成纤维细胞(BLEF)和鸭胚胎成纤维细胞(DEF)。
Folia histochemica et cytochemica Pub Date : 1983-01-01
J Branny, M Knycz
{"title":"Transfection of brown Leghorn embryo fibroblasts (BLEF) and duck embryo fibroblasts (DEF) by means of DNA isolated from RSV-transformed cells.","authors":"J Branny,&nbsp;M Knycz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Transfection of Brown Leghorn embryo fibroblasts (BLEF) and embryo fibroblasts (DEF) was made by means of DNA isolated from RSV-transformed cells. DNA samples were carried out by means of phenol method, according to Svoboda at al. [16, 20] from the tumor sarcoma tissue and from cells transformed in vitro (XC and PR-RSH). DNA samples isolated from cell cultures were used for transfection BLEF and DEF cell cultures. Positive transfection results after the use DNA samples were recorded in BLEF and DEF1 cell cultures. The efficiency of transfection of BLEF is about 30% and for DEF1 10%.</p>","PeriodicalId":75854,"journal":{"name":"Folia histochemica et cytochemica","volume":"21 3-4","pages":"273-9"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17382037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of in vitro administration of LH, prolactin separately and LH and prolactin in mixture, on cultured Leydig cells from mouse testes: I. Changes of delta 5, 3 beta-hydroxysteroid dehydrogenase during postnatal life. LH、催乳素单独和LH、催乳素混合体外给药对培养小鼠睾丸间质细胞的影响:1 .出生后δ 5,3 β -羟基类固醇脱氢酶的变化。
Folia histochemica et cytochemica Pub Date : 1983-01-01
B Bilińska
{"title":"Effect of in vitro administration of LH, prolactin separately and LH and prolactin in mixture, on cultured Leydig cells from mouse testes: I. Changes of delta 5, 3 beta-hydroxysteroid dehydrogenase during postnatal life.","authors":"B Bilińska","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Activity of delta 5, 3 beta-hydroxysteroid dehydrogenase in cultured Leydig cells isolated from mouse testes of various age was quantitatively investigated. Activity of delta 5, 3 beta-HSD was low in testes after birth and increased in 21 day old mouse, and then decreased in Leydig cells of 28 day old animals. Maximum activity of dehydrogenase within Leydig cells was observed in 60 day old mice and then gradually declined simultaneously with ageing process. Significant increase of enzymes activity was observed in Leydig cells cultured in medium containing a mixture of 100 ng LH and 100 ng PRL/ml. Doses of either 100 ng LH alone or 100 ng PRL alone showed higher stimulation than lower doses of 10 ng. Changes of activity were statistically significant.</p>","PeriodicalId":75854,"journal":{"name":"Folia histochemica et cytochemica","volume":"21 1","pages":"23-32"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17632566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In memoriam of Władysław Bartoszewicz. 为了纪念Władysław Bartoszewicz。
Folia histochemica et cytochemica Pub Date : 1983-01-01
W Kilarski
{"title":"In memoriam of Władysław Bartoszewicz.","authors":"W Kilarski","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75854,"journal":{"name":"Folia histochemica et cytochemica","volume":"21 3-4","pages":"159-60"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17426669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Glycine and serine rich peptides isolated from rat liver preparations enriched with smooth endoplasmic membranes. 从光滑内质膜丰富的大鼠肝脏制剂中分离的富含甘氨酸和丝氨酸的肽。
Folia histochemica et cytochemica Pub Date : 1983-01-01
M Poźniczek, K Slizowska-Bernaś, M Sarnecka-Keller, I Wybrańska
{"title":"Glycine and serine rich peptides isolated from rat liver preparations enriched with smooth endoplasmic membranes.","authors":"M Poźniczek,&nbsp;K Slizowska-Bernaś,&nbsp;M Sarnecka-Keller,&nbsp;I Wybrańska","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Three electrochromatographic homogeneous peptides belonging to acidic, basic and neutral electrophoretic glycopeptide fractions isolated from rat liver preparations enriched with smooth endoplasmic membranes contain a very high level of glycine and serine and only a slight level of phenylalanine but do not contain tyrosine. Their amino acid compositions differ chiefly in the contents of basic and acidic amino acids.</p>","PeriodicalId":75854,"journal":{"name":"Folia histochemica et cytochemica","volume":"21 2","pages":"101-6"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17470848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dielectric properties of murine lymphocytes. 小鼠淋巴细胞介电特性。
Folia histochemica et cytochemica Pub Date : 1983-01-01
W Jaroszyński, J Terlecki, A Myśliwski, J Myśliwska, J Witkowski
{"title":"Dielectric properties of murine lymphocytes.","authors":"W Jaroszyński,&nbsp;J Terlecki,&nbsp;A Myśliwski,&nbsp;J Myśliwska,&nbsp;J Witkowski","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Dielectric measurements in frequency range from 0.032 up to 110 MHZ were performed on the suspension of lymphocytes isolated from the spleen of Balb/c mouse. Basing on measurements of electric capacitance (C) and electric conductance (G) of lymphocyte suspension and on the simplified Pauly-Schwan equations the following electrical parameters of lymphocytes were calculated: relative electrical permittivity of the intracellular substance (epsilon i = 136) electrical conductivity of the inner phase (intracellular substance) (kappa i = 8.94 mS/cm), specific capacitance of cell membrane (CM = 0.82 microF/cm2). Relative electrical permittivity of the membrane (epsilon s = 4.6) has also been calculated. Two of those quantities (epsilon i = and kappa i) additionally determined by means of empirical dependences. The value of epsilon i and kappa i were respectively 125 and 8.79 mS/cm. All those measurements were taken at temperature amounting to 293 K.</p>","PeriodicalId":75854,"journal":{"name":"Folia histochemica et cytochemica","volume":"21 3-4","pages":"161-72"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17722445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Aggregates of microfilaments in astrocytes and fibroblasts in tissue culture in vitro as revealed with Coomassie brilliant blue R 250. 用考马斯亮蓝r250观察体外组织培养的星形胶质细胞和成纤维细胞中微丝的聚集。
Folia histochemica et cytochemica Pub Date : 1983-01-01
J Kałuza, J Stachura, R Kucharska
{"title":"Aggregates of microfilaments in astrocytes and fibroblasts in tissue culture in vitro as revealed with Coomassie brilliant blue R 250.","authors":"J Kałuza,&nbsp;J Stachura,&nbsp;R Kucharska","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In tissue culture in vitro in fibroblasts cultivated on glass coverslips numerous aggregates of microfilaments demonstrating the typically parallel arrangement and originating intercellular connections were found. In contrast to the structural microfilamentous organization of fibroblasts, in glial cells of astrocyte type the microfilament aggregates are short and do not demonstrate a tendency to structural organization. The Coomassie blue positive substances either form granules of various size or are of irregular form and sometimes of rod -like appearance. In astrocyte-like cells of neoplastic origin no elements of the cytoskeleton could be demonstrated by this method. The differences in the structural organization of the cytoskeleton of mesodermal and neuroectodermal cells are discussed.</p>","PeriodicalId":75854,"journal":{"name":"Folia histochemica et cytochemica","volume":"21 3-4","pages":"281-5"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17742704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of lead poisoning on ultrastructural changes in the body wall of Eisenia foetida (Savigny), Oligochaeta. II. Long action of different concentration of lead on ultrastructural changes in the cells of the body wall. 铅中毒对寡毛藻藻壁超微结构变化的影响。2不同浓度铅对体壁细胞超微结构变化的长期作用。
Folia histochemica et cytochemica Pub Date : 1983-01-01
E Wielgus-Serafińska, M Strzelec
{"title":"Influence of lead poisoning on ultrastructural changes in the body wall of Eisenia foetida (Savigny), Oligochaeta. II. Long action of different concentration of lead on ultrastructural changes in the cells of the body wall.","authors":"E Wielgus-Serafińska,&nbsp;M Strzelec","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The studies were performed on earthworms collected from March to September in the vicinity of Katowice. The earthworms were breed in Petri dishes on soil to which lead nitrate was added in the following concentrations 0.025, 0.10, 0.50 mg/g of soil. For the performed studies small specimen were taken from behind the citellum in which was lead histochemically evaluated. Tissue specimen taken from the citellum of the experimental animals were investigated in electronmicroscope. It has been found that lead accumulates in the sarcoplasm of the muscle fibres but not in the cell organells. Lead concentrations in the cells appear as regular crystals with membranaceous cover. The lead compounds used in the experiments does not provoke structural changes in the contractile elements of the muscle fibres, they induce only some deformations of mitochondria and hyperactivity of the glandular cells of the epithelium.</p>","PeriodicalId":75854,"journal":{"name":"Folia histochemica et cytochemica","volume":"21 2","pages":"145-51"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17935296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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