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Current topics in molecular endocrinology最新文献

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Purification and characterization of rabbit testicular androgen binding protein (ABP). 兔睾丸雄激素结合蛋白(ABP)的纯化与特性研究。
Current topics in molecular endocrinology Pub Date : 1975-01-01 DOI: 10.1007/978-1-4613-4440-7_31
S C Weddington, P Brandtzaeg, K Sletten, T Christensen, V Hansson
{"title":"Purification and characterization of rabbit testicular androgen binding protein (ABP).","authors":"S C Weddington,&nbsp;P Brandtzaeg,&nbsp;K Sletten,&nbsp;T Christensen,&nbsp;V Hansson","doi":"10.1007/978-1-4613-4440-7_31","DOIUrl":"https://doi.org/10.1007/978-1-4613-4440-7_31","url":null,"abstract":"<p><p>Testicular androgen binding protein (ABP) was purified from the epididymis of 1500 adult rabbits by the sequential use of ammonium sulphate precipitation, ion exchange chromatography on DEAE cellulose, gel filtration on Sephadex G-200, hydroxyl-apatite chromatography and preparative polyacrylamide gel electrophoresis. This procedure yielded a 1000-fold increase in specific activity compared to that of the 1500,000 x g supernatant, and the recovery of active ABP was about 3-5%. ABP is acid glycoprotein with a molecular weight of 65-68,000 daltons. Antisera to rabbit ABP raised in quinea pigs inhibit 3H-DHT binding to ABP as measured by SS-PAGE. When diluted rabbit serum containing TeBG is treated with the same dilutions of these antisera, identical binding inhibition curves are found. Thus, ABP and TeBG in rabbits appear to possess identical immunological determinants.</p>","PeriodicalId":75766,"journal":{"name":"Current topics in molecular endocrinology","volume":"2 ","pages":"433-51"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12418963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Follicle stimulating hormone and cyclic AMP directed changes in ultrastructure and properties of cultured Sertoli cell-enriched preparations: comparison with cultured testicular peritubular cells. 促卵泡激素和环AMP对培养的支持细胞富集制剂超微结构和性质的影响:与培养的睾丸小管周围细胞的比较。
Current topics in molecular endocrinology Pub Date : 1975-01-01 DOI: 10.1007/978-1-4613-4440-7_34
P. S. Tung, I. Fritz
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引用次数: 17
Perfusion of the male rat reproductive tract: a model for the study of androgen secretion and action. 雄性大鼠生殖道灌注:研究雄激素分泌及其作用的模型。
Current topics in molecular endocrinology Pub Date : 1975-01-01 DOI: 10.1007/978-1-4613-4440-7_10
H W Baker, C W Bardin, G J Eichner, L S Jefferson, R J Santen
{"title":"Perfusion of the male rat reproductive tract: a model for the study of androgen secretion and action.","authors":"H W Baker,&nbsp;C W Bardin,&nbsp;G J Eichner,&nbsp;L S Jefferson,&nbsp;R J Santen","doi":"10.1007/978-1-4613-4440-7_10","DOIUrl":"https://doi.org/10.1007/978-1-4613-4440-7_10","url":null,"abstract":"","PeriodicalId":75766,"journal":{"name":"Current topics in molecular endocrinology","volume":"2 ","pages":"135-44"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12412684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Follicle stimulating hormone and cyclic AMP directed changes in ultrastructure and properties of cultured Sertoli cell-enriched preparations: comparison with cultured testicular peritubular cells. 促卵泡激素和环AMP对培养的支持细胞富集制剂超微结构和性质的影响:与培养的睾丸小管周围细胞的比较。
Current topics in molecular endocrinology Pub Date : 1975-01-01
P S Tung, I B Fritz
{"title":"Follicle stimulating hormone and cyclic AMP directed changes in ultrastructure and properties of cultured Sertoli cell-enriched preparations: comparison with cultured testicular peritubular cells.","authors":"P S Tung,&nbsp;I B Fritz","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75766,"journal":{"name":"Current topics in molecular endocrinology","volume":"2 ","pages":"459-508"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11411703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The blood - testis barrier and steroids. 血睾丸屏障和类固醇。
Current topics in molecular endocrinology Pub Date : 1975-01-01 DOI: 10.1007/978-1-4613-4440-7_16
B P Setchell, S J Main
{"title":"The blood - testis barrier and steroids.","authors":"B P Setchell,&nbsp;S J Main","doi":"10.1007/978-1-4613-4440-7_16","DOIUrl":"https://doi.org/10.1007/978-1-4613-4440-7_16","url":null,"abstract":"","PeriodicalId":75766,"journal":{"name":"Current topics in molecular endocrinology","volume":"2 ","pages":"223-33"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12412687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 24
Androgen receptor in rat testis. 大鼠睾丸中的雄激素受体。
Current topics in molecular endocrinology Pub Date : 1975-01-01 DOI: 10.1007/978-1-4613-4440-7_18
A A Smith, W S McLean, S N Nayfeh, F S French
{"title":"Androgen receptor in rat testis.","authors":"A A Smith,&nbsp;W S McLean,&nbsp;S N Nayfeh,&nbsp;F S French","doi":"10.1007/978-1-4613-4440-7_18","DOIUrl":"https://doi.org/10.1007/978-1-4613-4440-7_18","url":null,"abstract":"","PeriodicalId":75766,"journal":{"name":"Current topics in molecular endocrinology","volume":"2 ","pages":"257-80"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12412689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Androgen receptors in testis tissue enriched in Sertoli cells. 睾丸组织中的雄激素受体在支持细胞中富集。
Current topics in molecular endocrinology Pub Date : 1975-01-01 DOI: 10.1007/978-1-4613-4440-7_20
E Mulder, M J Peters, H J van der Molen
{"title":"Androgen receptors in testis tissue enriched in Sertoli cells.","authors":"E Mulder,&nbsp;M J Peters,&nbsp;H J van der Molen","doi":"10.1007/978-1-4613-4440-7_20","DOIUrl":"https://doi.org/10.1007/978-1-4613-4440-7_20","url":null,"abstract":"","PeriodicalId":75766,"journal":{"name":"Current topics in molecular endocrinology","volume":"2 ","pages":"287-91"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12412691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Some factors affecting testosterone, dihydrotestosterone, 5 alpha-androstan-3 alpha,17 beta-diol and 5 alpha-androstan-3 beta,17 beta-diol secretion by invitro perfused rabbit testes. 体外灌注兔睾丸分泌睾酮、二氢睾酮、5 -雄激素-3 α、17 -二醇及5 -雄激素-3 β、17 -二醇的影响因素
Current topics in molecular endocrinology Pub Date : 1975-01-01
L L Ewing
{"title":"Some factors affecting testosterone, dihydrotestosterone, 5 alpha-androstan-3 alpha,17 beta-diol and 5 alpha-androstan-3 beta,17 beta-diol secretion by invitro perfused rabbit testes.","authors":"L L Ewing","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Intra-arterial infusion of testosterone-3H gave rise to tritiated dihydrotestosterone, 5 alpha-androstan-3 alpha,17 beta-diol and 5 alpha-androstan-3beta,17 beta-diol in spermatic venous effluent of the perfused rabbit testis-epididymis. Mass spectrometric measurements confirmed that these four androgens were present in spermatic venous effluent of the perfused rabbit testis-epididymis. Gas liquid chromatographic measurement showed that testosterone, dihydrotestosterone, 5 alpha-androstan-3 alpha,17 beta-diol and 5 alpha-androstan-3 beta,17 beta-diol were secreted in similar amounts by the in vitro perfused and in situ rabbit testis-epididymis results obtained by perfusing the testis minus the epididymis suggested that the bulk of these androgens originate from the catabolism of testosterone within the testis rather than the epididymis. Suprisingly, germinal epithelium destruction by heat failed to alter the testosterone, dihydrotestosterone and 5 alpha-androstan-3 alpha,17 beta-diol secretion by the in vitro perfused rabbit testis. In contrast, the secretion of 5 alpha-androstan-3 beta,17 beta-diol was significantly (P less than 0.05) reduced in the same cryptorchid compared to control testes.</p>","PeriodicalId":75766,"journal":{"name":"Current topics in molecular endocrinology","volume":"2 ","pages":"145-64"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11241151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Stimulation by follicle stimulating hormone and dibutyryl cyclic AMP of incorporation of 3H-thymidine into nuclear DNA of cultured Sertoli cell-enriched preparations from immature rats. 促卵泡激素和二丁基环AMP对未成熟大鼠培养的支持细胞富集物中3h -胸腺嘧啶掺入核DNA的刺激作用。
Current topics in molecular endocrinology Pub Date : 1975-01-01 DOI: 10.1007/978-1-4613-4440-7_29
M Griswold, E Mably, I B Fritz
{"title":"Stimulation by follicle stimulating hormone and dibutyryl cyclic AMP of incorporation of 3H-thymidine into nuclear DNA of cultured Sertoli cell-enriched preparations from immature rats.","authors":"M Griswold,&nbsp;E Mably,&nbsp;I B Fritz","doi":"10.1007/978-1-4613-4440-7_29","DOIUrl":"https://doi.org/10.1007/978-1-4613-4440-7_29","url":null,"abstract":"","PeriodicalId":75766,"journal":{"name":"Current topics in molecular endocrinology","volume":"2 ","pages":"413-20"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-4613-4440-7_29","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11411702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
Culture and FSH responses of Sertoli cells isolated from sexually mature rat testis. 性成熟大鼠睾丸支持细胞的培养及卵泡刺激素反应。
Current topics in molecular endocrinology Pub Date : 1975-01-01 DOI: 10.1007/978-1-4613-4440-7_28
A Steinberger, J S Elkington, B M Sanborn, E Steinberger
{"title":"Culture and FSH responses of Sertoli cells isolated from sexually mature rat testis.","authors":"A Steinberger,&nbsp;J S Elkington,&nbsp;B M Sanborn,&nbsp;E Steinberger","doi":"10.1007/978-1-4613-4440-7_28","DOIUrl":"https://doi.org/10.1007/978-1-4613-4440-7_28","url":null,"abstract":"","PeriodicalId":75766,"journal":{"name":"Current topics in molecular endocrinology","volume":"2 ","pages":"399-411"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11411701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 34
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