Cell biology international reports最新文献_第8页

Cell biology international reports Pub Date : 1992-03-01 DOI: 10.1016/S0309-1651(06)80132-3

DAC

引用次数: 0

Swelling response of golgi apparatus cisternae in cells treated with monensin is reduced by cell injury 细胞损伤后高尔基体池肿胀反应减弱

Cell biology international reports Pub Date : 1992-03-01 DOI: 10.1016/S0309-1651(06)80123-2

Hilton H. Mollenhauer , D. James Morré , Nita Minnifield

{"title":"Swelling response of golgi apparatus cisternae in cells treated with monensin is reduced by cell injury","authors":"Hilton H. Mollenhauer , D. James Morré , Nita Minnifield","doi":"10.1016/S0309-1651(06)80123-2","DOIUrl":"10.1016/S0309-1651(06)80123-2","url":null,"abstract":"<div><p>The effect of mechanical stress on Golgi apparatus was examined in thin slices of rat liver. The findings should be of relevance both to electron microscopists who routinely mince tissue, and to biochemists who homogenize tissues to isolate membranous components. The swelling response of Golgi apparatus to monensin was used as an assay because the swelling response is distinct and is thought to result from a well-characterized metabolic process, namely the acidification of vesicles. The results showed that the swelling response was compromised by monensin as far away as 6–7 cells from a cut surface even though other aspects of cell ultrastructure were not altered from normal. The monensin-induced swelling response was also evaluated in isolated Golgi apparatus and found to be similar to that with tissue. Thus, mechanical stress such as commonly used to mince tissue or isolate tissue components, appears to markedly alter Golgi apparatus function compared to the situation <em>in vivo</em>. In this example, the altered response of Golgi apparatus to monensin indicated that some aspects associated with the ATP-dependant proton-pumping machinery of the transmost cisternae and trans Golgi network were compromised.</p></div>","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 3","pages":"Pages 217-220"},"PeriodicalIF":0.0,"publicationDate":"1992-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80123-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12748928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Histone variants during sea urchin development 海胆发育过程中的组蛋白变异

Cell biology international reports Pub Date : 1992-03-01 DOI: 10.1016/S0309-1651(06)80121-9

Giuseppe Romano

引用次数: 11

Treatment of porcine aortic smooth muscle cells with phorbol esters prevents nodulation in vitro 用佛波酯处理猪主动脉平滑肌细胞可防止体外结瘤

Cell biology international reports Pub Date : 1992-03-01 DOI: 10.1016/S0309-1651(06)80124-4

Christian Sell , Paul Held , Kilambi Janakidevi

引用次数: 4

Role of fibronectin in the organisation of the cytoskeleton during the sreading of rat mammary epithelial cells 大鼠乳腺上皮细胞扩散过程中纤维连接蛋白在细胞骨架组织中的作用

Cell biology international reports Pub Date : 1992-03-01 DOI: 10.1016/S0309-1651(06)80122-0

Sharon A. Ferns, Rosemary Kimbell, Jane A. Aitken, Michael J. Warburton

{"title":"Role of fibronectin in the organisation of the cytoskeleton during the sreading of rat mammary epithelial cells","authors":"Sharon A. Ferns, Rosemary Kimbell, Jane A. Aitken, Michael J. Warburton","doi":"10.1016/S0309-1651(06)80122-0","DOIUrl":"10.1016/S0309-1651(06)80122-0","url":null,"abstract":"<div><p>The correlation between the extracellular deposition of fibronectin and the development of the actin-containing cytoskeleton was studied during the attachment and spreading of the rat mammary epithelial cell line Rama 25. During the initial phase of cell spreading, actin is localised in peripheral microfilament bundles. As cell spreading increases, the peripheral ring is displaced towards the perinuclear region. Fibronectin, deposited beneath the basal surface, co-localises with the actin-containing peripheral ring. The peripheral ring subsequently disappears and is replaced by a system of radial microfilaments that extend from the perinuclear region to the cell periphery. At this stage, there is no correlation between the distribution of fibronectin and actin. As cells form colonies, radial microfilament bundles are replaced by peripheral microfilament bundles which do not co-localise with fibronectin. Cells at the edges of colonies extend lamellae that contain microfilament stress fibres. In these structures there is co-localisation of actin, fibronectin and the a5ß1-integrin fibronectin receptor.</p></div>","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 3","pages":"Pages 207-216"},"PeriodicalIF":0.0,"publicationDate":"1992-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80122-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12702807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

神经系统的精细结构，第三版，收录于:神经元及其支持细胞。牛津大学出版社(1991)，494页。£80.00。, 0-19-506571-9

Cell biology international reports Pub Date : 1992-03-01 DOI: 10.1016/S0309-1651(06)80129-3

A Peters, SL Palay, HdeF Webster, CH

引用次数: 13

Primary culture of liver cancer tissues with or without transcatheter arterial embolization and establishment of a cell strain 经导管动脉栓塞或不经导管动脉栓塞的肝癌组织原代培养及细胞株的建立

Cell biology international reports Pub Date : 1992-03-01 DOI: 10.1016/S0309-1651(06)80127-X

Tokiwa T. , Kusaka Y. , Endo A. , Namba M.

引用次数: 1

Differential responses of lignin derivatives between tumor and normal tissue derived cell lines; Effects on cellular adhesion and cell growth 木质素衍生物在肿瘤和正常组织来源细胞系间的差异反应对细胞粘附和细胞生长的影响

Cell biology international reports Pub Date : 1992-03-01 DOI: 10.1016/S0309-1651(06)80126-8

Kenji Sorimachi

引用次数: 4

Meetings and conferences 会议和会议

Cell biology international reports Pub Date : 1992-03-01 DOI: 10.1016/S0309-1651(06)80134-7

引用次数: 0

Effects of platelet-derived growth factor on human and mouse osteoblastic cells isolated from the trabecular bone surface 血小板源性生长因子对人、鼠骨小梁表面成骨细胞的影响

Cell biology international reports Pub Date : 1992-03-01 DOI: 10.1016/S0309-1651(06)80125-6

Boutaina Abdennagy, Monique Hott, Pierre J. Marie

{"title":"Effects of platelet-derived growth factor on human and mouse osteoblastic cells isolated from the trabecular bone surface","authors":"Boutaina Abdennagy, Monique Hott, Pierre J. Marie","doi":"10.1016/S0309-1651(06)80125-6","DOIUrl":"10.1016/S0309-1651(06)80125-6","url":null,"abstract":"<div><p>We show here that purified platelet derived growth factor (PDGF) stimulates DNA synthesis in normal endosteal mouse and human osteoblastic cells isolated by selective migration from the trabecular bone surface. Maximum DNA synthesis as measured by (<sup>3</sup>H)-thymidine incorporation into DNA was increased at 50 ng/ml PDGF (48–72 hours). In both species, the effect of PDGF (25ng/ml) was lower than the mitogenic effect of 10%FCS. We found that the mitogenic effect of PDGF on human trabecular cells decreased with the number of cell passages. DNA synthesis was increased about 4-fold by PDGF (25 ng/ml) in early passaged cells that expressed low basal growth rate and high osteocalcin production in basal conditions and in response to 1,25(OH)<sub>2</sub> vitamin D, whereas DNA synthesis was increased 1.2 fold by PDGF in late passaged cells that showed high basal growth rate and low osteocalcin release in absence or presence of 1,25(OH)<sub>2</sub> D. PDGF alone had no effect on osteocalcin production. These results indicate that PDGF has mitogenic effect on normal mouse and human osteoblastic cells lining the trabecular bone surface and that the responsiveness to PDGF of human trabecular cells varies with the stage of differentiation.</p></div>","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 3","pages":"Pages 235-247"},"PeriodicalIF":0.0,"publicationDate":"1992-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80125-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12748930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14