Brookhaven symposia in biology最新文献

The meaning of stability. 稳定的意义。

Brookhaven symposia in biology Pub Date : 2020-07-27 DOI: 10.2307/j.ctt1wvwdr9.84

R. Lewontin

引用次数: 303

Mechanisms of DNA uptake by cells. 细胞摄取DNA的机制。

Brookhaven symposia in biology Pub Date : 1977-05-12

S A Lacks

引用次数: 0

The structure of late adenovirus type 2 messenger RNAs. 晚期腺病毒2型信使rna的结构。

Brookhaven symposia in biology Pub Date : 1977-05-12

R E Gelinas, L T Chow, R J Roberts, T R Broker, D F Klessig

引用次数: 0

Teratocarcinoma cells as vehicles for mutant and foreign genes. 畸胎癌细胞作为突变基因和外源基因的载体。

Brookhaven symposia in biology Pub Date : 1977-05-12

B Mintz

{"title":"Teratocarcinoma cells as vehicles for mutant and foreign genes.","authors":"B Mintz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Mouse malignant teratocarcinoma cells, upon injection into early embryos at the blastocyst stage, become integrated into the developing embryo and stably normalized. In the mosaic mice that are formed, tumor-derived cells can give rise to the full range of normal, functional somatic tissues, and also to germ cells from which progeny are obtained. Totipotent mouse teratocarcinoma cells thus provide a new and essentially parasexual means of generating entire animals. The advantages of in vitro and in vivo technologies can now therefore be combined to synthesize mice with experimentally useful mutations. After mutagenization of the teratocarcinoma cells in culture, and selection or screening for the desired mutant phenotype, cells of mutant clones are microinjected into genetically marked blastocysts for further differentiation and full gene expression within the framework of the organism. An example is the isolation, by 6-thioguanine resistance, of cells deficient in hypoxanthine-guanine phosphoribosyl transferase -- the same deficiency that characterizes human patients with the X-linked Lesch-Nyhan disease. In work in progress, these cells have been cycled into genetically marked blastocysts, where the tumor lineage has successfully given rise to fully differentiated tissue contributions in which the enzyme defect persists. Such experiments present numerous possibilities for introducing specific mutations into mice, toward the ends of studying gene mechanisms responsible for differentiation and of producing animal models of human genetic diseases. Teratocarcinoma cells may also serve as vehicles for introducing foreign genetic material into mice in order to facilitate analyses of gene control mechanisms in development and disease.</p>","PeriodicalId":75624,"journal":{"name":"Brookhaven symposia in biology","volume":" 29","pages":"82-95"},"PeriodicalIF":0.0,"publicationDate":"1977-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11955052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Injection of tRNAs into somatic cells: search for in vivo systems to assay potential nonsense mutations in somatic cells. 向体细胞注射trna:寻找体内系统来测定体细胞中潜在的无义突变。

Brookhaven symposia in biology Pub Date : 1977-05-12

J E Celis

引用次数: 0

Gene transfer experiments in Neurospora. 神经孢子虫基因转移实验。

Brookhaven symposia in biology Pub Date : 1977-05-12

N C Mishra

引用次数: 0

The expression of cloned eukaryotic DNA in prokaryotes. 克隆真核DNA在原核生物中的表达。

Brookhaven symposia in biology Pub Date : 1977-05-12

J Carbon, B Ratzkin, L Clarke, D Richardson