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Bollettino dell'Istituto sieroterapico milanese最新文献

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HTLV-I and HIV-I antibody frequency in thalassemics and drug addicts in southeast Sicily. 西西里岛东南部地中海贫血患者和吸毒者中HTLV-I和HIV-I抗体频率。
Bollettino dell'Istituto sieroterapico milanese Pub Date : 1991-01-01
G Gulisano, M Ciccia, M Ienna, A Cipolla, L Mauro
{"title":"HTLV-I and HIV-I antibody frequency in thalassemics and drug addicts in southeast Sicily.","authors":"G Gulisano,&nbsp;M Ciccia,&nbsp;M Ienna,&nbsp;A Cipolla,&nbsp;L Mauro","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>HTLV-I (Human T-Lymphotropic Virus Type 1) and HIV-1 (Human Immunodeficiency Virus Type 1) antibodies have been investigated in 702 sera samples from: 259 drug addicts nerve screened for HIV-1 (Group I), 43 drug addicts already identified as HIV-1 seropositive (Group II), 222 thalassemics (Group III) and 178 occasional blood donors (Group IV). The sera, collected from June 1984 to April 1989, were screened a first time with immunoenzymatic test and the samples proving positive were then confirmed by Western blot test. The seropositive frequency with Western blot for anti-HTLV I was 2.7% in Group I and 6.9% in Group II. Of the thalassemics, five of whom had been previously identified as seropositive for HIV-1, and of the blood donors, none proved to be seropositive for HTLV-I antibodies. With regard to HIV-1, a positive antibody response to the Western blot was found in 19.9% of the Group I subjects. Contemporaneous presence of HTLV-I and HIV-1 antibodies was found in five subjects.</p>","PeriodicalId":75613,"journal":{"name":"Bollettino dell'Istituto sieroterapico milanese","volume":"70 1-2","pages":"531-4"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12835571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Semiquantitative PCR in the diagnosis of HIV infection: correlation to clinical stage and serological status. 半定量PCR在HIV感染诊断中的应用:与临床分期和血清学状态的关系。
Bollettino dell'Istituto sieroterapico milanese Pub Date : 1991-01-01
G V De Socio, C Falcinelli, D Francisci, S Pauluzzi, E V Cosmi, M M Anceschi
{"title":"Semiquantitative PCR in the diagnosis of HIV infection: correlation to clinical stage and serological status.","authors":"G V De Socio,&nbsp;C Falcinelli,&nbsp;D Francisci,&nbsp;S Pauluzzi,&nbsp;E V Cosmi,&nbsp;M M Anceschi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Authors utilized the polymerase chain reaction (PCR) technique to detect the presence of HIV DNA in 31 subjects (12 seropositive patients, 4 seronegative, at risk persons and 15 seronegative, not at risk controls). PCR was highly sensitive (enabling the detection of as few as 10 proviral genomes) and specific. By comparison to known amounts of HIV DNA, it was possible to obtain semiquantitative evaluation. No correlation was found between the proviral amount and the clinical stage of the disease or the p24 antigenemia.</p>","PeriodicalId":75613,"journal":{"name":"Bollettino dell'Istituto sieroterapico milanese","volume":"70 1-2","pages":"403-7"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12835848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Levels of interleukin-4 (IL-4) in lymphocyte cultures of subjects with HIV-1 infection]. [白细胞介素-4 (IL-4)在HIV-1感染者淋巴细胞培养中的水平]。
Bollettino dell'Istituto sieroterapico milanese Pub Date : 1991-01-01
O Perrella, G Liguori, E Precchia, E Soscia, D Guarnaccia, I Caruso, P Marinelli, L Finelli, G Tartaglia
{"title":"[Levels of interleukin-4 (IL-4) in lymphocyte cultures of subjects with HIV-1 infection].","authors":"O Perrella,&nbsp;G Liguori,&nbsp;E Precchia,&nbsp;E Soscia,&nbsp;D Guarnaccia,&nbsp;I Caruso,&nbsp;P Marinelli,&nbsp;L Finelli,&nbsp;G Tartaglia","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The authors report IL-4 levels in peripheral blood mononuclear cells (PBMC), stimulated with MoAb anti-CD3, in subjects with HIV-1 infection, classified according to the criteria of Centers for Disease Control (CDC) in various stages. The results \"in vitro\" have indicate that PBMC IL-4 levels were in normal range also in advanced stages of retroviral infection, and were correlated to low levels of CD4+ lymphocytes and, sometimes, to high levels of IgE immunoglobulins. The normality of IL-4 levels in advanced stages or in presence of decreased CD4+ cells, suggests that the production of this cytokine is also present in other subsets of mononuclear cells not involved in this infection. Moreover the presence of significant correlation between high levels of Ig E and IL-4 suggests a possible pathogenetic role of IL-4 in the determination of isotypic switch of this immunoglobulin.</p>","PeriodicalId":75613,"journal":{"name":"Bollettino dell'Istituto sieroterapico milanese","volume":"70 1-2","pages":"471-4"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12852538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chlamydia trachomatis infection in "sine causa" recurrent abortion. 沙眼衣原体感染在“正因性”复发性流产中的作用。
Bollettino dell'Istituto sieroterapico milanese Pub Date : 1991-01-01
P Olliaro, A Regazzetti, G Gorini, F Milano, A Marchetti, E G Rondanelli
{"title":"Chlamydia trachomatis infection in \"sine causa\" recurrent abortion.","authors":"P Olliaro,&nbsp;A Regazzetti,&nbsp;G Gorini,&nbsp;F Milano,&nbsp;A Marchetti,&nbsp;E G Rondanelli","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>One hundred and one women suffering from \"sine causa\" recurrent abortion were screened for Chlamydia trachomatis (C.T.) infection by using direct examination, cultural and serological procedures. In this series, C.T. infection did not appear to be related to increased risk of recurrent abortion. The culture-positive and serology-positive rates (14.85% and 34.65%, respectively) did not differ from other unselected populations. Neither time from last abortion nor type of abortion were significantly related to C.T. infection. Nonetheless, the women who underwent examination within one year from last abortion and had a culture-positive partner as well, were more likely to present with a C.T.-positive culture.</p>","PeriodicalId":75613,"journal":{"name":"Bollettino dell'Istituto sieroterapico milanese","volume":"70 1-2","pages":"467-70"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12890491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of immunoglobulin M-antibodies after rubella immunization with RA27/3 attenuated live vaccine in teen-agers: remaining problems. 青少年风疹减毒活疫苗RA27/3免疫后免疫球蛋白m抗体的发展:遗留问题
Bollettino dell'Istituto sieroterapico milanese Pub Date : 1991-01-01
A Braito, O Fanti, G M Rossolini, C Cellesi
{"title":"Development of immunoglobulin M-antibodies after rubella immunization with RA27/3 attenuated live vaccine in teen-agers: remaining problems.","authors":"A Braito,&nbsp;O Fanti,&nbsp;G M Rossolini,&nbsp;C Cellesi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Four-hundred-fourty-two rubella seronegative teen-agers living in Siena and the surroundings were vaccinated with RA 27/3 live attenuated rubella over a two years period (1985-1987). One-hundred-twenty seven vaccinees' sera were randomly selected for the rubella specific antibodies of the IgM class detection with two Elisa Methods; the sera were collected 10 and 30 days after the challenge; in 58 girls a serum sample was also obtained 1 yr later. None of the sera showed reactivity for RF. The comparison between the two Elisa methods gave satisfactory results, the percentage of discrepancies being low (3.9%). Ten days after the immunization the IgM positive sera were only 7.56% (Elisa Abbott) and 5.88% (Elisa Enzygnost Behringwerke). One month later, 86.61% of the specimens showed reactivity for specific IgM antibodies. None of 58 sera gave positive results after 1 yr. Rubella antibodies of the IgG class could be demonstrated in the \"screening\" sera of 3 IgM negative subjects, when an Elisa method was applied. Nine subjects (7%) repeatedly resulted IgM negative one month after the challenge although a seroconversion was observed using both HAI and EIA. A serum sample was also obtained in 6/9 IgM \"non responders\" after a rubella epidemic observed in 1988-1989; no subclinical reinfection due to the wild virus was detected in this group, no changes in HAI antibody title nor evidence of IgM class specific response were observed. Although most of the diagnostic aspects related with rubella and rubella vaccination appear to be resolved, it should be underlined that there are several remaining problems especially associated with IgM detection.</p>","PeriodicalId":75613,"journal":{"name":"Bollettino dell'Istituto sieroterapico milanese","volume":"70 1-2","pages":"443-8"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12835852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Recent data on the spread of Listeria spp. in the province of Ferrara]. [费拉拉省李斯特菌传播的最新数据]。
Bollettino dell'Istituto sieroterapico milanese Pub Date : 1991-01-01
G Bucci, P Maini, E Contato
{"title":"[Recent data on the spread of Listeria spp. in the province of Ferrara].","authors":"G Bucci,&nbsp;P Maini,&nbsp;E Contato","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The results of the investigations of Listeria held from 1985 to 1989 in samples of foods, surface waters and stools are reported. On the whole 98 strains of Listeria were isolated, 38 of which were L. monocytogenes. The first isolation of L. grayi from human stools is pointed out.</p>","PeriodicalId":75613,"journal":{"name":"Bollettino dell'Istituto sieroterapico milanese","volume":"70 1-2","pages":"495-8"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12834846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Survival of bacteriophage 1 of the Salmonella typhimurium phage typing scheme in 4 different types of sterile soil. 鼠伤寒沙门菌噬菌体分型方案噬菌体1在4种不同类型无菌土壤中的存活。
Bollettino dell'Istituto sieroterapico milanese Pub Date : 1991-01-01
J Leonardopoulos, A Papaconstantinou, J Papavassiliou
{"title":"Survival of bacteriophage 1 of the Salmonella typhimurium phage typing scheme in 4 different types of sterile soil.","authors":"J Leonardopoulos,&nbsp;A Papaconstantinou,&nbsp;J Papavassiliou","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The survival of bacteriophage 1 of the Salmonella typhimurium phage typing scheme was studied in four different types of sterile soil at 4.20 and 37 degrees C. The longevity of the phage was generally short, not exceeding 36 days and depended on the temperature and the type of soil.</p>","PeriodicalId":75613,"journal":{"name":"Bollettino dell'Istituto sieroterapico milanese","volume":"70 1-2","pages":"505-12"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12834849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Decline of HBsAg antibodies after immunization with hepatitis B vaccines and after natural infection. 乙型肝炎疫苗免疫后和自然感染后HBsAg抗体下降。
Bollettino dell'Istituto sieroterapico milanese Pub Date : 1991-01-01
A Braito, A M Bianchini, C Mattei, A Benci, D Marri, M Toti
{"title":"Decline of HBsAg antibodies after immunization with hepatitis B vaccines and after natural infection.","authors":"A Braito,&nbsp;A M Bianchini,&nbsp;C Mattei,&nbsp;A Benci,&nbsp;D Marri,&nbsp;M Toti","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The long-term persistence of anti-HBsAg above 10 mUI is conventionally believed to protect against natural infection with hepatitis B virus, while it is not yet clear what is the clinical significance of the fall to below 10 mUI in antibody levels. In our opinion, an important method for evaluating the effectiveness of the vaccine lies in comparing the duration of vaccine-immunity with that following the disease, the later being held to provide life-long protection. In this view, we examined the sera samples of 69 subjects (56 healthy people, and 13 drug-addicts) previously affected from HBV infection looking for anti-HBsAg with an Elisa method. The subjects were aged between 4 months and 73 years and had all suffered from acute fully recovered icteric hepatitis in the previous 2 to 10 years. All the drug addicts (13 males) and 4 healthy children from the group had no or negligible levels of antibodies; 3 out of 8 old people (60 or more yrs old) showed no antibodies only 4-5 yrs after having been infected. In the remaining group of 44 healthy people 14 cases were observed which had antibody levels 10 mUI, regardless of age, sex or occupation; the greater the time between the infection and the test, the greater the probability of finding negligible antibody levels. We conclude that the reduction in antibody levels may not be indicative of a loss of immunity: many of our cases have shown low or undetectable antibody levels, a few years after full recovery from an acute attack of hepatitis B.</p>","PeriodicalId":75613,"journal":{"name":"Bollettino dell'Istituto sieroterapico milanese","volume":"70 1-2","pages":"449-51"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12835853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Association of non-Hodgkin's lymphoma and Kaposi's sarcoma in a patient with HIV infection: case report. 非霍奇金淋巴瘤和卡波西肉瘤在HIV感染患者中的关联:病例报告。
Bollettino dell'Istituto sieroterapico milanese Pub Date : 1991-01-01
D A Verani
{"title":"Association of non-Hodgkin's lymphoma and Kaposi's sarcoma in a patient with HIV infection: case report.","authors":"D A Verani","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We describe a case of an HIV-antibody positive male intravenous drug abuser who suffered from a low grade NHL associated with Kaposi's sarcoma. Both the grade of the NHL and the Kaposi's sarcoma are relatively rare in a patient like ours as the former are usually high grade NHLs and the latter is more common in homosexuals rather than in drug addicts. Clinical and therapeutic implications of multiple pathologies in HIV-antibody positive patients are discussed.</p>","PeriodicalId":75613,"journal":{"name":"Bollettino dell'Istituto sieroterapico milanese","volume":"70 1-2","pages":"453-5"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12835854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Serological determination of anti-mumps immunity in a population sample from Palermo]. [巴勒莫人群样本抗腮腺炎免疫的血清学测定]。
Bollettino dell'Istituto sieroterapico milanese Pub Date : 1991-01-01
D Pistoia, R Di Stefano, S Arista, P Ammatuna
{"title":"[Serological determination of anti-mumps immunity in a population sample from Palermo].","authors":"D Pistoia,&nbsp;R Di Stefano,&nbsp;S Arista,&nbsp;P Ammatuna","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A seroepidemiological survey was performed by an immunoglobulin G antibody-capture immunoassay on 1061 subjects from Palermo (Sicily), throughout the age range from birth up to 50 years. A proportional increase of seropositivity rate to mumps virus was shown in relation to increasing age, from 17.5% in the earliest years up to 60.3% in children aged 10 years. After the first decade of life, seropositivity rate reached a plateau staying constant up to the upper age classes explored. Given the relatively high frequency of complications upon mumps infection, it is suggested that an extended vaccination policy could cover a proportion of the non-immune adult subjects.</p>","PeriodicalId":75613,"journal":{"name":"Bollettino dell'Istituto sieroterapico milanese","volume":"70 1-2","pages":"483-5"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12834843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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