Ai zheng = Aizheng = Chinese journal of cancer最新文献

{"title":"[Progress and prospects in cancer stem cell research for hepatocellular carcinoma].","authors":"Wen Xu,&nbsp;Lu Cao,&nbsp;Zheng-Feng Yin","doi":"10.5732/cjc.008.10835","DOIUrl":"https://doi.org/10.5732/cjc.008.10835","url":null,"abstract":"<p><p>The cancer stem cell (CSC) theory stipulates that it is a small population of cells called CSCs that initiates tumor formation and maintains its growth. CSCs are scarce within the bulk of the tumor mass, and possess stem cell-like properties such as self-renewal, differentiation and resistance to therapies, and so on. In the past few years, by using side population technique and approaches based on surface markers, including CD133, CD90, OV6 and EpCAM, researchers have identified and isolated a subpopulation of liver cancer cells with enhanced colony-forming and tumorigenic ability, which is strong evidence for the existence of liver CSCs. In this review, we summarized the progress of research on liver CSCs, discussed the significance of liver CSCs in the diagnosis and treatment of hepatocellular carcinomas, and put forward the future research directions as well as the challenges and opportunities.</p>","PeriodicalId":7559,"journal":{"name":"Ai zheng = Aizheng = Chinese journal of cancer","volume":"28 9","pages":"1004-8"},"PeriodicalIF":0.0,"publicationDate":"2009-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28382602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Measurement and analysis of the imaging dose with megavoltage computed tomography for helical tomotherapy].","authors":"Shou-Ping Xu,&nbsp;Chuan-Bin Xie,&nbsp;Zhong-Jian Ju,&nbsp;Xiang-Kun Dai,&nbsp;Han-Shun Gong,&nbsp;Yan-Yan Guo,&nbsp;Lian-Yuan Wang","doi":"10.5732/cjc.008.10632","DOIUrl":"https://doi.org/10.5732/cjc.008.10632","url":null,"abstract":"<p><strong>Background & objective: </strong>During the helical tomotherapy process, megavoltage computed tomography (MVCT) images are usually used for guiding the precise setup of patients before/after treatment delivery, which would certainly increase the total dose for patients. This study was to investigate the imaging dose of MVCT using the body and head phantom on a tomotherapy machine.</p><p><strong>Methods: </strong>A set of cylindrical body and head phantoms was adopted for scanning with different pitch values (1.0/2.0/3.0), lengths (4.8/7.2/9.6/12/14.4 cm) and patient setups on the couch of tomotherapy system. The average MVCT imaging doses were measured using A1SL chambers inserted in the phantoms with preset geometry. The dose uniformity was qualitatively analyzed.</p><p><strong>Results: </strong>The MVCT scanning dose for the body phantom was between 0.599 and 2.876 cGy during each treatment delivery, while the dose for the head phantom was between 0.913 and 3.231 cGy. Two major parameters, the assigned pitch numbers and scanning lengths, were the most important impacts to the dose variation. The MVCT dose was inversely proportional to the CT pitch value. With respect to the scanning length, the doses responded differently along the radial direction of the phantoms with different setup criteria.</p><p><strong>Conclusion: </strong>The results may provide a reliable guidance for proper planning design of the scanning region, which is valuable to help minimize the extra doses to patient.</p>","PeriodicalId":7559,"journal":{"name":"Ai zheng = Aizheng = Chinese journal of cancer","volume":" ","pages":"886-9"},"PeriodicalIF":0.0,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40020017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of topotecan on expression of aquaporin protein 5 and nuclear factor-kappaB in ovarian cancer SKOV3 cells].","authors":"Xue-Jun Chen,&nbsp;Jian-Hua Yang,&nbsp;Wei Zheng","doi":"10.5732/cjc.008.10746","DOIUrl":"https://doi.org/10.5732/cjc.008.10746","url":null,"abstract":"<p><strong>Background and objective: </strong>Overexpression of aquaporin protein 5 (AQP5) is associated with the metastasis, migration and angiogenesis of ovarian cancer, but its function in cell proliferation has not been described. This study was to explore the effects of topotecan (TPT) on the expression of AQP5, nuclear factor-kappaB (NF-kappaB) and its receptor IkappaBalpha in ovarian cancer SKOV3 cells.</p><p><strong>Methods: </strong>When SKOV3 cells were treated with TPT of various concentrations for different time, cell proliferation was measured by MTT assay, the expression of AQP5, NF-kappaB and IkappaBalpha was detected by Western blot.</p><p><strong>Results: </strong>After SKOV3 cells were incubated with 0.4 microg/mL TPT for 24 h, the expression of AQP5, NF-kappaB p65 in cytoplasm and nuclei, and IkappaBalpha in cytoplasm were decreased, and remained at low levels till 72 h (P<0.05). When SKOV3 cells were treated with 0.2, 0.4, 0.6 and 0.8 microg/mL TPT for 24 h, the expression of AQP5 as well as NF-kappaB p65 and IkappaBalpha in nuclei were decreased (P<0.005). The protein level of AQP5 was decreased by 57.9% when cells were treated with 0.8 microg/mL TPT. AQP5 expression was negatively correlated to the proliferation inhibition rate of SKOV3 cells induced by TPT (r=-0.965, P<0.05), and positively correlated to NF-kappaB p65 and IkappaBalpha expression (r=0.903, 0.896, P<0.05).</p><p><strong>Conclusion: </strong>When inhibiting the proliferation of ovarian cancer cells, TPT may down-regulate the expression of AQP5 and NF-kappaB.</p>","PeriodicalId":7559,"journal":{"name":"Ai zheng = Aizheng = Chinese journal of cancer","volume":" ","pages":"856-60"},"PeriodicalIF":0.0,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40020125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between Epstein-Barr virus infection and nasopharyngeal carcinoma pathogenesis.","authors":"Chin-Tarng Lin","doi":"10.5732/cjc.009.10107","DOIUrl":"https://doi.org/10.5732/cjc.009.10107","url":null,"abstract":"<p><p>Nasopharyngeal carcinoma (NPC) is one of the common cancers among Chinese living in South China, Taiwan, Singapore, and several other countries or regions in distinct areas. The etiological factors have not been clearly identified yet. So far, no major gene related with hereditary factor has been identified in NPC carcinogenesis; however, some environmental factors, such as consumption of salted fish and long-term exposure to sulfuric-acid vapor, have been tentatively linked to NPC induction, while research has proposed that there is a close association between Epstein-Barr virus (EBV) and NPC pathogenesis. To investigate the relationship between NPC and EBV, we have established ten NPC cell lines. After extensive investigation, we conclude that EBV may establish an infection only in nasopharyngeal neoplastic cells, not in metaplastic epithelial cells, through the IgA receptor (secretory component protein)-mediated endocytosis. Our observations indicate that EBV plays an important role in enhancement of NPC progression, but is involved in neither the initiation nor the promotion of NPC pathogenesis.</p>","PeriodicalId":7559,"journal":{"name":"Ai zheng = Aizheng = Chinese journal of cancer","volume":" ","pages":"791-804"},"PeriodicalIF":0.0,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40021234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Six anti-Epstein-Barr virus antibodies in healthy adults in a high risk area of nasopharyngeal carcinoma].","authors":"Bing Yi,&nbsp;Yao-Liang Gu,&nbsp;Yong-Sheng Zong,&nbsp;Wei-Min Cheng,&nbsp;Ming-Fang Ji","doi":"10.5732/cjc.009.10095","DOIUrl":"https://doi.org/10.5732/cjc.009.10095","url":null,"abstract":"<p><strong>Background and objective: </strong>Nasopharyngeal carcinoma (NPC), with a remarkable geographic distribution, is consistently associated with Epstein-Barr virus (EBV) infection, and almost all NPC patients have sustained high levels of serum antibodies against EBV. This study was to compare the levels of six anti-EBV antibodies in healthy natives of Zhongshan (a high-incidence area of NPC) with those in provisional migrants from foreign provinces (low-incidence areas of NPC), and to illustrate the relationship between EBV infection and the geographic distribution of NPC.</p><p><strong>Methods: </strong>The serum levels of EBNA1-IgA, EBNA1-IgG, VCA-p18-IgA, VCA-p18-IgG, Zta-IgA and Zta-IgG in 303 healthy Zhongshan natives and 92 provisional migrants were tested using ELISA, and presented by values of adjusted relative absorbance (ArA). The serum levels and positive rates of the six antibodies were compared between the two groups.</p><p><strong>Results: </strong>The mean ArA values of both Zta-IgA and VCA-p18-IgA were significantly higher in Zhongshan natives than in provisional migrants (0.84+/-0.03 vs. 0.42+/-0.04, P <0.05; 0.96+/-0.05 vs. 0.40+/-0.05, P<0.05). In addition, the positive rates of Zta-IgA and VCA-p18-IgA in subjects aged of 30-49, or of 50 and above were significantly higher in Zhongshan natives than in provisional migrants (29.27% vs. 3.03% and 48.28% vs. 6.67% for Zta-IgA, P<0.05; 28.46% vs. 9.09% and 43.10% vs. 13.33% for VCA-p18-igA, P<0.05).</p><p><strong>Conclusion: </strong>Zhongshan natives are likely to have an elevation of serum IgA antibodies against EBV lytic antigens (Zta and VCA-p18), which represents reactivation of EBV latency infection and implies that Zhongshan natives may have higher risk to develop NPC than provisional migrants.</p>","PeriodicalId":7559,"journal":{"name":"Ai zheng = Aizheng = Chinese journal of cancer","volume":" ","pages":"822-6"},"PeriodicalIF":0.0,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40021237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research advancement on relationship between Epstein-Barr virus and breast cancer].","authors":"Jian-Rong He,&nbsp;Er-Wei Song,&nbsp;Ze-Fang Ren","doi":"10.5732/cjc.009.10186","DOIUrl":"https://doi.org/10.5732/cjc.009.10186","url":null,"abstract":"<p><p>The etiology of breast cancer is still unclear. The well-known risk factors, including reproductive and other factors affecting circulating sex hormones, and genetic susceptibility, explain only about 50% of all breast cancer incidence. More and more studies have shown interest in the possibility that breast cancer may be caused by viral infection. Epstein-Barr virus (EBV) is one of the candidate viruses, but the association of EBV with breast cancer remains controversial. Here we reviewed the studies on EBV biology and the association of EBV with breast cancer, including EBV detection in breast cancer tissues, serological tests, cytologic experiments and clinical analyses, and described the limitations of current studies and future directions.</p>","PeriodicalId":7559,"journal":{"name":"Ai zheng = Aizheng = Chinese journal of cancer","volume":" ","pages":"827-30"},"PeriodicalIF":0.0,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40021238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Establishment of patient-derived xenotransplantation models for non-small cell lung cancer in immune deficient mice].","authors":"Ye-Rong Hu,&nbsp;Hong Ren,&nbsp;Zhe-Liang Liu,&nbsp;Feng-Lei Yu,&nbsp;Wen-Liang Liu,&nbsp;Xin-Min Zhou","doi":"10.5732/cjc.009.10019","DOIUrl":"https://doi.org/10.5732/cjc.009.10019","url":null,"abstract":"<p><strong>Background and objective: </strong>Targeted therapies have become a valuable therapeutic option for cancer. Establishment of different animal tumor models has become necessary. This study was to establish xenotransplantation models for patient-derived non-small cell lung cancer (NSCLC) in immune deficient mice.</p><p><strong>Methods: </strong>Immune deficient mice, BALB/C-nu, NOD/scid and SCID, 16 in each strain, were used. Sixteen tumor specimens were obtained from patients with NSCLC. Each specimen was subcutaneously transplanted into one mouse from each of the three strains. The tumor formation rate, time to tumor engraftment, tumor volume doubling time were recorded and compared among the three strains of mice. Histology of xenograft tumors was examined.</p><p><strong>Results: </strong>The total tumor formation rate was 75% (12/16). The tumor formation rate was the highest in SCID mice (56.25%). Only four tumors were engrafted in SCID mice, and two in BALB/C-nu mice. The tumor formation rate, time to tumor engraftment, and tumor volume doubling time were not significantly different among the three strains of mice. The incidence of tumor size over 1cm in the upper flanks of the mice (56.25%) was significantly higher than that in the lower flanks (25%) (P=0.037). Haematoxylin Eosin staining revealed a high degree of histological similarity between all xenograft and the parental tumors.</p><p><strong>Conclusions: </strong>We have established xenotransplantation models for patient-derived NSCLC with a success rate of 75% in BALB/C-nu and SCID mice. The xenograft tumors have the same histological features to those of their parental tumors.</p>","PeriodicalId":7559,"journal":{"name":"Ai zheng = Aizheng = Chinese journal of cancer","volume":" ","pages":"890-3"},"PeriodicalIF":0.0,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40020018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A case of myxoid chondrosarcoma arising from caput costae.","authors":"Shu-Kun Zhang,&nbsp;Jian-Guo Wang,&nbsp;Yus-Shan Piao,&nbsp;De-Hong Lu","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":7559,"journal":{"name":"Ai zheng = Aizheng = Chinese journal of cancer","volume":"28 8","pages":"894-6"},"PeriodicalIF":0.0,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28911649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of rapamycin on prostate cancer PC-3 cells].","authors":"Qian-Yuan Zhuang,&nbsp;Xian-Guo Chen,&nbsp;Zi-Qiang Dong,&nbsp;Ji-Hong Liu,&nbsp;Zhang-Qun Ye","doi":"10.5732/cjc.008.10710","DOIUrl":"https://doi.org/10.5732/cjc.008.10710","url":null,"abstract":"<p><strong>Background and objective: </strong>The mammalian target of rapamycin (mTOR) signaling network regulates cell growth, proliferation, survival and apoptosis. This study was to investigate the effect and the underlying mechanism of rapamycin on prostate cancer PC-3 cells.</p><p><strong>Methods: </strong>PC-3 cells were treated with 1 nmol/L rapamycin. The proliferation of PC-3 was examined by MTT. The cell cycle distribution of PC-3 was measured by FCM. The protein levels of raptor, rictor, Akt, pS6k1-T389, pAkt-s473 in PC-3 were examined by western blot.</p><p><strong>Results: </strong>Rapamycin increased the proliferation of PC-3 at 24 h, however, it remarkably inhibited cell proliferation after 36 h (P<0.01), which became more obviously at 72 h. Although incubation with rapamycin slightly induced cell arrest at the S phase at 24 h, this gradually increased PC-3 cells at the G1 phase at 36 h and 48 h. Compared with the control group, the protein levels of raptor and pS6k1-T389 were significantly decreased (P<0.01), and those of rictor and Akt remained unchanged after the treatment with rapamycin for 24 h; the protein level of pAkt-s473 was significantly increased at 24 h (P<0.01), but was obviously inhibited at 36 h and almost completely inhibited at 72 h (P<0.01).</p><p><strong>Conclusions: </strong>Prolonged rapamycin treatment inhibits the proliferation of PC-3 cells. This may be caused by rapamycin-induced cell cycle arrest at the G(1) phase and inhibition of Akt phosphorylation.</p>","PeriodicalId":7559,"journal":{"name":"Ai zheng = Aizheng = Chinese journal of cancer","volume":" ","pages":"851-5"},"PeriodicalIF":0.0,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40020124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Synergic effect of Na(+)-K(+) ATPaseB1 and adriamycin on inhibition of cell proliferation and reversal of drug resistance in breast cancer MCF-7 cells].","authors":"Yan-Yu Qi,&nbsp;Kai Liu,&nbsp;Jie Zhang,&nbsp;Kai Li,&nbsp;Jing-Jing Ren,&nbsp;Ping Lin","doi":"10.5732/cjc.009.10004","DOIUrl":"https://doi.org/10.5732/cjc.009.10004","url":null,"abstract":"<p><strong>Background and objective: </strong>Na(+)-K(+)ATPase (Na(+)-K(+) pump) is an important cell energy conversion system which is probably associated with tumor metastasis. Expression of its B1 subunit gene-ATP1B1 is high in well differentiated and low in poorly differentiated tumor cells. This study was to investigate the synergic effect of Na(+)-K(+) ATPaseB1 and adriamycin (ADM) on inhibition of cell proliferation and reversal of drug resistance in MCF-7 and MCF-7/ADM cells.</p><p><strong>Methods: </strong>Growth of MCF-7 and MCF-7/ADM cells transfected with PEGFP-ATP1B1 and shATP1B1 were measured by MTT. Intracellular fluorescence intensity of ADM was analyzed by inverted fluorescence microscopy and flow cytometry. ATP enzyme activity was measured by ultramicro-ATP enzyme, and mRNA expression of multi-drug resistance gene MDR1 was detected by RT-PCR and real-time PCR. The expression of P-glycoprotein (P-gp) was analyzed by western blot.</p><p><strong>Results: </strong>The sensitivity of MCF-7 and MCF-7/ADM cells transfected with pEGFP-ATP1B1 to ADM was higher in comparing to the negative control ADM-C3 (transfected with vector pEGFP-C3) and the control ADM-RPMI-1640 (cultured with RPMI-1640), and the differences between ADM-ATP1B1 and ADM-RPMI-1640 groups were statistically significant at different concentrations of adriamycin (P<0.05). After the B1 subunit was silenced, the sensitivity of cells to ADM in the ADM-shNC group was higher than that in the shATP and ADM-RPMI-1640 groups. The mean fluorescence intensity of ADM in the ADM-ATP1B1 group was higher than that in the ADM-C3 and ADM-RPMI-1640 groups (P<0.05). ATP enzyme activity was significantly higher in ADM-ATP1B1 group comparing to the ADM-RPMI-1640 group (P<0.05). mRNA expression of MDR1 gene and protein expression of P-gp were not significantly different among the ADM-ATP1B1 group and two control groups (P>0.05).</p><p><strong>Conclusion: </strong>Na(+)-K(+) ATPase B1 can synergize with ADM and reverse drug resistance to ADM in the MCF-7/ADM cell line. This may be related to ATP enzyme activity, but not to influencing the expression of MDR1 gene.</p>","PeriodicalId":7559,"journal":{"name":"Ai zheng = Aizheng = Chinese journal of cancer","volume":" ","pages":"861-7"},"PeriodicalIF":0.0,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40020126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}