Archives de l'Institut Pasteur de Tunis最新文献_第2页

MMP inhibitors and cancer treatment trials, limitations and hopes for the future. MMP抑制剂和癌症治疗的试验，局限性和对未来的希望。

Archives de l'Institut Pasteur de Tunis Pub Date : 2013-01-01

R Tlatli, M El Ayeb

{"title":"MMP inhibitors and cancer treatment trials, limitations and hopes for the future.","authors":"R Tlatli, M El Ayeb","doi":"","DOIUrl":"","url":null,"abstract":"Matrix metalloproteinases (MMPs) are a family of enzymes that have been recognized as promising therapeutic and diagnostic targets for the treatment and detection of human cancers. This rises from their unique ability to degrade all components of the extracellular matrix and their overexpression at different stages of tumor progression. The specific involvement of MMPs in the oncogenic processes has speeded up the efforts that have been made for the past 20 years to develop and evaluate MMP inhibitors (MMPIs) as potential anti-cancer agents. However, bringing an MMPI to the point of clinical approval is still a challenge. In this review, we provide an overview of the structure and function of MMPs along with their implication in cancer development. Furthermore, we focus on the literature concerning the development of broad spectrum natural and synthetic MMPIs, with emphasis on their limitations and the disappointing results of most clinical trials. The failure of broad spectrum MMPIs highlighted the need for the development of selective inhibitors that fully discriminate between different members of the MMP family. As a future perspective on the development of potent MMPIs, we also report in this review a novel structure-based strategy developed in our group to design new mini-protein ligands for MMP inhibition by functional motif grafting.","PeriodicalId":75537,"journal":{"name":"Archives de l'Institut Pasteur de Tunis","volume":"90 1-4","pages":"3-21"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33336713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coinfection with Mycobacterium tuberculosis and Pneumocystis Jirovecii in immunocompetent young woman. 免疫功能正常的年轻女性结核分枝杆菌和肺囊虫合并感染。

Archives de l'Institut Pasteur de Tunis Pub Date : 2013-01-01

I Ben-Mustapha, Kh Belkhouja, S Kheder, N Mekki, K Ben Romdhane, S Hantous, J Ben Khelil, L Saidi Slim, M Ridha Barbouche, M Besbes

引用次数: 0

[Significance and contribution to the diagnosis of multiple myeloma according to the IGF1-R gene expression profile]. 【IGF1-R基因表达谱对多发性骨髓瘤诊断的意义及贡献】。

Archives de l'Institut Pasteur de Tunis Pub Date : 2013-01-01

I Safra, I Rezgui, S Ladeb, N Skouri, A Ben Amor, A Lakhal, L Torjemane, T Ben Othman, M Ben Ahmed

{"title":"[Significance and contribution to the diagnosis of multiple myeloma according to the IGF1-R gene expression profile].","authors":"I Safra, I Rezgui, S Ladeb, N Skouri, A Ben Amor, A Lakhal, L Torjemane, T Ben Othman, M Ben Ahmed","doi":"","DOIUrl":"","url":null,"abstract":"Multiple myeloma (MM) is a still incurable adult's severe hematologic malignancy. It is characterized by deregulation of several cytokines and their receptors. Among these cytokines, Insulin growth factor 1 (IGF1) and its receptor (IGF1-R) are well documented as major factor of malignant plasma cells growth and survival in multiple myeloma. The objective of this study was to analyze the expression of IGF1-R in multiple myeloma at diagnosis in correlation with clinical and biological data. IGF1-R gene plasma cells expression was studied in 47 patients and 17 controls by Taqman technology RT-PCR. IGF1-R gene was down expressed in the malignant plasma cells of MM patients at diagnosis compared to normal plasma cells, isolated from healthy donors (p = 0.01). Expression decrease was accentuated in the disease advanced stage IIIB. A negative correlation was found between IGF1-R malignant plasma cells expression and the percentage of bone marrow invasion (p = 0.03). Bone marrow infiltration greater than 30% was significantly associated with a low level of IGF1-R gene expression (p = 0.04). Our results suggest that the decreased expression of IGF1-R by malignant plasma cells is a prognostic factor associated with severe disease. Understanding of mechanisms involved in IGF1-R expression negative regulation may contribute to the discovery of new targets therapy in myeloma. the discovery of new targets therapy in myeloma.","PeriodicalId":75537,"journal":{"name":"Archives de l'Institut Pasteur de Tunis","volume":"90 1-4","pages":"61-7"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33209656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Marine biotoxins on the Tunisian coast: current state of knowledge]. [突尼斯海岸的海洋生物毒素:目前的知识状况]。

Archives de l'Institut Pasteur de Tunis Pub Date : 2012-01-01

R Marrouchi, N Belayouni, F Dziri, R Kharrat

引用次数: 0

[Hemogram reference values: study of 1000 healthy adults from Sfax]. [血象参考值:来自Sfax的1000名健康成人的研究]。

Archives de l'Institut Pasteur de Tunis Pub Date : 2012-01-01

I Ben Amor, H Menif, A Ben Hamida, I Gdoura, H Rekik, J Gargouri

引用次数: 0

[Human immunodeficiency virus type 1 subtypes in Djibouti]. [吉布提人类免疫缺陷病毒1型亚型]。

Archives de l'Institut Pasteur de Tunis Pub Date : 2012-01-01

A Elmi Abar, A Jlizi, H Youssouf Darar, M Ben Nasr, S Abid, M Ali Ben Hadj Kacem, A Slim

{"title":"[Human immunodeficiency virus type 1 subtypes in Djibouti].","authors":"A Elmi Abar, A Jlizi, H Youssouf Darar, M Ben Nasr, S Abid, M Ali Ben Hadj Kacem, A Slim","doi":"","DOIUrl":"","url":null,"abstract":"The authors had for aim to study the distribution of HIV-1 subtypes in a cohort of HIV positive patients in the hospital General Peltier of Djibouti. An epidemiological study was made on 40 HIV-1 positive patients followed up in the Infectious Diseases Department over three months. All patients sample were subtyped by genotyping. Thirty-five patients (15 men and 20 women) were found infected by an HIV-1 strain belonging to the M group. Genotyping revealed that - 66% of samples were infected with subtype C, 20% with CRF02_AG, 8.5% with B, 2.9% with CRF02_AG/C and 2.9% with K/C. In fact, Subtype C prevalence has been described in the Horn of Africa and a similar prevalence was previously reported in Djibouti. However our study describes the subtype B in Djibouti for the first time. It is the predominant subtype in the Western world. The detection of CRF02_AG strains indicates that they are still circulating in Djibouti, the only country in East Africa in which this recombinant virus was found. CRF02_AG recombinant isolates were primarily described in West and Central Africa. The presence of this viral heterogeneity, probably coming from the mixing of populations in Djibouti, which is an essential economic and geographical crossroads, incites us to vigilance in the surveillance of this infection.","PeriodicalId":75537,"journal":{"name":"Archives de l'Institut Pasteur de Tunis","volume":"89 1-4","pages":"33-7"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32346537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The macrophage: chief of tuberculosis immune response]. 巨噬细胞:结核免疫应答的主因。

Archives de l'Institut Pasteur de Tunis Pub Date : 2012-01-01

M Haoues, M Essafi

{"title":"[The macrophage: chief of tuberculosis immune response].","authors":"M Haoues, M Essafi","doi":"","DOIUrl":"","url":null,"abstract":"With nearly eight million new cases each year and two million deaths, tuberculosis (TB) remains a major health problem worldwide. The limited protection afforded by the only available vaccine, Bacille Calmette-Guerin BCG, and the emergence of multi-resistant strains to antibiotics along with the advent of AIDS, are three main causes that contributed to the increase of TB incidence during the last decade. The World Health Organization (WHO) estimates between 2000 and 2020, nearly one billion people will be newly infected with Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, and 200 millions of them will develop the disease, of which 35 million will die if there is no improvement in controlling infection. Such improvement requires an increase in our knowledge of the fundamental biology of this very complex disease and in particular a better understanding and characterization of the types of interactions between mycobacteria and the immune system. The alveolar macrophage (MØA), the first immunological barrier that opposes the mycobacteria, plays a key role in the evolution of infection. In addition to the recognition and immediate elimination of the bacteria by phagocytosis and secretion of microbicidal products, MØA is extremely important in orchestrating the immune response and the establishment of a specific response provided by T cells. This review summarizes the state of our knowledge about the mechanisms deployed by the macrophage to contain Mtb infection with a focus on apoptosis as an innate immune response against this pathogen. We also describe the mechanisms developed by Mtb, during its coexistence with humans, in order to escape the macrophage response.","PeriodicalId":75537,"journal":{"name":"Archives de l'Institut Pasteur de Tunis","volume":"89 1-4","pages":"3-21"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32347138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Absence of viral hemorrhagic septicemia virus (VHSV) and infectious hemorrhagic necrosis virus (INHV) in a Tunisian fish farm: a case study. 在突尼斯养鱼场没有发现病毒性出血性败血症病毒(VHSV)和传染性出血性坏死病毒(INHV):一个案例研究。

Archives de l'Institut Pasteur de Tunis Pub Date : 2012-01-01

N Cherif, S Hammami

{"title":"Absence of viral hemorrhagic septicemia virus (VHSV) and infectious hemorrhagic necrosis virus (INHV) in a Tunisian fish farm: a case study.","authors":"N Cherif, S Hammami","doi":"","DOIUrl":"","url":null,"abstract":"Beyond the obvious problems related to the sustainable management of wild stocks, current fish farming practices in the Mediterranean area entail important environmental risks and potential outbreaks of fish diseases linked to massive translocations across regional boundaries. Viral haemorrhagic septicaemia (VHS) and infectious hematopoietic necrosis (IHN) are well-known fish diseases caused by the VHSV and IHNV viruses, and positive cases are subject to obligatory reporting to the World Organisation for Animal Health (OIE). In August 2004, the OIE published the first record of a VHS outbreak in a sea bass (Dicentrarchus labrax) aquaculture facility on the coast of the Mediterranean Sea. However, D. labrax is not considered as a susceptible host species for viral hemorrhagic septicaemia according to OIE's International Aquatic Animal Health Code (2009) and VHSV was not previously reported in the Mediterranean. In this sense and given the high risk of disease translocation associated with farmed fish in marine aquaculture, the present study was aimed at investigating the presence of VHSV and IHNV in stocks of sea bass and sea bream (Sparus aurata) reared inside a Tunisian coastal fish farm. Cell culture, IFAT and RT-PCR were applied to screen for both VHSV and IHNV in 69 pooled samples of sea bass and 24 pooled samples of sea bream. All three techniques showed the absence of both viruses within fish at the selected site.","PeriodicalId":75537,"journal":{"name":"Archives de l'Institut Pasteur de Tunis","volume":"89 1-4","pages":"39-45"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32346538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Ileocecal ameboma: a case diagnosed by pathology and molecular biology]. [回盲肺气肿:1例病理及分子生物学诊断]。

Archives de l'Institut Pasteur de Tunis Pub Date : 2012-01-01

E Siala, R Ben Abdallah, S Ben Ayed, K Haouet, S Haouet, O Souissi, K Aoun, A Bouratbine

引用次数: 0

Archives de l'Institut Pasteur de Tunis Pub Date : 2012-01-01

A Sassi, A Ben Salah, N Bel Haj Hamida, A Zaatour

{"title":"Age related efficiency of the leishmanin skin test as a marker of immunity to human visceral leishmaniasis.","authors":"A Sassi, A Ben Salah, N Bel Haj Hamida, A Zaatour","doi":"","DOIUrl":"","url":null,"abstract":"This study aimed at investigating whether the efficiency of the leishmanin skin test (LST) to evaluate the immune status of individuals exposed to Leishmania (L.) infantum is age-related. It was conducted in two districts of the governorate of Kairouan, an endemic region for L. infantum infection in Tunisia. Healthy individuals (n = 119) were selected according to two criteria: no current or past history of visceral or cutaneous leishmaniasis, and their age range: 1-6 years (group I), 7-14 years (group II), and 20-66 years old (group III). Assessments comprised LSTs, in vitro lymphoproliferative response, and interferon-gamma (IFN-gamma) productions induced by soluble leishmanial antigens (SLA). LST recorded an overall of 89.07% and 89.9% concordance with T cell proliferation and IFN-gamma production induced by SLA, respectively. Using in vitro tests as gold standards, LST was found more sensitive for screening individuals from group I (96% and 100%, considering T cell proliferation and IFN-gamma production results, respectively), than group II (91% and 97%) and group III (70% and 74%,). Conversely, LST was less specific in group I (84% and 77%) than group II (100% and 94%) and group III (100% for both in vitro tests). Our results suggested that the strength of LST resided in its higher sensitivity, to unravel asymptomatic injections and cell mediated immunity to L. infantum parasite in infants and its higher specificity for screening adult individuals. Negative LST in adults and positive LST in children < 5 years, the population at risk of developing visceral leishmaniasis, remain the weaknesses of LST and should be interpreted with caution.","PeriodicalId":75537,"journal":{"name":"Archives de l'Institut Pasteur de Tunis","volume":"89 1-4","pages":"23-31"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32346536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0