{"title":"Pyrolysis gas chromatography of microorganisms. Influence of various pyrolysis parameters on the yield of volatile organic products.","authors":"I Ericsson, L Larsson, P A Mårdh","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Bacteroides fragilis was analysed by pyrolysis gas chromatography (PGC). The influence of temperature rise time, pyrolysis temperature and pyrolysis time on the yield of volatile organic pyrolysis products was studied. The use of short pyrolysis time (8 msec) and a high pyrolysis end temperature (1300 degrees C) was found to provide a high yield. In consecutive analyses of the test organism, under the pyrolysis conditions indicated, the standard deviation of the total yield of volatile organic products was +/- 7%. The pyrolysis technique described suggests that few secondary reactions of the pyrolysis fragments occur.</p>","PeriodicalId":75412,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Supplement","volume":" 259","pages":"43-7"},"PeriodicalIF":0.0,"publicationDate":"1977-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11482562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cancer in Malmö 1958-1969. An autopsy study.","authors":"T Berge, S Lundberg","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75412,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Supplement","volume":" 260","pages":"1-235"},"PeriodicalIF":0.0,"publicationDate":"1977-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11482564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Diagnosis of anaerobic infections by gas-liquid chromatography.","authors":"C E Nord","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Anaerobic bacteriology of clinical specimens is an expensive and time-consuming operation. In the present investigation it was shown that fatty acids produced by anaerobic bacteria may serve as bacterial markers in clinical material. Ten specimens of pus were analyzed by gas liquid chromatography and the results were compared with the bacteriological findings. Good correlations were found for the recovery of Bacteroides fragilis and the presence of isobutyric, butyric and succinic acids. It is concluded that direct gas-liquid chromatography of clinical specimens provides a rapid presumptive test for the presence of B. fragilis in anaerobic infections.</p>","PeriodicalId":75412,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Supplement","volume":" 259","pages":"55-9"},"PeriodicalIF":0.0,"publicationDate":"1977-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11546122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pyrolysis gas chromatography -- a potential technique for characterization of complex organic material.","authors":"I Ericsson","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A pyrolysis gas chromatography (PGC) technique is described and various pyrolysers discussed. Results from analyses of synthetic polymers and organic salts are given. The influence of temperature rise time, pyrolysis temperature, pyrolysis time and sample size is considered. Pyrolysis studies on microorganisms are described various ways of improving the technique are considered.</p>","PeriodicalId":75412,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Supplement","volume":" 259","pages":"37-42"},"PeriodicalIF":0.0,"publicationDate":"1977-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11482561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pseudomonas aeruginosa infection in cystic fibrosis. Diagnostic and prognostic significance of pseudomonas aeruginosa precipitins determined by means of crossed immunoelectrophoresis. A survey.","authors":"N Hoiby","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75412,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Supplement","volume":" 262","pages":"1-96"},"PeriodicalIF":0.0,"publicationDate":"1977-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11618245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Statistical studies in the aetiology of malignant neoplasms. V. Trends and risks. Denmark 1943--72.","authors":"J Clemmesen","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75412,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Supplement","volume":" 261","pages":"1-286"},"PeriodicalIF":0.0,"publicationDate":"1977-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11486425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"High-pressure liquid chromatography as a tool for determination of antibiotics in biological fluids.","authors":"I Nilsson-Ehle","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The toxicity of some antibiotics used today necessitates rapid, specific and accurate determinations of antibiotic concentrations in biological fluids. Microbiological assays do not fulfill these needs and new procedures have recently been developed, for instance, fluorimetric assays, radioimmunoassays and radioenzymatic assays. However, these techniques have not been generally applicable to various antimicrobial agents. - The technique for high-pressure liquid chromatography (HPLC) has recently been applied to determination of antibiotics in biological fluids. The methods involve extraction of drug from the biological samples, separation by HPLC and detection by ultraviolet spectrophotometry of fluorimetry. Results obtained with tetracycline, amphotericin B, and cephalothin show that this procedure meets the demands for rapid, specific and accurate monitoring of antibiotic concentrations in body fluids. Because of its versatility, the HPLC technique seems to be applicable to the determination of a variety of antibiotics and other drugs in clinical and experimental medicine.</p>","PeriodicalId":75412,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Supplement","volume":" 259","pages":"61-6"},"PeriodicalIF":0.0,"publicationDate":"1977-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11482563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}