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Acta microbiologica Polonica. Series A: Microbiologia generalis最新文献

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A simple test for bacterial esterase on agar slants. 琼脂斜面上细菌酯酶的简单测试。
Acta microbiologica Polonica. Series A: Microbiologia generalis Pub Date : 1976-01-01
Z Józwik
{"title":"A simple test for bacterial esterase on agar slants.","authors":"Z Józwik","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75389,"journal":{"name":"Acta microbiologica Polonica. Series A: Microbiologia generalis","volume":"8 1","pages":"87-8"},"PeriodicalIF":0.0,"publicationDate":"1976-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12119721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of the concentration of phosphorus, potassium, calcium and iron compounds on the microbial reduction of sulphates. 磷、钾、钙、铁化合物浓度对硫酸盐微生物还原的影响。
Acta microbiologica Polonica. Series A: Microbiologia generalis Pub Date : 1976-01-01
F Domka, J Gasiorek
{"title":"Influence of the concentration of phosphorus, potassium, calcium and iron compounds on the microbial reduction of sulphates.","authors":"F Domka,&nbsp;J Gasiorek","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In the reduction process of sulphates by Desulfovibrio desulfuricans suitable concentration of mineral salts plays an important role. In particular, different K2HPO4, CaCl2 and Fe(NH4)2(SO4)2 content in the reaction medium has an observable effect on the reduction degree (x'), the induction time (t0) and the reduction rate constant (k). These data were recorded for 9 liquid media characterized by different content of mineral salts. The reaction rate constants were calculated for the reduction process in each medium. Optimal reduction conditions exists at the following element concentration ratios: P/S=0.068, K/S=0.086, Fe/S=0.08, N/S=0.33, C/S=1.80, Ca/S=0.03, Cl/S=0.80.</p>","PeriodicalId":75389,"journal":{"name":"Acta microbiologica Polonica. Series A: Microbiologia generalis","volume":"8 1","pages":"57-64"},"PeriodicalIF":0.0,"publicationDate":"1976-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12119720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The use of gel-filtration for the isolation of pure nisin from commercial products. 用凝胶过滤法从商业产品中分离纯化乳酸链球菌素。
Acta microbiologica Polonica. Series A: Microbiologia generalis Pub Date : 1976-01-01
A Wilimowska-Pelc, Z Olichwier, M Malicka-Blaszkiewicz, W Mejbaum-Katzenellenbogen
{"title":"The use of gel-filtration for the isolation of pure nisin from commercial products.","authors":"A Wilimowska-Pelc,&nbsp;Z Olichwier,&nbsp;M Malicka-Blaszkiewicz,&nbsp;W Mejbaum-Katzenellenbogen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Commercial nisin was fractionated using a Bio-Gel P-10 column and ion-exchange chromatography on CM-sephadex C-25. Pure nisin having a titre of 40 X 10(6) units per gram was obtained. In polyacrylamide-gel electrophoresis the pure nisin gave three bands. It is suggested that heterogeneity of nisin is due to the presence of several biological polypeptides. The pure nisin is digested by chymotrypsin but it is not affected by TPCK-trypsin and pepsin.</p>","PeriodicalId":75389,"journal":{"name":"Acta microbiologica Polonica. Series A: Microbiologia generalis","volume":"8 1","pages":"71-7"},"PeriodicalIF":0.0,"publicationDate":"1976-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12007123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of dicumarol on the growth of some soil microorganisms. 双豆酚对土壤微生物生长的影响。
Acta microbiologica Polonica. Series A: Microbiologia generalis Pub Date : 1976-01-01
B Smyk, L Van To
{"title":"Effect of dicumarol on the growth of some soil microorganisms.","authors":"B Smyk,&nbsp;L Van To","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of dicumarol on growth of selected soil bacteria: Azotobacter chroococcum, Arthrobacter globiformis, A. citreus and Bacillus megaterium was studied. The following minimum concentrations were inhibitory in vitro: Arthrobacter citreus--20 mug/ml., Bacillus megaterium--40 mug/ml., Azotobacter chroococcum--40 mug/ml. Arthrobacter globiformis--70 mug/ml. Cells of all microorganisms studied grown in the presence of dicumarol developed aberrant morphological forms.</p>","PeriodicalId":75389,"journal":{"name":"Acta microbiologica Polonica. Series A: Microbiologia generalis","volume":"8 1","pages":"65-9"},"PeriodicalIF":0.0,"publicationDate":"1976-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11281198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Growth of rubella virus in primary rabbit kidney cell cultures maintained with and without calf serum. 风疹病毒在加犊牛血清和不加犊牛血清的兔肾细胞原代培养物中的生长。
Acta microbiologica Polonica. Series A: Microbiologia generalis Pub Date : 1976-01-01
K Filczak, M Korbecki
{"title":"Growth of rubella virus in primary rabbit kidney cell cultures maintained with and without calf serum.","authors":"K Filczak,&nbsp;M Korbecki","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Rubella virus growth was tested in primary rabbit kidney cell cultures, maintained with or without calf serum. In fluid phase of the cultures maintained with 1.5% of heat-inactivated calf serum, the infectious virus titers were much higher than in the system without serum. The differences reached 1.5-2.0 log10 TCID50/ml. Virus multiplication curves also ran different courses. In serum-containing system virus titer increased up to the 9th day post infection, while without serum--only up to 4-5 days. The time of appearance and the type of cytopathic effect were not affected by the absence of serum or by its presence in a concentration of 1.5%.</p>","PeriodicalId":75389,"journal":{"name":"Acta microbiologica Polonica. Series A: Microbiologia generalis","volume":"8 1","pages":"79-86"},"PeriodicalIF":0.0,"publicationDate":"1976-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12129814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Polygalacturonase activity of Penicillum sp 7/4B/EI 1 mutant grown on some by-products of food industry. 在某些食品工业副产品上生长的青霉sp 7/4B/EI 1突变体的聚半乳糖醛酸酶活性
Acta microbiologica Polonica. Series A: Microbiologia generalis Pub Date : 1976-01-01
I Szajer
{"title":"Polygalacturonase activity of Penicillum sp 7/4B/EI 1 mutant grown on some by-products of food industry.","authors":"I Szajer","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pectolytic activity of the Penicillum sp 7/4B/E11 mutant, which synthesized effectively PG and was not capable of producing PE, was studied on some by-products of food industries (apple pulp, beet pulp, and mixtures of apple pulp or beet pulp with wheat bran) supplemented in some experiments with mineral salts of the Czapek's medium. The strain showed good growth but a low PG activity when grown on by-products without mineral salts and an increase in PG activity after cultivation on these by-products (mainly on beet pulp) enriched with mineral salts. The highest PG activity was obtained after 6-8 days cultivation on beet pulp (3%) with mineral salts. Among the tested salts, NaNO3 influenced PG activity most effectively, K2HPO4 and MgSO4 to a smaller degree whereas others were ineffective. The mutant did not synthesize PE under these culture conditions.</p>","PeriodicalId":75389,"journal":{"name":"Acta microbiologica Polonica. Series A: Microbiologia generalis","volume":"8 1","pages":"51-6"},"PeriodicalIF":0.0,"publicationDate":"1976-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12119719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Segregation of Col Ib and drd7 into minicells. colib和drd7分离成小细胞。
Acta microbiologica Polonica. Series A: Microbiologia generalis Pub Date : 1976-01-01
A Skorupska, Z Lorkiewicz
{"title":"Segregation of Col Ib and drd7 into minicells.","authors":"A Skorupska,&nbsp;Z Lorkiewicz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The wild-type plasmid ColIb and its mutant drd7 derepressed in conjugation were transferred to Escherichia coli K12 P678-54 which produces minicells. Fertility functions of drd7 remained derepressed in the new host. P678-54drd7 transmitted the plasmid at a high frequency (28.6%) and it was effectively lysed by the phage If1. Significant amounts of 3H-DNA segregated from P67854Col+ into minicells dependent upon the presence of the plasmid. The depressed plasmid segregated more effectively into minicells than the wild-type plasmid. ColIb segregated into 2% whereas ColIbdrd7 into 8.4% of minicells. The difference in the frequency of segregation of the wild-type and the derepressed plasmid indicated different cell membrane attachment sites of each plasmid studied. Mini-drd7 were able to transfer the plasmid to E. coli Row at a low frequency (0.1%). Minicells carrying either of plasmid were capable to synthesize RNA and protein. RNA and protein synthesis were plasmid specific and the precursors were not incorporated into minicells without plasmids. Rifampin and chloramphenicol inhibited RNA and protein synthesis in minicells, respectively. The more effective incorporation of 3H-uridine or 14C-leucine into minicells harboring drd7 than ColIb resulted presumably from the high efficiency of the segregation of drd7 into minicells. Polyacrylamide gel electrophoresis of 3H-RNA has shown that plasmids in minicells were able to code low molecular RNA of 4s. No 16 or 23 ribosomal RNA was found in the profiles of de novo synthesized RNA in minicells.</p>","PeriodicalId":75389,"journal":{"name":"Acta microbiologica Polonica. Series A: Microbiologia generalis","volume":"8 1","pages":"17-26"},"PeriodicalIF":0.0,"publicationDate":"1976-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11972910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transformation of Escherichia coli by R404 factor DNA and properties of the transformants. R404因子DNA对大肠杆菌的转化及其性质研究。
Acta microbiologica Polonica. Series A: Microbiologia generalis Pub Date : 1976-01-01
E Kurowska, S Cebrat, T M Lachowicz
{"title":"Transformation of Escherichia coli by R404 factor DNA and properties of the transformants.","authors":"E Kurowska,&nbsp;S Cebrat,&nbsp;T M Lachowicz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Three r-determinants (Cb Cm Cf) out of eight present in R404 factor were transferred in transformation of Escherichia coli by the plasmid DNA isolated from minicells. The r-determinants acquired in this way formed the replicon tr404-1 and were untransferable by conjugation unless another self-transmissible plasmid was present in recipient. The evidences are presented in support of hypothesis that the transfer events consist in infection of transformant cells with sex factor, recombination between tr404-1 replicon and the sex factor and finally transfer of the new formed structure to the host cells of the sex factor.</p>","PeriodicalId":75389,"journal":{"name":"Acta microbiologica Polonica. Series A: Microbiologia generalis","volume":"8 1","pages":"35-42"},"PeriodicalIF":0.0,"publicationDate":"1976-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11972911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Restoration of cytochrome c in pet46 mutant of Saccharomyces cerevisiae by Mod3 gene. 利用Mod3基因恢复酿酒酵母pet46突变体细胞色素c。
Acta microbiologica Polonica. Series A: Microbiologia generalis Pub Date : 1976-01-01
M Bień, J Kolodyński, T M Lachowicz
{"title":"Restoration of cytochrome c in pet46 mutant of Saccharomyces cerevisiae by Mod3 gene.","authors":"M Bień,&nbsp;J Kolodyński,&nbsp;T M Lachowicz","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75389,"journal":{"name":"Acta microbiologica Polonica. Series A: Microbiologia generalis","volume":"8 1","pages":"27-34"},"PeriodicalIF":0.0,"publicationDate":"1976-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11398698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of ts mutation in gene 43 of bacteriophage T4 on recombination of the bacteriophage. T4噬菌体43基因ts突变对噬菌体重组的影响
Acta microbiologica Polonica. Series A: Microbiologia generalis Pub Date : 1976-01-01
D Klimuszko, W Kunicki-Goldfinger
{"title":"Effect of ts mutation in gene 43 of bacteriophage T4 on recombination of the bacteriophage.","authors":"D Klimuszko,&nbsp;W Kunicki-Goldfinger","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Certain ts mutations in gene 43 of bacteriophage T4 are known to exhibit mutator or antimutator activities with respect to different rII mutations of this phage. The effect of these mutations on recombination frequencies of double mutant strains of phage T4 with genotype rII ts--homoallelic with respect to the ts trait--was examined. The results implicate the essential role of the genetic background in the investigated process.</p>","PeriodicalId":75389,"journal":{"name":"Acta microbiologica Polonica. Series A: Microbiologia generalis","volume":"8 1","pages":"3-16"},"PeriodicalIF":0.0,"publicationDate":"1976-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12119717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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