J Velický, I Sterzl, V Mandys, J Bednár, M Titlbach, V Niahodil
{"title":"Morphological changes in the human thyroid gland cultivated in continual flow system.","authors":"J Velický, I Sterzl, V Mandys, J Bednár, M Titlbach, V Niahodil","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In the present pilot study, the human thyroid gland tissue was cultivated under continuous culture conditions in the culture chambers of the ACUSYST-S system to determine morphological changes as well as the secretion of thyreoglobulin (Tgl) and thyroid hormones. After 24 hours of cultivation the follicular structure of the tissue was preserved in peripheral parts only, and there were regressive changes in the epithelium center. After 72 hours the regressive changes appeared in isolated follicles only; the size of the follicles increased, the height of the follicular epithelium decreased and there were macrophages present with phagocytized cell debris. After 144 hours disintegration of epithelia took place in the centre, while at the periphery the original follicles survived and very tiny new follicles formed, consisting of only 6 to 8 cells each and surrounding in section the homogeneous colloid. The parenchyma picture suggests a possibility of functional regeneration as an expression of adaptation to the conditions newly arisen during cultivation in a culture chamber. There was no significant influencing of the thyroid hormone secretion during cultivation. On the other hand, the Tgl secretion decreased throughout cultivation.</p>","PeriodicalId":75355,"journal":{"name":"Zeitschrift fur mikroskopisch-anatomische Forschung","volume":"104 5","pages":"788-96"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13246121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ultrastructural studies on the morula in cattle.","authors":"J Kuryszko, K Marcinkowski","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Embryos at morula stage were ultrastructurally examined. Blastomeres of normal embryos are characterized by the presence of ribosomes, short single and branched cisterns of endoplasmic reticulum, small spherical mitochondria and vacuolar-granular structures. An enhanced autophagy and the presence of numerous and large lipid droplets are signs of the beginning degeneration.</p>","PeriodicalId":75355,"journal":{"name":"Zeitschrift fur mikroskopisch-anatomische Forschung","volume":"104 3","pages":"521-6"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13390188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ultrastructural and immunocytochemical study on bursal follicle medulla cells in Gallus domesticus.","authors":"A Dolfi, F Giannessi, F Bianchi, M Lupetti","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The use of the monoclonal anti-cytokeratin 6 and 18 antibody Dako CK 1 revealed a marked positivity of reticulo-epithelial cells (REp). Aspecific esterase testing, light microscopy, and electron microscopy were used in order to obtain a comparison between the morphology of the lymphoid follicle medulla and the picture obtained by using the monoclonal antibody CK 1. Results showed that the bursal follicle medulla can be divided into 2 areas: an esterase-positive, cytokeratin-negative centre-medulla, and a more peripheral cytokeratin-positive, esterase-negative area. These 2 regions appear to be separated by a boundary composed of flattened REp cells. Desmosomes were also observed not only among their processes, but also between the latter and the side of the cortico-medullar boundary epithelium which is external with respect to the basal membrane.</p>","PeriodicalId":75355,"journal":{"name":"Zeitschrift fur mikroskopisch-anatomische Forschung","volume":"104 3","pages":"401-11"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13390210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F Galeotti, S Fallai, G Pagavino, P Pierleoni, P Romagnoli
{"title":"The intercellular substance of the pulp of human, fully erupted, permanent premolar teeth.","authors":"F Galeotti, S Fallai, G Pagavino, P Pierleoni, P Romagnoli","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The intercellular substance of the pulp of fully erupted, healthy, human permanent premolar teeth was studied by light microscopy. Histological and histochemical methods were applied to sections from whole, decalcified teeth and isolated, undecalcified pulps, fixed with phosphate-buffered formalin and embedded in paraplast. Fibres were mainly collagen; they formed a meshwork progressively more dense from the crown to the root apex; in the crown the fibre meshwork was denser at the periphery than in the center of the pulp. Coarse bundles of collagen fibres were found in the apical part of the pulp of about one every third teeth. Glycoproteins, recognized thanks to their P.A.S. positivity, were abundant in the basal membranes of vessels and nerve fibres and between odontoblasts, but scarce in the remaining pulp tissue. Glycosaminoglycans, recognized thanks to their alcianophilia, were more abundant in the vessel wall and in the areas with coarse bundles of collagen fibres than elsewhere in the pulp; also, they were more abundant at the periphery than in the center of the pulp. As judged by staining with alcian blue at critical electrolyte concentration, glycosaminoglycans were mostly hyaluronate, with low amounts of condroitinsulphate and dermatansulphate; some heparansulphate or keratansulphate was present only in the regions with coarse bundles of collagen fibres. The morphological and histochemical differences found among different regions of each pulp and among different pulps as well provide a basis to recognize and interpret inter-regional and inter-individual variations in the pulp response to physiological and pathological modifications affecting the hard tissues of the teeth.</p>","PeriodicalId":75355,"journal":{"name":"Zeitschrift fur mikroskopisch-anatomische Forschung","volume":"104 4","pages":"657-65"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13233115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A quantitative histological study of Golgi II neurons and pale cells in different cerebellar regions of the adult and ageing mouse brain.","authors":"R R Sturrock","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Two types of medium to large sized neurons are present in the granular layer of the mouse cerebellum. One type has a large nucleus with a prominent nucleolus and a moderate amount of cytoplasm containing Nissl substance. This type corresponds to the classical Golgi II neuron. The second type has a much smaller nucleus (mean diameter 8.4 microns) with a darkly staining nuclear envelope which is almost invariably deeply indented by cytoplasmic intrusions. The nucleolus is smaller and less conspicuous than in Golgi II neurons. These neurons are identical to the pale cells described by Altman and Bayer (1977). The numbers of both types of neuron were estimated in the spinocerebellum, lobus simplex and nodulus in mice aged 6, 15, 22, 25, 28 and 31 months. There was no significant variation in the number of either Golgi II neurons or pale cells with age in any part of the cerebellum. The number of Golgi II neurons per mm3 was similar in all parts of the cerebellum (mean 3560 mm3). This was identical to the mean number of pale cells per mm3 in the spinocerebellum and pontocerebellum but in the nodulus pale cells were much more numerous (mean 41,170 per mm3). It is postulated that pale cells are small Golgi II neurons.</p>","PeriodicalId":75355,"journal":{"name":"Zeitschrift fur mikroskopisch-anatomische Forschung","volume":"104 5","pages":"705-14"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13246246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Structure-function relationship of human spinal ligaments.","authors":"H Yahia, G Drouin, N Newman","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A combination of light, scanning and transmission electron microscopy was used to investigate the morphology and ultrastructure of normal human spinal ligaments sampled from adult surgical specimens. The ligamenta flava consist mostly of dense elastic fibers, whereas the supraspinous and interspinous ligaments are preponderantly collagenous. In all ligaments, the collagen fascicles are characterized by a regular crimp structure. The inner collagen fibers of interspinous ligaments tend to be oriented parallel to the spinous processes while those of the peripheral layers run in postero-cranial direction. The presence of proteoglycan filaments is clearly demonstrated in all of the ligaments examined. They are mainly located at the d band of the collagen fibrils. These findings are discussed in relation to the function of the posterior ligamentous system. It is suggested that the interspinous ligaments are able to transmit tension from the thoracolumbar fascia to the spine. Finally, the spinal ligaments are thought to be involved in the control mechanism of the spine.</p>","PeriodicalId":75355,"journal":{"name":"Zeitschrift fur mikroskopisch-anatomische Forschung","volume":"104 1","pages":"33-45"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13498260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Proliferative response of the mesencephalic matrix areas in the reparation of the optic tectum of Triturus cristatus carnifex.","authors":"G Minelli, P del Grande, V Franceschini, F Ciani","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The localization and proliferative response of optic tectum matrix cells has been studied in adult newt following an experimental lesion on an optic lobe. The results show that 15 days after the lesion the cells in division, autoradiographically labelled, are located in the periventricular layer. Thirty days after the lesion the labelled cells are also found in the innermost grey layers; at 90 days the injured optic tectum regains the cytoarchitecture characteristic of this centre, with labelled cells, whether in the external or in the internal pyriform layers. In all the stages the labelled cells are also found in the periventricular layers of the controlateral optic tectum, in the dorsal pallium and in the striatum. The quantitative data exhibit the existence of a direct relationship between the number of proliferating cells in the injured optic lobe and the extent of the lesion. These data show the possibility of active cellular proliferation for the reconstruction of the lesioned nervous area and for restoration of the characteristic histological structure.</p>","PeriodicalId":75355,"journal":{"name":"Zeitschrift fur mikroskopisch-anatomische Forschung","volume":"104 1","pages":"17-25"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13499772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Tectorial membrane changes in hypothyroid rats during postnatal development.","authors":"M J Pujante, M L Sala, A Angulo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The morphological changes of the tectorial membrane (TM) during the postnatal development (0, 3, 6, 12 and 25 day old) of the organ of Corti were studied by light microscopy in 20 control and hypothyroid rats. Hypothyroidism was induced by daily administration of propylthioruracil (PTU) until the end of lactation. The auditive receptor in the cochlea of the hypothyroid animals shows serious structural alterations compared with those of normal ones: abnormal persistence of Kölliker's organ, immaturity of sensory cells and supporting cells and a specific distortion of the TM. Differences with controls were first observed on the sixth postnatal day of the hypothyroid rats. The inner spiral sulcus was not shaped and the TM was attached to the Kölliker's organ. In older stages (12 and 25 days), Kölliker's organ was still present. The TM acquired a shap hump with an abnormal fibrillar arrangement in its middle part. It was still attached to the outer supporting cells by a remnant of the marginal net. It was suggested that the TM is secreted by the inner spiral limbus and Kölliker's organ. An abnormal persistence of these structures in the hypothyroidism results in a retardation of Corti's organ development. However, this conclusion does not explain the absence of the outer portion of the TM. Our study confirms the hypothesis that the secretion of any components of the marginal zone of TM is made by outer supporting cells which in PTU-treated animals appear very immature and with hypoplasia.</p>","PeriodicalId":75355,"journal":{"name":"Zeitschrift fur mikroskopisch-anatomische Forschung","volume":"104 5","pages":"729-36"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13246118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Injured mitochondria in cells of Euglena gracilis after DNA gyrase inhibitors treatment.","authors":"J Polónyi, L Ebringer, J Krajcovic, K Kapeller","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Five quinolone (ofloxacin, cinoxacin, enoxacin, ciprofloxacin, oxolinic acid) and one non-quinolone (coumermycin A1) inhibitors of prokaryotic DNA gyrase used in clinical practice for treatment of bacterial infections were experimentally examinated. As model organism the flagellate Euglena gracilis was used. Ultrastructural changes in chloroplasts and mitochondria caused by inhibitors were quantitavely evaluated. Simultaneously in all cases injury and hereditary loss of chloroplasts (bleaching) were observed in the cells. In some samples about 45% of cup-shaped mitochondria cumulated in the cytoplasm. In damaged mitochondria some degenerative signs were seen, but after the last subcultivation on drug-free media the number of injured mitochondria in the bleached cells yielded to the normal value.</p>","PeriodicalId":75355,"journal":{"name":"Zeitschrift fur mikroskopisch-anatomische Forschung","volume":"104 1","pages":"61-78"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13315673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The capsule structure of Pacinian corpuscles from the cat mesentery.","authors":"L Malinovský, L Pác, J A Vega-Alvarez, W Bozilow","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Using the electronograms of Pacinian corpuscle capsule of the cat mesentery the authors have studied in details the structure of its layers and lamellae. The thickness of the capsule lamellae ranged between 30 nm-320 nm, the mean width being 150 nm. On the surface they are overlaid by means of lamina basalis. The capsule is formed by circular layers composed, most often, by 1-2 lamellae, less frequently, by larger number of lamellae. The width of the space between the layers of lamellae is 150-820 nm. A number of lamellae is provided with longer or shorter processes. They are directed more often to the adjacent lamella with which they can be also connected by means of membrane specialization. However, they are also directed into the space between individual layers of the capsule. The importance of these processes has not been known. Furthermore, the authors have studied the reciprocal link-up of the lamellae within one layer. The lamellae are connected either side-to-side, by inserting the end of one lamella into the infolding of the other, end-to-side or end-to-end. Rich pinocytotic activity (rich occurrence of caveolae) was observed in the lamellae of the capsule. Therefore the authors are of the opinion that the capsule and its lamellae have not only mechanical significance or the function of the transfer system and filter, but they form an important component (subsystem) of the whole metabolic system in the sensory corpuscle.</p>","PeriodicalId":75355,"journal":{"name":"Zeitschrift fur mikroskopisch-anatomische Forschung","volume":"104 2","pages":"193-201"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13371472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}