Joseph Jones, Mary Jones, Charles Plate, Douglas Lewis, Michael Fendrich, Lisa Berger, Daniel Fuhrmann
{"title":"Liquid Chromatography-Tandem Mass Spectrometry Assay to Detect Ethyl Glucuronide in Human Fingernail: Comparison to Hair and Gender Differences.","authors":"Joseph Jones, Mary Jones, Charles Plate, Douglas Lewis, Michael Fendrich, Lisa Berger, Daniel Fuhrmann","doi":"10.4236/ajac.2012.31012","DOIUrl":"10.4236/ajac.2012.31012","url":null,"abstract":"<p><p>Over the past decade, the use of hair specimens for the long-term detection of the alcohol biomarker ethyl glucuronide has been increasing in popularity and usage. We evaluated the usefulness of fingernail clippings as a suitable alternative to hair for ethyl glucuronide detection. A liquid chromatography-tandem mass spectrometry method for the detection of ethyl glucuronide in fingernail clippings was fully validated and used to analyze the hair and/or fingernail specimens of 606 college-aged study participants. The limit of detection was 2 pg/mg, the limit of quantitation was 8 pg/mg and the method was linear from 8 to 2000 pg/mg. Intra- and inter-assay imprecision studies at three different concentrations (20, 40, 200 pg/mg) were all within 7.8% and all intra- and inter-assay bias studies at these levels were within 115.1% of target concentration. Ethyl glucuronide levels in fingernail (mean = 29.1 ± 55.6 pg/mg) were higher than ethyl glucuronide levels in hair (mean = 9.48 ± 22.3 pg/mg) and a correlation of the matched pairs was observed (<i>r</i> = 0.552, <i>P</i> < 0.01, <i>n</i> = 529). Evaluating each gender separately revealed that the correlation of male fingernail to male hair was large and significant (<i>r</i> = 0.782, <i>P</i> < 0.01, <i>n</i> = 195) while female hair to female fingernail was small yet significant (<i>r</i> = 0.249, <i>P</i> < 0.01, <i>n</i> = 334). The study results demonstrated that fingernail may be a suitable alternative to hair for ethyl glucuronide detection and may be the preferred sample type due to the lack of a gender bias.</p>","PeriodicalId":7477,"journal":{"name":"American Journal of Analytical Chemistry","volume":"3 1","pages":"83-91"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4847958/pdf/nihms-719959.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34446783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}