Obstetrics and gynecology research最新文献

{"title":"In Utero Alcohol and Tobacco Exposure, Maternal Depression, And Maternal Obesity Are Associated with Impaired Oligodendrocyte Differentiation in The Developing Brain.","authors":"Uday Bharai, Jamal Hamze, Benjamin Zhang, Monica Hampe, Emily Sparks, Nana Merabova, Gabriel Tatevosian, Armine Darbinyan, Mary F Morrison, Laura Goetzl, Nune Darbinian, Michael Selzer","doi":"10.26502/ogr0172","DOIUrl":"10.26502/ogr0172","url":null,"abstract":"<p><strong>Introduction: </strong>Fetal alcohol spectrum disorder (FASD) is the leading preventable cause of pediatric cognitive disability and is associated with dysmyelination. We examined possible clinical co-determinants that might interact with EtOH in impairing oligodendrocyte (OL) development. Women who drink, including pregnant women, also disproportionately suffer from depression (mDepression), which we have shown is a risk factor for FASD. Might depression during pregnancy contribute to OL pathology? Maternal obesity (mObesity) also inhibits white matter development in fetal brain. Finally, tobacco exposure inhibits not only OL development, but also the production of structural proteins, such as actin. Our human biobank derived from voluntarily terminated pregnancies allows us to study the effect of EtOH and tobacco exposure, mDepression and mObesity on OL markers.</p><p><strong>Methods: </strong>Fetal brain tissue (10 - 22 weeks) was collected and EtOH exposure estimated, based on a questionnaire adapted from the NIAAA PASS study. EtOH, tobacco, mObesity, mDepression exposed samples were compared with controls matched for gestational age and fetal gender. RNA expressions of OL markers were assayed by ddPCR. Fetal-brain-derived exosomes (FB-E) were isolated from maternal plasma. Exosomal RNA was studied for MBP, BDNF and actin mRNA expression by qRT-PCR and protein levels were confirmed by ELISA.</p><p><strong>Results: </strong>Forty-two subjects were used in EtOH, mObesity and mDepression studies, 40 cases were used in EtOH and tobacco studies, and 40 cases were used in OL-E (oligodendrocyte-derived exosomes) studies. Six cases were compared to 6 controls. EtOH exposure, mDepression and mObesity were associated with reduced mRNA expression of myelin basic <b>protein (MBP)</b>, a marker for mature OLs: ↓ 1.6-fold with EtOH, ↓ 1.5 mObesity, and ↓ 2.2 mDepression. The combination of EtOH and mObesity was associated with strong reductions in MBP expression (↓ 20.6), as was mDepression plus mObesity (↓ 2.6). No significant effects were observed for the early OL marker <b>Nkx2.2</b> (↓ 1.06). <b>Olig1</b> was reduced in single (↓ 1.85 EtOH, ↓ 1.8 mObesity) or combined groups: ↓ 5 EtOH and mDepression, ↓ 6.4 EtOH and mObesity, and ↓ 11.6 mDepression and mObesity. We observed reduced <b>Olig2</b> (↓1.1 EtOH, ↓ 29 mDepression) in all combined groups. EtOH and mDepression, and obesity were associated with much lower levels of <b>BDNF</b> (↓ 1.7 EtOH, ↓ 99). In FB-E studies, 10 cases (EtOH, Tobacco, or EtOH plus Tobacco) were compared to 10 controls: EtOH exposure, Tobacco exposure and EtOH plus Tobacco exposures were associated with reduced <b>MBP:</b> ↓ 1.8-fold by EtOH, ↓ 2.6 EtOH plus Tobacco, ↓ 1.9 Tobacco. EtOH and Tobacco had strong inhibitory effect also on <b>BDNF</b> (↓2.6), as well as on <b>Actin</b> (↓3.9). Cases with high BMI were associated with a stronger effect on MBP downregulation compared to low BMI.</p><p><strong>Conclusions: ","PeriodicalId":74336,"journal":{"name":"Obstetrics and gynecology research","volume":"8 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11887622/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143588418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prenatal Alcohol Exposure Inhibits Transient Expression of Autophagy and Synaptic Proteins in Developing Brain.","authors":"Monica Hampe, Nune Darbinian, Nana Merabova, Armine Darbinyan, Jamal Hamze, Uday Bharai, Yuri Persidsky, Mary F Morrison, Shohreh Amini, Laura Goetzl, Michael E Selzer","doi":"10.26502/ogr0173","DOIUrl":"10.26502/ogr0173","url":null,"abstract":"<p><strong>Introduction: </strong>Neuronal apoptosis and consequent inhibition of autophagy, with loss of synaptic connections are central events in the genesis of fetal alcohol spectrum disorders (FASD). However, studies of molecular mechanisms of autophagy in human fetal brain are limited. Recently, prenatal exposure to EtOH was associated with reduced miRNA-9 levels in fetal brain-derived exosomes (FB- Es) isolated from maternal plasma, which correlated with small eyes, an anatomical hallmark of fetal alcohol syndrome (FAS). Since miR-9 targets several genes that regulate synaptic plasticity, EtOH-induced inhibition of miR-9 could potentially result in dysregulation of genes involved in synaptogenesis/plasticity.</p><p><strong>Methods: </strong>Rats were fed a 6.7% EtOH liquid diet from E16 to birth. Human studies: Fetal brain tissues from elective pregnancy terminations were collected at 9-23 weeks gestational age (GA; n=20 EtOH-exposed and 20 GA- and fetal sex-matched unexposed controls). EtOH consumption was assessed by questionnaire (adapted from NIAAA PASS). Expressions of 84 genes in a synaptic plasticity microarray were assessed in human fetal brain samples, verified by qRT-PCR, and for some mRNAs, copy number was determined in FB-Es by droplet digital PCR. Protein expression was measured in brain by qWestern blot assays or with a MAP kinase microarray. Exosomal protein levels were measured by ELISA.</p><p><strong>Results: </strong>Levels of pro-apoptotic caspase-3 and Bax were significantly increased in the brains of EtOH-exposed rat pups, while early expressions of anti-apoptotic Bcl2 and biphasic Bag3 were inhibited. Phosphorylation of GSK3β was increased, and during Bag3 inhibition, the GSK3β target β-catenin also was increased. EtOH-exposed P8 and P15 rats showed motor abnormalities during low Bag3 expression. EtOH exposure reduced expression of synaptophysin and synapsin. In most synaptic plasticity pathways, levels of mRNAs were reduced. Several immediate-early genes were upregulated, but SYNPO, which is involved late in synaptic plasticity was downregulated 78%. Genes involved in Long Term Potentiation (LTP) and long-term depression (LTD) were downregulated, but the neurotoxic cytokine TNF⍺ was upregulated.</p><p><strong>Conclusions: </strong>Prenatal exposure to EtOH was associated with reduced expression of autophagy genes in the fetal brains of rats and humans. Inhibition of Bag3 correlated with upregulation of GSK3β and its downstream targets, suggesting dysregulation of β-catenin signaling. Synaptic proteins, including those implicated in LTP and LTD also were inhibited by EtOH. The results in FB-E mirrored those in brain tissue. Reduced expression of miR-9 target synaptic genes in FB-Es might serve as novel biomarkers to predict FASD.</p>","PeriodicalId":74336,"journal":{"name":"Obstetrics and gynecology research","volume":"8 1","pages":"10-24"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143627201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex-Specific Dysregulation of Placental Lipid Metabolism in Preeclampsia.","authors":"Jay S Mishra, Hanjie Zhao, Jing Zheng, Sathish Kumar","doi":"10.26502/ogr0159","DOIUrl":"10.26502/ogr0159","url":null,"abstract":"<p><strong>Background: </strong>Preeclampsia (PE) is a hypertensive disorder of pregnancy associated with adverse maternal and fetal outcomes. While placental dysfunction is implicated in PE pathogenesis, the impact of PE on placental lipid metabolism and its potential sexual dimorphism remains poorly understood.</p><p><strong>Methods: </strong>We conducted a comprehensive analysis of term placentas from PE and normotensive pregnancies with male and female fetuses. Lipid profiles were quantified using mass spectrometry, and mRNA expression of genes involved in fatty acid oxidation, esterification, and transport was assessed using qPCR.</p><p><strong>Results: </strong>Placentas from PE pregnancies exhibited elevated lipid levels, with male placentas showing a more pronounced increase in triacylglycerols, cholesteryl esters, and free cholesterol compared to female placentas. Gene expression analysis revealed sexually dimorphic alterations, with male PE placentas exhibiting upregulation of genes involved in fatty acid uptake, oxidation, and esterification, while female PE placentas showed a more complex response with both upregulation and downregulation of certain genes. Notably, peroxisomal fatty acid oxidation was upregulated in male PE placentas but suppressed in female PE placentas.</p><p><strong>Conclusions: </strong>Our findings reveal sexually dimorphic alterations in placental lipid metabolism in PE, suggesting that male placentas may be more vulnerable to lipotoxicity. These insights may have implications for understanding the pathogenesis of PE and developing sex-specific interventions to improve maternal and fetal outcomes.</p>","PeriodicalId":74336,"journal":{"name":"Obstetrics and gynecology research","volume":"7 3","pages":"49-58"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11315440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141918275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyclonal Anti-D Antibodies Significantly Reduce the Rate of Miscarriages in Rh(D) positive Women with Recurrent Pregnancy loss.","authors":"Frauke Ringel,&nbsp;Falk Lewandofski,&nbsp;Holger Kiesewetter,&nbsp;Jalid Sehouli,&nbsp;Berthold Hoppe,&nbsp;Alina Kiesewetter,&nbsp;Reinhard Hannen,&nbsp;Christian Friedrich Stoll,&nbsp;Sylvia Maas,&nbsp;Abdulgabar Salama","doi":"10.26502/ogr0119","DOIUrl":"https://doi.org/10.26502/ogr0119","url":null,"abstract":"<p><strong>Background: </strong>Macrophages play a key role in all environmental conditions surrounding pregnancy. Coating of autologous red blood cells (RBCs) with polyclonal antibodies to Rh(D) antigen may result in an immunomodulation and improved outcome in Rh(D) positive women with recurrent pregnancy loss (RPL).</p><p><strong>Methods: </strong>A total of 60 Rh(D) positive women (age 23 to 45 years) with a history of RPL and ineffective treatment with low molecular weight heparin (LMWH) and/or aspirin were included in this retrospective study. In addition to this treatment, Anti-D (300 μg) was given subcutaneously to each woman either prior to pregnancy and/or two times within 12 weeks of gestation.</p><p><strong>Results: </strong>Treatment with Anti-D in non-responders to heparin/aspirin resulted in successful pregnancies in 67% of all cases. The remaining women had only aborts (23%) or did not become pregnant (10%). None of the treated women has developed anemia due to this treatment or any other significant adverse reaction. The rate of successful pregnancies does not appear to be influenced by the administration of: Anti-D prior to pregnancy, age, thrombophilia or previous alive births.</p><p><strong>Conclusion: </strong>The improved outcome following the administration of Anti-D in women with RPL might be explained by immune modulations induced by different immune reactions including polarization of decidual macrophages. The results obtained in this study clearly indicate that Anti-D is safe and highly effective in treatment of Rh(D) positive women with RPL. However, further studies are required to support our results and to find out the optimal dose and timing of Anti-D administration.</p>","PeriodicalId":74336,"journal":{"name":"Obstetrics and gynecology research","volume":"6 1","pages":"107-114"},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10167743/pdf/nihms-1890894.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9522110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic and management dilemmas in secondary abdominal pregnancy: A case report and literature review of 314 cases from 1930-2021","authors":"Nehal Machado, A. Vasudeva, Sunanda Bharatnur, S. Kanchan","doi":"10.26502/ogr0111","DOIUrl":"https://doi.org/10.26502/ogr0111","url":null,"abstract":"","PeriodicalId":74336,"journal":{"name":"Obstetrics and gynecology research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69352004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Widest Uterine Grip Width Measurement Clinically to Predict the Success of the Vaginal Hysterectomy of a Very Large Fibroid Uterus","authors":"Ramkrishna Purohit, J. Sharma, Devajani Meher","doi":"10.26502/ogr0121","DOIUrl":"https://doi.org/10.26502/ogr0121","url":null,"abstract":"","PeriodicalId":74336,"journal":{"name":"Obstetrics and gynecology research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69352469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Literature Review Comparing Combined Oral Contraceptive Pill use and Mirena during Perimenopause","authors":"Rafia Jeddy, Ahmed S. Keshta, Kamel Ikbariah, Mohamed S. Keshta","doi":"10.26502/ogr0132","DOIUrl":"https://doi.org/10.26502/ogr0132","url":null,"abstract":"","PeriodicalId":74336,"journal":{"name":"Obstetrics and gynecology research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69352576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive Factors of Early Pregnancy Loss During In Vitro Fertilization/ Intracytoplasmic Sperm Injection (Ivf/Icsi): Retrospective Study on 1806 Embryo Transfers","authors":"Karine Morcel, P. Merviel, P. James, S. Bouée, Mathilde Le Guillou, D. Pertuisel, J. Chabaud, S. Roche, Aurore Perrin, H. Drapier, D. Beauvillard","doi":"10.26502/ogr0137","DOIUrl":"https://doi.org/10.26502/ogr0137","url":null,"abstract":"Early pregnancy loss (EPL) is a spontaneous miscarriage of a clinical pregnancy during the �rst trimester. Several factors of EPL have been studied but results were discordant. We performed a retrospective study in our ART center, comparing baseline data and IVF/ICSI outcomes between cycles with EPL, ongoing pregnancy and without pregnancy. Ectopic pregnancies and biochemical pregnancies (without visualization of a gestational sac on ultrasound) were excluded. The aim of this study is to compare these different cycles, and analyze the risk factors for EPL. We included 2555 IVF/ICSI cycles leading to 2193 oocyte pick-ups and 1806 embryo transfers. Several characteristics (women’s age, infertility diagnosis and duration, estradiol level on the day of hCG-trigger, endometrial thickness, day of embryo transfer) appeared to be risk factors of EPL in univariate analysis. Only women’s age has a signi�cant (p < 0.001) in�uence in multivariate analysis on the rate of EPL, with an OR: 1.71 if the woman’s age ≥ 35 years old (reference < 35 y.o = 1), 2.96 if ≥ 38 y.o and 5.31 if ≥ 40 y.o. In this study, we observed an increase in EPL rate by 4.15% per year in women over 35 years of age.","PeriodicalId":74336,"journal":{"name":"Obstetrics and gynecology research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69352611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Probable Generalized Anxiety Disorder with The Planning or Acceptance of The Pregnancy From The Beginning in Women Undergoing Prenatal Care","authors":"Monterrosa-Castro A, F. J, Romero-Martínez G, Romero-Martínez S","doi":"10.26502/ogr0115","DOIUrl":"https://doi.org/10.26502/ogr0115","url":null,"abstract":"","PeriodicalId":74336,"journal":{"name":"Obstetrics and gynecology research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69352053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge of Pregnant Women in Rural Mozambique on Routine Practices to Prevent and Treat Common Conditions at the Antenatal Care Clinic","authors":"T. Nhampossa, K. Munguambe, Célia Chaúque, Mariza Chivangue, Maura Mazuze, Anete Mendes, L. García-Otero, R. González, Esperança Sevene, C. Menéndez, N. Torres","doi":"10.26502/ogr0117","DOIUrl":"https://doi.org/10.26502/ogr0117","url":null,"abstract":"","PeriodicalId":74336,"journal":{"name":"Obstetrics and gynecology research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69352125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}