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Quality control for Adeno-associated viral vector production. 腺相关病毒载体生产的质量控制。
Neuromethods Pub Date : 2023-01-01 Epub Date: 2023-02-07 DOI: 10.1007/978-1-0716-2918-5_5
Ina Ersing, Meghan Rego, Chen Wang, Yijun Zhang, Kate Harten DeMaio, Michelle Tillgren, Alanna Fava, Gabrielle Clouse, Marcella Patrick, Karen Guerin, Melina Fan
{"title":"Quality control for Adeno-associated viral vector production.","authors":"Ina Ersing, Meghan Rego, Chen Wang, Yijun Zhang, Kate Harten DeMaio, Michelle Tillgren, Alanna Fava, Gabrielle Clouse, Marcella Patrick, Karen Guerin, Melina Fan","doi":"10.1007/978-1-0716-2918-5_5","DOIUrl":"https://doi.org/10.1007/978-1-0716-2918-5_5","url":null,"abstract":"<p><p>Adeno-associated viral vectors (AAV) are frequently used by neuroscientists to deliver tools, such as biosensors and optogenetic and chemogenetic actuators, <i>in vivo</i>. Despite its widespread use, AAV vector characterization and quality control can vary between labs and viral vector cores leading to variable results and irreproducibility. This protocol describes some of the characterization and quality control assays necessary to confirm an AAV vector's titer, genomic identity, serotype and purity.</p>","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"195 ","pages":"77-101"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10997381/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140874130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigating Cerebrocerebellar Neuronal Interactions in Freely Moving Using Multi-electrode, Multi-site 利用多电极、多位点研究自由运动中的脑小脑神经元相互作用
Neuromethods Pub Date : 2022-01-01 DOI: 10.1007/978-1-0716-2026-7_11
Yu Liu, Brittany L. Correia, Mia B. Fox, D. Heck
{"title":"Investigating Cerebrocerebellar Neuronal Interactions in Freely Moving Using Multi-electrode, Multi-site","authors":"Yu Liu, Brittany L. Correia, Mia B. Fox, D. Heck","doi":"10.1007/978-1-0716-2026-7_11","DOIUrl":"https://doi.org/10.1007/978-1-0716-2026-7_11","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51711732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In Vitro Voltage of Subthreshold Activity in Neurons with ANNINE-6plus 含annine -6 +神经元阈下活性的体外电压
Neuromethods Pub Date : 2022-01-01 DOI: 10.1007/978-1-0716-2026-7_8
K. Dorgans, B. Kuhn, M. Uusisaari
{"title":"In Vitro Voltage of Subthreshold Activity in Neurons with ANNINE-6plus","authors":"K. Dorgans, B. Kuhn, M. Uusisaari","doi":"10.1007/978-1-0716-2026-7_8","DOIUrl":"https://doi.org/10.1007/978-1-0716-2026-7_8","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51712288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Role of Hypothalamic-Pituitary-Adrenal Axis in the Pathophysiology of Major Depression: A Translational Research Perspective of the Prednisolone Suppression Test 下丘脑-垂体-肾上腺轴在重度抑郁症病理生理中的作用:强的松龙抑制试验的转化研究视角
Neuromethods Pub Date : 2022-01-01 DOI: 10.1007/978-1-0716-2083-0_1
Mario F. Juruena, A. Cleare, A. H. Young
{"title":"The Role of Hypothalamic-Pituitary-Adrenal Axis in the Pathophysiology of Major Depression: A Translational Research Perspective of the Prednisolone Suppression Test","authors":"Mario F. Juruena, A. Cleare, A. H. Young","doi":"10.1007/978-1-0716-2083-0_1","DOIUrl":"https://doi.org/10.1007/978-1-0716-2083-0_1","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51712525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Strategies for Identification of Medial Olivocochlear Neurons for Patch-Clamp Studies of Synaptic Function Using Electrical Stimulation and Optogenetics 利用电刺激和光遗传学技术进行突触功能膜片钳研究的内耳蜗神经元鉴定策略
Neuromethods Pub Date : 2022-01-01 DOI: 10.1007/978-1-0716-2022-9_15
Kirupa Suthakar, Lester Torres Cadenas, C. Weisz
{"title":"Strategies for Identification of Medial Olivocochlear Neurons for Patch-Clamp Studies of Synaptic Function Using Electrical Stimulation and Optogenetics","authors":"Kirupa Suthakar, Lester Torres Cadenas, C. Weisz","doi":"10.1007/978-1-0716-2022-9_15","DOIUrl":"https://doi.org/10.1007/978-1-0716-2022-9_15","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51711305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optogenetics in Complex Model Systems (Non-Human Primate) 复杂模型系统中的光遗传学(非人灵长类)
Neuromethods Pub Date : 2022-01-01 DOI: 10.1007/978-1-0716-2026-7_15
Robijanto Soetedjo, Y. Kojima
{"title":"Optogenetics in Complex Model Systems (Non-Human Primate)","authors":"Robijanto Soetedjo, Y. Kojima","doi":"10.1007/978-1-0716-2026-7_15","DOIUrl":"https://doi.org/10.1007/978-1-0716-2026-7_15","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51711854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
The Role of Transcriptional Profiling in Neurobiology and Treatment of Major Depression: A Translational Perspective on RNA Sequencing Platform 转录谱分析在神经生物学和重度抑郁症治疗中的作用:RNA测序平台的翻译视角
Neuromethods Pub Date : 2022-01-01 DOI: 10.1007/978-1-0716-2083-0_10
B. Roy, P. Korla, Yogesh K. Dwivedi
{"title":"The Role of Transcriptional Profiling in Neurobiology and Treatment of Major Depression: A Translational Perspective on RNA Sequencing Platform","authors":"B. Roy, P. Korla, Yogesh K. Dwivedi","doi":"10.1007/978-1-0716-2083-0_10","DOIUrl":"https://doi.org/10.1007/978-1-0716-2083-0_10","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51712729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Serotonin Receptors and Antidepressants: Neuroimaging Findings from Preclinical and Clinical Research 血清素受体和抗抑郁药:临床前和临床研究的神经影像学发现
Neuromethods Pub Date : 2022-01-01 DOI: 10.1007/978-1-0716-2083-0_18
P. Handschuh, M. Konadu, B. Spurny-Dworak, L. Silberbauer, M. Murgaš, R. Lanzenberger
{"title":"Serotonin Receptors and Antidepressants: Neuroimaging Findings from Preclinical and Clinical Research","authors":"P. Handschuh, M. Konadu, B. Spurny-Dworak, L. Silberbauer, M. Murgaš, R. Lanzenberger","doi":"10.1007/978-1-0716-2083-0_18","DOIUrl":"https://doi.org/10.1007/978-1-0716-2083-0_18","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51713247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Ketamine and Other Glutamate Receptor Antagonists As Fast-Actin 氯胺酮和其他谷氨酸受体拮抗剂如快速肌动蛋白
Neuromethods Pub Date : 2022-01-01 DOI: 10.1007/978-1-0716-2083-0_19
Mu-Hong Chen, T. Su, S. Tsai
{"title":"Ketamine and Other Glutamate Receptor Antagonists As Fast-Actin","authors":"Mu-Hong Chen, T. Su, S. Tsai","doi":"10.1007/978-1-0716-2083-0_19","DOIUrl":"https://doi.org/10.1007/978-1-0716-2083-0_19","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51713308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Single-Cell Mass Spectrometry of Metabolites and Proteins for Systems and Functional Biology. 系统和功能生物学中代谢物和蛋白质的单细胞质谱分析。
Neuromethods Pub Date : 2022-01-01 DOI: 10.1007/978-1-0716-2525-5_5
Erika P Portero, Leena Pade, Jie Li, Sam B Choi, Peter Nemes
{"title":"Single-Cell Mass Spectrometry of Metabolites and Proteins for Systems and Functional Biology.","authors":"Erika P Portero,&nbsp;Leena Pade,&nbsp;Jie Li,&nbsp;Sam B Choi,&nbsp;Peter Nemes","doi":"10.1007/978-1-0716-2525-5_5","DOIUrl":"https://doi.org/10.1007/978-1-0716-2525-5_5","url":null,"abstract":"<p><p>Molecular composition is intricately intertwined with cellular function, and elucidation of this relationship is essential for understanding life processes and developing next-generational therapeutics. Technological innovations in capillary electrophoresis (CE) and liquid chromatography (LC) mass spectrometry (MS) provide previously unavailable insights into cellular biochemistry by allowing for the unbiased detection and quantification of molecules with high specificity. This chapter presents our validated protocols integrating ultrasensitive MS with classical tools of cell, developmental, and neurobiology to assess the biological function of important biomolecules. We use CE and LC MS to measure hundreds of metabolites and thousands of proteins in single cells or limited populations of tissues in chordate embryos and mammalian neurons, revealing molecular heterogeneity between identified cells. By pairing microinjection and optical microscopy, we demonstrate cell lineage tracing and testing the roles the dysregulated molecules play in the formation and maintenance of cell heterogeneity and tissue specification in frog embryos (<i>Xenopus laevis</i>). Electrophysiology extends our workflows to characterizing neuronal activity in sections of mammalian brain tissues. The information obtained from these studies mutually strengthen chemistry and biology and highlight the importance of interdisciplinary research to advance basic knowledge and translational applications forward.</p>","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"184 ","pages":"87-114"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9872963/pdf/nihms-1864086.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10030291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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