Neuromethods最新文献

RAPID Autofocusing in Light-Sheet Microscopy: An Implementation Guide 在薄片显微镜快速自动聚焦：实施指南

Neuromethods Pub Date : 2026-01-01 DOI: 10.1007/978-1-0716-5100-1_7

Ludovico Silvestri

引用次数: 0

Future Directions of Esketamine Nasal Spray Continuation in Treatment-Resistant Depression 艾氯胺酮鼻喷雾剂在难治性抑郁症治疗中的应用前景

Neuromethods Pub Date : 2025-01-01 DOI: 10.1007/978-1-0716-4599-4_18

Maxwell Zachary Price, Richard L. Price

引用次数: 0

Identification of Neuronal Ensembles from Similarity Maps Using Singular Value Decomposition 基于奇异值分解的相似映射神经元集成识别

Neuromethods Pub Date : 2024-11-26 DOI: 10.1007/978-1-0716-4208-5_5

Ricardo Velazquez-Contreras, Luis Carrillo‐Reid

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引用次数: 0

High-Density Multichannel Fiber Photometry 高密度多通道光纤测光

Neuromethods Pub Date : 2024-10-07 DOI: 10.1007/978-1-0716-4120-0_5

Yaroslav Sych, Fritjof Helmchen

引用次数: 0

A STED Microscope for Multimodal Investigation of Dendritic Spine Structure and Function 树突棘结构与功能多模态研究的STED显微镜

Neuromethods Pub Date : 2024-01-01 DOI: 10.1007/978-1-0716-4011-1_13

Federica Quici, Yulia Dembitskaya, Stéphane Bancelin, Jan Tønnesen, U. Valentin Nägerl

引用次数: 1

Integrated Approach for the Differentiation of Neurons and Astrocytes from Human Pluripotent Stem Cells 人类多能干细胞分化神经元和星形胶质细胞的综合方法

Neuromethods Pub Date : 2024-01-01 DOI: 10.1007/978-1-0716-3999-3_17

Yu Tang, Junjiao Wu

引用次数: 0

Gene editing and reprogramming of human fibroblast cells (hFBs) to human pluripotent stem cells (hiPSCs). 人成纤维细胞（hFBs）到人多能干细胞（hiPSCs）的基因编辑和重编程。

Neuromethods Pub Date : 2024-01-01 Epub Date: 2024-07-14 DOI: 10.1007/978-1-0716-3999-3_4

Zhe Zhang, Shinghua Ding

{"title":"Gene editing and reprogramming of human fibroblast cells (hFBs) to human pluripotent stem cells (hiPSCs).","authors":"Zhe Zhang, Shinghua Ding","doi":"10.1007/978-1-0716-3999-3_4","DOIUrl":"10.1007/978-1-0716-3999-3_4","url":null,"abstract":"<p><p>Predictive disease models play significant roles in advancing our knowledge of the pathology of human disease. In this field, animal models have been extensively employed and have provided crucial insights into the pathophysiological mechanisms of human disease. However, they often fail to fully capture many human phenotypes due to significant species differences in genomic responses. Human induced pluripotent stem cells (hiPSCs) are genetically reprogrammed cells that exhibit qualities remarkably similar to those of embryonic stem cells (ESC) and have emerged as a promising source for cell therapy and fundamental research in pathology. The ability to reprogram human fibroblast cells (hFBs) to iPSCs provides an opportunity to model human diseases. However, even hiPSCs from different persons have different genetic background, thus generation of isogenic unaffected control hiPSCs is necessary to study model human disease. Here, we describe methods to generate isogenic hFBs using CRISPR/Cas9 gene editing method, and subsequently reprogram them into iPSCs using commercially available Sendai virus vectors. Specifically, using the CRISPR/Cas9 system and Sendai virus vector, isogenic iPSC lines can be generated. This protocol provides a structured approach for obtaining multiple isogeneic hiPSC lines, which facilitate the modeling of various human diseases.</p>","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"210 ","pages":"39-59"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12707990/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145776463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation and Characterization of Human Induced Pluripotent Stem Cells from Urine-Derived Cells 人尿源性细胞诱导多能干细胞的生成与表征

Neuromethods Pub Date : 2024-01-01 DOI: 10.1007/978-1-0716-3999-3_3

Zhiqing Hu, Miaojin Zhou

引用次数: 0

Mapping Synaptic Inputs to Oligodendroglial Cells Using In Vivo Monosynaptic Viral Tracing 利用体内单突触病毒追踪将突触输入映射到少突胶质细胞

Neuromethods Pub Date : 2024-01-01 DOI: 10.1007/978-1-0716-3742-5_14

Belgin Yalçın, Michelle Monje

引用次数: 0

Generation of Human Induced Pluripotent Stem Cells Using Episomal Vectors 利用Episomal载体制备人诱导多能干细胞

Neuromethods Pub Date : 2024-01-01 DOI: 10.1007/978-1-0716-3999-3_2

S. Zhang, Yu Tang, Lu Shen

引用次数: 0