Julio F Marchini, Ayumi Aurea Miyakawa, Flavio Tarasoutchi, José Eduardo Krieger, Pedro Lemos, Kevin Croce
{"title":"Endothelial, platelet, and macrophage microparticle levels do not change acutely following transcatheter aortic valve replacement.","authors":"Julio F Marchini, Ayumi Aurea Miyakawa, Flavio Tarasoutchi, José Eduardo Krieger, Pedro Lemos, Kevin Croce","doi":"10.1186/s12952-016-0051-2","DOIUrl":"https://doi.org/10.1186/s12952-016-0051-2","url":null,"abstract":"<p><strong>Background: </strong>Patients with severe aortic stenosis have increased levels of prothrombotic and proinflammatory microparticles (MP), and MPs actively regulate pathological processes that lead to atherothrombotic cardiovascular events. Shear stress is a validated stimulus of MP production, and abnormal shear stress in aortic stenosis increases MP release in ex-vivo studies. We hypothesized that in patients with severe aortic stenosis, percutaneous replacement of the aortic valve (TAVR) would reduce abnormal shear stress and would decrease levels of circulating MPs.</p><p><strong>Findings: </strong>The experimental protocol utilized flow cytometry (FC) and nanoparticle tracking analysis (NTA) to quantify circulating plasma MP levels in aortic stenosis patients at baseline and 5 days after TAVR. The baseline and 5 day MP counts measured by FC were 6.10⋅10(5) ± 1.21⋅10(5) MP/μL and 5.74⋅10(5) ± 9.54⋅10(4) MP/μL, respectively (p = 0.91). The baseline and 5 day MP counts measured by NTA were 9.29⋅10(13) ± 1.66⋅10(13) MP/μL and 3.95⋅10(14) ± 3.11⋅10(14) MP/μL, respectively (p = 0.91). When MPs were stratified by cell source, there was no difference in pre/post TAVR endothelial, platelet, or leukocyte MP levels.</p><p><strong>Conclusion: </strong>Levels of circulating MPs do not change acutely following TAVR therapy for aortic stenosis. Trial registered at clinicaltrials.gov NCT02193035 on July 11, 2014.</p>","PeriodicalId":73849,"journal":{"name":"Journal of negative results in biomedicine","volume":"15 ","pages":"7"},"PeriodicalIF":0.0,"publicationDate":"2016-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12952-016-0051-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34447421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Targeted drugs and Psycho-oncological intervention for breast cancer patients","authors":"F. D’Abramo, U. Goerling, C. Guastadisegni","doi":"10.1186/s12952-016-0049-9","DOIUrl":"https://doi.org/10.1186/s12952-016-0049-9","url":null,"abstract":"","PeriodicalId":73849,"journal":{"name":"Journal of negative results in biomedicine","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12952-016-0049-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65694378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kota Sato, K. Nishiguchi, K. Maruyama, Satoru Moritoh, Kosuke Fujita, Yoshikazu Ikuta, H. Kasai, T. Nakazawa
{"title":"Topical ocular dexamethasone decreases intraocular pressure and body weight in rats","authors":"Kota Sato, K. Nishiguchi, K. Maruyama, Satoru Moritoh, Kosuke Fujita, Yoshikazu Ikuta, H. Kasai, T. Nakazawa","doi":"10.1186/s12952-016-0048-x","DOIUrl":"https://doi.org/10.1186/s12952-016-0048-x","url":null,"abstract":"","PeriodicalId":73849,"journal":{"name":"Journal of negative results in biomedicine","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12952-016-0048-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65694364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mariana Herrera, Yudy Aguilar, Z. Rueda, C. Muskus, L. Vélez
{"title":"Comparison of serological methods with PCR-based methods for the diagnosis of community-acquired pneumonia caused by atypical bacteria","authors":"Mariana Herrera, Yudy Aguilar, Z. Rueda, C. Muskus, L. Vélez","doi":"10.1186/s12952-016-0047-y","DOIUrl":"https://doi.org/10.1186/s12952-016-0047-y","url":null,"abstract":"","PeriodicalId":73849,"journal":{"name":"Journal of negative results in biomedicine","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12952-016-0047-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65694351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Thanks to all those who reviewed for Journal of Negative Results in BioMedicine in 2015","authors":"B. Olsen, D. Mielenz","doi":"10.1186/s12952-016-0046-z","DOIUrl":"https://doi.org/10.1186/s12952-016-0046-z","url":null,"abstract":"","PeriodicalId":73849,"journal":{"name":"Journal of negative results in biomedicine","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12952-016-0046-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65694318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ADAMTS-18 in the host tissues exerts little effect on breast tumor progress in a murine 4T1 breast cancer model","authors":"Min Liu, T. Lu, Fang Jing, S. Dang, Wei Zhang","doi":"10.1186/s12952-016-0045-0","DOIUrl":"https://doi.org/10.1186/s12952-016-0045-0","url":null,"abstract":"","PeriodicalId":73849,"journal":{"name":"Journal of negative results in biomedicine","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12952-016-0045-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65694307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Haider, G. Lindner, M. Wolzt, A. Leichtle, G. Fiedler, T. Sauter, V. Fuhrmann, A. Exadaktylos
{"title":"Use of Diuretics is not associated with mortality in patients admitted to the emergency department: results from a cross-sectional study","authors":"D. Haider, G. Lindner, M. Wolzt, A. Leichtle, G. Fiedler, T. Sauter, V. Fuhrmann, A. Exadaktylos","doi":"10.1186/s12952-016-0044-1","DOIUrl":"https://doi.org/10.1186/s12952-016-0044-1","url":null,"abstract":"","PeriodicalId":73849,"journal":{"name":"Journal of negative results in biomedicine","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12952-016-0044-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65694294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mobile phone use and risk for intracranial tumors","authors":"G. Alexiou, C. Sioka","doi":"10.1186/s12952-015-0043-7","DOIUrl":"https://doi.org/10.1186/s12952-015-0043-7","url":null,"abstract":"","PeriodicalId":73849,"journal":{"name":"Journal of negative results in biomedicine","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12952-015-0043-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65694744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Substrate stiffness effect and chromosome missegregation in hIPS cells","authors":"S. Acevedo-Acevedo, W. Crone","doi":"10.1186/s12952-015-0042-8","DOIUrl":"https://doi.org/10.1186/s12952-015-0042-8","url":null,"abstract":"","PeriodicalId":73849,"journal":{"name":"Journal of negative results in biomedicine","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12952-015-0042-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65694732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Krantz, E. Havranek, R. Pereira, B. Beaty, P. Mehler, C. Long
{"title":"Effects of omega-3 fatty acids on arterial stiffness in patients with hypertension: a randomized pilot study","authors":"M. Krantz, E. Havranek, R. Pereira, B. Beaty, P. Mehler, C. Long","doi":"10.1186/s12952-015-0040-x","DOIUrl":"https://doi.org/10.1186/s12952-015-0040-x","url":null,"abstract":"","PeriodicalId":73849,"journal":{"name":"Journal of negative results in biomedicine","volume":"14 9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12952-015-0040-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65694679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}