{"title":"A Study on the Neurotoxicity of Flunitrazepam (Rohypnol) Administration on the Cerebral Cortex of Adult Wistar Rats","authors":"Udodi Princewill Sopuluchukwu, Ezejindu Damian Nnabuihe","doi":"10.13189/APP.2021.090202","DOIUrl":"https://doi.org/10.13189/APP.2021.090202","url":null,"abstract":"Flunitrazepam (Rohypnol) is a benzodiazepine drug medically proscribed in United States whereas it’s legally prescribed in over sixty nations where the drug is administered as pre-anesthetic drug and in the treatment of insomnia but not without the loss of cerebral cortex functions which inspired this investigation to establish its neurotoxicity on the cerebral cortex of adult wistar rat. Sixteen adult female wistar rats were randomly divided into four groups with four rats in each group (A, B, C and D). Group A, the control group received distilled water. Groups B, C and D received 1mg/kg, 2mg/kg and 3mg/kg of Flunitrazepam (Rohypnol) respectively. The animals were sacrificed after three weeks of administration and the cerebral cortexes were harvested and fixed in 10% formal saline for histological processing and studies. The anthropometric investigation presented an insignificant increase in the relative body weight of the test groups when compared to the control group and also presented a significant increase in the relative organ weight in the entire test groups when compared to the control group. The cerebral cortex of experimental animals showed; severe lymphocytic infiltration, focal areas of liquefactive necrosis, intracerebral hemorrhage, vacuolation and various degrees of damages to the tissue layers and neuronal cells of the cerebral cortex. This scientific investigation indicates that consumption of Flunitrazepam (Rohypnol) across various graded doses causes changes in the cerebral cortex histoarchitecture which explains the rationale behind the loss of cerebral cortex function in drug addicts and patients placed on prolonged Flunitrazepam (Rohypnol) therapy.","PeriodicalId":7378,"journal":{"name":"Advances in Pharmacology and Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84667358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phytochemical Analysis, In Vitro Evaluation of Antioxidant and Free Radical Scavenging Activity of Simarouba glauca Seeds","authors":"R. Haleshappa, Sharangouda J. Patil, K. Murthy","doi":"10.13189/APP.2021.090101","DOIUrl":"https://doi.org/10.13189/APP.2021.090101","url":null,"abstract":"Traditional Indian medicinal plant Simarouba glauca is a highly sacred plant known as “Paradise Tree or Lakshmi Taru” which is recognized for its pharmacological and pharmaceutical properties. The present study was envisaged to know the in vitro phytochemical, antioxidant and free radical scavenging activity of S. glauca seeds. Petroleum ether was used as a solvent to extract plant material using hot extraction method. In our investigation, phytochemical analysis was carried out qualitatively and quantitatively and for the evaluation of antioxidant profile carried out in vitro studies on free radical scavenging potential by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging, decolourization potential of 2,2’-azino-bis[3-ethyl benzthiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant potential (FRAP) assay were determined in in vitro studies. The qualitative phytochemical analysis of this plant was positive for flavonoids, fatty acids, proteins, steroids and terpenoids and in quantitative analysis total flavonoid content was 17.32±0.12 mg/μg quercetin equivalent, total proanthocyanidin content exhibited 62.91±0.61 µg catechin equivalent, total phenol content exhibited 17.75±5.82 μg gallic acid equivalent and the total flavonol content was 1.54±0.01 µg quercetin equivalent of the extract. In the antioxidant profile, the maximum DPPH scavenging activity exhibited was 74% with an IC 50 value 137.89 µg/mL, the maximum ABTS scavenging activity exhibited was 35% with an IC 50 value 337.29 µg/mL and FRAP scavenging activity exhibited 62.5 µg/mL as ascorbic acid equivalents (AAE/ml) concentration. These findings evidenced that, the petroleum ether extract of S. glauca seeds has potential source of natural antioxidants and can be used it as therapeutic medicine.","PeriodicalId":7378,"journal":{"name":"Advances in Pharmacology and Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80154867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Iqra Iftikhar, A. Khalid, Zainab Bibi, A. Mehmood, Muhammad Rizwan, Sajid Khan, Anum Munir
{"title":"Computational Prediction of Tumor-Specific Antigens as Potential Vaccine Candidates against Germ-line Mutations in Endometrial Cancer","authors":"Iqra Iftikhar, A. Khalid, Zainab Bibi, A. Mehmood, Muhammad Rizwan, Sajid Khan, Anum Munir","doi":"10.13189/APP.2019.070401","DOIUrl":"https://doi.org/10.13189/APP.2019.070401","url":null,"abstract":"Endometrial cancer is the fourth most common cancer in women. It arises from the endometrium and accompanied by the abnormal growth of the cells. Sign and symptoms include pelvic pain and abnormal vaginal bleeding. It has two categories. Type 1 tumors are estrogen-dependent and they have mutations in PTEN, PIK3CA while Type 2 tumors are more sensitive and have mutations in TP53. Overactivation of the signaling pathway (PI3K) results in anti-apoptosis. Here, this study aims to identify Tumor-Specific Antigen for germline mutations in endometrial cancer which can be used as a potential vaccine candidate. The germline mutations data are obtained from cancer gene census of the cosmic database. Genes mutating with crucial role in endometrial cancer are considered. Peptides libraries are generated using peptide design library. Human leukocyte antigen alleles are identified for the peptide library through NetMHC. Binding affinities of alleles with peptide are determined. Linear regression is performed to generate graphs. PTEN, TP53, PIK3CA, KRAS, and CTNNB1 proved to have critical role. About 575 overlapping peptide libraries are generated and each peptide has a length of 18-20 amino acids. Approximately 58 HLAs are identified, having strong interactions with HLAs. Regression analysis shows that the no. of mutations are directly associated with a binding affinity of peptides. From this, we suggest that the identified TSA can be used as personalized peptide vaccines that directly target the mutated genes in endometrial cancer. This research work can be used in the laboratories for further validation.","PeriodicalId":7378,"journal":{"name":"Advances in Pharmacology and Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72953442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Hamza, Azhar Mahmood, Sajid Khan, Muhammad Rizwan, Anum Munir
{"title":"Design and Synthesis of Novel Inhibitor against Ser121 and Val122 Mutations in P53 Cancer Gene","authors":"M. Hamza, Azhar Mahmood, Sajid Khan, Muhammad Rizwan, Anum Munir","doi":"10.13189/APP.2019.070402","DOIUrl":"https://doi.org/10.13189/APP.2019.070402","url":null,"abstract":"The p53 is also known as a tumor suppressor gene, involved in a variety of cellular processes and signaling pathways. p53 mutations are involved in almost all kinds of cancers, and several treatments are available for p53 mutations but have a number of limitations. Still, there is a need for better drugs. Computational methods are emerging and beneficial tools to guide and interpret experiments to fasten the drug design process. This study was undertaken to design a drug that targets p53 Ser121 and Val122 mutations. The compound was identified through virtual screening and several drug-like filters were applied. The identified compound is considered to be non-toxic in nature. ADMET properties and pharmacokinetics of the compound also describe the effectiveness of the compound. The results of this study, suggest that this compound can be used to treat p53 mutations and the compound is synthesized successfully in the lab to determine its adequacy and efficacy. Bis-(4-chlorophenyl)methyl-BLAH compound can be used as a strong inhibitor of p53 Ser 121 and Val 122 mutations.","PeriodicalId":7378,"journal":{"name":"Advances in Pharmacology and Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76255454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Turk, M. Hamza, Anum Munir, Sajid Khan, Muhammad Rizwan, A. Mehmood
{"title":"Treatment against Mutation of PIK3CA Gene Involved in Lung Cancer by Structure Base Pharmacophore Modeling, Virtual Screening and Molecular Docking","authors":"Z. Turk, M. Hamza, Anum Munir, Sajid Khan, Muhammad Rizwan, A. Mehmood","doi":"10.13189/APP.2019.070403","DOIUrl":"https://doi.org/10.13189/APP.2019.070403","url":null,"abstract":"Lung Cancer is a type of serious cancer that causes 1.37 million deaths every year all over the world and accounts for almost 18% of all cancer death. PI3Ks establish a lipid kinase family that is important to integrate disparate dimensions of cell functions including cell survival, vesicular trafficking, proliferation, and cell migration. This is an essential pathway in the oncogenesis and advancement of lung cancer. In preclinical studies, PIK3 inhibitors deliver exploratory antitumor activity. The study was established to realize perception and molecular mechanisms that are crucial for potent inhibitors of PIK3CA. In this research work, mutated proteins of PIK3CA were selected, models of pharmacophore were designed and hit compounds were obtained against reference feature pharmacophore by virtual screening. These hit compounds were then docked with the mutated proteins of PIK3CA. Three important features were shown by Pharmacophores, Hydrogen bond donor (HBD), Hydrogen bond acceptors (HBA) and aromatic rings (AR). Through virtual screening, 8 hit compounds were obtained before docking Lipinski rule of five was applied and the compounds that achieved all properties were docked with mutated proteins of PIK3CA. 3 compounds fulfilled all properties and demonstrated the stability of ligands. It is suggested that these compounds can be used for curing PIK3CA involved in lung cancer and on the basis of shared feature novel compounds can be designed against a mutation in PIK3CA involved in lung cancer.","PeriodicalId":7378,"journal":{"name":"Advances in Pharmacology and Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74574361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Billah, M. A. Rayhan, S. Yousuf, Kashfia Nawrin, JohirRayhan, Elmabruk M. Khengari
{"title":"A Novel Integrated (OF-HC-EPM) Approach to Study Anxiety Related Depressive Behavior in Mice Model: A Comparison of Neuro Standards","authors":"M. Billah, M. A. Rayhan, S. Yousuf, Kashfia Nawrin, JohirRayhan, Elmabruk M. Khengari","doi":"10.13189/APP.2019.070301","DOIUrl":"https://doi.org/10.13189/APP.2019.070301","url":null,"abstract":"Open Field, Hole Cross and EPM are three widely acceptable experimental methods used to evaluate sedative-anxiolytic potential. The theories behind introducing these fields were to challenge the rodents to a novel environment. However, the behavioral changes caused by these environments often get influenced by rodent's identical neurologic conditions. The major challenges faced by the researchers are variations due to first administration against repeated administration, utilizing same rodent for another experiment but in different time or using different rodent for different experiments. Keeping the drawbacks in consideration, the present study undertook a newly modified (OF-HC-EPM) approach to integrate the experimental fields so as to utilize the same rodents with single oral administration for exposure to different fields which had allowed nullifying the risk of individual and time dependent variance. Anxiolytics, atypical antidepressants, selective serotonin reuptake inhibitors, serotonin-norepinephrine reuptake inhibitors, tricyclic antidepressants as well as the combined anxiolytics and antidepressants were administered to Swiss Albino Mice and their respective behavioral changes were observed. The new approach proved to be an essential tool for evaluating neuropharmacological potentials.","PeriodicalId":7378,"journal":{"name":"Advances in Pharmacology and Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80773299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. M. Hosseininejad, Fatemeh Jahanian, Iraj Goli Khatir, Seyed Hossein Montazer, F. Bozorgi, Nazanin Nosrati, F. H. Kiabi
{"title":"Comparison of Divided Dose versus Bolus Administration Morphine Sulfate for Management of Acute Pain in Traumatic Fracture in Patients Admitted to Emergency Department","authors":"S. M. Hosseininejad, Fatemeh Jahanian, Iraj Goli Khatir, Seyed Hossein Montazer, F. Bozorgi, Nazanin Nosrati, F. H. Kiabi","doi":"10.13189/APP.2019.070302","DOIUrl":"https://doi.org/10.13189/APP.2019.070302","url":null,"abstract":"Introduction: Pain is one of the most common reasons that patients visit emergency departments. Morphine has been used extensively in pain management but there is no consensus about the effectiveness of method of administration. The aim of this study was to compare the divided dose vs bolus medication form of morphine sulfate for management of acute pain in traumatic fracture. Methods: we designed a randomized double-blind clinical trial. On basis of inclusion and exclusion criteria, 160 patients with acute traumatic fracture were enrolled to the study and randomly divided into two groups. The first group (bolus group) received 0.1mg/kg morphine and then 0.1mg/kg normal saline every 5 minutes. The second group (titration group) received 0.1mg/kg of morphine in divided doses every 5 minutes. All injections were intravenously. The primary outcome was pain and before of injection, 15 minutes and 60 minutes after injection were recorded. Data were analyzed using SPSS 19. Results: 77 patients included 49 (63.6%) male with mean age of 47.42 ± 13.15 years were in titration group and 83 patients included 47 males (56.6%) with mean age of 52.40 ± 15.62 years were in bolus group. The complication rate was significantly higher in bolus group (75.9% vs 53.2%, P <0.0001). The need to extra morphine injection in bolus and titration group were 62.7% and 28.6%, respectively (P <0.0001). The mean pain score before and at 15 and 60 minutes after injection were 9.56 ± 1.03, 6.61 ± 1.86 and 1.85 ± 1.13 in titration group and 9.76 ± 0.48, 6.53 ± 1.59 and 0.61 ± 0.46 in bolus group, respectively. The pain score was significantly lower in titration group at 60 minutes after injection (P <0.0001). Discussion: The results of this study showed that titration of morphine are a safe and effective method in compared with bolus administration to reducing acute pain in traumatic fracture.","PeriodicalId":7378,"journal":{"name":"Advances in Pharmacology and Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89738743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Diversity of Fungi in the Public Hand-pump Borehole Water in Onueke, Ezza Local Government Area, Ebonyi State, Nigeria","authors":"Onuorah Samuel, N. John, Odibo Frederick","doi":"10.13189/APP.2019.070201","DOIUrl":"https://doi.org/10.13189/APP.2019.070201","url":null,"abstract":"The diversity of fungi in fifteen public hand-pump borehole water in Onueke, Ezza Local Government Area of Ebonyi State, Nigeria was studied during the dry and wet seasons using standard analytical procedures. The total moulds and yeasts counts were 2-8cfu/ml and 1-5cfu/ml respectively during the dry season and 3-12cfu/ml and 1-4cfu/ml respectively during the wet season. More moulds were isolated from the samples during the wet than the dry season while more yeasts were isolated during the dry than the wet season. The fungi were characterized and identified on the basis of their colonial, microscopic, biochemical and molecular characteristics as Candida albicans, Microsporum canis, Aspergillus fumigatus, Geotrichum candidum, Basidiobolus ranarum, Microsporum audouinii, Fonsecaea pedrosoi and Trichosporon cutaneum. More of the boreholes contained Candida albicans than the other isolates during both seasons. Candida albicans occurred most frequently in the water samples during both seasons while Fonsecaea pedrosoi had the lowest frequency of occurrence in the water samples during both seasons. All the isolates were resistant to Griseofulvin while they exhibited varying degree of resistance and sensitivity to the other antifungal agents used. This study indicated that the borehole water samples examined did not comply with the standard established by regulatory bodies for potable water and therefore must be adequately treated before drinking and use for domestic purposes as these fungi have been reported to cause diseases of humans and animals. Boiling, chlorination, sand filtration and exposure to ultraviolet radiation are recommended.","PeriodicalId":7378,"journal":{"name":"Advances in Pharmacology and Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77608534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"In-vivo Assessment of Neuropharmacological Activity of Methanol Bark Extract of Mimosa Pudica in Mice","authors":"M. L. Hossain, S. Sultana","doi":"10.13189/APP.2019.070202","DOIUrl":"https://doi.org/10.13189/APP.2019.070202","url":null,"abstract":"Background: Mimosa pudica, a common plant of Mimosoideae family has been used as Ayurvedic herbal medicine by Bangladeshi and Chinese to treat several diseases such as hemorrhoids, hair loss, arthritis, dysentery, leprosy, jaundice, leukoderma, asthma, uterine problems. The root and bark of the plant is said to display antimicrobial activity. Hence, gargling with a decoction of the root and bark of Mimosa pudica diluted in water may help to treat toothaches. Aim of the study: The present study was designed to evaluate the presence of phytochemical groups and to investigate the neuropharmacological activity of methanol extract of Mimosa pudica (MEMP). Methodology: The neuropharmacological activity was determined by hole cross and open field test using Swiss Albino mice as experimental animal. Results: Phytochemical analysis of Mimosa pudica extract indicated the presence of tannins, alkaloids, terpenoids and flavonoids. The sample showed dose-dependent sedative activity at the doses of 200 and 400 mg/kg body weight compared to standard drug Diazepam (1 mg/kg body weight). Conclusion: MEMP showed significant sedative activity demonstrating that the extract may be useful in the development of a new sedative and anti-anxiety drug.","PeriodicalId":7378,"journal":{"name":"Advances in Pharmacology and Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80689481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Chidambaram, Kumar Venketesan, S. Dharmalingam, R. Siddalingam, T. Ellappan, S. Mandal
{"title":"In-vitro Inhibitory Effects of Polyphenolic Extract of Ichnocarpus Frutescens on Carbohydrate Digestive Enzymes","authors":"K. Chidambaram, Kumar Venketesan, S. Dharmalingam, R. Siddalingam, T. Ellappan, S. Mandal","doi":"10.13189/APP.2019.070103","DOIUrl":"https://doi.org/10.13189/APP.2019.070103","url":null,"abstract":"Background: Ichnocarpus frutescens has been used as Ayurvedic herbal medicine by Gond tribes in India to treat several diseases such as diabetes mellitus and Jaundice. The growing incidence of diabetes mellitus and its complications have headed to the search of novel therapeutic approaches engrossed on preventing postprandial hyperglycemia. The practice of carbohydrate breakdown enzyme inhibitors from plant resources might be a potential approach to prevent glucose absorption with the least adverse effects. Aims: The objective of the present investigation was to provide in-vitro evidence for the potential inhibitory activity of polyphenolic extract (PPE) of Ichnocarpus frutescens on amylase and glucosidase enzymes. Methods: the in the vitro inhibitory effect of polyphenolic extract (PPE) of Ichnocarpus frutescens focused on the evaluation of pancreatic amylase inhibition, rat serum amylase inhibition and rat intestinal glucosidase inhibition of polyphenolic extract (PPE) of Ichnocarpus frutescens by in vitro. The different concentrations of polyphenolic extract were subjected to amylase and glucosidase inhibitory assay and the percentage of amylase and glucosidase inhibitory activity and IC50 values were calculated. Results: Polyphenolic extract shows appreciable pancreatic amylase inhibitory activity in vitro. The extract also showed an appreciable glucosidase inhibitory effect in a concentration-dependent manner with a moderate amylase inhibitory activity. The in vitro examination of the inhibitory effect of PPE on maltase and sucrase activities revealed that PPE inhibited rat small intestine disaccharidase ( glucosidase) activity. Conclusion: Taken together, these results suggest that inhibitory effect of PPE on amylase and glucosidase activities may delay carbohydrate digestion and absorption with subsequent lowering of blood glucose level leading to prevention of postprandial hyperglycemia in diabetes and its complication.","PeriodicalId":7378,"journal":{"name":"Advances in Pharmacology and Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83865508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}