International journal of proteomics最新文献_第2页

Comparative proteomic study reveals the molecular aspects of delayed ocular symptoms induced by sulfur mustard. 比较蛋白质组学研究揭示了硫芥致眼部迟发性症状的分子机制。

International journal of proteomics Pub Date : 2015-01-01 Epub Date: 2015-01-21 DOI: 10.1155/2015/659241

Zaiddodine Pashandi, Neda Saraygord-Afshari, Hossein Naderi-Manesh, Mostafa Naderi

{"title":"Comparative proteomic study reveals the molecular aspects of delayed ocular symptoms induced by sulfur mustard.","authors":"Zaiddodine Pashandi, Neda Saraygord-Afshari, Hossein Naderi-Manesh, Mostafa Naderi","doi":"10.1155/2015/659241","DOIUrl":"https://doi.org/10.1155/2015/659241","url":null,"abstract":"Objective. Sulfur mustard (SM) is a highly reactive alkylating agent which produces ocular, respiratory, and skin damages. Eyes are the most sensitive organ to SM due to high intrinsic metabolic and rapid turnover rate of corneal epithelium and aqueous-mucous interfaces of the cornea and conjunctiva. Here we investigate underlying molecular mechanism of SM exposure delayed effects which is still a controversial issue after about 30 years. Materials and Methods. Following ethical approval, we have analyzed serum proteome of ten severe SM exposed male patients with delayed eye symptoms with two-dimensional electrophoresis followed by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry. The western blotting was used to confirm the proteins that have been identified. Results. We have identified thirteen proteins including albumin, haptoglobin, and keratin isoforms as well as immunoglobulin kappa chain which showed upregulation while transferrin and alpha 1 antitrypsin revealed downregulation in these patients in comparison with healthy control group. Conclusions. Our results elevated participation of free iron circulatory imbalance and local matrix-metalloproteinase activity in development of delayed ocular symptoms induced by SM. It demonstrates that SM induced systemic toxicity leads to some serum protein changes that continually and gradually exacerbate the ocular surface injuries. ","PeriodicalId":73474,"journal":{"name":"International journal of proteomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/659241","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33058137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Quantitative Proteomics and Lipidomics Analysis of Endoplasmic Reticulum of Macrophage Infected with Mycobacterium tuberculosis. 结核分枝杆菌感染巨噬细胞内质网定量蛋白质组学和脂质组学分析。

International journal of proteomics Pub Date : 2015-01-01 Epub Date: 2015-02-16 DOI: 10.1155/2015/270438

Najmuddin Mohd Saquib, Shilpa Jamwal, Mukul Kumar Midha, Hirdya Narain Verma, Venkatasamy Manivel

{"title":"Quantitative Proteomics and Lipidomics Analysis of Endoplasmic Reticulum of Macrophage Infected with Mycobacterium tuberculosis.","authors":"Najmuddin Mohd Saquib, Shilpa Jamwal, Mukul Kumar Midha, Hirdya Narain Verma, Venkatasamy Manivel","doi":"10.1155/2015/270438","DOIUrl":"https://doi.org/10.1155/2015/270438","url":null,"abstract":"Even though endoplasmic reticulum (ER) stress associated with mycobacterial infection has been well studied, the molecular basis of ER as a crucial organelle to determine the fate of Mtb is yet to be established. Here, we have studied the ability of Mtb to manipulate the ultrastructural architecture of macrophage ER and found that the ER-phenotypes associated with virulent (H37Rv) and avirulent (H37Ra) strains were different: a rough ER (RER) with the former against a smooth ER (SER) with the later. Further, the functional attributes of these changes were probed by MS-based quantitative proteomics (133 ER proteins) and lipidomics (8 phospholipids). Our omics approaches not only revealed the host pathogen cross-talk but also emphasized how precisely Mtb uses proteins and lipids in combination to give rise to characteristic ER-phenotypes. H37Ra-infected macrophages increased the cytosolic Ca(2+) levels by attenuating the ATP2A2 protein and simultaneous induction of PC/PE expression to facilitate apoptosis. However, H37Rv inhibited apoptosis and further controlled the expression of EST-1 and AMRP proteins to disturb cholesterol homeostasis resulting in sustained infection. This approach offers the potential to decipher the specific roles of ER in understanding the cell biology of mycobacterial infection with special reference to the impact of host response. ","PeriodicalId":73474,"journal":{"name":"International journal of proteomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/270438","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33142105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

SILAC-Based Quantitative Proteomic Analysis of Diffuse Large B-Cell Lymphoma Patients. 基于silac的弥漫性大b细胞淋巴瘤患者定量蛋白质组学分析。

International journal of proteomics Pub Date : 2015-01-01 Epub Date: 2015-04-28 DOI: 10.1155/2015/841769

Ulla Rüetschi, Martin Stenson, Sverker Hasselblom, Herman Nilsson-Ehle, Ulrika Hansson, Henrik Fagman, Per-Ola Andersson

{"title":"SILAC-Based Quantitative Proteomic Analysis of Diffuse Large B-Cell Lymphoma Patients.","authors":"Ulla Rüetschi, Martin Stenson, Sverker Hasselblom, Herman Nilsson-Ehle, Ulrika Hansson, Henrik Fagman, Per-Ola Andersson","doi":"10.1155/2015/841769","DOIUrl":"https://doi.org/10.1155/2015/841769","url":null,"abstract":"Diffuse large B-cell lymphoma (DLBCL), the most common lymphoma, is a heterogeneous disease where the outcome for patients with early relapse or refractory disease is very poor, even in the era of immunochemotherapy. In order to describe possible differences in global protein expression and network patterns, we performed a SILAC-based shotgun (LC-MS/MS) quantitative proteomic analysis in fresh-frozen tumor tissue from two groups of DLBCL patients with totally different clinical outcome: (i) early relapsed or refractory and (ii) long-term progression-free patients. We could identify over 3,500 proteins; more than 1,300 were quantified in all patients and 87 were significantly differentially expressed. By functional annotation analysis on the 66 proteins overexpressed in the progression-free patient group, we found an enrichment of proteins involved in the regulation and organization of the actin cytoskeleton. Also, five proteins from actin cytoskeleton regulation, applied in a supervised regression analysis, could discriminate the two patient groups. In conclusion, SILAC-based shotgun quantitative proteomic analysis appears to be a powerful tool to explore the proteome in DLBCL tumor tissue. Also, as progression-free patients had a higher expression of proteins involved in the actin cytoskeleton protein network, such a pattern indicates a functional role in the sustained response to immunochemotherapy. ","PeriodicalId":73474,"journal":{"name":"International journal of proteomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/841769","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33376996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

In-depth profiling of the peripheral blood mononuclear cells proteome for clinical blood proteomics. 深入分析外周血单个核细胞蛋白质组用于临床血液蛋白质组学。

International journal of proteomics Pub Date : 2014-01-01 Epub Date: 2014-03-03 DOI: 10.1155/2014/129259

Saša Končarević, Christopher Lößner, Karsten Kuhn, Thorsten Prinz, Ian Pike, Hans-Dieter Zucht

{"title":"In-depth profiling of the peripheral blood mononuclear cells proteome for clinical blood proteomics.","authors":"Saša Končarević, Christopher Lößner, Karsten Kuhn, Thorsten Prinz, Ian Pike, Hans-Dieter Zucht","doi":"10.1155/2014/129259","DOIUrl":"https://doi.org/10.1155/2014/129259","url":null,"abstract":"Peripheral blood mononuclear cells (PBMCs) are an easy accessible cellular part of the blood organ and, along with platelets, represent the only site of active gene expression in blood. These cells undergo immunophenotypic changes in various diseases and represent a peripheral source of monitoring gene expression and posttranslational modifications relevant to many diseases. Little is known about the source of many blood proteins and we hypothesise that release from PBMCs through active and passive mechanisms may account for a substantial part of the plasma proteome. The use of state-of-the-art proteomic profiling methods in PBMCs will enable minimally invasive monitoring of disease progression or response to treatment and discovery of biomarkers. To achieve this goal, detailed mapping of the PBMC proteome using a sensitive, robust, and quantitative methodological setup is required. We have applied an indepth gel-free proteomics approach using tandem mass tags (TMT), unfractionated and SCX fractionated PBMC samples, and LC-MS/MS with various modulations. This study represents a benchmark in deciphering the PBMC proteome as we provide a deep insight by identifying 4129 proteins and 25503 peptides. The identified proteome defines the scope that enables PBMCs to be characterised as cellular major biomarker pool within the blood organ. ","PeriodicalId":73474,"journal":{"name":"International journal of proteomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/129259","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32255730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 43

The influence of flanking secondary structures on amino Acid content and typical lengths of 3/10 helices. 侧翼二级结构对氨基酸含量和典型3/10螺旋长度的影响。

International journal of proteomics Pub Date : 2014-01-01 Epub Date: 2014-10-13 DOI: 10.1155/2014/360230

Vladislav Victorovich Khrustalev, Eugene Victorovich Barkovsky, Tatyana Aleksandrovna Khrustaleva

{"title":"The influence of flanking secondary structures on amino Acid content and typical lengths of 3/10 helices.","authors":"Vladislav Victorovich Khrustalev, Eugene Victorovich Barkovsky, Tatyana Aleksandrovna Khrustaleva","doi":"10.1155/2014/360230","DOIUrl":"https://doi.org/10.1155/2014/360230","url":null,"abstract":"We used 3D structures of a highly redundant set of bacterial proteins encoded by genes of high, average, and low GC-content. Four types of connecting bridges-regions situated between any of two major elements of secondary structure (alpha helices and beta strands)-containing a pure random coil were compared with connecting bridges containing 3/10 helices. We included discovered trends in the original \"VVTAK Connecting Bridges\" algorithm, which is able to predict more probable conformation for a given connecting bridge. The highest number of significant differences in amino acid usage was found between 3/10 helices containing bridges connecting two beta strands (they have increased Phe, Tyr, Met, Ile, Leu, Val, and His usages but decreased usages of Asp, Asn, Gly, and Pro) and those without 3/10 helices. The typical (most common) length of 3/10 helices situated between two beta strands and between beta strand and alpha helix is equal to 5 amino acid residues. The preferred length of 3/10 helices situated between alpha helix and beta strand is equal to 3 residues. For 3/10 helices situated between two alpha helices, both lengths (3 and 5 amino acid residues) are typical. ","PeriodicalId":73474,"journal":{"name":"International journal of proteomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/360230","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32793678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Enhanced Photosynthesis and Carbon Metabolism Favor Arsenic Tolerance in Artemisia annua, a Medicinal Plant as Revealed by Homology-Based Proteomics. 基于同源蛋白组学的研究表明，药用植物黄花蒿光合作用和碳代谢增强有利于其抗砷性。

International journal of proteomics Pub Date : 2014-01-01 Epub Date: 2014-04-29 DOI: 10.1155/2014/163962

Rashmi Rai, Sarita Pandey, Alok Kumar Shrivastava, Shashi Pandey Rai

{"title":"Enhanced Photosynthesis and Carbon Metabolism Favor Arsenic Tolerance in Artemisia annua, a Medicinal Plant as Revealed by Homology-Based Proteomics.","authors":"Rashmi Rai, Sarita Pandey, Alok Kumar Shrivastava, Shashi Pandey Rai","doi":"10.1155/2014/163962","DOIUrl":"https://doi.org/10.1155/2014/163962","url":null,"abstract":"This paper provides the first proteomic evidence of arsenic (As) tolerance and interactive regulatory network between primary and secondary metabolism in the medicinal plant, Artemisia annua. While chlorophyll fluorescence and photosynthetic rate depicted mild inhibition, there was a significant enhancement in PSI activity, whole chain, ATP, and NADPH contents in 100 μ M As treatments compared to the control plants. However, a decrease in the above variables was recorded under 150 μ M treatments. Proteomic decoding of the survival strategy of A. annua under As stress using 2-DE followed by MALDI-MS/MS revealed a total of 46 differentially expressed protein spots. In contrast to other plants where As inhibits photosynthesis, A. annua showed appreciable photosynthetic CO2 assimilation and allocation of carbon resources at 100 μ M As concentration. While an increased accumulation of ATP synthase, ferredoxin-NADP(H) oxidoreductase, and FeS-rieske proteins supported the operation of cyclic electron transport, mdr ABC transporter protein and pcs gene might be involved in As detoxification. The most interesting observation was an increased accumulation of LEAFY like novel protein conceivably responsible for an early onset of flowering in A. annua under As stress. This study not only affirmed the role of energy metabolism proteins but also identified potential candidates responsible for As tolerance in plants. ","PeriodicalId":73474,"journal":{"name":"International journal of proteomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/163962","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32372929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Mapping and Identification of the Urine Proteome of Prostate Cancer Patients by 2D PAGE/MS. 前列腺癌患者尿液蛋白质组的2D PAGE/MS定位与鉴定

International journal of proteomics Pub Date : 2014-01-01 Epub Date: 2014-08-20 DOI: 10.1155/2014/594761

Sanja Kiprijanovska, Sotir Stavridis, Oliver Stankov, Selim Komina, Gordana Petrusevska, Momir Polenakovic, Katarina Davalieva

{"title":"Mapping and Identification of the Urine Proteome of Prostate Cancer Patients by 2D PAGE/MS.","authors":"Sanja Kiprijanovska, Sotir Stavridis, Oliver Stankov, Selim Komina, Gordana Petrusevska, Momir Polenakovic, Katarina Davalieva","doi":"10.1155/2014/594761","DOIUrl":"https://doi.org/10.1155/2014/594761","url":null,"abstract":"Proteome analysis of the urine has shown that urine contains disease-specific information for a variety of urogenital system disorders, including prostate cancer (PCa). The aim of this study was to determine the protein components of urine from PCa patients. Urine from 8 patients with clinically and histologically confirmed PCa was analyzed by conventional 2D PAGE. The MS identification of the most prominent 125 spots from the urine map revealed 45 distinct proteins. According to Gene Ontology, the identified proteins are involved in a variety of biological processes, majority of them are secreted (71%), and half of them are enzymes or transporters. Comparison with the normal urine proteome revealed 11 proteins distinctive for PCa. Using Ingenuity Pathways Analysis, we have found 3 proteins (E3 ubiquitin-protein ligase rififylin, tumor protein D52, and thymidine phosphorylase) associated with cellular growth and proliferation (p = 8.35 × 10(-4) - 3.41 × 10(-2)). The top network of functional associations between 11 proteins was Cell Death and Survival, Cell-To-Cell Signaling and Interaction, and System Development and Function (p = 10(-30)). In summary, we have created an initial proteomic map of PCa patient's urine. The results from this study provide some leads to understand the molecular bases of prostate cancer. ","PeriodicalId":73474,"journal":{"name":"International journal of proteomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/594761","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32661944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

A method to determine lysine acetylation stoichiometries. 一种测定赖氨酸乙酰化化学计量的方法。

International journal of proteomics Pub Date : 2014-01-01 Epub Date: 2014-07-20 DOI: 10.1155/2014/730725

Ernesto S Nakayasu, Si Wu, Michael A Sydor, Anil K Shukla, Karl K Weitz, Ronald J Moore, Kim K Hixson, Jong-Seo Kim, Vladislav A Petyuk, Matthew E Monroe, Ljiljiana Pasa-Tolic, Wei-Jun Qian, Richard D Smith, Joshua N Adkins, Charles Ansong

{"title":"A method to determine lysine acetylation stoichiometries.","authors":"Ernesto S Nakayasu, Si Wu, Michael A Sydor, Anil K Shukla, Karl K Weitz, Ronald J Moore, Kim K Hixson, Jong-Seo Kim, Vladislav A Petyuk, Matthew E Monroe, Ljiljiana Pasa-Tolic, Wei-Jun Qian, Richard D Smith, Joshua N Adkins, Charles Ansong","doi":"10.1155/2014/730725","DOIUrl":"https://doi.org/10.1155/2014/730725","url":null,"abstract":"Lysine acetylation is a common protein posttranslational modification that regulates a variety of biological processes. A major bottleneck to fully understanding the functional aspects of lysine acetylation is the difficulty in measuring the proportion of lysine residues that are acetylated. Here we describe a mass spectrometry method using a combination of isotope labeling and detection of a diagnostic fragment ion to determine the stoichiometry of protein lysine acetylation. Using this technique, we determined the modification occupancy for ~750 acetylated peptides from mammalian cell lysates. Furthermore, the acetylation on N-terminal tail of histone H4 was cross-validated by treating cells with sodium butyrate, a potent deacetylase inhibitor, and comparing changes in stoichiometry levels measured by our method with immunoblotting measurements. Of note we observe that acetylation stoichiometry is high in nuclear proteins, but very low in mitochondrial and cytosolic proteins. In summary, our method opens new opportunities to study in detail the relationship of lysine acetylation levels of proteins with their biological functions. ","PeriodicalId":73474,"journal":{"name":"International journal of proteomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/730725","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32602860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

Protein-protein interaction detection: methods and analysis. 蛋白质-蛋白质相互作用检测:方法与分析。

International journal of proteomics Pub Date : 2014-01-01 Epub Date: 2014-02-17 DOI: 10.1155/2014/147648

V Srinivasa Rao, K Srinivas, G N Sujini, G N Sunand Kumar

{"title":"Protein-protein interaction detection: methods and analysis.","authors":"V Srinivasa Rao, K Srinivas, G N Sujini, G N Sunand Kumar","doi":"10.1155/2014/147648","DOIUrl":"https://doi.org/10.1155/2014/147648","url":null,"abstract":"Protein-protein interaction plays key role in predicting the protein function of target protein and drug ability of molecules. The majority of genes and proteins realize resulting phenotype functions as a set of interactions. The in vitro and in vivo methods like affinity purification, Y2H (yeast 2 hybrid), TAP (tandem affinity purification), and so forth have their own limitations like cost, time, and so forth, and the resultant data sets are noisy and have more false positives to annotate the function of drug molecules. Thus, in silico methods which include sequence-based approaches, structure-based approaches, chromosome proximity, gene fusion, in silico 2 hybrid, phylogenetic tree, phylogenetic profile, and gene expression-based approaches were developed. Elucidation of protein interaction networks also contributes greatly to the analysis of signal transduction pathways. Recent developments have also led to the construction of networks having all the protein-protein interactions using computational methods for signaling pathways and protein complex identification in specific diseases. ","PeriodicalId":73474,"journal":{"name":"International journal of proteomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/147648","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32230104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 515

Comparative Analysis of Sorghum bicolor Proteome in Response to Drought Stress and following Recovery. 高粱双色蛋白质组对干旱胁迫的响应与恢复的比较分析。

International journal of proteomics Pub Date : 2014-01-01 Epub Date: 2014-10-01 DOI: 10.1155/2014/395905

Christoph Jedmowski, Ahmed Ashoub, Tobias Beckhaus, Thomas Berberich, Michael Karas, Wolfgang Brüggemann

引用次数: 58