Hematology and cell therapy最新文献_第5页

A randomized phase II trial of high-dose chlorambucil vs. fludarabine in patients with advanced B-chronic lymphocytic leukemia. EORTC Leukemia Cooperative Group. 晚期b -慢性淋巴细胞白血病患者中大剂量氯苯与氟达拉滨的随机II期试验EORTC白血病合作小组。

Hematology and cell therapy Pub Date : 1999-06-01

引用次数: 0

Gamma-irradiation-induced DNA single- and double-strand breaks and their repair in chronic lymphocytic leukemia cells of variable radiosensitivity. γ辐照诱导的可变放射敏感性慢性淋巴细胞白血病细胞DNA单双链断裂及其修复。

Hematology and cell therapy Pub Date : 1999-06-01 DOI: 10.1007/s00282-999-0095-6

M H Myllyperkiö, T R Koski, L M Vilpo, J A Vilpo

{"title":"Gamma-irradiation-induced DNA single- and double-strand breaks and their repair in chronic lymphocytic leukemia cells of variable radiosensitivity.","authors":"M H Myllyperkiö, T R Koski, L M Vilpo, J A Vilpo","doi":"10.1007/s00282-999-0095-6","DOIUrl":"https://doi.org/10.1007/s00282-999-0095-6","url":null,"abstract":"Gamma-irradiation-induced DNA single- and double-strand break (SSB and DSB) formation and their repair kinetics in normal hematopoietic cells and in leukemic lymphocytes was investigated using alkaline and neutral comet assays. The cells were isolated by density gradient centrifugation from peripheral blood of patients with chronic lymphocytic leukemia (CLL) and from healthy study subjects. Furthermore, CD34+ progenitor cells isolated with immunomagnetic beads from bone marrow of non-leukemic persons were investigated. The cytotoxicity of 137Cs irradiation was determined in vitro in peripheral blood mononuclear lymphocytes from 36 CLL patients and from 8 healthy donors using radioactive leucine incorporation assay in 4-day culture. A dose-dependent increase in DNA migration was observed in alkaline (SSBs) and neutral (DSBs) gel electrophoresis when the cells were exposed to gamma-irradiation doses up to 10.4 Gy. After irradiation with doses of 2.4 and 5.4 Gy, the cells repaired their single- and double-strand breaks almost completely. The formation and repair of DNA strand breaks were essentially similar in all normal cell populations investigated and in CLL cells. The gamma-irradiation-induced cytotoxicity did not correlate with DNA strand break formation and repair capacity. According to these results, the differences of gamma-irradiation tolerance among individual CLL cases and among healthy persons are explicable in terms other than DNA strand break formation or repair.","PeriodicalId":73231,"journal":{"name":"Hematology and cell therapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00282-999-0095-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21320999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

The role of autologous hematopoietic progenitor and cell reinfusion for intensive chemotherapy in women with poor-prognosis breast cancer. Clinical studies with ex-vivo expanded cells produced with the Aastrom Replicell technology. 自体造血祖细胞和细胞再输注在预后不良乳腺癌妇女强化化疗中的作用。用Aastrom Replicell技术生产的离体扩增细胞进行临床研究。

Hematology and cell therapy Pub Date : 1999-04-01 DOI: 10.1007/s00282-999-0078-7

C Chabannon, G Novakovitch, J L Blache, S Olivero, J Camerlo, D Genre, D Maraninchi, P Viens

引用次数: 2

[Intensive chemotherapy and autograft of hematopoietic stem cells in the treatment of metastatic cancer: results of the national protocol Pegase 04]. 【强化化疗和自体造血干细胞移植治疗转移性癌症:国家方案Pegase 04的结果】。

Hematology and cell therapy Pub Date : 1999-04-01 DOI: 10.1007/s00282-999-0071-1

J P Lotz, H Curé, M Janvier, F Morvan, M Legros, B Asselain, M Guillemot, H Roché, C Gisselbrecht

{"title":"[Intensive chemotherapy and autograft of hematopoietic stem cells in the treatment of metastatic cancer: results of the national protocol Pegase 04].","authors":"J P Lotz, H Curé, M Janvier, F Morvan, M Legros, B Asselain, M Guillemot, H Roché, C Gisselbrecht","doi":"10.1007/s00282-999-0071-1","DOIUrl":"https://doi.org/10.1007/s00282-999-0071-1","url":null,"abstract":"Unlabelled: We report hereby the results of the french multicentric randomized PEGASE 04 protocol established to evaluate the impact on survival of high-dose chemotherapy over conventional chemotherapy for MBC patients.Patients and methods: Inclusion criteria were: age < or = 60 year, PS < 2, adenocarcinoma initially metastatic or in first relapse, chemosensitive disease. Randomization was done after 4-6 courses of conventionnal chemotherapy between high-dose (Mitoxantrone, 45 mg/m2, Cyclophosphamide: 120 mg/kg, Melphalan: 140 mg/m2), and the pursuit of the same conventionnal chemotherapy. Between 09/92 and 12/96, 61 chemosensitive patients were enrolled: 29 were referred to standard chemotherapy, 32 to intensive therapy. At randomization, 13 pts (21.3%) were in complete response and 48 in partial response.Results: The median progression-free survivals were 20 and 35.3 months in the standard and intensive groups (p=0.06). The relapse rates were respectively 79.3% vs 50.8% at 3 years and 90.8% vs 90.7% at 5 years. The median overall survivals were 20 and 43.4 months, with an overall survival rate of 18.5% vs 29.8% at 5 years (p=0.12).Conclusion: The CMA regimen could prolong the progression-free survival of MBC patients, however without any significant impact on overall survival.","PeriodicalId":73231,"journal":{"name":"Hematology and cell therapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00282-999-0071-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21214349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Proceedings of the 10th Cell Therapy Workshop. Paris, France, 31 March-1 April 1999. 第十届细胞治疗研讨会论文集。1999年3月31日至4月1日，法国巴黎。

Hematology and cell therapy Pub Date : 1999-04-01

引用次数: 0

Low dose T-cell lymphocyte infusion combined with marrow T-cell depletion as prophylaxis of acute graft vs host disease for HLA identical sibling bone marrow transplantation. 低剂量t淋巴细胞输注联合骨髓t细胞清除预防HLA同卵同胞骨髓移植急性移植物抗宿主病

Hematology and cell therapy Pub Date : 1999-04-01 DOI: 10.1007/s00282-999-0031-9

J Y Cahn, E Deconinck, P Tiberghien, A Brion, E Racadot, M Deschaseaux, R Angonin, L Voillat, J Vuillier, J Fontan, P Morel, J M Cordonnier, S Comparot, M C Woronoff-Lemsi, P Hervé

{"title":"Low dose T-cell lymphocyte infusion combined with marrow T-cell depletion as prophylaxis of acute graft vs host disease for HLA identical sibling bone marrow transplantation.","authors":"J Y Cahn, E Deconinck, P Tiberghien, A Brion, E Racadot, M Deschaseaux, R Angonin, L Voillat, J Vuillier, J Fontan, P Morel, J M Cordonnier, S Comparot, M C Woronoff-Lemsi, P Hervé","doi":"10.1007/s00282-999-0031-9","DOIUrl":"https://doi.org/10.1007/s00282-999-0031-9","url":null,"abstract":"T-cell depletion (TCD) of the bone marrow graft remains the most effective method to prevent severe graft versus host disease after allogeneic bone marrow transplantation. Early studies of HLA-identical sibling transplants showed that although T-cell depletion decreased GVHD, T-cell depleted transplants had higher risks of graft failure and leukemia relapse, leukemia free survival (LFS) was not improved compared to non-T-cell depleted transplants. In order to avoid graft failure and increased risk of relapse associated with this approach, we initiated a pilot study of T-cell depletion of the marrow graft combined with reinfusion of a fixed quantity of CD2+ peripheral blood T-cells. Depletion technique consisted in negative purging using CD2 and CD7 monoclonal antibodies (MoAbs) followed by rabbit complement cytolysis. This approach was associated with an intensified conditioning regimen using total body irradiation, high-dose cytosine arabinoside and melphalan (TAM) for all but one patient. Twenty-one patients were included with a mean age of 40 years. Only one acute severe Graft Versus Host Disease (GVHD) was observed and all patients engrafted. At 63 months, probability of survival is 42.86% with a relapse risk of 19.89%, two patients died from B-cell lymphoproliferative disease, seven other died from the procedure partially because of the use of the TAM as pretransplant regimen. This approach is being pursued by a gene therapy trial using herpes-simplex - 1 thymidine kinase gene expressing peripheral donor T-cells.","PeriodicalId":73231,"journal":{"name":"Hematology and cell therapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00282-999-0031-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21214368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Mécanismes de la progression des carcinomes 癌症进展的机制

Hematology and cell therapy Pub Date : 1999-04-01 DOI: 10.1007/S00282-999-0055-1

J. Thiery

引用次数: 0

Evaluation of the monocyte counting by the ABX Vega. Comparison with the manual method and fluoro-flow cytometry. ABX Vega对单核细胞计数的评价。与手工法和荧光流式细胞术的比较。

Hematology and cell therapy Pub Date : 1999-04-01 DOI: 10.1007/s00282-999-0047-1

M Morillon, J Maslin, J J De Pina, F J Louis, G Martet

{"title":"Evaluation of the monocyte counting by the ABX Vega. Comparison with the manual method and fluoro-flow cytometry.","authors":"M Morillon, J Maslin, J J De Pina, F J Louis, G Martet","doi":"10.1007/s00282-999-0047-1","DOIUrl":"https://doi.org/10.1007/s00282-999-0047-1","url":null,"abstract":"This study allowed evaluation and comparison of the precision (20 samples) and agreement (200 samples) of the relative and absolute values of the monocyte count using three methods: microscopic, flow cytometry and the automated ABX Vega hematology analyzer. Flow cytometry is the most precise method, even if the coefficient of variation of the automated analyzer is very similar. The coefficient of correlation between the ABX Vega and the flow cytometer is very satisfactory (r=0.8042). This study of the agreement also made it possible to confirm the difficulty that automated hematology analyzers have in differentiating between some granulocytes and monocytes and their propensity to overstimate the latter when the sample includes immature forms of granulocytes. This fact stresses the importance of the microscopic method, despite its lack of precision. The observed discrepancies did not lead to any difficulties in clinical interpretation; however, this finding should be taken into consideration, particularly in myleoproliferative syndromes and the laboratory monitoring of chemotherapy.","PeriodicalId":73231,"journal":{"name":"Hematology and cell therapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00282-999-0047-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21214370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Remote tumor cells in the case of breast cancers: significance of their presence for prognosis [corrected]. 乳腺癌病例中的远端肿瘤细胞:其存在对预后的意义[修正]。

Hematology and cell therapy Pub Date : 1999-04-01 DOI: 10.1007/s00282-999-0066-y

L Edelman, J C Durand, J P Lotz, P Validire, E Monchâtre, C Bayle, C Arnoulet, D Sainty

引用次数: 0

High dose chemotherapy with haematopoietic rescue in breast cancer. 大剂量化疗联合造血挽救治疗乳腺癌。

Hematology and cell therapy Pub Date : 1999-04-01 DOI: 10.1007/s00282-999-0058-y

W R Bezwoda

{"title":"High dose chemotherapy with haematopoietic rescue in breast cancer.","authors":"W R Bezwoda","doi":"10.1007/s00282-999-0058-y","DOIUrl":"https://doi.org/10.1007/s00282-999-0058-y","url":null,"abstract":"High dose chemotherapy (HDC) for breast cancer has been used for some 15 years. All studies demonstrate a high response rate with approximately 20% prolonged (> 3 years) disease free survival among patients who achieved complete remission (CR) following HDC. Debate about the value of HDC has centered around the issue of patient selection, including selection by chemotherapy response. Results of one randomised, published trial of HDC versus conventional dose treatment, however, demonstrate a better outcome for the HDC group with a higher CR rate and prolongation of survival. These results have been updated and continue to show a stable proportion of long term complete remitters. In the adjuvant setting one recent trial has demonstrated no benefit from HDC 'consolidation' in 'high-risk' patients. Results of a number of other studies are pending and will help to settle this issue. The article reviews issues including patient selection by prior chemotherapy response, other methods of selection, the chemotherapy regimens used and the timing of HDC. While additional and confirmatory studies are required HDC for breast cancer remains an effective treatment modality.","PeriodicalId":73231,"journal":{"name":"Hematology and cell therapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00282-999-0058-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21214347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6