Global translational medicine最新文献

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Green synthesized zinc oxide nanoparticles induce apoptosis by suppressing PI3K/Akt/mTOR signaling pathway in osteosarcoma MG63 cells 绿色合成氧化锌纳米颗粒通过抑制PI3K/Akt/mTOR信号通路诱导MG63骨肉瘤细胞凋亡
Global translational medicine Pub Date : 2022-05-23 DOI: 10.36922/gtm.v1i1.34
S. Subramaniyan, Yoganathan Kamaraj, Veenayohini Kumaresan, Muthulakshmi Kannaiyan, E. David, Babujanarthanam Ranganathan, Vijayanand Selvaraj, Agilan Balupillai
{"title":"Green synthesized zinc oxide nanoparticles induce apoptosis by suppressing PI3K/Akt/mTOR signaling pathway in osteosarcoma MG63 cells","authors":"S. Subramaniyan, Yoganathan Kamaraj, Veenayohini Kumaresan, Muthulakshmi Kannaiyan, E. David, Babujanarthanam Ranganathan, Vijayanand Selvaraj, Agilan Balupillai","doi":"10.36922/gtm.v1i1.34","DOIUrl":"https://doi.org/10.36922/gtm.v1i1.34","url":null,"abstract":"This study aimed to assess the apoptosis-inducing mechanism of zinc oxide nanoparticles (ZnO NPs) stabilized by Solanum xanthocarpum plant extract in human osteosarcoma MG63 cells. In the present study, we synthesized ZnO NPs from S. xanthocarpum extract and evaluated its anticancer mechanism on MG 63 cells. The synthesized ZnO NPs were characterized by ultraviolet spectroscopy, X-ray crystallography, transmission electron microscopy, energy dispersive X-ray, and Fourier-transform infrared spectroscopy analysis. The mean size of the synthesized ZnO NPs was 21.62 ± 7.45 nm and spherical in shape. The cytotoxicity of ZnO NPs on MG63 cells was determined by MTT assay. The Western blot analysis was carried out to examine the expression of apoptotic and autophagy-related proteins in MG63 cells. The findings of the study reveal that ZnO NPs treatment showed concentration-dependent cytotoxicity, increased lipid peroxidation, decreased antioxidant activity, increased reactive oxygen species generation, and increased DNA damage. In addition, ZnO NPs treatment increased the expression of apoptotic members such as p53, Bax, caspase-3, -8, and -9 while downregulating Bcl-2 expression in MG63 cells. Furthermore, ZnO NPs treatment suppressed the P13K/AKT/mTOR signaling pathway and increased the expression of LC3 and beclin-1 in MG63 cells. The present study demonstrated that ZnO NPs induced apoptosis and autophagy in MG63 cells through modifying apoptotic and autophagy-related proteins.","PeriodicalId":73176,"journal":{"name":"Global translational medicine","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85158425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
A mini-review on quantification of atherosclerosis in hypercholesterolemic mice. 高胆固醇血症小鼠动脉粥样硬化定量研究综述。
Global translational medicine Pub Date : 2022-01-01 Epub Date: 2022-06-14 DOI: 10.36922/gtm.v1i1.76
Hui Chen, Deborah A Howatt, Michael K Franklin, Naofumi Amioka, Hisashi Sawada, Alan Daugherty, Hong S Lu
{"title":"A mini-review on quantification of atherosclerosis in hypercholesterolemic mice.","authors":"Hui Chen,&nbsp;Deborah A Howatt,&nbsp;Michael K Franklin,&nbsp;Naofumi Amioka,&nbsp;Hisashi Sawada,&nbsp;Alan Daugherty,&nbsp;Hong S Lu","doi":"10.36922/gtm.v1i1.76","DOIUrl":"https://doi.org/10.36922/gtm.v1i1.76","url":null,"abstract":"<p><p>Atherosclerosis is a leading cause of morbidity and mortality in many countries. Mice are the most frequently used animal model to study the pathogenesis and molecular mechanisms of atherosclerosis. <i>En face</i> analyses of the aorta and cross-sections of the aortic root are the two common modes for quantifying the severity of atherosclerosis in mice. This mini-review introduces these two methods, discusses their pros and cons, and provides suggestions to optimize the quantification of atherosclerosis, thereby enhancing rigor and reproducibility in preclinical research.</p>","PeriodicalId":73176,"journal":{"name":"Global translational medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345319/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40669281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
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