Gene & protein in disease最新文献

筛选
英文 中文
How the West(ern) was won: Solutions for immunoblotting large and small proteins West(ern)如何获胜:免疫印迹大蛋白和小蛋白的解决方案
Gene & protein in disease Pub Date : 2023-06-30 DOI: 10.36922/gpd.0547
Paula Llabata, Pere Llinàs-Arias
{"title":"How the West(ern) was won: Solutions for immunoblotting large and small proteins","authors":"Paula Llabata, Pere Llinàs-Arias","doi":"10.36922/gpd.0547","DOIUrl":"https://doi.org/10.36922/gpd.0547","url":null,"abstract":"Relative protein quantification is a well-established technique in the vast majority of molecular biology laboratories. However, western blot standard protocols may not detect proteins of certain sizes. When the molecular weight of protein of interest is out of the 10–250 kDa range, its migration through the gel or transfer to the membrane is compromised, making its detection difficult. Here, we present a set of modifications of the standard working procedure for western blotting based on the experience working with small VCP-interacting protein and MAX-gene-associated protein, whose molecular weights are 8 and 350 kDa, respectively. We expect that these adaptations may help researchers to improve their experiments in a cost-effective manner.","PeriodicalId":73136,"journal":{"name":"Gene & protein in disease","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136101451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信