Cellular & molecular biology research最新文献_第5页

Kinetics of calcium transport through purified plasma membrane of buffalo (Bubalus bubalis) spermatozoa: effect of calmodulin-like protein. 钙在水牛精子纯化质膜中的转运动力学:钙调素样蛋白的作用。

Cellular & molecular biology research Pub Date : 1995-01-01

K S Sidhu

引用次数: 0

Formation of the arterial media during vascular development. 血管发育过程中动脉介质的形成

Cellular & molecular biology research Pub Date : 1995-01-01

J M Thayer, K Meyers, C M Giachelli, S M Schwartz

{"title":"Formation of the arterial media during vascular development.","authors":"J M Thayer, K Meyers, C M Giachelli, S M Schwartz","doi":"","DOIUrl":"","url":null,"abstract":"We are still in the earliest stages of studying the molecular biology of vascular development. Key questions, even simple questions, such as the origin of endothelial precursors from the epiblast or the mesoderm, remain largely unanswered. For the smooth muscle cell, we do not even have a satisfactory molecular definition of cell type, because the known cell type-specific markers generally disappear when these cells are placed in vitro, and even in vivo smooth muscle cells identified by location can be very undifferentiated. A few bright spots illuminate this cloudy prospect. We do have endothelial lineage markers, and, given the powerful tools of promoting analysis, it seems likely that we will soon known a lot more about the identification of the endothelial lineage. It is hoped that this will help us understand how patterns of development of the vessel wall are controlled. Similarly, the failure of our early studies to identify molecules responsible for investment by smooth muscle can be seen as an exciting finding. If platelet-derived growth factor, fibroblast growth factor, and transforming growth factor beta are not expressed until after smooth muscle investment, still other as yet unidentified factors must be present to account for this stage of development of the vessel wall.","PeriodicalId":72545,"journal":{"name":"Cellular & molecular biology research","volume":"41 4","pages":"251-62"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19749093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insight into atherosclerosis: a review of c-myc and the vascular smooth muscle cell. 洞察动脉粥样硬化:c-myc和血管平滑肌细胞的综述。

Cellular & molecular biology research Pub Date : 1995-01-01

A M Golja, W Rodino, T F Panetta

引用次数: 0

Remodeling the mammalian heart using transgenesis. 利用转基因技术重塑哺乳动物心脏。

Cellular & molecular biology research Pub Date : 1995-01-01

J Palermo, J Gulick, W Ng, I L Grupp, G Grupp, J Robbins

{"title":"Remodeling the mammalian heart using transgenesis.","authors":"J Palermo, J Gulick, W Ng, I L Grupp, G Grupp, J Robbins","doi":"","DOIUrl":"","url":null,"abstract":"Our objective was to test the hypothesis that, via transgenesis, one can modify the contractile protein complement of the mouse heart. Using a promoter derived from the mouse myosin heavy chain gene (alpha-MyHC), we attempted to remodel the mouse myocardium by ectopically expressing a ventricular form of the myosin light chain 2 (MLC2v) in the atrium. The ability of the heart to maintain contractile isoform stoichiometry was tested by overexpressing the cDNA in both the atria and ventricle. The promoter drove high levels of transgene expression in both cardiac compartments and was controlled in an appropriate manner during development. The data show that ectopic overexpression of a contractile protein isoform can lead to compartment specific replacement. However, if the transgene encodes the isoform that is normally present (e.g., MLC2v expressed in the ventricle), the protein levels remain unaffected, although the transgenic transcript accumulates to very high levels. The basic function and the physiologic or pathophysiologic significance of differential MLC2 isoform content was examined. Using the whole working heart preparation, we show that an MLC2a --> MLC2v shift in the atrium severely affects contractile function and performance.","PeriodicalId":72545,"journal":{"name":"Cellular & molecular biology research","volume":"41 6","pages":"501-9"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19749345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mapping of the beta 2 subunit gene of the GABAA receptor (GABRB2) to human chromosome 5q34 using fluorescence in situ hybridization. 利用荧光原位杂交技术定位GABAA受体(GABRB2) β 2亚基基因到人类染色体5q34

Cellular & molecular biology research Pub Date : 1995-01-01

S J Russek, D H Farb

引用次数: 0

Expression and characterization of mouse cFos protein using the baculovirus expression system: ability to form functional AP-1 complex with coexpressed cJun protein. 用杆状病毒表达系统表达小鼠cFos蛋白及其特性:与共表达cJun蛋白形成功能性AP-1复合物的能力

Cellular & molecular biology research Pub Date : 1995-01-01

C M Corvello, R Metz, R Bravo, M C Armelin

引用次数: 0

The c Fos immunoreactivities in the developing and adult rat cerebella. 发育和成年大鼠小脑c - Fos免疫反应。

Cellular & molecular biology research Pub Date : 1995-01-01

Y Ruan, W W Li, D W Lam, D T Yew

引用次数: 0

Buthionine sulfoximine enhances glutathione-but attenuates glutamate-stimulated cell proliferation. 丁硫氨酸亚砜胺增强谷胱甘肽，但减弱谷氨酸刺激的细胞增殖。

Cellular & molecular biology research Pub Date : 1995-01-01

Y J Kang

{"title":"Buthionine sulfoximine enhances glutathione-but attenuates glutamate-stimulated cell proliferation.","authors":"Y J Kang","doi":"","DOIUrl":"","url":null,"abstract":"Buthionine sulfoximine (BSO) inhibits proliferation of human lung carcinoma A549 cells, and exogenous glutathione (GSH) overcomes the antiproliferative effect. The BSO antiproliferation may result from inhibition of cellular uptake of amino acids, and the antagonistic effect of GSH would result from supplementation of amino acids via the gamma-glutamyl cycle. To explore these possibilities, the present study was undertaken to determine effects of BSO on glutamate- and GSH-stimulated cell proliferation. A549 cells were cultured in a glutamine-deficient Dulbecco's modified Eagle's medium (Gln-(-)DMEM), in which they did not proliferate. Addition of glutamate or GSH in the medium to a concentration of 4 mM stimulated cell proliferation. BSO of 0.1 mM enhanced the GSH-stimulated cell proliferation and attenuated the glutamate-stimulated cell proliferation. This BSO effect correlated with changes in cellular glutamate levels; that is, BSO increased and decreased glutamate concentrations, respectively, in GSH- and glutamate-stimulated cells. GSH or glutamate alone significantly increased cellular GSH levels. BSO depleted cellular GSH in both GSH- and glutamate-stimulated cells to the same level. These changes in GSH levels did not correlate with the respective growth modulatory effect. Because BSO inhibits cellular uptake of some amino acids and the A549 cells contain high levels of gamma-glutamyl transpeptidase activity, the results suggest that the BSO inhibition of glutamate-stimulated cell proliferation may result from decreased glutamate uptake. GSH would supplement the cells with glutamate via the gamma-glutamyl pathway to bypass the inhibition of amino acid uptake and overcome the BSO-antiproliferative effect.","PeriodicalId":72545,"journal":{"name":"Cellular & molecular biology research","volume":"41 2","pages":"131-6"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19561029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural characterization and specificity of expression of E2F-5: a new member of the E2F family of transcription factors. E2F-5: E2F转录因子家族的新成员的结构特征和表达特异性

Cellular & molecular biology research Pub Date : 1995-01-01

A Itoh, S F Levinson, T Morita, S Kourembanas, J S Brody, S A Mitsialis

{"title":"Structural characterization and specificity of expression of E2F-5: a new member of the E2F family of transcription factors.","authors":"A Itoh, S F Levinson, T Morita, S Kourembanas, J S Brody, S A Mitsialis","doi":"","DOIUrl":"","url":null,"abstract":"Members of the E2F gene family are transcription factors that have been implicated in the control of genes essential for cell cycle progression. Regulation of E2F function is finely tuned by the retinoblastoma tumor suppressor gene product and a small family of related \"pocket proteins,\" with the participation of a number of cyclins and cyclin-dependent kinases. Perturbations of this regulatory network can lead to oncogenic transformation and, in certain systems, to the loss of the ability to maintain terminal differentiation. We describe here the cloning, structural characterization, and tissue expression pattern of a new member of the E2F family, E2F-5. We show that this protein is highly conserved between human and rat but exhibits considerable divergence from E2F-1, E2F-2, or E2F3. Together with the recently reported E2F-4, E2F-5 defines a new branch of the E2F family. The distribution of E2F-5 mRNA among adult rat tissues and the temporal pattern of its expression during the cell cycle of vascular smooth muscle cells are distinctly different from that of E2F-1. The structural divergence between the two branches of the E2F family may thus reflect participation in different regulatory networks.","PeriodicalId":72545,"journal":{"name":"Cellular & molecular biology research","volume":"41 3","pages":"147-54"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19570067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of cleavage by restriction endonucleases due to modifications induced in SV40 DNA by methyl methanesulfonate. 甲基磺酸诱导SV40 DNA修饰的限制性内切酶对切割的抑制作用。

Cellular & molecular biology research Pub Date : 1995-01-01

S Ghaskadbi, S Bharathi, S P Modak

引用次数: 0