Biophysics reviews最新文献_第2页

Regulation of epithelial cell jamming transition by cytoskeleton and cell-cell interactions. 细胞骨架和细胞间相互作用对上皮细胞干扰转换的调控。

IF 2.9

Biophysics reviews Pub Date : 2024-10-14 eCollection Date: 2024-12-01 DOI: 10.1063/5.0220088

Zoe D Latham, Alexandra Bermudez, Jimmy K Hu, Neil Y C Lin

{"title":"Regulation of epithelial cell jamming transition by cytoskeleton and cell-cell interactions.","authors":"Zoe D Latham, Alexandra Bermudez, Jimmy K Hu, Neil Y C Lin","doi":"10.1063/5.0220088","DOIUrl":"10.1063/5.0220088","url":null,"abstract":"Multicellular systems, such as epithelial cell collectives, undergo transitions similar to those in inert physical systems like sand piles and foams. To remodel or maintain tissue organization during development or disease, these collectives transition between fluid-like and solid-like states, undergoing jamming or unjamming transitions. While these transitions share principles with physical systems, understanding their regulation and implications in cell biology is challenging. Although cell jamming and unjamming follow physics principles described by the jamming diagram, they are fundamentally biological processes. In this review, we explore how cellular processes and interactions regulate jamming and unjamming transitions. We begin with an overview of how these transitions control tissue remodeling in epithelial model systems and describe recent findings of the physical principles governing tissue solidification and fluidization. We then explore the mechanistic pathways that modulate the jamming phase diagram axes, focusing on the regulation of cell fluctuations and geometric compatibility. Drawing upon seminal works in cell biology, we discuss the roles of cytoskeleton and cell-cell adhesion in controlling cell motility and geometry. This comprehensive view illustrates the molecular control of cell jamming and unjamming, crucial for tissue remodeling in various biological contexts.","PeriodicalId":72405,"journal":{"name":"Biophysics reviews","volume":"5 4","pages":"041301"},"PeriodicalIF":2.9,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11479637/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Capturing the illusive ring-shaped intermediates in Aβ42 amyloid formation. 捕捉 Aβ42 淀粉样蛋白形成过程中虚幻的环形中间体

IF 2.9

Biophysics reviews Pub Date : 2024-08-13 eCollection Date: 2024-09-01 DOI: 10.1063/5.0222349

Yu Yuan, Xiaozhe Dong, Huan Wang, Feng Gai

引用次数: 0

Decoding physical principles of cell migration under controlled environment using microfluidics. 利用微流体技术解码细胞在受控环境下迁移的物理原理。

IF 2.9

Biophysics reviews Pub Date : 2024-07-29 eCollection Date: 2024-09-01 DOI: 10.1063/5.0199161

Young Joon Suh, Alan T Li, Mrinal Pandey, Cassidy S Nordmann, Yu Ling Huang, Mingming Wu

{"title":"Decoding physical principles of cell migration under controlled environment using microfluidics.","authors":"Young Joon Suh, Alan T Li, Mrinal Pandey, Cassidy S Nordmann, Yu Ling Huang, Mingming Wu","doi":"10.1063/5.0199161","DOIUrl":"10.1063/5.0199161","url":null,"abstract":"Living cells can perform incredible tasks that man-made micro/nano-sized robots have not yet been able to accomplish. One example is that white blood cells can sense and move to the site of pathogen attack within minutes. The robustness and precision of cellular functions have been perfected through billions of years of evolution. In this context, we ask the question whether cells follow a set of physical principles to sense, adapt, and migrate. Microfluidics has emerged as an enabling technology for recreating well-defined cellular environment for cell migration studies, and its ability to follow single cell dynamics allows for the results to be amenable for theoretical modeling. In this review, we focus on the development of microfluidic platforms for recreating cellular biophysical (e.g., mechanical stress) and biochemical (e.g., nutrients and cytokines) environments for cell migration studies in 3D. We summarize the basic principles that cells (including bacteria, algal, and mammalian cells) use to respond to chemical gradients learned from microfluidic systems. We also discuss about novel biological insights gained from studies of cell migration under biophysical cues and the need for further quantitative studies of cell function under well-controlled biophysical environments in the future.","PeriodicalId":72405,"journal":{"name":"Biophysics reviews","volume":"5 3","pages":"031302"},"PeriodicalIF":2.9,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11290890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141876856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visualizing the degradation of fibrin fibers. 纤维蛋白纤维降解的可视化。

IF 2.9

Biophysics reviews Pub Date : 2024-07-01 eCollection Date: 2024-09-01 DOI: 10.1063/5.0220356

Rebecca A Risman, Valerie Tutwiler

{"title":"Visualizing the degradation of fibrin fibers.","authors":"Rebecca A Risman, Valerie Tutwiler","doi":"10.1063/5.0220356","DOIUrl":"10.1063/5.0220356","url":null,"abstract":"Polymeric fibrin provides the structural and mechanical stability of a blood clot. Fibrin fibers are rod-like and create a network mesh that holds blood cells. When a clot has performed its physiological function in wound healing and preventing excessive blood loss, it must be resolved by the enzymatic degradation of fibrin, otherwise known as fibrinolysis. If a blood clot forms when or where it is not needed, as occurs in ischemic strokes and myocardial infarctions, the blood clot (thrombus) can obstruct blood flow to downstream organs. Obstructive thrombi must be degraded or removed to prevent further complications. If a clot is not degraded on its own, lytic agents (i.e., tissue plasminogen activator, tPA) are given exogenously to induce fibrinolysis. Here, we fluorescently labeled both fibrin and tPA to visualize degradation at the edge of the clot. The fibers with bound tPA were looped or coiled while the fibers farther into the clot remain straight and stable displaying the diffusion of tPA and depth of lysis. This image provides (1) a new method to monitor fibrinolysis with a commercially available chamber with convenient inlets and (2) the visualization of tPA-bound fibrin and the behavior of fibers during degradation. Future work could utilize this technique to study tPA molecule and fibrin interactions, lysis front degradation, and fibrin fiber linearity to understand the mechanisms of intermolecular dynamics dependent on network structure. An enhanced insight into this process can aid in the development of optimized therapeutics to target stubborn clots.","PeriodicalId":72405,"journal":{"name":"Biophysics reviews","volume":"5 3","pages":"032101"},"PeriodicalIF":2.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11219076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141499799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered heart tissue: Design considerations and the state of the art. 工程心脏组织：设计考虑因素和最新技术。

IF 2.9

Biophysics reviews Pub Date : 2024-06-20 eCollection Date: 2024-06-01 DOI: 10.1063/5.0202724

Ilhan Gokhan, Thomas S Blum, Stuart G Campbell

引用次数: 0

Macrophages on the wrinkle: Exploring microscale interactions with substrate topography. 皱纹上的巨噬细胞：探索微观尺度上与基底形貌的相互作用。

Biophysics reviews Pub Date : 2024-06-11 eCollection Date: 2024-06-01 DOI: 10.1063/5.0215563

Francesca Cecilia Lauta, Luca Pellegrino, Roberto Rusconi

引用次数: 0

How cytoskeletal crosstalk makes cells move: Bridging cell-free and cell studies. 细胞骨架串联如何使细胞运动：无细胞研究与细胞研究的桥梁

Biophysics reviews Pub Date : 2024-06-03 eCollection Date: 2024-06-01 DOI: 10.1063/5.0198119

James P Conboy, Irene Istúriz Petitjean, Anouk van der Net, Gijsje H Koenderink

引用次数: 0

The magnetocardiogram. 磁心动图

IF 2.9

Biophysics reviews Pub Date : 2024-05-29 eCollection Date: 2024-06-01 DOI: 10.1063/5.0201950

Bradley J Roth

引用次数: 0

Looking both ways: Electroactive biomaterials with bidirectional implications for dynamic cell-material crosstalk. 双向观察：电活性生物材料对细胞与材料之间的动态串扰具有双向影响。

IF 2.9

Biophysics reviews Pub Date : 2024-05-08 eCollection Date: 2024-06-01 DOI: 10.1063/5.0181222

Kathryn Kwangja Lee, Natalie Celt, Herdeline Ann M Ardoña

{"title":"Looking both ways: Electroactive biomaterials with bidirectional implications for dynamic cell-material crosstalk.","authors":"Kathryn Kwangja Lee, Natalie Celt, Herdeline Ann M Ardoña","doi":"10.1063/5.0181222","DOIUrl":"10.1063/5.0181222","url":null,"abstract":"Cells exist in natural, dynamic microenvironmental niches that facilitate biological responses to external physicochemical cues such as mechanical and electrical stimuli. For excitable cells, exogenous electrical cues are of interest due to their ability to stimulate or regulate cellular behavior via cascade signaling involving ion channels, gap junctions, and integrin receptors across the membrane. In recent years, conductive biomaterials have been demonstrated to influence or record these electrosensitive biological processes whereby the primary design criterion is to achieve seamless cell-material integration. As such, currently available bioelectronic materials are predominantly engineered toward achieving high-performing devices while maintaining the ability to recapitulate the local excitable cell/tissue microenvironment. However, such reports rarely address the dynamic signal coupling or exchange that occurs at the biotic-abiotic interface, as well as the distinction between the ionic transport involved in natural biological process and the electronic (or mixed ionic/electronic) conduction commonly responsible for bioelectronic systems. In this review, we highlight current literature reports that offer platforms capable of bidirectional signal exchange at the biotic-abiotic interface with excitable cell types, along with the design criteria for such biomaterials. Furthermore, insights on current materials not yet explored for biointerfacing or bioelectronics that have potential for bidirectional applications are also provided. Finally, we offer perspectives aimed at bringing attention to the coupling of the signals delivered by synthetic material to natural biological conduction mechanisms, areas of improvement regarding characterizing biotic-abiotic crosstalk, as well as the dynamic nature of this exchange, to be taken into consideration for material/device design consideration for next-generation bioelectronic systems.","PeriodicalId":72405,"journal":{"name":"Biophysics reviews","volume":"5 2","pages":"021303"},"PeriodicalIF":2.9,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11087870/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140913560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep learning from latent spatiotemporal information of the heart: Identifying advanced bioimaging markers from echocardiograms. 从潜在的心脏时空信息中进行深度学习：从超声心动图中识别高级生物成像标记。

IF 2.9

Biophysics reviews Pub Date : 2024-03-27 eCollection Date: 2024-03-01 DOI: 10.1063/5.0176850

Amanda Chang, Xiaodong Wu, Kan Liu

{"title":"Deep learning from latent spatiotemporal information of the heart: Identifying advanced bioimaging markers from echocardiograms.","authors":"Amanda Chang, Xiaodong Wu, Kan Liu","doi":"10.1063/5.0176850","DOIUrl":"10.1063/5.0176850","url":null,"abstract":"A key strength of echocardiography lies in its integration of comprehensive spatiotemporal cardiac imaging data in real-time, to aid frontline or bedside patient risk stratification and management. Nonetheless, its acquisition, processing, and interpretation are known to all be subject to heterogeneity from its reliance on manual and subjective human tracings, which challenges workflow and protocol standardization and final interpretation accuracy. In the era of advanced computational power, utilization of machine learning algorithms for big data analytics in echocardiography promises reduction in cost, cognitive errors, and intra- and inter-observer variability. Novel spatiotemporal deep learning (DL) models allow the integration of temporal arm information based on unlabeled pixel echocardiographic data for convolution of an adaptive semantic spatiotemporal calibration to construct personalized 4D heart meshes, assess global and regional cardiac function, detect early valve pathology, and differentiate uncommon cardiovascular disorders. Meanwhile, data visualization on spatiotemporal DL prediction models helps extract latent temporal imaging features to develop advanced imaging biomarkers in early disease stages and advance our understanding of pathophysiology to support the development of personalized prevention or treatment strategies. Since portable echocardiograms have been increasingly used as point-of-care imaging tools to aid rural care delivery, the application of these new spatiotemporal DL techniques show the potentials in streamlining echocardiographic acquisition, processing, and data analysis to improve workflow standardization and efficiencies, and provide risk stratification and decision supporting tools in real-time, to prompt the building of new imaging diagnostic networks to enhance rural healthcare engagement.","PeriodicalId":72405,"journal":{"name":"Biophysics reviews","volume":"5 1","pages":"011304"},"PeriodicalIF":2.9,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10978053/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140337882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0