Biophysica最新文献_第9页

Supramolecular-Covalent Peptides Self-Assembly: From Design to Regenerative Medicine and Beyond 超分子共价肽自组装：从设计到再生医学及其他

Biophysica Pub Date : 2022-10-11 DOI: 10.3390/biophysica2040030

R. Pugliese

{"title":"Supramolecular-Covalent Peptides Self-Assembly: From Design to Regenerative Medicine and Beyond","authors":"R. Pugliese","doi":"10.3390/biophysica2040030","DOIUrl":"https://doi.org/10.3390/biophysica2040030","url":null,"abstract":"The field of supramolecular peptides self-assembly has undergone outstanding growth since the early 1990s after the serendipitously discovery by Shuguang Zhang of an ionic self-complementary peptide as a repeating segment in a yeast protein. From then on, the field expanded at an accelerating pace and these self-assembled materials have become an integral part of a broad plethora of designer supramolecular nanomaterials useful for different applications ranging from 3D tissue cell cultures, regenerative medicine, up to optoelectronics. However, the supramolecular peptide based-nanomaterials available thus far for regenerative medicine still lack the dynamic complexity found in the biological structures that mediate regeneration. Indeed, self-assembling peptide (SAPs) suffer from poor mechanical stability, losing mechanical properties at low strains. Just like the extracellular matrix (ECM) of living systems, the chemical structure of the SAP-biomaterials should concurrently contain non-covalent and covalent bonds, bringing, respectively, infinite and finite lifetimes of interactions to obtain a reversibly dynamic matrix. In this review, will be highlighted the major advantages and current limitations of SAP-based biomaterials, and it will be discussed the most widely used strategies for precisely tune their mechanical properties (stiffness, resilience, strain-failure, stress resistance), describing recent and promising approaches in tissue engineering, regenerative medicine, and beyond.","PeriodicalId":72401,"journal":{"name":"Biophysica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44170537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Non-Contacting Plant Health Monitoring via Ultrasound in Ambient Air 环境空气超声非接触式植物健康监测

Biophysica Pub Date : 2022-09-27 DOI: 10.3390/biophysica2040029

Teng Yang, Yuqi Jin, N. Dahotre, A. Neogi

引用次数: 0

Comparative Characteristics of Biomaterials from Juvenile Dentin and Brefomatrix Using Raman Spectroscopy 用拉曼光谱比较幼年牙本质和牙胚基质生物材料的特征

Biophysica Pub Date : 2022-09-26 DOI: 10.3390/biophysica2040028

E. Timchenko, I. Bazhutova, P. Timchenko, O. Frolov, L. Volova

引用次数: 0

Fibonacci Sequences, Symmetry and Order in Biological Patterns, Their Sources, Information Origin and the Landauer Principle 斐波那契数列，生物模式中的对称性和有序性，它们的来源，信息起源和朗道尔原理

Biophysica Pub Date : 2022-09-16 DOI: 10.3390/biophysica2030027

E. Bormashenko

{"title":"Fibonacci Sequences, Symmetry and Order in Biological Patterns, Their Sources, Information Origin and the Landauer Principle","authors":"E. Bormashenko","doi":"10.3390/biophysica2030027","DOIUrl":"https://doi.org/10.3390/biophysica2030027","url":null,"abstract":"Physical roots, exemplifications and consequences of periodic and aperiodic ordering (represented by Fibonacci series) in biological systems are discussed. The physical and biological roots and role of symmetry and asymmetry appearing in biological patterns are addressed. A generalization of the Curie–Neumann principle as applied to biological objects is presented, briefly summarized as: “asymmetry is what creates a biological phenomenon”. The “top-down” and “bottom-up” approaches to the explanation of symmetry in organisms are presented and discussed in detail. The “top-down” approach implies that the symmetry of the biological structure follows the symmetry of the media in which this structure is functioning; the “bottom-up” approach, in turn, accepts that the symmetry of biological structures emerges from the symmetry of molecules constituting the structure. A diversity of mathematical measures applicable for quantification of order in biological patterns is introduced. The continuous, Shannon and Voronoi measures of symmetry/ordering and their application to biological objects are addressed. The fine structure of the notion of “order” is discussed. Informational/algorithmic roots of order inherent in the biological systems are considered. Ordered/symmetrical patterns provide an economy of biological information, necessary for the algorithmic description of a biological entity. The application of the Landauer principle bridging physics and theory of information to the biological systems is discussed.","PeriodicalId":72401,"journal":{"name":"Biophysica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43900920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

The Counteraction of Cultivated Cistus creticus L. (Rock Rose) Plants to the Strain Imposed by a Long-Term Exposure to Non-Ionizing Radiation and the Role of DDC 石玫瑰栽培植物对长期非电离辐射胁迫的拮抗作用及DDC的作用

Biophysica Pub Date : 2022-09-01 DOI: 10.3390/biophysica2030024

Aikaterina L. Stefi, Georgia Kalouda, A. Skouroliakou, D. Vassilacopoulou, N. Christodoulakis

{"title":"The Counteraction of Cultivated Cistus creticus L. (Rock Rose) Plants to the Strain Imposed by a Long-Term Exposure to Non-Ionizing Radiation and the Role of DDC","authors":"Aikaterina L. Stefi, Georgia Kalouda, A. Skouroliakou, D. Vassilacopoulou, N. Christodoulakis","doi":"10.3390/biophysica2030024","DOIUrl":"https://doi.org/10.3390/biophysica2030024","url":null,"abstract":"Two groups of Cistus creticus seedlings were grown in two chambers under controlled environmental conditions. In one of the chambers, a continuously emitting base unit of a wireless telephone was placed. After fifty days of culture, the two groups of plants were removed and thoroughly investigated and compared. The aboveground parts of the exposed plants were retarded in development while their roots exhibited increased biomass, compared to the controls. There was a minor decrease in the absorbance of the photosynthetic pigments in exposed plants, while an overproduction of Reactive Oxygen Species (ROS) ROS in their leaves and roots was detected. The expression of the L-Dopa decarboxylase (DDC) seemed to “erupt” following the exposure to radiation in both shoots and roots of the stressed plants, and their roots slow down their secondary development; strangely, the phenolic content is reduced in their leaves, the external topography of which indicates a rather xeromorphic response. We may suggest that Cistus creticus plants, forced by the radiation stress, can finely tune their metabolic pathways in a way that can be useful in the pharmaceutical industry.","PeriodicalId":72401,"journal":{"name":"Biophysica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46898403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunological Effects of Cold Atmospheric Plasma-Treated Cells in Comparison with Those of Cells Treated with Lactaptin-Based Anticancer Drugs 冷空气等离子体处理的细胞与用基于内酰胺的抗癌药物处理的细胞的免疫效果的比较

Biophysica Pub Date : 2022-09-01 DOI: 10.3390/biophysica2030025

O. Troitskaya, D. Novak, M. Varlamov, M. Biryukov, A. Nushtaeva, G. Kochneva, D. Zakrevsky, I. Schweigert, V. Richter, O. Koval

{"title":"Immunological Effects of Cold Atmospheric Plasma-Treated Cells in Comparison with Those of Cells Treated with Lactaptin-Based Anticancer Drugs","authors":"O. Troitskaya, D. Novak, M. Varlamov, M. Biryukov, A. Nushtaeva, G. Kochneva, D. Zakrevsky, I. Schweigert, V. Richter, O. Koval","doi":"10.3390/biophysica2030025","DOIUrl":"https://doi.org/10.3390/biophysica2030025","url":null,"abstract":"The ability of dying cancer cells to induce an anticancer immune response can increase the effectiveness of anticancer therapies, and such type of death is termed immunogenic cell death (ICD). Cells can die along the ICD pathway when exposed not only to chemo- and immunotherapeutics, but also to various types of radiation, such as ionizing radiation and cold atmospheric plasma jets (CAP). We have previously shown that CAP, lactaptin, and a recombinant vaccinia virus encoding lactaptin induce in vitro molecular changes typical of ICD in cancer cells. In the current work, we treated MX-7 rhabdomyosarcoma cells with CAP and lactaptin-based anticancer drugs and evaluated the immunological effects of the treated cells. We showed that dendritic cells (DCs) captured cells treated with various ICD inducers with different efficiency. CAP-treated cells were weakly potent in inducing the maturation of DCs according to MHC II externalization. Moreover, CAP-treated cells were worse in the stimulation of IFN-α release in vitro and were poorly captured by spleen DCs in vivo. Under the irradiation conditions used, CAP was not capable of activating a significant immunological anti-tumor effect in vivo. It is possible that modifications of the CAP irradiation regimen will enhance the activation of the immune system.","PeriodicalId":72401,"journal":{"name":"Biophysica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44314284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Physicochemical Characterization of the Catalytic Unit of Hammerhead Ribozyme and Its Relationship with the Catalytic Activity 锤头核酶催化单元的理化性质及其与催化活性的关系

Biophysica Pub Date : 2022-08-25 DOI: 10.3390/biophysica2030022

Yoshiyuki Tanaka, Daichi Yamanaka, Saori Morioka, Taishi Yamaguchi, Masayuki Morikawa, T. Kodama, Vladimír Sychrovský, C. Kojima, Yoshikazu Hattori

{"title":"Physicochemical Characterization of the Catalytic Unit of Hammerhead Ribozyme and Its Relationship with the Catalytic Activity","authors":"Yoshiyuki Tanaka, Daichi Yamanaka, Saori Morioka, Taishi Yamaguchi, Masayuki Morikawa, T. Kodama, Vladimír Sychrovský, C. Kojima, Yoshikazu Hattori","doi":"10.3390/biophysica2030022","DOIUrl":"https://doi.org/10.3390/biophysica2030022","url":null,"abstract":"The catalytic mechanism of hammerhead ribozymes (HHRzs) attracted great attention in relation to the chemical origin of life. However, the basicity (pKa) of the catalytic sites of HHRzs has not been studied so far. As a result, the investigation of the currently assumed mechanism from an experimentally derived pKa value has been impossible. In HHRzs, there exists a highly functionalized structural unit (A9-G10.1 site) with a catalytic residue (G12) for the nucleophile activation and metal ion-binding residue (G10.1). As inferred from this fact, there might be a possibility that HHRzs may utilize specific functions of the A9-G10.1 motif for the catalytic reaction. Therefore, here we studied the basicity of G12/G10.1-corresponding residues using RNA duplexes including the A9-G10.1 motif without other conserved residues of HHRzs. From the pH-titration experiments with NMR spectra, we have obtained the intrinsic basicity of the G12/G10.1-corresponding residues in the motif, with pKa > 11.5 (N1 of G12) and pKa 4.5 (N7 of G10.1) for the first time. Based on the derived irregular basicity, their correlation with a catalytic activity and a metal affinity were investigated. In total, the derived thermodynamic properties are an intrinsic nature of the exclusive catalytic unit of HHRzs, which will be an outstanding pivot point for the mechanistic analyses.","PeriodicalId":72401,"journal":{"name":"Biophysica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43364797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the Molecular Driving Force of Protein–Protein Association 蛋白质-蛋白质结合的分子驱动力研究

Biophysica Pub Date : 2022-08-25 DOI: 10.3390/biophysica2030023

R. Rapuano, G. Graziano

引用次数: 1

Peptide-Functionalized Nanoparticles for the Targeted Delivery of Cytotoxins to MMP-14-Expressing Cancer Cells 肽功能化纳米粒子向表达MMP-14的癌症细胞靶向递送细胞毒素

Biophysica Pub Date : 2022-08-24 DOI: 10.3390/biophysica2030021

J. Cathcart, Giulia Suarato, Weiyi Li, Jian Cao, Y. Meng

{"title":"Peptide-Functionalized Nanoparticles for the Targeted Delivery of Cytotoxins to MMP-14-Expressing Cancer Cells","authors":"J. Cathcart, Giulia Suarato, Weiyi Li, Jian Cao, Y. Meng","doi":"10.3390/biophysica2030021","DOIUrl":"https://doi.org/10.3390/biophysica2030021","url":null,"abstract":"As 90% of cancer-patient deaths are due to metastasis, novel therapeutics that selectively target and kill metastatic cells are desperately needed. Matrix metalloproteinase-14 (MMP-14), which plays a critical role in digesting the basement membrane and in inducing cancer cell migration, has been found to be expressed at the cell surface of circulating and metastasized tumor cells in various human cancers. We have recently shown that the IVS4 peptide, which mimics the minimal binding motif of the hemopexin-like (PEX) domain of MMP-14, interrupts MMP-14 dimerization and decreases MMP-14-mediated cell invasion. In this study, cancer-homing nanocarriers were assembled by linking IVS4 to polysaccharide-based nanoparticles (NPs), followed by the encapsulation of a pharmaceutical agent. IVS4-NPs efficiently prevented MMP-14-mediated cell migration and conferred an uptake advantage compared to the control peptide in an MMP-14-dependent manner. While the IVS4-NPs alone were not cytotoxic, drug-encapsulated NPs were shown to effectively target MMP-14-expressing cancer cells. This novel nanotherapeutic is capable of inhibiting MMP-14-mediated functions and efficiently killing MMP-14-expressing cancer cells, without affecting the viability of non-cancer cells.","PeriodicalId":72401,"journal":{"name":"Biophysica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44740530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Dynamics of Shikimate Kinase through Molecular Mechanics Shikimate激酶动力学的分子力学探索

Biophysica Pub Date : 2022-08-23 DOI: 10.3390/biophysica2030020

P. Ojeda-May

引用次数: 1