Advanced Biomedical Research最新文献

{"title":"Mortality Rate and Risk Factors in Pediatric Intensive Care Unit of Imam Hossein Children's Hospital in Isfahan: A Prospective Cross-Sectional Study.","authors":"Fatemeh S Hajidavalu,&nbsp;Atefeh Sadeghizadeh","doi":"10.4103/abr.abr_371_21","DOIUrl":"https://doi.org/10.4103/abr.abr_371_21","url":null,"abstract":"<p><strong>Background: </strong>Various studies have conducted to report the mortality rates and its risk factors in pediatric intensive care unit. This study aimed to determine the mortality prevalence and risk factors in PICU of Imam Hossein Children's Hospital in Isfahan, which is the main referral pediatric hospital in the center of Iran.</p><p><strong>Materials and methods: </strong>This study was performed on 311 patients during a period of 9 months. The questionnaire which included age, gender, length of stay in the PICU and hospital, mortality, history of resuscitation in other wards and readmission, the causes and sources of hospitalization, pediatric risk of mortality (PRISM)-III score, respiratory supports, morbidities like nosocomial infections, acute kidney injury (AKI), multiple organ dysfunction syndrome (MODS) confirmed by pediatric sequential organ failure assessment score (P-SOFA) and glycemic disorders was filled out.</p><p><strong>Results: </strong>One hundred and seventy-seven (56.9%) were males and 103 (33%) were belonged to the age group of 12-59 months. The most prevalent causes of hospitalization were status epilepticus (12.9%) and pneumonia (11.2%). Mortality rate was 12.2%. The significant factors associated with mortality were readmission and history of resuscitation. PRISM-III index showed a significant difference between nonsurvivors and survivors (7.05 ± 6.36 vs. 3.36 ± 4.34, <i>P</i> = 0.001). Complications like AKI, hypoglycemia, MODS and disseminated intravascular coagulation (DIC), length of mechanical ventilation significantly correlated with mortality.</p><p><strong>Conclusions: </strong>Mortality rate was less than that of other developing countries (12.2%) and this was associated with some risk factors included readmission, history of resuscitation, PRISM-III Index; complications like AKI, acute respiratory distress syndrome (ARDS), DIC, mechanical ventilation duration, MODS, hypoglycemia, and P-SOFA index.</p>","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"92"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/82/57/ABR-12-92.PMC10241639.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9591428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atorvastatin and Flaxseed Effects on Biochemical Indices and Hepatic Fat of NAFLD Model in Rats.","authors":"Zahra Eslami,&nbsp;Abdorreza E Moghanlou,&nbsp;Yahya M N P Kandi,&nbsp;Mehdi S Arabi,&nbsp;Alireza Norouzi,&nbsp;Hamidreza Joshaghani","doi":"10.4103/abr.abr_21_22","DOIUrl":"https://doi.org/10.4103/abr.abr_21_22","url":null,"abstract":"<p><strong>Background: </strong>Non-alcoholic fatty liver disease (NAFLD) is one of the most common forms of chronic liver disease that affects about 25% of the general population. No definitive treatment for NAFLD has been identified yet. The aim was to determine the effect of atorvastatin (ATO) and flaxseed on related indicators of NAFLD-induced fat/fructose-enriched diet (FFD).</p><p><strong>Materials and methods: </strong>Forty male Wistar rats were divided into five groups. NAFLD groups received FFD and carbon tetrachloride (CCl4) to induce NAFLD. After intervention with ATO (10 mg/kg/day) and/or flaxseed (7.5 g/kg/day), liver enzymes and lipid profiles in serum were determined at eight week of interventions.</p><p><strong>Results: </strong>Triglycerides (TG) and cholesterol (CHO) in FFD + ATO, FFD + flaxseed, and FFD + ATO + flaxseed had a significant decrease and low-density lipoprotein (LDL) level and LDL/high-density lipoprotein (HDL) ratio showed a significant increase in the FFD + flaxseed compared to the FFD. The levels of aspartate transaminase (AST), alanine transaminase (ALT), and gamma-glutamyltransferase (GGT) were significantly reduced in the FFD + ATO, FFD + flaxseed, and the FFD + ATO + flaxseed. In addition, Alkaline phosphatase (ALP) levels were significantly different between normal and FFD. Fasting blood sugar (FBS) levels were significantly different in the FFD + flaxseed and the FFD + ATO + flaxseed compared to the FFD.</p><p><strong>Conclusion: </strong>ATO therapy along with flaxseed controls NAFLD-related indices and FBS. Therefore, it can be stated with caution that ATO and flaxseed can be used to improve lipid profile and reduce the complications of NAFLD.</p>","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"98"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/58/b5/ABR-12-98.PMC10241636.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9597306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toll-Like Receptor 7 a Novel Non-Invasive Inflammatory Genetic Sensor for Ulcerative Colitis Remission Monitoring.","authors":"Hamid Asadzadeh-Aghdaei,&nbsp;Leili Rejali,&nbsp;Mahyar Nourian,&nbsp;Vahid Chaleshi,&nbsp;Naghmeh Zamani,&nbsp;Shaghayegh Baradaran-Ghavami,&nbsp;Mohsen Nemati,&nbsp;Shabnam Shahrokh,&nbsp;Mohsen Norouzinia,&nbsp;Massoud Vosough,&nbsp;Ehsan Nazemalhosseini-Mojarad,&nbsp;Mohammadreza Zali","doi":"10.4103/abr.abr_24_22","DOIUrl":"https://doi.org/10.4103/abr.abr_24_22","url":null,"abstract":"<p><strong>Background: </strong>Ulcerative colitis (UC) and Crohn's disease (CD) are two major types of inflammatory bowel diseases (IBDs). Toll-like receptors (TLRs) are expressed in the innate immune system compartments, in charge of identifying a wide range of microorganisms. The aim of the present study was to evaluate the expression of <i>TLR-2</i>, -<i>7</i>, and -<i>8</i> in peripheral blood mononuclear cells (PBMC) of UC patients as a novel non-invasive primary inflammation sensor for monitoring the clinical course of UC candidates.</p><p><strong>Materials and methods: </strong>In this cross-sectional study, total RNA was extracted from the PBMC of 42 UC patients along with 20 healthy donors. The mRNA levels of <i>TLR-2</i>, -<i>7</i>, and -<i>8</i> were assessed using the quantitative real-time polymerase chain (qRT-PCR) reaction.</p><p><strong>Results: </strong>The present research study demonstrated no significant changes in <i>TLR-2</i> mRNA expression in UC patients in comparison with the control group (<i>P</i> = 0.1264), whereas significant elevation (<i>P</i> = 0.0008) was distinguished in the <i>TLR-7</i> expression of UC participants specifically during the remission course compared with healthy donors and flareup patients (<i>P</i> = 0.0004 and <i>P</i> = 0.0063, respectively). The last selected TLR, <i>TLR-8</i> was not shown remarkable changes either between UC patients and the control group or between clinical courses of the disease.</p><p><strong>Conclusion: </strong>Here, among three nominated TLRs for predicting UC patients, <i>TLR</i>-7 was potentially selected according to the significant difference in mRNA expression in flareup UC patients and control donors. <i>TLR</i>-7 could be used as a novel non-invasive biomarker for monitoring UC patients in the active course of the disease.</p>","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"54"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cf/4f/ABR-12-54.PMC10086669.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9660731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of Hospitalized COVID-19 Patients in a Major Referral Center in Shiraz, Iran.","authors":"Razieh Dowran,&nbsp;Fahime Edalat,&nbsp;Majid Fardi,&nbsp;Seyed Mohammad Ali Hashemi,&nbsp;Afagh Moattari","doi":"10.4103/abr.abr_399_21","DOIUrl":"https://doi.org/10.4103/abr.abr_399_21","url":null,"abstract":"<p><strong>Background: </strong>Several countries, including Iran, have been affected by the novel Coronavirus Disease 2019 (COVID-19) pandemic since December 2019. The aim of this study was to provide a comprehensive report on COVID-19 patients in Shiraz, Southern Iran.</p><p><strong>Materials and methods: </strong>This study was performed on 311 hospitalized patients with COVID-19. The data on demographic, clinical, and paraclinical features were analyzed.</p><p><strong>Results: </strong>The median age of the patients was 58 years, with 42.1% of the patients being above 60 years of age. Upon admission, fever was detected in 28.2% of critically ill patients. At least one underlying disease or risk factor was also present in 75.6% of the patients. Shortness of breath was the most common clinical symptom (66.2%), dry cough (53.7%), and muscle pain (40.5%) was the second and third. Sneezing (0.3%), rhinorrhea (0.7%), and sore throat (3.09%) were observed only in non-critically ill patients. In addition, 26.9% of all patients had lymphocytopenia, 25.8% had raised C-reactive protein, and 79.9% had abnormal creatinine levels. Finally, death occurred in 39 patients (12.5%).</p><p><strong>Conclusions: </strong>Noncritically ill patients were younger than critically ill patients. The most common risk factors for getting critically ill were surgery, hypertension, diabetes mellitus, chronic heart disease, asthma, and chronic renal disease.</p>","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"137"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0f/40/ABR-12-137.PMC10331542.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10172599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Opportunistic Fungi from the Bronchoalveolar Lavage Specimens of Patients with Pulmonary Diseases.","authors":"Sahar Kianipour,&nbsp;Parvin Dehghan,&nbsp;Mohammad Emami Ardestani","doi":"10.4103/abr.abr_297_22","DOIUrl":"https://doi.org/10.4103/abr.abr_297_22","url":null,"abstract":"<p><strong>Background: </strong>Opportunistic fungi are a constantly evolving group of pathogens that become active when the immune system is compromised, begin to multiply, and soon overwhelm the weakened immune system. This study was performed to evaluate the number of opportunistic fungi in bronchoalveolar lavage (BAL) samples of patients with pulmonary diseases.</p><p><strong>Materials and methods: </strong>After receiving patients' consent and demographic forms, a total of 120 BAL samples were taken by a pulmonary physician. The etiologic agents were identified by standard morphological and molecular methods. Yeast cells were counted on culture media, and direct smears were precisely examined for the presence of yeasts elements, <i>Pneumocystis</i>, and filamentous fungi.</p><p><strong>Results: </strong>In this study, 29 (24.1%) patients showed positive direct smears for yeast elements in their BAL samples. The mean colony count of yeasts was 42,000 (CFU/mL) on culture media. Six (5%) species of filamentous fungi, including three (2.5%) isolates of <i>Penicillium</i> species (<i>P. variabile, P. glabrum</i>, and <i>P. thomii</i>), two (1.67%) <i>Aspergillus</i> species (<i>A. flavus</i> and <i>A. fumigatus</i>), 1 case (0.83%) <i>Pseudallescheria</i> boydii were detected. Seven cases (5.83%) of <i>Pneumocystis</i> cysts were observed in the direct smears stained with Giemsa. Identification of all fungi confirmed by molecular or sequencing methods.</p><p><strong>Conclusions: </strong>Due to the presence of a large number of fungi in the BAL samples and possible physical interference with the selected drugs for treatment, we draw the attention of pulmonologists to this important issue. Rapid diagnosis of fungal infections is essential to optimize treatments and outcomes.</p>","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"176"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/21/0f/ABR-12-176.PMC10492593.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10213720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Biochemical, Haematological and Plasmatic Butyrylcholinesterase Parameters in Farmers and Non-Farmers, Morocco.","authors":"Hasnaa Sine,&nbsp;Youssef Bouchriti,&nbsp;Hayat Sine,&nbsp;Abderrahmane Achbani","doi":"10.4103/abr.abr_370_22","DOIUrl":"https://doi.org/10.4103/abr.abr_370_22","url":null,"abstract":"<p><strong>Background: </strong>The long-term use of pesticides can cause harmful consequences to both human health and the environment. In the present research, we aimed to compare biochemical, hematological, and plasmatic measurements of butyrylcholinesterase (BChE) between farmers and non-farmers.</p><p><strong>Materials and methods: </strong>The study is cross-sectional and included 270 participants, with 135 farmers using pesticides and a control population of 135 non-farmers. The recruitment of the participants was conducted from August 2017 to the end of December 2019. Blood samples from participants were collected for the evaluation of biochemical markers of the function of the liver and determination of BChE activity. A whole blood sample with ethylenediamine tetraacetic anticoagulant (EDTA) was also taken for a complete blood count.</p><p><strong>Results: </strong>The results showed a statistically significant (<i>P</i> = 0.03) decrease in mean corpuscular hemoglobin (MCH) in the cases (28.45 ± 2.94 pg) as compared with controls (29.17 ± 2.54 pg). The statistical analysis of the renal parameters between the two groups determined that the uremia value was significantly higher in cases (34 ± 12 mg/dL) when compared to the control group (29 ± 8 mg/dL) <i>P</i> < 0.001. The cases recorded a significant increase in aspartate aminotransferase (AST) (26.22 ± 11.59 U/L) and alanine aminotransferase (ALT) (25.63 ± 13.47 U/L) enzyme activities among cases versus controls. The results obtained showed a significantly decreased BChE activity in the group of cases exposed to pesticides (7554.52 ± 2107 U/l) compared to the unexposed control group (10135.58 ± 1909 U/l) (<i>t</i>-test, <i>P</i> < 0.001).</p><p><strong>Conclusion: </strong>The education of the farmers on correct practices concerning phytosanitary use has the potential of reducing their exposure to these products.</p>","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"181"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a0/ca/ABR-12-181.PMC10492594.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10222226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Effect of Aripiprazole Supplementation in the Prevention of Delirium in Patients Admitted to the General Intensive Care Unit.","authors":"Mehranoosh Nouri,&nbsp;Sohrab Salimi,&nbsp;Mohsen Kosha,&nbsp;Dariush Abtahi","doi":"10.4103/abr.abr_314_21","DOIUrl":"https://doi.org/10.4103/abr.abr_314_21","url":null,"abstract":"Background: To prove the position of aripiprazole as a preventive and safe agent in delirium in patients admitted to the intensive care unit (ICU), it is necessary to conduct randomized controlled clinical trials with appropriate design. Materials and Methods: In this study, 80 patients were randomly divided into two groups of 40. Group A received placebo mart for one week, and group B received 15 mg aripiprazole daily (dissolved in 10 cc) daily. As a criterion for assessing delirium, the Confusion Assessment methods for the ICU (CAM-ICUs) were evaluated daily for patients. At the end of the study, the trends of CAM-ICU and CAM-ICU changes on day 7 were compared between the two groups. All statistical tests were performed in two domains with a significance level of 5% using the t-test. Statistical Package for the Social Sciences (SPSS) 21 software was used to analyze the data. Results: The use of aripiprazole in the studied indices was not statistically significant (P > 0.05). The results related to the length of stay in the ICU, showed that although the use of aripiprazole has reduced the length of hospitalization of patients in the ICU, this rate of reduction, was not significant (P > 0.05). Conclusion: The use of aripiprazole with the approach of reducing the risk or controlling the occurrence of delirium on patients admitted to the intensive care unit, despite creating some beneficial effects such as reducing the length of hospital stay in the ICU, cannot be clearly and significantly effective.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"198"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7a/54/ABR-12-198.PMC10492625.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10223597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Preventive Effects of Neostigmine Plus Atropine on Post-Dural Puncture Headache.","authors":"Zahra Ahmadzade,&nbsp;Mohammad Golparvar,&nbsp;Shahram Sepiani","doi":"10.4103/abr.abr_81_22","DOIUrl":"https://doi.org/10.4103/abr.abr_81_22","url":null,"abstract":"<p><strong>Background: </strong>Post-dural puncture headache (PDPH) is one of the most common side effects of spinal anesthesia. Several strategies and drugs have been suggested for the treatment and/or prevention of this headache. The aim of this study is evaluating the effects of intravenous prescription of neostigmine plus atropine 15 minutes after dural puncture on incidence and severity of PDPH during 5 days of follow-up in the setting of lower limb orthopedic surgeries.</p><p><strong>Materials and methods: </strong>In a randomized, controlled, double-blind clinical trial, 99 patients of lower limb orthopedic surgeries were randomized into study (49 patients) and control groups (50 patients). Fifteen minutes after dural puncture, participants in the two groups intravenously took neostigmine (40 μg/kg) plus atropine (20 μg/kg) and placebo (normal saline), respectively. Side effects of the studied drugs and incidence, severity, and duration of PDPH were evaluated 5 days after surgery.</p><p><strong>Results: </strong>A total of 20 patients in the study group and 31 in the control group showed a headache-with-PDPH profile during 5 days of follow-up (<i>P</i>-value = 0.035). The mean duration of PDPH was 1.15 ± 0.48 and 1.32 ± 0.54 days in the study and control groups, respectively (<i>P</i>-value = 0.254).</p><p><strong>Conclusion: </strong>Preventive administration of 40 μg/kg neostigmine plus 20 μg/kg of atropine may be effective in reducing the incidence and severity of PDPH after spinal anesthesia in lower limb orthopedic surgeries.</p>","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"119"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7b/97/ABR-12-119.PMC10331525.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9812367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Limb-Girdle Muscular Dystrophy Type 2B (LGMD2B) caused by Pathogenic Splice and Missense Variants of <i>DYSF</i> Gene among Iranians with Muscular Dystrophy.","authors":"Fatemeh Arab,&nbsp;Najmeh Ahangari,&nbsp;Hadis Malek,&nbsp;Mohammad Doosti,&nbsp;Paria Najarzadeh Torbati,&nbsp;Ehsan Ghayoor Karimiani","doi":"10.4103/abr.abr_131_22","DOIUrl":"https://doi.org/10.4103/abr.abr_131_22","url":null,"abstract":"<p><strong>Background: </strong>The phenotypic range of limb-girdle muscular dystrophies (LGMDs) varies significantly because of genetic heterogeneity ranging from very mild to severe forms. Molecular analysis of the <i>DYSF</i> gene is challenging due to the wide range of mutations and associated complications in interpretations of novel <i>DYSF</i> variants with uncertain significance. Thus, in the current study, we performed the NGS analysis and its results are confirmed with Sanger sequencing to find the plausible disease-causing variants in patients with muscular dystrophy and their relatives via segregation analysis.</p><p><strong>Materials and methods: </strong>Nine patients with LGMD type 2B (LGMD2B) characteristics were screened for putative mutations by the whole-exome sequencing (WES) test. Either the patients themselves or their parents and first relatives were investigated in the segregation analysis through Sanger sequencing. The majority of variants were classified as pathogenic through American College of Medical Genetics and Genomics (ACMG) guidelines, segregation results, and <i>in silico</i> predictions.</p><p><strong>Results: </strong>Results revealed eight variants in <i>DYSF</i> gene, including three splicing (c.1149+4A>G, c.2864+1G>A, and c.5785-7G>A), two nonsense (p.Gln112Ter and p.Trp2084Ter), two missense (p.Thr1546Pro and p.Tyr1032Cys), and one frameshift (p.Asp1067Ilefs), among nine Iranian families. One of the eight identified variants was novel, including p.Asp1067Ilefs, which was predicted to be likely pathogenic based on the ACMG guidelines. Notably, prediction tools suggested the damaging effects of studied variants on dysferlin structure.</p><p><strong>Conclusion: </strong>Conclusively, the current report introduced eight variants including a novel frameshift in <i>DYSF</i> gene with noticeable pathogenic effects. This study significantly can broaden the diagnostic spectrum of LGMD2B in combination with previous reports about <i>DYSF</i> mutations and may pave the way for a rapidly high-ranked identification of the accurate type of dysferlinopathy.</p>","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"150"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ad/38/ABR-12-150.PMC10410417.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9969659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cisplatin-Resistant Ovarian Cancer Cells Reveal a Polyploid Phenotype with Remarkable Activation of Nuclear Processes.","authors":"Rezvan Adibi,&nbsp;Shiva Moein,&nbsp;Yousof Gheisari","doi":"10.4103/abr.abr_348_21","DOIUrl":"https://doi.org/10.4103/abr.abr_348_21","url":null,"abstract":"<p><strong>Background: </strong>Tumor recurrence as one of the main causes of cancer death is a big barrier to cancer complete treatment. Various studies denote the possible role of therapeutics in tumor relapse. Cisplatin as one of the generally used chemotherapy agents is supposed to be the source of therapy resistance through formation of polyploid giant cancer cells (PGCCs). Nevertheless, the mechanisms by which PGCCs promote tumor relapse are not fully understood.</p><p><strong>Materials and methods: </strong>In this study, we performed experimental and bioinformatic investigations to recognize the mechanisms related to cisplatin resistance. A2780 and SCOV-3 cell lines were treated with cisplatin for 72 hours and were evaluated for their morphology by fluorescent microscopy and DNA content analysis. Furthermore, a microarray dataset of cisplatin-resistant ovarian cancer cells was re-analyzed to determine the significantly altered genes and signaling pathways.</p><p><strong>Results: </strong>Although cisplatin led to death of considerable fraction of cells in both cell lines, a significant number of survived cells became polyploid. On the other hand, our high throughput analysis determined significant change in expression of 1930 genes which mainly related to gene regulatory mechanisms and nuclear processes. Besides, mTOR, hypoxia, Hippo, and 14-3-3 signaling pathways previously shown to have role in PGCCs were determined.</p><p><strong>Conclusion: </strong>Taken together, results of this study demonstrated some key biological mechanisms related to cisplatin-resistant polyploid cancer cells.</p>","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"77"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/59/d4/ABR-12-77.PMC10186044.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10645374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}