American journal of clinical and experimental immunology最新文献

{"title":"New strategy against sperm oxidative damage: supplementing NaHS to enhance the activity of the H₂S antioxidant pathway mediated by E3 ubiquitin ligase ASB1.","authors":"Nianchao Zhou, Mingxi Liu, Xuejun Shang","doi":"10.62347/LZHL6737","DOIUrl":"10.62347/LZHL6737","url":null,"abstract":"","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":"14 1","pages":"43-44"},"PeriodicalIF":1.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11932064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PYGO2 as a novel prognostic biomarker and its correlation with immune infiltrates in liver cancer.","authors":"Jieyu Jin, Yanqiu Zhang, Jun Cao, Junchao Feng, Yuting Liang, Longwei Qiao, Bin Feng, Qingqin Tang, Jun Qiu, Zhongping Qian","doi":"10.62347/RSAT7482","DOIUrl":"10.62347/RSAT7482","url":null,"abstract":"<p><strong>Objective: </strong>The PYGO2 gene plays a significant role in various cancers. However, its prognostic significance and involvement in immune infiltration in liver cancer remain unclear. This study aimed to comprehensively evaluate PYGO2 expression and its associations with prognosis and clinicopathological features in liver cancer.</p><p><strong>Methods: </strong>Data from The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases were analyzed. Functional enrichment analysis and immune cell infiltration assessments were performed to explore potential pathogenic mechanisms.</p><p><strong>Results: </strong>PYGO2 was highly expressed in multiple cancer types, including bladder urothelial carcinoma, breast invasive carcinoma, cholangiocarcinoma, diffuse large B-cell lymphoma, and liver cancer. Analysis of 50 paired liver cancer tissues from TCGA revealed significant upregulation of PYGO2 expression. Moreover, high PYGO2 expression was significantly associated with pathological T stage, overall pathological stage, tumor status, and race. Kaplan-Meier survival analysis showed that low PYGO2 expression correlated with improved overall survival (OS), disease-specific survival (DSS), and progression-free interval (PFI) in liver cancer patients. Functional enrichment analysis identified several enriched pathways, including the reactive oxygen species signaling pathway, MYC targets, interferon-alpha response, immune response regulation signaling pathway, and leukocyte migration. Additionally, PYGO2 overexpression was associated with lower proportions of cytotoxic cells, dendritic cells, immature dendritic cells, mast cells, neutrophils, plasmacytoid dendritic cell-like cells, Th17 cells, and regulatory T cells, but a higher proportion of Th2 cells. Furthermore, the high PYGO2 expression group exhibited increased immune checkpoint gene expression, particularly PDCD1.</p><p><strong>Conclusion: </strong>PYGO2 is a promising prognostic biomarker for liver cancer, given its strong associations with clinicopathological features, survival outcomes, and immune-related characteristics.</p>","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":"14 1","pages":"23-33"},"PeriodicalIF":1.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11932060/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decreased IL-10R-expressing B Cells in breast cancer patients: a potential biomarker for early cancer detection.","authors":"Faezeh Absalan, Fereshteh Mehdipour, Mahmoud Shariat, Abdoul-Rasoul Talei, Abbas Ghaderi","doi":"10.62347/VYQY9361","DOIUrl":"10.62347/VYQY9361","url":null,"abstract":"<p><p>IL-10 plays a crucial role in regulating B cell function and differentiation, but its effects on B cells depend on its activation state. The frequency of IL-10R⁺ B cells and how it changes during breast cancer progression have not been studied. This study aimed to evaluate the expression of IL-10R on B cells in the peripheral blood of 50 patients with breast cancer compared to 29 healthy controls. After isolating mononuclear cells, we stained them using anti-CD19 and anti-IL-10R antibodies. We used Flow cytometry to analyze the expression of IL-10R on B cells. We found that over 50% of B cells in both patients with breast cancer and healthy controls expressed IL-10R. However, patients had a significantly lower frequency of IL-10R⁺ B cells compared to healthy individuals (64±16.0% of patients compared to 78.5±6.6% for healthy individuals, P<0.0001). This decrease was not associated with lymph node involvement or tumor size. Phenotypic analysis revealed that IL-10R-expressing B cells consist of both naive and memory B cells, with the majority of peripheral memory B cells expressing IL-10R. The decrease in IL-10R-expressing B cells in breast cancer patients may be due to apoptosis induced by IL-10 in naive or recently activated B cells, or their migration to lymph nodes to combat tumor cells. Our study suggests that IL-10R could be a potential biomarker for detecting breast cancer in its early stages.</p>","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":"14 1","pages":"34-42"},"PeriodicalIF":1.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11932062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of immune infiltration-related ZNF480 for predicting prognosis in breast cancer.","authors":"Tong Zhou, Yanqiu Zhang, Jun Cao, Qingqin Tang, Yuting Liang, Sheng Zhang, Mengyuan Hu, Bin Feng, Jieyu Jin","doi":"10.62347/LNKO2367","DOIUrl":"10.62347/LNKO2367","url":null,"abstract":"<p><strong>Background: </strong>Breast cancer is one of the most common cancers in women with high morbidity and mortality. ZNF480, a member of the KRAB-ZNFs family, correlates with cancer progression. However, its role in the development and progression of breast cancer remains unclear.</p><p><strong>Methods: </strong>We utilized transcriptomic and clinical data from The Cancer Genome Atlas (TCGA) and Genotype Tissue Expression (GTEx) databases of breast cancer patients to investigate the potential pro-cancer role of ZNF480, including differential expression of ZNF480 in breast cancer, prognostic value, clinicopathological features, immune cell infiltration relevance and function enrichment analysis.</p><p><strong>Results: </strong>Our results indicate that ZNF480 is upregulated in breast cancer and is correlations with survival, clinical stage, race and tumor subtype in breast cancer patients. Additionally, immune infiltration analysis revealed significant negative correlations between ZNF480 expression and multiple tumor infiltrating immune cells, including aDC, B cells, CD8 T cells, Cytotoxic cells, DC, iDC, Macrophages, Neutrophils, NK CD56bright cells, NK CD56dim cells, NK cells, pDC, T cells, Tem, TFH and Th1 cells, whereas a significant positive correlation was observed with the infiltration of T helper cells, Tcm, Tgd and Th2 cells. Furthermore, functional enrichment analysis indicated that ZNF480 may be involved in Angiogenesis, Allograft rejection, TNFα signaling via NFκB, Coagulation, IL6 Jak STAT3 signaling, Inflammatory response, Interferon gamma response and other processes.</p><p><strong>Conclusion: </strong>ZNF480 is highly expressed in breast cancer and correlates with immune cell infiltration, and may be a candidate prognostic biomarker, which may assist in breast cancer treatment.</p>","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":"14 1","pages":"1-13"},"PeriodicalIF":1.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11932063/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The prognostic significance of FMR1 expression and its immunomodulatory implications in esophageal carcinoma.","authors":"Qingqin Tang, Yanqiu Zhang, Yuting Liang, Jun Qiu, Sheng Zhang, Jieyu Jin, Jun Cao, Longwei Qiao, Bin Feng","doi":"10.62347/XVFP6530","DOIUrl":"10.62347/XVFP6530","url":null,"abstract":"<p><strong>Background: </strong>Esophageal carcinoma (ESCA) is deemed a highly lethal malignancy with a grim prognosis and stands as the fourth leading cause of cancer-related mortality. Recent research has revealed the potential crucial role of fragile X mental retardation 1 (FMR1) protein in tumor development and progression. However, the correlation between FMR1 and immune regulation in ESCA remains unclear. In this study, we aimed to assess the clinicopathological and prognostic significance of FMR1 expression, and its relationship with immune cell infiltration, immune biomarkers and the pathway involved in ESCA.</p><p><strong>Methods: </strong>The Cancer Genome Atlas (TCGA) pan-cancer data and the Gene Expression Omnibus (GEO) database were used to analyze the expression of FMR1. The correlation between FMR1 and cancer stage, time-dependent survival curve and receiver operating characteristic (ROC) curve were performed using R package. Immune cell infiltration was assessed using the samples found in TCGA. Functional enrichment analyses were performed to investigate the potential signaling pathway and biological functions.</p><p><strong>Results: </strong>FMR1 was upregulated in 7 tumors and downregulated in 4 tumors. Overexpression of FMR1 considerably associated with cancer stage and poor prognosis in ESCA. The ROC area was 0.745 and 0.830 for 3-year and 5-year respectively. FMR1 exhibited a positive correlation with common lymphoid progenitor and T cell CD4+ Th2, and a negative correlation with B cell memory, B cell plasma, endothelial cell, monocyte, neutrophil, T cell CD4+ Th1, and T cell CD4+ effector memory in ESCA. The enrichment analysis revealed FMR1 was primarily associated with cell development and predominantly enriched in immune-related pathways.</p><p><strong>Conclusion: </strong>FMR1 may act as a prognostic biomarker for ESCA and participate in immune regulation in ESCA.</p>","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":"14 1","pages":"14-22"},"PeriodicalIF":1.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11932061/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endocannabinoid and hematological responses to pre- and post-therapeutic exercises in liver transplant patients.","authors":"Abdullah Nasser AlShahrani, Thamir Al-Khlaiwi, Sultan Ayoub Meo, Intisar Ahmad Siddiqui, Bandar Alghanem, Feras Almourfi","doi":"10.62347/FNLX9490","DOIUrl":"10.62347/FNLX9490","url":null,"abstract":"<p><p>Endocannabinoids (eCBs) play a crucial role in regulating the pathophysiological progression of chronic liver disease through hepatic cannabinoid receptor 2 (CB2). According to the literature, various treatment options are available for liver disease patients, including transplantation and physical activity both before and after the procedure. The aim of this study is to assess the response of endocannabinoids to pre- and post-therapeutic exercises in liver transplant patients (LTx). This analytical case-control longitudinal study was conducted on patients aged 18-70 at King Fahad Specialist Hospital in Dammam, Saudi Arabia. Participants were divided into two groups: an intervention group of LTx patients (n = 26) and a control group of end-stage liver disease patients (n = 23) who were not candidates for liver transplantation (LT). Blood samples were collected before the initiation of preoperative exercises, one month before LT, and three months after LT following postoperative exercises. The median arachidonoyl ethanolamide (AEA) levels in the control group were comparatively higher after therapeutic exercises compared to before; however, the Wilcoxon signed-rank test showed no significant differences (P = 0.212). In the LTx group, the median difference in AEA between pre- and post-therapeutic exercises was marginally significant (P = 0.091). Additionally, the Wilcoxon signed-rank test revealed a highly significant increase in median 2-arachidonoylglycerol (2-AG) levels after therapeutic exercises compared to before in the LTx group (P = 0.049), while the control group showed no significant change in post- vs. pre-therapeutic exercise median 2-AG levels (P = 0.346). The study's findings revealed an increased concentration of 2-AG after therapeutic exercises in LTx patients but not in the control group, while AEA levels were elevated after therapeutic exercises in both groups. The effect of post-therapeutic exercises on hematological and biochemical markers was significant between the control and LTx groups, particularly concerning platelet count, total bilirubin, total protein, albumin/globulin ratio, international normalized ratio, and calcium levels.</p>","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":"13 6","pages":"259-271"},"PeriodicalIF":1.4,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744344/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Observation on the therapeutic effect of rolling the target muscle groups of lower limbs with foam rollers of different shore hardness on DOMS.","authors":"Yutong Lu, Wenhui Xue, Renxin Ji","doi":"10.62347/BUIX3741","DOIUrl":"10.62347/BUIX3741","url":null,"abstract":"<p><strong>Objective: </strong>This experiment aims to explore how foam rollers of different Shore hardness affect DOMS, providing insights for sports therapy.</p><p><strong>Methods: </strong>Forty participants from Shanghai Sanda University who have no habit of strength training, no lower limb injury, and meet the health standards were selected to conduct three experiments under the conditions of no intervention, using a 50 Shore hardness foam roller, and using a 60 Shore hardness foam roller, respectively. Data were recorded before and after modeling, as well as 24, 48, and 72 hours later.</p><p><strong>Results: </strong>There were no significant differences in various indicators among the three groups of subjects before and immediately after DOMS modeling (<i>P</i>>0.05). Following intervention, the 60 Shore hardness foam roller significantly reduced DOMS pain (NRS score) compared to the 50 Shore hardness roller, improved knee flexion range of motion, and increased standing long jump distance (<i>P</i><0.05).</p><p><strong>Conclusions: </strong>The 60 Shore hardness foam roller is superior to the 50 Shore hardness foam roller in alleviating DOMS, improving joint range of motion, and enhancing athletic performance.</p>","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":"13 6","pages":"272-277"},"PeriodicalIF":1.4,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744345/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tertiary lymphoid structures in colorectal cancer - organization and immune cell interactions.","authors":"Maya Vladova Gulubova, Stefan P Valkanov, Maria Magdalena K Ignatova, Georgi A Minkov","doi":"10.62347/GRYY2849","DOIUrl":"10.62347/GRYY2849","url":null,"abstract":"<p><p>Tertiary lymphoid structures (TLS), formerly recognized as Crohn's-like structures, serve as crucial biomarkers for evaluating the progression of colorectal cancer (CRC). Understanding their spatial distribution, cellular composition, and interactions within CRC is paramount for comprehending the immune response in the tumor microenvironment (TME). TLS are comprised of a T-cellular compartment and a B-cellular compartment, the latter encompassing follicular dendritic cells (FDCs), high endothelial venules (HEVs), and lymphatic vessels. While T helper cells predominate in cancer TLS, the specific functions of their subpopulations remain inadequately understood. Notably, T follicular helper (Tfh) cells play a central role in the activation of CD8⁺ T cells, and both Tfh cells and Tfh-associated genes have been linked to enhanced CRC survival. In stage II CRC TLS, an escalation in the number of FoxP3⁺ T regulatory cells (Tregs) is regarded as a negative prognostic factor. Moreover, within TLS, T lymphocytes shield B lymphocytes from the immunosuppressive effects of the TME. B lymphocyte activation is succeeded by class recombination (CSR) and somatic hypermutation (SHM). Dendritic cells (DCs) constitute a vital cellular component of the TLS T compartment. During steady state and early stages of CRC, specialized antigen-presenting cells such as DCs migrate to regional lymph nodes through afferent lymphatics. They deliver MHC antigen-derived peptide complexes (tumor antigens) to naïve CD4⁺ and CD8⁺ T cells, which subsequently infiltrate the tumor site as antigen-specific T cells. Key DC markers studied in TLS include CD83 and DC-LAMP. Research has indicated that the DC-LAMP gene signature in tumor TLS reflects Th1 cell targeting, cytotoxicity, and T cell activation. This review comprehensively outlines the functions performed by distinct cell subsets within tertiary lymphoid structures (TLS) in tumors.</p>","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":"13 6","pages":"236-245"},"PeriodicalIF":1.4,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744347/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat shock protein 70 gene polymorphisms in Iranian patients with Multiple sclerosis.","authors":"Azam Bakhshandeh, Alireza Kargar Dolatabadi, Touraj Farazmanfar, Majid Shahbazi","doi":"10.62347/CMYA9839","DOIUrl":"10.62347/CMYA9839","url":null,"abstract":"<p><p>Genetic factors are effective reagents in susceptibility to multiple sclerosis (MS). Previous studies have shown the relationship between heat shock protein (HSP) gene polymorphisms. So, HSP70 single nucleotide polymorphisms (SNPs) were evaluated as MS risk factors. Here, DNA genotyping was done for HSP70 gene polymorphisms, including HSP70-1 +190 G>C, HSP70-1 -110 A>C, HSP70-1 +438 A>C, and HSP70-hom +2437 A>G in two groups including Iranian MS patients and controls. A standard phenol/chloroform method isolated DNA samples from peripheral blood. Sequence-specific amplification (SSP) polymerase chain reaction (PCR) was used for genotyping polymorphisms. Overall, 76 (35.80%) MS patients and 136 (65.10%) controls were studied with an age mean of 36.0 ± 8.0 years. Female/male was significantly higher in patients than in controls (4.43 vs. 0.10, <i>P</i> < 0.001). The average age was significantly lower in patients (<i>P</i> < 0.001). The most common clinical feature was relapsing-remitting (RR) MS; more than half of the population was Fars. Results showed that genotypes of HSP70-hom +2437 C>T had a significant relation with MS (OR = 2.0, 95% CI = 1.0-5.0, P = 0.03) and the same applies to HSP70-1 -110 A>C (OR = 0.0, 95% CI = 0.0-1.0, P < 0.001). Allele and genotype frequency of two other HSP70 SNPs (HSP70-1 +190 G>C, HSP70-1 +438 A>C) showed no significant differences between patients and controls. HSP70-hom +2437 C>T and HSP70-1 -110 A>C can be considered as risk factors for MS in our population. However, other HSP SNPs should be studied in a larger population in the future.</p>","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":"13 6","pages":"278-284"},"PeriodicalIF":1.4,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744348/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting candidate biomarkers for COVID-19 associated with leukemia in children.","authors":"Judy Bai, Qing Li","doi":"10.62347/ULTA9461","DOIUrl":"10.62347/ULTA9461","url":null,"abstract":"<p><p>Since the COVID-19 pandemic, a significant number of pediatric leukemia patients have shown to have also contracted COVID-19 several weeks or months prior to the development of their cancer. Current research indicates the expression of MDA5, encoded by <i>IFIH1</i>, is associated with increased immunity to COVID-19 in children. Children are also known to have a much lower risk of developing leukemia. Our hypothesis is that <i>IFIH1</i> and its regulatory miRNAs are biomarkers associated with pediatric leukemia; the objective of our study is to identify genes, through miRNA targeting mechanisms, which may be biomarkers associated with COVID-19 infection and leukemia. The database TarBase was analyzed to identify miRNAs that target <i>IFIH1</i>, followed by the identification of other genes regulated by <i>IFIH1</i>'s targeting miRNAs, to construct a gene-miRNA targeting network. Protein-Protein Interaction (PPI) analysis and DAVID/KEGG pathway analysis were conducted to identify genes with meaningful biological interactions and pathways. We identified two significant miRNAs, <i>hsa-196a-5p</i> and <i>hsa-196b-5p</i>, and 51 of their targeted and highly expressed genes reported in the Acute Myeloid Leukemia (AML) samples from The Cancer Genome Atlas (TCGA) RNA sequencing database. When conducting additional analysis using the Gene Constellation module of the Immunological Genome Project for the top three candidate genes, several other genes were identified to be highly correlated with <i>STAT3</i> and <i>IFIH1</i> in our study. Based on our investigation into co-expression analysis, we found that <i>IFIH1</i> is a potential biomarker for AML. We are expanding our work to create a machine learning model to identify other biomarkers, examine the significance of various parameters (age, race, etc.), and perform comorbidity network analysis for other potential genes/miRNAs.</p>","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":"13 6","pages":"246-258"},"PeriodicalIF":1.4,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744346/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}