Advanced genetics (Hoboken, N.J.)最新文献

{"title":"(Advanced Genetics 1/04)","authors":"","doi":"10.1002/ggn2.202370011","DOIUrl":"10.1002/ggn2.202370011","url":null,"abstract":"","PeriodicalId":72071,"journal":{"name":"Advanced genetics (Hoboken, N.J.)","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10000269/pdf/GGN2-4-2370011.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9086803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Board: (Advanced Genetics 1/04)","authors":"","doi":"10.1002/ggn2.202370012","DOIUrl":"https://doi.org/10.1002/ggn2.202370012","url":null,"abstract":"Nadav Ahituv, University of California, San Francisco, San Francisco, CA USA Nir Barzilai, Albert Einstein College of Medicine, Bronx, NY USA Jacqueline Batley, University of Western Australia, Perth, Australia Touati Benoukraf,Memorial University of Newfoundland, St. John’s, NL, Canada Ewan Birney, EMBL-EBI, Cambridge, UK Catherine A. Brownstein, Boston Children’s Hospital, Boston, MA USA Stephen J. Chanock, National Cancer Institute, Bethesda, MD USA George Church, Harvard Medical School, Boston, MA USA Francesco Cucca, University of Sassari, Sassari, Sardinia, Italy Marcella Devoto, Istituto di Ricerca Genetica e Biomedica, Consiglio Nazionale delle Ricerche, Cagliari, Italy Roland Eils, Berlin Institue of Health, Berlin, Germany Jeanette Erdmann, Institute for Cardiogenetics, University of Lubeck, Lubeck, Germany Andrew Feinberg, Johns Hopkins University, Baltimore, MD USA Claudio Franceschi, University of Bologna, Bologna, Italy Paul W. Franks, Lund University, Malmö, Sweden Rachel Freathy, University of Exeter, Exeter, UK Jingyuan Fu, University Medical Center Groningen, Groningen, The Netherlands Eileen Furlong, European Molecular Biology Laboratory, Heidelberg, Germany Tom Gilbert, University of Copenhagen, The Globe Institute, Copenhagen, Denmark Joseph G. Gleeson, University of California, San Diego, Howard Hughes Medical Institute for Genomic Medicine, La Jolla, CA USA Erica Golemis, Fox Chase Cancer Center, Philadelphia, PA USA Sarah Hearne, International Maize and Wheat Improvement Centre (CIMMYT), Texcoco, Mexico Agnar Helgason, deCODE Genetics, Reykjavik, Iceland Kristina Hettne, Leiden University Libraries, Leiden, The Netherlands Sanwen Huang, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China Youssef Idaghdour, New York University, Abu Dhabi, Abu Dhabi, UAE Rosalind John, Cardiff University, Cardiff, UK Moien Kanaan, Bethlehem University, Bethlehem, Palestine Beat Keller, University of Zurich, Zurich, Switzerland Tuuli Lappalainen, New York Genome Center, Columbia University, New York, NY USA Luis F. Larrondo, Pontifica Universidad Catolica de Chile, Santiago, Chile Suet-Yi Leung, The University of Hong Kong, Hong Kong, China Ryan Lister, The University of Western Australia, Perth, Australia Jianjun Liu, Genome Institute Singapore, Singapore Naomichi Matsumoto, Yokohama City University, Yokohama, Japan Rachel S. Meyer, University of California, Los Angeles, Los Angeles, CA USA Nicola Mulder, University of Cape Town, Cape Town, South Africa Seishi Ogawa, Kyoto University, Kyoto, Japan Guilherme Oliveira, Vale Institute of Technology, Belem, Brazil Qiang Pan-Hammarstrom, Karolinska Institute, Stockholm, Sweden Len A. Pennacchio, Joint Genome Institute, Walnut Creek, CA USA Martin Pera, Jackson Lab, Bar Harbor, ME USA Danielle Posthuma, VU University Amsterdam, Amsterdam, The Netherlands Michael Purugganan, New York University, New York, NY USA Maanasa Raghavan, University of Chic","PeriodicalId":72071,"journal":{"name":"Advanced genetics (Hoboken, N.J.)","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ggn2.202370012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50127642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomics for Clinical and Experimental Biology Research: Hang on a Seq","authors":"Tanner Stokes,&nbsp;Haoning Howard Cen,&nbsp;Philipp Kapranov,&nbsp;Iain J Gallagher,&nbsp;Andrew A. Pitsillides,&nbsp;Claude-Henry Volmar,&nbsp;William E Kraus,&nbsp;James D. Johnson,&nbsp;Stuart M. Phillips,&nbsp;Claes Wahlestedt,&nbsp;James A. Timmons","doi":"10.1002/ggn2.202200024","DOIUrl":"10.1002/ggn2.202200024","url":null,"abstract":"<p>Sequencing the human genome empowers translational medicine, facilitating transcriptome-wide molecular diagnosis, pathway biology, and drug repositioning. Initially, microarrays are used to study the bulk transcriptome; but now short-read RNA sequencing (RNA-seq) predominates. Positioned as a superior technology, that makes the discovery of novel transcripts routine, most RNA-seq analyses are in fact modeled on the known transcriptome. Limitations of the RNA-seq methodology have emerged, while the design of, and the analysis strategies applied to, arrays have matured. An equitable comparison between these technologies is provided, highlighting advantages that modern arrays hold over RNA-seq. Array protocols more accurately quantify constitutively expressed protein coding genes across tissue replicates, and are more reliable for studying lower expressed genes. Arrays reveal long noncoding RNAs (lncRNA) are neither sparsely nor lower expressed than protein coding genes. Heterogeneous coverage of constitutively expressed genes observed with RNA-seq, undermines the validity and reproducibility of pathway analyses. The factors driving these observations, many of which are relevant to long-read or single-cell sequencing are discussed. As proposed herein, a reappreciation of bulk transcriptomic methods is required, including wider use of the modern high-density array data—to urgently revise existing anatomical RNA reference atlases and assist with more accurate study of lncRNAs.</p>","PeriodicalId":72071,"journal":{"name":"Advanced genetics (Hoboken, N.J.)","volume":"4 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ggn2.202200024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10301654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"(Advanced Genetics 4/03)","authors":"","doi":"10.1002/ggn2.202270041","DOIUrl":"10.1002/ggn2.202270041","url":null,"abstract":"","PeriodicalId":72071,"journal":{"name":"Advanced genetics (Hoboken, N.J.)","volume":"3 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9993465/pdf/GGN2-3-2270041.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9099588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global Action on Biodiversity May Hinge on Genetic Data Sharing Agreement","authors":"Jacqueline Batley,&nbsp;Andrew L. Hufton,&nbsp;Guilherme Oliveira,&nbsp;Rajeev K. Varshney","doi":"10.1002/ggn2.202200031","DOIUrl":"10.1002/ggn2.202200031","url":null,"abstract":"<p>This month, parties to the Convention on Biological Diversity (CBD) are meeting in Montreal with the aim of concluding negotiations on an important new action plan for global biodiversity conservation, known as the post-2020 Global Biodiversity Framework (GBF). In these negotiations, genetic data from plants, animals, fungi and microorganisms, known as digital sequence information (DSI) in policy circles, has emerged as a central source of tension. A number of parties are demanding that benefits arising from the use of these genetic data be better shared with the countries where the genetic material was collected.</p><p>The Nagoya Protocol, a component of the CBD, recognized the right of countries to share in the benefits derived from their nation's genetic resources, and established a framework by which countries can regulate and track the use of physical “genetic resources” (i.e., biological samples, strains, plant lines, etc., containing genetic material). This framework, however, is complex and, in the opinion of many, has proven inefficient at driving meaningful benefit sharing.^[<span>¹</span>^]</p><p>Researchers and other stakeholders have raised serious concerns about applying such a framework to DSI.^[<span>^{2, 3}</span>^] Some of the proposals on the table could spell an end to the culture of open sequence sharing that has defined non-human genetics research for decades, and which is widely agreed to have massive positive effects on research progress and economic value creation. A poorly developed solution could therefore have a negative impact on biodiversity research that is crucial to the aims of the CBD. Representatives of indigenous peoples and local communities have also been active in the discussions on this topic and argue that the rights and roles of their communities must be respected in any final agreement.^[<span>⁴</span>^]</p><p>That this issue alone could stymie global biodiversity conservation efforts is not in doubt. Talks in August on a major ocean biodiversity treaty failed to make progress because of lack of agreement on DSI,^[<span>⁵</span>^] and African negotiators have warned that they will not agree to a GBF that lacks a concrete solution to DSI.^[<span>⁶</span>^] The issue has also proven contentious in a recent meeting of the Governing Body session of the International Treaty on Plant Genetic Resources for Food and Agriculture.^[<span>⁷</span>^] It is clear that an effective benefit sharing solution must be part of any global action plan to conserve biodiversity.</p><p>But grounds for optimism remain. Scientists and major research organizations are arguing that it is possible to build a solution that will drive benefit-sharing, protect open science and promote biodiversity conservation.^[<span>^{4, 8, 9}</span>","PeriodicalId":72071,"journal":{"name":"Advanced genetics (Hoboken, N.J.)","volume":"3 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9993466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9455821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Board: (Advanced Genetics 4/03)","authors":"","doi":"10.1002/ggn2.202270042","DOIUrl":"10.1002/ggn2.202270042","url":null,"abstract":"Nadav Ahituv, University of California, San Francisco, San Francisco, CA USA Nir Barzilai, Albert Einstein College of Medicine, Bronx, NY USA Jacqueline Batley, University of Western Australia, Perth, Australia Touati Benoukraf,Memorial University of Newfoundland, St. John’s, NL, Canada Ewan Birney, EMBL-EBI, Cambridge, UK Catherine A. Brownstein, Boston Children’s Hospital, Boston, MA USA Stephen J. Chanock, National Cancer Institute, Bethesda, MD USA George Church, Harvard Medical School, Boston, MA USA Francesco Cucca, University of Sassari, Sassari, Sardinia, Italy Marcella Devoto, Istituto di Ricerca Genetica e Biomedica, Consiglio Nazionale delle Ricerche, Cagliari, Italy Roland Eils, Berlin Institue of Health, Berlin, Germany Jeanette Erdmann, Institute for Cardiogenetics, University of Lubeck, Lubeck, Germany Andrew Feinberg, Johns Hopkins University, Baltimore, MD USA Claudio Franceschi, University of Bologna, Bologna, Italy Paul W. Franks, Lund University, Malmö, Sweden Rachel Freathy, University of Exeter, Exeter, UK Jingyuan Fu, University Medical Center Groningen, Groningen, The Netherlands Eileen Furlong, European Molecular Biology Laboratory, Heidelberg, Germany Tom Gilbert, University of Copenhagen, The Globe Institute, Copenhagen, Denmark Joseph G. Gleeson, University of California, San Diego, Howard Hughes Medical Institute for Genomic Medicine, La Jolla, CA USA Erica Golemis, Fox Chase Cancer Center, Philadelphia, PA USA Sarah Hearne, International Maize and Wheat Improvement Centre (CIMMYT), Texcoco, Mexico Agnar Helgason, deCODE Genetics, Reykjavik, Iceland Kristina Hettne, Leiden University Libraries, Leiden, The Netherlands Sanwen Huang, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China Youssef Idaghdour, New York University, Abu Dhabi, Abu Dhabi, UAE Rosalind John, Cardiff University, Cardiff, UK Moien Kanaan, Bethlehem University, Bethlehem, Palestine Beat Keller, University of Zurich, Zurich, Switzerland Tuuli Lappalainen, New York Genome Center, Columbia University, New York, NY USA Luis F. Larrondo, Pontifica Universidad Catolica de Chile, Santiago, Chile Suet-Yi Leung, The University of Hong Kong, Hong Kong, China Ryan Lister, The University of Western Australia, Perth, Australia Jianjun Liu, Genome Institute Singapore, Singapore Naomichi Matsumoto, Yokohama City University, Yokohama, Japan Rachel S. Meyer, University of California, Los Angeles, Los Angeles, CA USA Nicola Mulder, University of Cape Town, Cape Town, South Africa Seishi Ogawa, Kyoto University, Kyoto, Japan Guilherme Oliveira, Vale Institute of Technology, Belem, Brazil Qiang Pan-Hammarstrom, Karolinska Institute, Stockholm, Sweden Len A. Pennacchio, Joint Genome Institute, Walnut Creek, CA USA Martin Pera, Jackson Lab, Bar Harbor, ME USA Danielle Posthuma, VU University Amsterdam, Amsterdam, The Netherlands Michael Purugganan, New York University, New York, NY USA Maanasa Raghavan, University of Chic","PeriodicalId":72071,"journal":{"name":"Advanced genetics (Hoboken, N.J.)","volume":"3 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ggn2.202270042","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86774406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mendelian Disorders in an Interstitial Cystitis/Bladder Pain Syndrome Cohort","authors":"Elicia Estrella,&nbsp;Shira Rockowitz,&nbsp;Marielle Thorne,&nbsp;Pressley Smith,&nbsp;Jeanette Petit,&nbsp;Veronica Zehnder,&nbsp;Richard N. Yu,&nbsp;Stuart Bauer,&nbsp;Charles Berde,&nbsp;Pankaj B. Agrawal,&nbsp;Alan H. Beggs,&nbsp;Ali G. Gharavi,&nbsp;Louis Kunkel,&nbsp;Catherine A. Brownstein","doi":"10.1002/ggn2.202200013","DOIUrl":"10.1002/ggn2.202200013","url":null,"abstract":"<p>Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic pain disorder causing symptoms of urinary frequency, urgency, and bladder discomfort or pain. Although this condition affects a large population, little is known about its etiology. Genetic analyses of whole exome sequencing are performed on 109 individuals with IC/BPS. One family has a previously reported <i>SIX5</i> variant (ENST00000317578.6:c.472G&gt;A, p.Ala158Thr), consistent with Branchiootorenal syndrome 2 (BOR2). A likely pathogenic heterozygous variant in <i>ATP2A2</i> (ENST00000539276.2:c.235G&gt;A, p.Glu79Lys) is identified in two unrelated probands, indicating possible Darier-White disease. Two private heterozygous variants are identified in <i>ATP2C1</i> (ENST00000393221.4:c.2358A&gt;T, p.Glu786Asp (VUS/Likely Pathogenic) and ENST00000393221.4:c.989C&gt;G, p.Thr330Ser (likely pathogenic)), indicative of Hailey-Hailey Disease. Sequence kernel association test analysis finds an increased burden of rare <i>ATP2C1</i> variants in the IC/BPS cases versus a control cohort (<i>p</i> = 0.03, OR = 6.76), though does not survive Bonferroni correction. The data suggest that some individuals with IC/BPS may have unrecognized Mendelian syndromes. Comprehensive phenotyping and genotyping aid in understanding the range of diagnoses in the population-based IC/BPS cohort. Conversely, <i>ATP2C1, ATP2A2</i>, and <i>SIX5</i> may be candidate genes for IC/BPS. Further evaluation with larger numbers is needed. Genetically screening individuals with IC/BPS may help diagnose and treat this painful disorder due to its heterogeneous nature.</p>","PeriodicalId":72071,"journal":{"name":"Advanced genetics (Hoboken, N.J.)","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ggn2.202200013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9198467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Repetitive DNA is Functional and Encodes Parts of the Non-Coding RNA Repertoire","authors":"James A. Shapiro","doi":"10.1002/ggn2.202200026","DOIUrl":"10.1002/ggn2.202200026","url":null,"abstract":"<p>This is a commentary on the article by Eviatar Nevo and Kexin Li entitled “Sympatric Speciation in Mole Rats and Wild Barley and Their Genome Repeatome Evolution: A Commentary”, published recently in <i>Advanced Genetics</i>.</p>","PeriodicalId":72071,"journal":{"name":"Advanced genetics (Hoboken, N.J.)","volume":"3 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9993471/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9157268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variation of Female Pronucleus Reveals Oocyte or Embryo Chromosomal Copy Number Variations","authors":"Jingwei Yang,&nbsp;Yikang Wang,&nbsp;Chong Li,&nbsp;Wei Han,&nbsp;Weiwei Liu,&nbsp;Shun Xiong,&nbsp;Qi Zhang,&nbsp;Keya Tong,&nbsp;Guoning Huang,&nbsp;Xiaodong Zhang","doi":"10.1002/ggn2.202200001","DOIUrl":"10.1002/ggn2.202200001","url":null,"abstract":"<p>The characteristics of the human pronuclei (PNs), which exist 16–22 h after fertilization, appear to serve as good indicators to evaluate the quality of human oocyte and embryo, and may reflect the status of female and male chromosome composition. Here, a quantitative PN measurement method that is generated by applying expert experience combined with deep learning from large annotated datasets is reported. After mathematic reconstruction of PNs, significant differences are obtained in chromosome-normal rate and chromosomal small errors such as copy number variants by comparing the size of the reconstructive female PN. After integrating the whole procedure of PN dynamics and adjusting for errors that occur during PN identification, the results are robust. Notably, all positive prediction results are obtained from the female propositus population. Thus, the size of female PNs may mirror the internal quality of the chromosomal integrity of the oocyte. Embryos that develop from zygotes with larger female PNs may have a reduced risk of copy number variations.</p>","PeriodicalId":72071,"journal":{"name":"Advanced genetics (Hoboken, N.J.)","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ggn2.202200001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9102527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A CRISPR Path to Finding Vulnerabilities and Solving Drug Resistance: Targeting the Diverse Cancer Landscape and Its Ecosystem","authors":"Benjamin McLean,&nbsp;Aji Istadi,&nbsp;Teleri Clack,&nbsp;Mezzalina Vankan,&nbsp;Daniel Schramek,&nbsp;G. Gregory Neely,&nbsp;Marina Pajic","doi":"10.1002/ggn2.202200014","DOIUrl":"10.1002/ggn2.202200014","url":null,"abstract":"<p>Cancer is the second leading cause of death globally, with therapeutic resistance being a major cause of treatment failure in the clinic. The dynamic signaling that occurs between tumor cells and the diverse cells of the surrounding tumor microenvironment actively promotes disease progression and therapeutic resistance. Improving the understanding of how tumors evolve following therapy and the molecular mechanisms underpinning de novo or acquired resistance is thus critical for the identification of new targets and for the subsequent development of more effective combination regimens. Simultaneously targeting multiple hallmark capabilities of cancer to circumvent adaptive or evasive resistance may lead to significantly improved treatment response in the clinic. Here, the latest applications of functional genomics tools, such as clustered regularly interspaced short palindromic repeats (CRISPR) editing, to characterize the dynamic cancer resistance mechanisms, from improving the understanding of resistance to classical chemotherapeutics, to deciphering unique mechanisms that regulate tumor responses to new targeted agents and immunotherapies, are discussed. Potential avenues of future research in combating therapeutic resistance, the contribution of tumor–stroma signaling in this setting, and how advanced functional genomics tools can help streamline the identification of key molecular determinants of drug response are explored.</p>","PeriodicalId":72071,"journal":{"name":"Advanced genetics (Hoboken, N.J.)","volume":"3 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9993475/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9455822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}