Acta Botanica Yunnanica最新文献_第9页

Linkage Maps of the Genus Hedychium (Zingiberaceae) Based on SRAP 基于SRAP的姜科姜花属连锁图谱

Acta Botanica Yunnanica Pub Date : 2010-03-30 DOI: 10.3724/SP.J.1143.2009.09036

Gao Lixia, L. Nian, Huang BangHai

引用次数: 2

Flower development and cultivation of Paphiopedilum armeniacum (Orchidaceae). 兰花科罂粟花的发育与栽培。

Acta Botanica Yunnanica Pub Date : 2010-03-30 DOI: 10.3724/SP.J.1143.2009.09048

Pi Qiuxia, Y. Ning, H. Hong, Liang Shuyun

引用次数: 3

AFLP Analysis of Phryma (Phrymaceae) Disjunct between Eastern Asia and Eastern North America: AFLP Analysis of Phryma (Phrymaceae) Disjunct between Eastern Asia and Eastern North America 东亚与北美东部瑞草科瑞草科的AFLP分析:东亚与北美东部瑞草科瑞草科的AFLP分析

Acta Botanica Yunnanica Pub Date : 2010-03-30 DOI: 10.3724/SP.J.1143.2009.09075

Z. Nie, J. Wen, Hang Sun

引用次数: 1

Physiological effect of heat stress on pea (Pisum sativum) hypocotyls. 热胁迫对豌豆下胚轴的生理影响。

Acta Botanica Yunnanica Pub Date : 2010-03-30 DOI: 10.3724/SP.J.1143.2009.09017

Tian Xuejun, Tao Hong-zheng, Luo Jing, Zhang Xudong, Tao FaQing

引用次数: 7

Prokaryotic Expression and Bioinformatics Analysis of Cytosolic Malate Dehydrogenase from Camellia sinensis(Theaceae) 山茶细胞质苹果酸脱氢酶的原核表达及生物信息学分析

Acta Botanica Yunnanica Pub Date : 2010-01-01 DOI: 10.3724/sp.j.1143.2010.00032

Chenfei Lu

{"title":"Prokaryotic Expression and Bioinformatics Analysis of Cytosolic Malate Dehydrogenase from Camellia sinensis(Theaceae)","authors":"Chenfei Lu","doi":"10.3724/sp.j.1143.2010.00032","DOIUrl":"https://doi.org/10.3724/sp.j.1143.2010.00032","url":null,"abstract":"The complete gene of cytosolic malate dehydrogenase (cMDH) from Camellia sinensis,called Cs-cMDH,was obtained by RT-PCR and rapid amplification of cDNA ends (GenBank accession number GQ845406). This gene was 1 235 bp in length,encoding a protein of 332 amino acids with the putative molecular weight of 35.5 kD. The E.coli Rosetta (DE3) harboring pGEX-MDH was induced by 0.5 mmol·L-1 IPTG at 32℃ for 3 hours,and a 61.5 kD glutathione Stransferase (GST)-fused MDH was obtained in soluble form. The results of NCBI-BLAST revealed that Cs-cMDH shared 88%-93% of amino acid sequence identity with other cMDH from different higher plants. According to the multiple sequence alignment based on the three-dimensional structure of protein,Cs-cMDH was predicted to be a dimer with thirteen β-sheet and thirteen α-helix of each subunit. Cs-cMDH contains typical fingerprint sequence (G12AAGQIG18) as all MDHs. The amino acid D43 in Cs-cMDH is conserved in all NAD-MDHs. Cs-cMDH also has some conserved sequence units homologous to other NAD-MDHs,such as NAD+ binding sites,catalytic motif and substrate binding sites. Moreover,Cs-cMDH contains six Cys which are highly conserved in all plant NAD-cMDHs. Therefore,Cs-cMDH was inferred to be NAD-dependent cMDH. The present study may provide the fundament for the further functional characterization of Cs-cMDH.","PeriodicalId":7106,"journal":{"name":"Acta Botanica Yunnanica","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78513250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Preliminary Study on DNA Barcoding of Mosses——A Case of Part of Genera of Meteoriaceae 苔藓植物DNA条形码的初步研究——以气象科部分属为例

Acta Botanica Yunnanica Pub Date : 2010-01-01 DOI: 10.3724/sp.j.1143.2010.09234

Z. Li

{"title":"The Preliminary Study on DNA Barcoding of Mosses——A Case of Part of Genera of Meteoriaceae","authors":"Z. Li","doi":"10.3724/sp.j.1143.2010.09234","DOIUrl":"https://doi.org/10.3724/sp.j.1143.2010.09234","url":null,"abstract":"We compared the performances of the candidate loci for moss DNA barcoding and the primers used in amplifying the loci. Primers for three coded loci (matK,rps4 and rbcL-a) and four non-coded loci (atpB-rbcL,atpF-H,psbK-I and trnH-psbA) of the chloroplast genome,one from the mitochondrial genome (nad5),and one from the nucleus genome (ITS2) were evaluated. Seventy-four samples representing 14 species belonging to five genera of Trachypodoaceae (or Meteoriaceae) were screened. All primers for matK and a pair of primers for trnH-psbA failed. Low successes were encountered with the primers for atpF-H and psbK-I. The primers for psbK-I produced several bands and the PCR products of atpF-H were difficult to sequence. The powers of the remaining six loci were compared using the variability,identification success and the resolutions. It was found that ITS2 is the most promising candidate for DNA barcoding for mosses. Among the chloroplast genes,atpB-rbcL exhibited the highest resolution. Although trnH-psbA is very variable,it is too short to be an ideal barcode alone. Combinations of chloroplast genes were also tried and Ps of both atpB-rbcL+trnH-psbA and rbcL-a++trnH-psbA were 64% using NJ method. More additions of loci did not increase the resolution. No barcoding gap exists for all these loci. Phylogenetic analyses were carried out prior to the DNA barcoding evaluation and some taxonomic problems do exist. This study exemplifies the necessity of correct species delimitation and the adoption of both plastid and nuclear loci in plant DNA barcoding.","PeriodicalId":7106,"journal":{"name":"Acta Botanica Yunnanica","volume":"105 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78548064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

New Invasive and New Distribution Species of Spermatophyte in Yunnan 云南种子植物新入侵及新分布种

Acta Botanica Yunnanica Pub Date : 2010-01-01 DOI: 10.3724/sp.j.1143.2010.

W. Huan

引用次数: 0

Phylogeny of Camellia sects. Longipedicellata,Chrysantha and Longissima(Theaceae) Based on Sequence Data of Four Chloroplast DNA Loci 山茶属植物的系统发育。基于四个叶绿体DNA位点序列数据的龙骨花、菊花和龙骨花(山茶科)

Acta Botanica Yunnanica Pub Date : 2010-01-01 DOI: 10.3724/sp.j.1143.2010.00001

Y. Jun

{"title":"Phylogeny of Camellia sects. Longipedicellata,Chrysantha and Longissima(Theaceae) Based on Sequence Data of Four Chloroplast DNA Loci","authors":"Y. Jun","doi":"10.3724/sp.j.1143.2010.00001","DOIUrl":"https://doi.org/10.3724/sp.j.1143.2010.00001","url":null,"abstract":"We focused on the systematic positions and relationships of three sections of Camellia subgen.Thea of Theaceae,i.e.,sect.Longipedicellata,sect.Chrysantha (golden camellias) and sect.Longissima by using four chloroplast DNA regions (rpl16,psbA-trnH,trnL-F rpl32-trnL). We sampled 28 species representing four subgenus,11 sections in Camellia and two outgroups. Combined analyses of chloroplast DNA sequence data sets are performed with the neighbor-joining,maximum-parsimony and Bayesian inference methods,and the gene trees are constructed. The topologies of gene trees revealed that:1) sect. Chrysantha is paraphyletic or polyphyletic,containing three parallel lineages and Camellia longipedicellata (type of sect.Longipedicellata) nested inside; 2) all four species from Vietnam,together with C. longipedicellata,forming a well-supported monophyletic clade,which implies the close relationship between sect.Longipedicellata and sect.Chrysantha; and 3) sect.Longissima is not monophyletic because C.hekouensis is the sister to the rest of Camellia species. The systematic position of C.longissima and the relationship between sect.Longissima and sect.Longipedicellata are unsolved.","PeriodicalId":7106,"journal":{"name":"Acta Botanica Yunnanica","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74686591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A New Phenylpropanoid Glucoside from Remusatia vivipara(Araceae) 天南星科活蕨中一个新的苯丙类糖苷

Acta Botanica Yunnanica Pub Date : 2010-01-01 DOI: 10.3724/sp.j.1143.2010.00087

Tang Gui

引用次数: 1

Cloning and analyses of a dual specific serine/thronine protein kinase gene with high conservative and constitutive expression in Oryza (OsSTK). 水稻(OsSTK)高保守性和组成性表达的丝氨酸/苏氨酸蛋白激酶双特异性基因的克隆与分析。

Acta Botanica Yunnanica Pub Date : 2009-10-25 DOI: 10.3724/SP.J.1143.2009.09096

Yang Mingzhi, Huang Xingqi, Zhang Hanbo, C. Shan-na, Yang Hongyu, Cheng Zaiquan

引用次数: 0