Acta cirurgica brasileira最新文献_第9页

Protective effect of hydroxysafflor yellow A on cyclosporin A-induced renal oxidative stress in vitro and in vivo. 羟基红花黄A对环孢素A诱导的肾脏氧化应激的保护作用。

IF 1.1 4区医学

Acta cirurgica brasileira Pub Date : 2022-06-15 eCollection Date: 2022-01-01 DOI: 10.1590/acb370305

Jiyuan Wang, Yu Chen

{"title":"Protective effect of hydroxysafflor yellow A on cyclosporin A-induced renal oxidative stress in vitro and in vivo.","authors":"Jiyuan Wang, Yu Chen","doi":"10.1590/acb370305","DOIUrl":"https://doi.org/10.1590/acb370305","url":null,"abstract":"Purpose: To explore the mechanism and investigate the protective effect of hydroxysafflor yellow A (HSYA) on renal oxidative stress, which cyclosporine A (CsA) induces.Methods: HK-2 cells were treated with CsA to get CsA-induced oxidative stress. The effects on oxidative stress and apoptosis of HK-2 cells were detected. The contents of SOD, MDA, GSH-Px, ROS, and CAT in serum were measured, and the expression of apoptosis-related proteins was detected by western blot. Then, established the renal oxidative stress injury rats to verify the efficacy of HSYA.Results: HSYA could reduce the ROS and MDA contents induced by CsA. Compared with the CsA group, the activities of SOD, CAT, and GSH-Px increased significantly when treated with HSYA. HSYA could inhibit CsA-induced apoptosis in HK-2 cells, and promote the protein of Bcl-2 and inhibit the expression of Bax. Animal experiments showed that HSYA could reduce CsA-induced renal cell injury by reducing glomerular cell vacuoles and inflammatory factors in tissues. It also decreased serum creatinine (Crea) and blood urea nitrogen, increased Crea clearance significantly.Conclusions: HSYA could significantly improve the antioxidant capacity of the kidney cells and inhibit cell apoptosis, thereby effectively ameliorating CsA-induced oxidative stress in vitro and in vivo.","PeriodicalId":6992,"journal":{"name":"Acta cirurgica brasileira","volume":" ","pages":"e370305"},"PeriodicalIF":1.1,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9211037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40178858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing ultrasonographic optic nerve sheath diameter in animal model with anesthesia regimens. 麻醉下动物模型视神经鞘直径的超声评估。

IF 1.1 4区医学

Acta cirurgica brasileira Pub Date : 2022-06-15 eCollection Date: 2022-01-01 DOI: 10.1590/acb370308

Maira de Robertis Azevedo, Marcelo de-Lima-Oliveira, Alessandro Rodrigo Belon, Sérgio Brasil, Manoel Jacobsen Teixeira, Wellingson Silva Paiva, Edson Bor-Seng-Shu

{"title":"Assessing ultrasonographic optic nerve sheath diameter in animal model with anesthesia regimens.","authors":"Maira de Robertis Azevedo, Marcelo de-Lima-Oliveira, Alessandro Rodrigo Belon, Sérgio Brasil, Manoel Jacobsen Teixeira, Wellingson Silva Paiva, Edson Bor-Seng-Shu","doi":"10.1590/acb370308","DOIUrl":"https://doi.org/10.1590/acb370308","url":null,"abstract":"Purpose: To determine the normal optical nerve sheath (ONS) diameter ultrasonography (ONSUS) and evaluate the possible effects of drugs on ONS diameter during anesthetic induction in healthy pigs.Methods: Healthy piglets were divided into three groups: a control group, that received xylazine and ketamine (X/K); other that received xylazine, ketamine and propofol (X/K/P); and a third group that received xylazine, ketamine, and thiopental (X/K/T). The sheath diameter was assessed by ultrasonography calculating the average of three measurements of each eye from the left and right sides.Results: 118 animals were anesthetized (49 X/K 33 X/K/P and 39 X/K/T). Mean ONS sizes on both sides in each group were 0.394 ± 0.048 (X/K), 0.407 ± 0.029 (X/K/P) and 0.378 ± 0.042 cm (X/K/T) (medians of 0.400, 0.405 and 0.389, respectively). The ONS diameter varied from 0.287-0.512 cm (mean of 0.302 ± 0.039 cm). For group X/K, the mean diameter was 0.394 ± 0.048 cm. Significant differences in ONS sizes between the groups P and T (X/K/P > X/K/T, p = 0.003) were found. No statistically significant differences were detected when other groups were compared (X/K = X/K/P, p = 0.302; X/K = X/K/T, p = 0.294).Conclusions: Sedation with thiopental lead to significative ONS diameter reduction in comparison with propofol. ONSUS may be useful to evaluate responses to thiopental administration.","PeriodicalId":6992,"journal":{"name":"Acta cirurgica brasileira","volume":" ","pages":"e370308"},"PeriodicalIF":1.1,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208240/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40178859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Gynura segetum induces hepatic sinusoidal obstruction syndrome in mice by impairing autophagy. 黄蚕通过损害自噬诱导小鼠肝窦梗阻综合征。

IF 1.1 4区医学

Acta cirurgica brasileira Pub Date : 2022-02-18 eCollection Date: 2022-01-01 DOI: 10.1590/ACB361104

Hui Zhang, Shu Jia, Lianyu Jin, Jianzuo Yao Mb, Zhihong Shen, Jingyi Wu, Xiaokun Yao, Danwei Chen, Congcong Zhang, Shufang Yu, Ningwei Zhu, Lexiao Jin, Xiaomin Yao

{"title":"Gynura segetum induces hepatic sinusoidal obstruction syndrome in mice by impairing autophagy.","authors":"Hui Zhang, Shu Jia, Lianyu Jin, Jianzuo Yao Mb, Zhihong Shen, Jingyi Wu, Xiaokun Yao, Danwei Chen, Congcong Zhang, Shufang Yu, Ningwei Zhu, Lexiao Jin, Xiaomin Yao","doi":"10.1590/ACB361104","DOIUrl":"https://doi.org/10.1590/ACB361104","url":null,"abstract":"Purpose: To investigate the underlying mechanism of hepatic sinusoidal obstruction syndrome (HSOS) induced by Gynura segetum by measuring autophagy in mouse models.Methods: The model group was administered G. segetum (30 g/kg/d) by gavage, while the normal control group was administered an equal volume of saline daily for five weeks. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), hepatic histopathological examinations, and Masson staining were performed to evaluate liver injury. Liver intercellular adhesion molecule-1 (ICAM-1) and P-selectin were evaluated by immunohistochemistry. Hepatocellular apoptosis was assessed using the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay. Protein expression levels of autophagy markers were measured using Western blot analysis.Results: Gynura segetum was found to significantly induce liver injury compared with control mice, as evidenced by the increase of serum transaminases, a decrease in triglyceride levels, and histopathological changes in mice. Gynura segetum remarkably induced hepatocellular apoptosis and upregulated the expressions of ICAM-1 and P-selectin and also downregulated the protein expression levels of LC3, Atg12 and cytoplasmic polyadenylation element binding protein.Conclusions: Our results suggested that G. segetum induced liver injury with HSOS, and it was partly due to its ability to impair the autophagy pathway.","PeriodicalId":6992,"journal":{"name":"Acta cirurgica brasileira","volume":"36 11","pages":"e361104"},"PeriodicalIF":1.1,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8860401/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39945122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Paeoniflorin mitigates PBC-induced liver fibrosis by repressing NLRP3 formation. 芍药苷通过抑制NLRP3的形成减轻pbc诱导的肝纤维化。

IF 1.1 4区医学

Acta cirurgica brasileira Pub Date : 2022-02-18 eCollection Date: 2022-01-01 DOI: 10.1590/ACB361106

Yizhe Zhang, Shujie Zhang, Xin Luo, Han Zhao, Xiaoxing Xiang

{"title":"Paeoniflorin mitigates PBC-induced liver fibrosis by repressing NLRP3 formation.","authors":"Yizhe Zhang, Shujie Zhang, Xin Luo, Han Zhao, Xiaoxing Xiang","doi":"10.1590/ACB361106","DOIUrl":"https://doi.org/10.1590/ACB361106","url":null,"abstract":"Purpose: To delve into the influence of paeoniflorin (PA) on abating primary biliary cholangitis (PBC)-induced liver fibrosis and its causative role.Methods: Our team allocated the mice to control group, PA group, PBC group and PBC+PA group. We recorded the weight change of mice in each group. We used Masson staining for determining liver fibrosis, immunofluorescence staining for measuring tumor necrosis factor-α (TNF-α) expression, quantitative real-time polymerase chain reaction (qRT-PCR) for assaying related gene expression, as well as Western blot for testing related protein expression.Results: The weight of PBC model mice declined. Twenty-four weeks after modeling, the positive rate of anti-mitochondrial antibody-M2 (AMA-M2) in PBC mice reached 100%. Alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), hydroxyproline (HYP), laminin (LN), procollagen type III (PC III), and malondialdehyde (MDA) contents saliently waxed (p<0.01). Meanwhile, superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) activity patently waned (p<0.01). Liver fibrosis levels were flagrantly higher (p<0.01), and TNF-α, NOD-like receptor protein 3 (NLRP3), caspase-1, interleukin-18 (IL-18), and interleukin-1β (IL-1β) protein or gene expression were manifestly up-regulated (p<0.01). PA could restore the weight of PBC mice, strikingly restrain the positive expression of AMA-M2, and down-regulate serum ALP, ALT, AST, HYP, LN, PC III, MDA in PBC mice (p<0.01). PA could also significantly up-regulate SOD and GSH-px levels (p<0.01), down-regulate IL-1β, IL-18, caspase-1, NLRP3, and TNF-α protein or gene expression in PBC mice (p<0.01) and inhibit liver fibrosis levels (p<0.01).Conclusions: PA can reduce PBC-induced liver fibrosis in mice and may function by curbing the formation of NLRP3.","PeriodicalId":6992,"journal":{"name":"Acta cirurgica brasileira","volume":"36 11","pages":"e361106"},"PeriodicalIF":1.1,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8860402/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39945123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Pigeon as a model to study peripheral projections from the horizontal semicircular canal vestibular apparatus to a brainstem target immunoreactive for AMPA. 以鸽子为模型，研究从水平半规管前庭器官到脑干靶免疫反应AMPA的外周投射。

IF 1.1 4区医学

Acta cirurgica brasileira Pub Date : 2022-01-05 eCollection Date: 2022-01-01 DOI: 10.1590/ACB361206

João Roberto Rocha, Maria de Fátima Passetto, Julianne Dos Santos Maldonado-Menetti, Ana Lúcia Beirão Cabral, Claudio Antônio Barbosa de Toledo, Marcia Koike

{"title":"Pigeon as a model to study peripheral projections from the horizontal semicircular canal vestibular apparatus to a brainstem target immunoreactive for AMPA.","authors":"João Roberto Rocha, Maria de Fátima Passetto, Julianne Dos Santos Maldonado-Menetti, Ana Lúcia Beirão Cabral, Claudio Antônio Barbosa de Toledo, Marcia Koike","doi":"10.1590/ACB361206","DOIUrl":"https://doi.org/10.1590/ACB361206","url":null,"abstract":"Purpose: To evaluate whether the pigeon (Columba livia) is a good model for evaluating the vestibular system involved with postural maintenance during movement.Methods: This study maps the brainstem targets of the horizontal ampullary inputs from the vestibular periphery of the pigeon. We used biotin dextran amine (BDA) injection in horizontal semicircular canal (HSCC), immunohistochemistry for GluR2/3 and GluR4 AMPA and computerized histomorphology reconstruction.Results: Our results show the same distribution pattern with ipsilateral projections to vestibular nuclear complex (VNC) from the HSCC, with the majority of labeled fibers being, long, thin, with few varicosities and many ramifications. Horizontal semicircular canal projections achieve neurons belonging to all nuclei of the VNC with exception of dorsal portion of lateral vestibular nucleus and this area express GluR2/3 and GluR4 AMPA receptors reinforcing the idea of glutamate participation in these connections.Conclusions: Pigeon is an appropriated experimental model to study of projections of HSCC and reinforcing the information that the vestibular system has strong relation with the fast responses necessary for postural control. Moreover, its phylogenetic organization apparently conservation, also seems to be a fundamental characteristic for vertebrates.","PeriodicalId":6992,"journal":{"name":"Acta cirurgica brasileira","volume":"36 12","pages":"e361206"},"PeriodicalIF":1.1,"publicationDate":"2022-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8734960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39812977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Skin graft associated with platelet-rich plasma in correcting extensive injuries resulting from the resection of skin cancer chemically induced in rats. 富血小板血浆皮肤移植对大鼠化学诱导的皮肤癌切除术后大面积损伤的修复作用。

IF 1.1 4区医学

Acta cirurgica brasileira Pub Date : 2022-01-05 eCollection Date: 2022-01-01 DOI: 10.1590/ACB361203

Josiane Morais Pazzini, Stella Habib Moreira, Pedro Cassino, Marjury Maronezi, Michelle Zangirolami, Jorge Luis Alvaréz Gomés, Paulo Henrique Bertolo, Michelle do Carmo Pereira Rocha, Sonia Prince Fiebi, Caroline Kajiura, Filippo Janoni Bernardes, Caio Bustamante, Andrigo Barboza De Nardi

{"title":"Skin graft associated with platelet-rich plasma in correcting extensive injuries resulting from the resection of skin cancer chemically induced in rats.","authors":"Josiane Morais Pazzini, Stella Habib Moreira, Pedro Cassino, Marjury Maronezi, Michelle Zangirolami, Jorge Luis Alvaréz Gomés, Paulo Henrique Bertolo, Michelle do Carmo Pereira Rocha, Sonia Prince Fiebi, Caroline Kajiura, Filippo Janoni Bernardes, Caio Bustamante, Andrigo Barboza De Nardi","doi":"10.1590/ACB361203","DOIUrl":"https://doi.org/10.1590/ACB361203","url":null,"abstract":"Purpose: To evaluate whether using platelet-rich plasma (PRP) in the graft recipient bed after the resection of a neoplasia can influence its recurrence because this product stimulates angiogenesis, mitogenesis and chemotaxis.Methods: A study with 30 rats Wistar (Rattus norvegicus albinus), which were separated into group A (induction of carcinogenesis, PRP in the postoperative period) and group B (induction of carcinogenesis, absence of PRP in the postoperative period), with 15 animals in each. Carcinogenesis was induced on the skin of the animals' chest by the topical application of 0.5% dimethylbenzantracene (DMBA) diluted in acetone. After surgical resection of the induced neoplasia, PRP was used to stimulate angiogenesis before surgical wound synthesis. Data on the control and experimental groups and macroscopic and microscopic variables were evaluated using analysis of variance and the Tukey's test (5%).Results: It was possible to determine that the use of PRP is good in reconstructive surgeries, but it is contraindicated in patients during tumor resection, as it can cause changes in the surgical bed, in addition to stimulating recurrences and metastases.Conclusions: PRP may interact with tumour cells that were in the recipient site of the surgical wound during the resection of a neoplasia, and a local recurrence process can be triggered by applying this product.","PeriodicalId":6992,"journal":{"name":"Acta cirurgica brasileira","volume":"36 12","pages":"e361203"},"PeriodicalIF":1.1,"publicationDate":"2022-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8734959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39926989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological compatibility of oxidized cellulose vs. porcine gelatin to control bleeding in liver lesions in rats. 氧化纤维素与猪明胶控制大鼠肝损伤出血的生物相容性。

IF 1.1 4区医学

Acta cirurgica brasileira Pub Date : 2022-01-05 eCollection Date: 2022-01-01 DOI: 10.1590/ACB361101

Maria de Lourdes Pessole Biondo-Simões, Jaqueline Alves Zwierzikowski, Juliane Castro Duarte Antoria, Sérgio Ossamu Ioshii, Rogério Ribeiro Robes

{"title":"Biological compatibility of oxidized cellulose vs. porcine gelatin to control bleeding in liver lesions in rats.","authors":"Maria de Lourdes Pessole Biondo-Simões, Jaqueline Alves Zwierzikowski, Juliane Castro Duarte Antoria, Sérgio Ossamu Ioshii, Rogério Ribeiro Robes","doi":"10.1590/ACB361101","DOIUrl":"https://doi.org/10.1590/ACB361101","url":null,"abstract":"Purpose: To compare biological compatibility, hemostasis, and adhesion formation between oxidized regenerated cellulose and lyophilized hydrolyzed porcine collagen in liver trauma.Methods: Forty male Wistar rats constituted two groups: group A (oxidized cellulose) and group B (lyophilized hydrolyzed collagen). Standardized liver trauma was made, and the hemostatic agent was applied. Animals in subgroups A7 and B7 were submitted to euthanasia and relaparotomy after seven days, and in subgroups A14 and B14 after 14 days. Macroscopic and microscopic results were evaluated.Results: There was no fluid in the cavity in any of the animals, and adhesions were present in all of them. In the analysis after seven days, the adhesions were grades 3 or 4 and consisted of omentum, small intestine, and abdominal wall (p<0.05). In both groups, the mesh was surrounded by a capsule, which was not observed after 14 days. In the evaluation after 14 days, adhesions were grades 2 or 3 (p>0.05). The microscopic examination showed subacute and chronic reactions, in both groups and in both timepoints, with similar frequency. The intensity of fibrosis always presented positive scores. Microabscesses and xanthomatous macrophages were observed in both groups.Conclusions: There was no superiority of one agent over the other.","PeriodicalId":6992,"journal":{"name":"Acta cirurgica brasileira","volume":"36 11","pages":"e361101"},"PeriodicalIF":1.1,"publicationDate":"2022-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8734958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39902709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of one-time high load exercise on skeletal muscle injury in rats of different genders: oxidative stress and mitochondrial responses. 一次性高负荷运动对不同性别大鼠骨骼肌损伤的影响:氧化应激和线粒体反应。

IF 1.1 4区医学

Acta cirurgica brasileira Pub Date : 2022-01-01 DOI: 10.1590/acb370805

Yuan Wang, Mengmeng Chen, Yan Gao, Kang He, Zhaoyun Yang, Yuewei Li, Shuang Zhang, Lijing Zhao

{"title":"Effect of one-time high load exercise on skeletal muscle injury in rats of different genders: oxidative stress and mitochondrial responses.","authors":"Yuan Wang, Mengmeng Chen, Yan Gao, Kang He, Zhaoyun Yang, Yuewei Li, Shuang Zhang, Lijing Zhao","doi":"10.1590/acb370805","DOIUrl":"https://doi.org/10.1590/acb370805","url":null,"abstract":"Purpose: To evaluate the impact of one-time high load exercise on skeletal muscle injury and analysis its mechanism in different genders.Methods: Twenty-four male and 24 female rats were divided randomly into four groups respectively: control, 0 h, 6 h, and 24 h after exercise. The activities of creatine kinase (CK), lactate dehydrogenase (LDH), and myohemoglobin (MYO) in serum, the expression level of oxidative stress markers, mitochondrial respiratory chain complex enzyme, and the apoptosis related protein in quadriceps were detected.Results: The results showed that the activities of CK, LDH and MYO in serum increased immediately after exercise and restored faster in female rats. More obvious structural disorder and apoptosis in male rats were showed. Malondialdehyde (MDA) and superoxide dismutase (SOD) were increased while catalase (CAT) and glutathione (GSH) were decreased in male rats. SOD, CAT and GSH were increased in female rats. Mitochondrial complex enzyme activity was decreased in males and increased in females.Conclusions: The skeletal muscle injury in both genders of rat could be induced by one-time high load exercise due to the mitochondrial respiratory enzyme dysfunction and oxidative stress, which was relatively mild and recovered quicker in female rats.","PeriodicalId":6992,"journal":{"name":"Acta cirurgica brasileira","volume":"37 8","pages":"e370805"},"PeriodicalIF":1.1,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9746543/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10399174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Treatment of experimentally induced partial-thickness burns in rats with different silver-impregnated dressings. 不同浸银敷料对实验性大鼠局部烧伤的治疗作用。

IF 1.1 4区医学

Acta cirurgica brasileira Pub Date : 2022-01-01 DOI: 10.1590/acb370801

Carolyna de Sousa Carvalho, Milton Junio Cândido Bernardes, Randys Caldeira Gonçalves, Marielle Sousa Vilela, Marcus Vinicius Meneses da Silva, Vinicius da Silva Oliveira, Marcelo Ribeiro da Rocha, Marina Clare Vinaud, Hélio Galdino Junio, Ruy de Souza Lino Junior

{"title":"Treatment of experimentally induced partial-thickness burns in rats with different silver-impregnated dressings.","authors":"Carolyna de Sousa Carvalho, Milton Junio Cândido Bernardes, Randys Caldeira Gonçalves, Marielle Sousa Vilela, Marcus Vinicius Meneses da Silva, Vinicius da Silva Oliveira, Marcelo Ribeiro da Rocha, Marina Clare Vinaud, Hélio Galdino Junio, Ruy de Souza Lino Junior","doi":"10.1590/acb370801","DOIUrl":"https://doi.org/10.1590/acb370801","url":null,"abstract":"Purpose: To evaluate the morphometric, macroscopic and microscopic aspects of experimentally induced partial-thickness burns in rats treated with different silver-based dressings.Methods: Wistar rats were used, divided into six treatments: saline (NaCl 0.9%); silver sulfadiazine 1%; Silvercel; Mepilex Ag; Aquacel Ag and Acticoat. The animals were monitored daily and euthanized at 7, 14 and 30 days after injury induction (DAI).Results: At 7 DAI, necrosis/crust was greater in control, silver sulfadiazine and Mepilex Ag treatments, granulation tissue was induced by Aquacel Ag, polymorphonuclear infiltrate (PMN) infiltration was intensified by Mepilex Ag; mononuclear infiltrate (MN) infiltration and angiogenesis were increased by Silvercel. At 14 DAI, hemorrhage was decreased by Silvercel and Mepilex Ag, PMN infiltration increased by Acticoat. At 30 DAI, angiogenesis was greater in the Acticoat treatment and fibroblasts were increased by Acticoat and Mepilex Ag. Collagen was induced at 14 DAI by silver sulfadiazine and Aquacel Ag and, at 30 DAI, by silver sulfadiazine and Silvercel treatments.Conclusions: Silvercel and Acticoat presented better results than the other products. However, all the dressings were better than the control at some point during the process, and may contribute to the healing of partial thickness burns. Silvercel and Aquacel Ag treatments induced better cosmetic outcomes regarding wound closure and scarring.","PeriodicalId":6992,"journal":{"name":"Acta cirurgica brasileira","volume":"37 8","pages":"e370801"},"PeriodicalIF":1.1,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9708113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10678212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Licorice zinc suppresses melanogenesis via inhibiting the activation of P38MAPK and JNK signaling pathway in C57BL/6J mice skin. 甘草锌通过抑制C57BL/6J小鼠皮肤P38MAPK和JNK信号通路的激活来抑制黑色素生成。

IF 1.1 4区医学

Acta cirurgica brasileira Pub Date : 2022-01-01 DOI: 10.1590/acb371002

Jing-Yan Wang, Xing-Yu Xie, Ying Deng, Hong-Qiu Yang, Xiao-Shuang Du, Ping Liu, Yu Du

{"title":"Licorice zinc suppresses melanogenesis via inhibiting the activation of P38MAPK and JNK signaling pathway in C57BL/6J mice skin.","authors":"Jing-Yan Wang, Xing-Yu Xie, Ying Deng, Hong-Qiu Yang, Xiao-Shuang Du, Ping Liu, Yu Du","doi":"10.1590/acb371002","DOIUrl":"https://doi.org/10.1590/acb371002","url":null,"abstract":"Purpose: The active melanocytes in the skin were affected by hormones and ultraviolet (UV) irradiation. Licorice zinc has a whitening effect, which may have a prominent potential in the treatment of pigmented skin disease.Methods: Modeling chloasma C57BL/6J mice by daily progesterone injection (15 mg/kg) and ultraviolet B (UVB) irradiation (λ = 312 nm, 2 h/day) for 30 days. Then, mice were given 0.65, 1.3, and 2.6 (g/kg) of licorice zinc and tranexamic acid 250 mg daily by oral administration for 14 days, respectively. Hematoxylin and eosin and Fontana-Masson staining, and Western blotting (WB) were performed to test the inhibitory of melanogenesis and activation of c-Jun-N-terminal (JNK)/p38 mitogen-activated protein kinases (MAPK) for licorice zinc. Melanogenesis was induced by α-melanocyte-stimulating hormone in vitro. Cell counting kit-8, melanin content determination, and WB were performed to verify the inhibitory effect of licorice zinc on melanogenesis.Results: The present study showed that licorice zinc decreased melanin formation, cutaneous tissue injury, and the phosphorylation of JNK and P38MAPK, which was caused by UVB irradiation in vivo. In vitro, licorice zinc showed opposite effects from JNK/p38 activator. Meanwhile, tyrosinase-related protein-1, tyrosinase, and microphthalmia-associated transcription factor were decreased too.Conclusions: Licorice zinc induced a decrease in melanin synthesis by inhibiting the JNK and the P38MAPK signaling pathway, suggesting licorice zinc is a potential agent of anti-chloasma.","PeriodicalId":6992,"journal":{"name":"Acta cirurgica brasileira","volume":"37 10","pages":"e371002"},"PeriodicalIF":1.1,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9762428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9765801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2