Acta Biomedica Scientifica (East Siberian Biomedical Journal)最新文献

{"title":"Successful Experience of Fecal Transplantation in a Patient with Severe Pseudomembranous Colitis","authors":"L. Shedoeva, E. Chashkova, A. S. Rubtsov","doi":"10.29413/abs.2020-5.6.32","DOIUrl":"https://doi.org/10.29413/abs.2020-5.6.32","url":null,"abstract":"Introduction. At the present time there is increase the number of patients diagnosed with pseudomembranous colitis due to Clostridiodes difficile, respectively it significantly increases the frequency of hospitalizations, the length of hos - pital stay and the cost of treatment. Approved drug methods for correcting a pathological condition are not always able to lead to recovery after the first use, repeated and prolonged courses of therapy are often required, especially with recurrent Clostridiodes difficile. An alternative way of treatment is fecal microbiota transplantation from a donor. Nowadays, fecal microbiota transplantation is included in national clinical guidelines in several countries in Europe, the USA and Australia. In Russia this method has not been registered, however, there are some publications about its successful application in gastrointestinal diseases, oncohematology, and some autoimmune diseases. Aim. To show the result of successful treatment of severe pseudomembranous colitis after a single fecal microbiota transplantation. Material and methods. Outpatient treatment of an acute respiratory infection with a broad-spectrum antibacterial drug caused the development of severe pseudomembranous colitis in a young patient. Standard courses of metronidazole and vancomycin were unsuccessful. Transplantation of fecal microbiota from a donor was performed via ileocolonoscopy. Results. A single fecal microbiota transplantation contributed to the patient’s complete recovery, short term rehabilita tion and the absence of recurrence of Clostridiodes difficile within two years. Conclusions. Evidence-based medicine has shown the high efficiency of fecal microbiota transplantation. In Russia a legislative basis is needed for including fecal microbiota transplantation in clinical guidelines for the treatment of severe Clostridiodes difficile resistant to standard therapy.","PeriodicalId":6986,"journal":{"name":"Acta Biomedica Scientifica (East Siberian Biomedical Journal)","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77394802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Premature Ovarian Insufficiency in a Popular Sample of Women in the Pribaykalsky Region: Prevalence and Age Features","authors":"M. Salimova, Ya. G. Nadelyaeva","doi":"10.29413/abs.2020-5.6.4","DOIUrl":"https://doi.org/10.29413/abs.2020-5.6.4","url":null,"abstract":"Premature ovarian insufficiency (POI) is a life-changing diagnosis, with profound physical and psychological consequences. Objective. To determine the prevalence, clinical features and hormone characteristics of women with premature ovarian failure of different age group. Materials and methods. During the study we examined 1200 women, aged 18–45 years. Of all examined women, 17 patients with clinical and hormonal signs of POI under the age of 40 were identified. All participants signed an informed consent and underwent general clinical, instrumental and hormonal examination. Statistical analysis was performed using the Statistica 10.0. Results. The prevalence of POI among women of reproductive age was 1.4 % (1200/17). The most common clinical signs: a feeling of «heat», increased sweating and dryness in the vagina. Hot flashes were more often during the manifestation of the disease (50 %). An increase in FSH concentration was noted (26.14 ± 33.4 mIU/mL). The concentrations of thyroid hormone and prolactin were slightly higher in women aged 30–35 years, compared with older patients in the group of women at the onset of the disease in women from 35 to 40. Prolactin levels in women under 35 years old were 431.86 ± 357.82 and 387.13 ± 207.54 mU/L in the patients aged 35–40 years, accordingly (p > 0.05) Conclusion. It was established that the frequency of POF in Eastern Siberia does not differ from the frequency of occurrence of this disease in the world. The progression of clinical manifestations of hypoestrogenism occurs gradually. * Статья опубликована по материалам доклада на IV Всероссийской научно-практической конференции молодых учёных с международным участием «Фундаментальные и прикладные аспекты в медицине и биологии» (Иркутск, 16 октября 2020 года). ActA BiomedicA ScientificA, 2020, Vol. 5, N 6 east Siberian Biomedical Journal 38 Obstetrics and gynaecology Hot flashes were the earliest menopausal complaints. In women aged 30–35 years, POI is accompanied by a relatively higher activity of prolactinergic and thyrotropic pituitary functions.","PeriodicalId":6986,"journal":{"name":"Acta Biomedica Scientifica (East Siberian Biomedical Journal)","volume":"52 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91434777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activity of Lipoperoxidation – Antioxidant Protection Reactions in Patients with HIV Infection (Review)","authors":"O. Nikitina","doi":"10.29413/abs.2020-5.6.14","DOIUrl":"https://doi.org/10.29413/abs.2020-5.6.14","url":null,"abstract":"","PeriodicalId":6986,"journal":{"name":"Acta Biomedica Scientifica (East Siberian Biomedical Journal)","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88867254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Interleukin-10 Gene Polymorphisms with Ulcerative Colitis","authors":"I. V. Zhilin, E. Chashkova, A. Zhilina, A. Markovskiy, A. Kim","doi":"10.29413/abs.2020-5.6.7","DOIUrl":"https://doi.org/10.29413/abs.2020-5.6.7","url":null,"abstract":"This literature review is devoted to the study of the role of polymorphic variants of the anti-inflammatory interleukin-10 gene (Interleukin-10, IL10) in the etiology and pathogenesis of ulcerative colitis in patients of various ethnic groups. Aim. To generalize the results obtained from electronic databases on the study of the characteristics of the course of ulcerative colitis in patients of different ethnic groups, taking into account the carriage of IL10 gene polymorphisms. Materials and methods. Twenty-five studies were selected, including four meta-analyzes devoted to the study of associative relationships of single nucleotide polymorphisms (SNPs) of the IL10 gene isolated from blood samples at positions -592AA/CA, -819CT, -1082АА/GA, and rs3024505 in the development and course of ulcerative colitis. Results. A number of researchers from Europe, Saudi Arabia report an increased incidence of ulcerative colitis among carriers of the IL10-1082AA SNP and a low incidence among carriers of -592AA. Positive associations of IL10-819CT with the prevalence of ulcerative colitis are presented in single reports from Europe, the Middle East and in a number of studies from Asian countries. However, the identified associations, even within the same population, are often contradictory. Perhaps the discrepancies are due to the ethnic heterogeneity of the groups in the considered cohorts, and therefore it is necessary to continue epidemiological studies with a large sample size in specific geographic areas.","PeriodicalId":6986,"journal":{"name":"Acta Biomedica Scientifica (East Siberian Biomedical Journal)","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86032676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined Transosseous Osteosynthesis of Fracture of Humerus Diaphysis in Case of Reparative Process Disorder (Clinical Observation)","authors":"N. Tishkov","doi":"10.29413/ABS.2020-5.6.26","DOIUrl":"https://doi.org/10.29413/ABS.2020-5.6.26","url":null,"abstract":"The treatment of the consequences of diaphyseal fractures of the humerus, despite the emergence of modern effective methods of internal osteosynthesis, has not lost its relevance. Transosseous osteosynthesis remains the method of choice in the treatment of pseudoarthrosis and delayed consolidating fractures of the humerus. The experience we have accumulated in the use of external fixation devices in the treatment of fractures, based on the use of the method of unified designation of transosseous osteosynthesis, made it possible to systematize and describe the surgical technique of combined transosseous osteosynthesis in the treatment of fractures of the humerus diaphysis with impaired reparative processes in combination with longitudinal corticotomy of the  pseudarthrosis zone, and evaluate its effectiveness. The article presents a case of successful surgical treatment of a patient with a pseudarthrosis of the humerus diaphysis by the method of combined transosseous osteosynthesis in combination with longitudinal corticotomy of the pseudarthrosis zone. A description of the technique of transosseous osteosynthesis is given. The presented clinical case shows that the use of combined transosseous osteosynthesis, taking into account the method of unified designation of transosseous osteosynthesis, makes it  possible not only to determine the optimal arrangement of the apparatus and the positions of the transosseous elements, but also to use this method to determine the optimal area for performing longitudinal corticotomy.","PeriodicalId":6986,"journal":{"name":"Acta Biomedica Scientifica (East Siberian Biomedical Journal)","volume":"121 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74753795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multispiral Computed Tomography in the Assessment of the Timing of Acute Traumatic Intracranial Hematoma","authors":"A. V. Semenov, V. V. Krylov, V. Sorokovikov, O. P. Galeeva","doi":"10.29413/ABS.2020-5.6.17","DOIUrl":"https://doi.org/10.29413/ABS.2020-5.6.17","url":null,"abstract":"Background . Exact notion about the timing of acute traumatic intracranial hematomas (ATIH) generation is very important to gain a better understanding of the primary and secondary traumatic brain injury (TBI) pathophysiology. The variety of TBI and individual anatomical features of the victims determine the complexity of the issue, as well as the relevance of studies that bring it closer to its solution. Aim of the study was to assess the possibility of using multispiral computed tomography (MSCT) to determine the average timing of the formation of epidural and subdural ATIH. Materials and methods . It is a retrospective study of 84 MSCT investigations of 55 patients with ATIH (epidural – 15, subdural – 40) in Irkutsk City Clinical Hospital N 3 (2018–2019). The ATIH volume calculated by the MSCT. The cor relation of ATIH volumes with the period of trauma was studied, as well as a comparison  of average volumes of ATIH among patients distributed in groups in line with the hour of primary MSCT after injury. The dynamics of ATIH of small volumes (up to 40 ml) were assessed in 21 patients left under observation. Results . The volume of epidural ATIH and the time of their detection had no a direct correlation in the period 1 to 4 hours after the injury (p = 0.05). There was no reliable difference in their average volumes per hour of diagnosis, amounting to a total of 54.1 ± 19.7 ml. A direct correlation between the volume of subdural ATIH and the duration of trauma was identified during periods of 1–2 hours (p = 0.02) and 1–3 hours (p = 0.002; t = 3.77) after injury; a true difference in their average volume of 1 and 2 hours after the injury (52 ± 20.2 ml and 106.4 ± 14 ml, respectively). Increase in small ATIH found in 14.3 % of patients left under observation. Conclusions . The MSCT allows us to estimate the average timing of the formation of the main volume of ATIH when hospitalized victims with TBI. The average rate of accumulation of ATIH is about 50 ml per hour, and the direct correlation of their volume and time of diagnosis is reliable the first 2–3 hours after the injury  for subdural ATIH only.","PeriodicalId":6986,"journal":{"name":"Acta Biomedica Scientifica (East Siberian Biomedical Journal)","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79088815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Infectious Diseases Patterns in the Combined Use of Bacteriological Diagnostics and MALDI Biotyper","authors":"N. Voropaeva, N. Belkova, U. Nemchenko, E. Grigorova, E. Kungurtseva, O. A. Noskova, N. Chemezova, E. Savilov","doi":"10.29413/ABS.2020-5.6.10","DOIUrl":"https://doi.org/10.29413/ABS.2020-5.6.10","url":null,"abstract":"In multidisciplinary hospitals, there are conditions conducive to the emergence of healthcare-associated infections: high concentration of people with reduced immunity in a limited area, the presence of a significant number of sources of contagion (patients and carriers), a change in the biocenosis of the mucous membranes and skin of patients and medical personnel under the influence of widespread use of antibiotics and cytostatics. The aim of the research was in the intercomparison of the standardized bacteriological algorithms and the MALDI Biotyper system in the microbiological diagnosis of pathogens as illustrated by the healthcare-associated diseases. Materials and methods . Seventy-eight patients of a multidisciplinary hospital of a regional level (Irkutsk) in 2018–2019 were examined. The age of patients ranged from 1 to 15 years. The material for the study was blood, sputum, swabs from the tracheobronchial tree, throat, nose, wound, abdominal fluid, cerebrospinal fluid, and swabs from environmental objects. Identification of the isolated cultures (78 bacterial strains) was  carried out using generally accepted bacteriological methods, as well as using the MALDI Biotyper system. Results and discussions. In the structure of healthcare-associated infections, Pseudomonas aeruginosa occupied a leading position. Not all isolates of microorganisms were identified by standardized bacteriological methods. The identification of strains with characteristic manifestations of physiological and biochemical characteristics was more reliable. Identification difficulties arose in the presence of atypical properties of microorganisms when the use of MALDI Biotyper would be crucial. Conclusion . It is necessary to apply an integrated approach to conduct reliable diagnostics of pathogens. It includes standardized bacteriological methods and methods for identifying microorganisms using mass spectrometry in the subsequent stages.","PeriodicalId":6986,"journal":{"name":"Acta Biomedica Scientifica (East Siberian Biomedical Journal)","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90573914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Psychopathological Aspects of Somatization in the Works of the Department of Psychiatry of Irkutsk State Medical Institute in the First Half of the 20th Century – from the Origins of the Psychosomatic Medicine to the Present","authors":"V. Sobennikov, E. V. Vinokurov, V. V. Sobennikova","doi":"10.29413/abs.2020-5.4.11","DOIUrl":"https://doi.org/10.29413/abs.2020-5.4.11","url":null,"abstract":"The article discusses modern problems of dimensional diagnosis of somatoform disorders including the vagueness of the criteria and the lack of reliance on pathogenetic differences in the selection of separate classification categories. An ongoing discussion on the necessity of revision of the boundaries and of “redistribution” of some somatoform disorders into different categories in accordance with their psychopathological relationship is associated with these problems. As a promising change in the diagnostic criteria of the DSM-V classification, the emphasis is shifted to psychological and behavioral characteristics, which is assessed as an actualization of the classical descriptive diagnostics methodology. As a confirmation of the significance of this, an analysis of the psychopathological study of Irkutsk psychiatrists of the first half of the 20th century, devoted to the delusion of obsession, is given. In 1935, the head of the Department of Psychiatry at the Irkutsk Medical Institute V.S. Deryabin and his student I.S. Sumbaev published a scientific work “Delirium of obsession and somatic sensations”. The authors substantiate the intimate relationship of traditionally understood sensual hysterical and ideatorial hypochondriacal mechanisms in the formation of an integral clinical picture of somatoform disorders. The use of descriptive diagnostics many decades before the introduction of ICD-10 proves the pathogenetic unity of hysterical (sensory) and hypochondriacal (ideatorial) formations within the psychosomatic register of disorders and the legitimacy of combining phenomenologically different states into a circle of somatoform disorders.","PeriodicalId":6986,"journal":{"name":"Acta Biomedica Scientifica (East Siberian Biomedical Journal)","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78040520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Designing a Nutrient Medium for the Accumulation of Microbial Mass of Listeria","authors":"N. M. Khaptanova, S. V. Lukyanova, V. I. Kuznetsov, N. G. Gefan, N. M. Аndreevskaya, Z. A. Konovalova, А. S. Ostyak, V. S. Kosilko","doi":"10.29413/abs.2020-5.4.8","DOIUrl":"https://doi.org/10.29413/abs.2020-5.4.8","url":null,"abstract":"Background. To obtain reliable results of laboratory studies on the identification of Listeria, the presence of certified diagnostic agglutinating Listeria sera is required. An important step in the manufacturing process of such medical devices for in vitro diagnostics requires effective nutrient media for the accumulation of listeriosis microbe. Aim of the research. To develop an effective nutrient medium for the accumulation of bacterial mass of Listeria. Materials and methods. The object of the study was an experimental culture medium for Listeria cultivation. As a control, we used nutrient agar for the cultivation of microorganisms (fish meal hydrolysate, FMH-agar) and meat-peptone agar with 1 % glucose (MPA with 1 % glucose). The specific activity of nutrient media during cultivation of the test strain Listeria monocytogenes 766 was evaluated using a complex of microbiological methods. Results. The optimal base of the nutrient medium for Listeria cultivation has been selected: pancreatic hydrolysate of river magpie fish (Rutilus rutilus lacustris) and hydrolysate of meat water production waste. The qualitative and quantitative composition of the nutrient medium has been developed, its physical, chemical and biological properties have been studied. It was found that after 24 hours of incubation at 37 °C, the nutrient medium provided the growth of typical Listeria colonies. The germination rate was 85 %, which is higher compared to the growth of the culture on MPA with 1 % glucose and GRM agar by an average of 21 % (p < 0,05). Conclusion. The experimental culture medium for Listeria cultivation provided growth of colonies of the test strain L. monocytogenes 766 with the preservation of characteristic cultural, morphological and biochemical properties, and, in terms of germination and growth rate, exceeded the control media. The developed nutrient medium provides effective growth of Listeria and can be used as a medium for the accumulation of microbial mass.","PeriodicalId":6986,"journal":{"name":"Acta Biomedica Scientifica (East Siberian Biomedical Journal)","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85115373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HCV Infection in the Republic of Sakha (Yakutia) and Its Role of Genotypes in Clinical Outcomes of the Disease","authors":"V. K. Semenova, S. Sleptsova, S. Malov, E. Savilov, S. I. Semenov, I. Malov, L. A. Stepanenko, O. Ogarkov","doi":"10.29413/abs.2020-5.4.6","DOIUrl":"https://doi.org/10.29413/abs.2020-5.4.6","url":null,"abstract":"Aim of the research. To study the epidemic manifestations of HCV infection in the Republic of Sakha (Yakutia) in order to develop recommendations for improving the effectiveness and quality of treatment and prevention measures. Materials and methods. The paper uses materials from the official statistics of the Territorial Department of Rospotrebnadzor of the Republic of Sakha (Yakutia) for 1994–2018, and data from the electronic register «Chronic viral hepatitis in the RS (Ya)» (2019). Molecular and biological studies of the genotype of the hepatitis C virus were performed jointly on the basis of the Federal state budgetary Institution «Central research Institute of epidemiology» of Rospotrebnadzor (2007–2011, n = 75). To assess the epidemiological situation, the rate of increase in morbidity is calculated on the basis of data equalized by the method of least squares. Statistical processing was performed using the SPSS 17 program. The critical significance level is assumed to be 0.05. Results. Thus, the study of long-term dynamics of the incidence of viral hepatitis shows that in the Republic of Sakha (Yakutia) a consistently high level of incidence of HCV with adverse trends in the development of the epidemic process remains. Analysis of the distribution of different variants of HCV genotypes allowed us to establish the prevalence of genotype 1b, which can determine the high frequency of cirrhosis and primary liver cancer. The current situation in the Republic regarding the incidence of viral hepatitis requires detailed monitoring, improvement of epidemiological surveillance and introduction of modern treatment methods. It is also necessary to improve the quality of health education among the population of the Republic.","PeriodicalId":6986,"journal":{"name":"Acta Biomedica Scientifica (East Siberian Biomedical Journal)","volume":"155 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91491592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}