Acta Crystallographica Section A Foundations and Advances最新文献

Sample preparation for routine and advanced structural biology, including serial data collection, MicroED and cryo-EM 用于常规和高级结构生物学的样品制备，包括序列数据收集、显微电子显微镜和低温电子显微镜

Acta Crystallographica Section A Foundations and Advances Pub Date : 2023-07-07 DOI: 10.1107/s2053273323096420

Stefan A. Kolek, Patrick Shaw Stewart, Jack Stubbs, P. Baldock

{"title":"Sample preparation for routine and advanced structural biology, including serial data collection, MicroED and cryo-EM","authors":"Stefan A. Kolek, Patrick Shaw Stewart, Jack Stubbs, P. Baldock","doi":"10.1107/s2053273323096420","DOIUrl":"https://doi.org/10.1107/s2053273323096420","url":null,"abstract":"Serial data collection usually requires relatively small crystals that are well-ordered. Microseeding is an effective way to generate such samples. During the ten years since the random microseed matrix-screening (rMMS) method was published, understanding of the theoretical advantages of the method has increased [2 4], and several practical variations of the method have emerged. Moreover seeding can be carried out in a microbatch-underoil setup, which is easy to scale up, volume-wise, and allows easy interpretation of phase diagrams. By combining these techniques, control can be increased and sample quality for both routine and advanced data collection improved. Protein structure determination by cryoEM requires expensive equipment that has low throughput. It is therefore wasteful to examine samples that can be shown in advance to be aggregated, since such samples are unlikely to be suitable. We used a high-throughput screening approach with dynamic light scattering to explore 96 chemical conditions with as little as 10 μL of protein solution to identify conditions with reduced aggregation.","PeriodicalId":6903,"journal":{"name":"Acta Crystallographica Section A Foundations and Advances","volume":"198 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139361445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crystal structure of human interleukin-2 in complex with TCB2, a new antibody-drug candidate with antitumor activity 人白细胞介素-2 与 TCB2（一种具有抗肿瘤活性的候选抗体药物）复合物的晶体结构

Acta Crystallographica Section A Foundations and Advances Pub Date : 2023-07-07 DOI: 10.1107/s2053273323099023

Jieun Kim

引用次数: 0

From molecular to supramolecular to functional materials 从分子材料到超分子材料再到功能材料

Acta Crystallographica Section A Foundations and Advances Pub Date : 2023-07-07 DOI: 10.1107/s2053273323098534

Vinu V. Panikkattu, Viraj De Silvia, Christer Aakeroy

引用次数: 0

Analysis of human serum albumin metal complexes 人血清白蛋白金属复合物分析

Acta Crystallographica Section A Foundations and Advances Pub Date : 2023-07-07 DOI: 10.1107/s2053273323096183

Vanessa Bijak, Michael Gucwa, Michal Szcygiel, K. Handing, Wladek Minor

引用次数: 0

Increasing completeness in single-crystal high-pressure diffraction experiments by pre-orienting crystals 通过预定向晶体提高单晶高压衍射实验的完整性

Acta Crystallographica Section A Foundations and Advances Pub Date : 2023-07-07 DOI: 10.1107/s2053273323098078

D. Tchoń, Aleksandra Zwolenik, Anna M. Makal

引用次数: 0

Imaging neurons by cryo-electron tomography 利用低温电子断层扫描成像神经元

Acta Crystallographica Section A Foundations and Advances Pub Date : 2023-07-07 DOI: 10.1107/s2053273323098388

M. T. Swulius

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New cryo-EM methods to capture endogenous complexes in multiple functional states at atomic resolution 以原子分辨率捕捉多种功能态内源复合物的新型低温电子显微镜方法

Acta Crystallographica Section A Foundations and Advances Pub Date : 2023-07-07 DOI: 10.1107/s2053273323097991

Hong Zhou

{"title":"New cryo-EM methods to capture endogenous complexes in multiple functional states at atomic resolution","authors":"Hong Zhou","doi":"10.1107/s2053273323097991","DOIUrl":"https://doi.org/10.1107/s2053273323097991","url":null,"abstract":"The last decade has witnessed advances in high-resolution cryoEM “revolutionizing” structural biology; as such, cryoEM has become a highly sought-after means of biochemical and biomedical investigations. Results from cryoEM are beginning to signiﬁcantly enhance our understanding of the cellular processes responsible for maintenance, transmission and expression of genetic information at the atomic level. At the heart of these processes lie macromolecular complexes, within and outside the cell, which can now be studied by cryoEM. Understanding how and why these complexes function relies on visualizing their three-dimensional (3D) structures at their endogenous and multiple functional states. Towards this end, we have developed an integrative proteomics cryoEM methods to determine atomic structures of native cellular complexes, sub - particle reﬁnement and nucleic acid modeling methods to model genomic RNA and DNA in action. Of particular note, our cryoID method (Ho et al., Nat Methods, 2020) allows determination of atomic structures of endogenous complexes in cellular milieu, capturing their multiple states, including those in their acts of carrying out their functions. Applications of cryoID has enabled atomic structure determination of previously intractable biological systems from cellular milieu and membrane.","PeriodicalId":6903,"journal":{"name":"Acta Crystallographica Section A Foundations and Advances","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139361551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transitioning from academia to a cryo-EM CRO 从学术界过渡到冷冻电镜 CRO

Acta Crystallographica Section A Foundations and Advances Pub Date : 2023-07-07 DOI: 10.1107/s2053273323098789

Emily Armbruster

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Hydrogen-bonded frameworks for molecular structure determination 用于分子结构测定的氢键框架

Acta Crystallographica Section A Foundations and Advances Pub Date : 2023-07-07 DOI: 10.1107/s2053273323097656

Tony Hu

引用次数: 0

Optimizing data collection time for absolute configuration determination 优化数据采集时间，以确定绝对配置

Acta Crystallographica Section A Foundations and Advances Pub Date : 2023-07-07 DOI: 10.1107/s2053273323097255

Ashley Weiland

引用次数: 0