植物生理与分子生物学学报最新文献_第8页

Molecular identification of two new self-incompatible alleles (S-alleles) in Chinese pear (Pyrus bretschneideri). 中国梨两个新的自交不亲和等位基因(s -等位基因)的分子鉴定。

植物生理与分子生物学学报 Pub Date : 2007-02-01

Xiao-Feng Tan, Lin Zhang, Ta-Na Wuyun, De-Yi Yuan, Yu-Fen Cao, Ao-Fang Jiang, Wen-Jie Liang, Yan-Ling Zeng

{"title":"Molecular identification of two new self-incompatible alleles (S-alleles) in Chinese pear (Pyrus bretschneideri).","authors":"Xiao-Feng Tan, Lin Zhang, Ta-Na Wuyun, De-Yi Yuan, Yu-Fen Cao, Ao-Fang Jiang, Wen-Jie Liang, Yan-Ling Zeng","doi":"","DOIUrl":"","url":null,"abstract":"Self-incompatibility (SI) is an important intraspecific reproductive barrier in flowering plants. To identify the S-alleles of Chinese pear species (Pyrus pyrifolia, P. bretschneideri, P. ussuriensis and P. sinkiangenis etc.), S-RNase-specific PCR amplification, sequence analyses and field pollination tests were performed using two cultivars 'Jingxiang' and 'Esu' of P. bretschneideri as materials. Two new S-RNase genes were identified from the two cultivars. They were 1,122 bp and 1,058 bp in length, and designated as S37-RNase (GenBank accession no. DQ839238) and S38-RNase (GenBank accession no. DQ839239). By comparison of their deduced amino acid sequences with those of S1-to S36-alleles of Oriental pear, it can be seen that both the two new S-alleles had their conserved regions C1 and C2, but their hypervariable regions (HV) were quite different from those of the others. S37 showed a higher similarity (96%) to S38 in the amino acid sequences deduced from them, whereas both of them displayed the highest similarity (98%) to S15 and the lowest (63%) to S32. The two S-alleles had introns of 786 bp and 723 bp, respectively, similar in size to that of S15 (777 bp). Finally, the S-genotypes of 'Jinxiang' and 'Esu' were unambiguously determined as S34S37 and S15S38, respectively.","PeriodicalId":64030,"journal":{"name":"植物生理与分子生物学学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26542282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[An analysis of genetic diversity of different ecotypes of reed (Phragmites communis Trin.) by molecular marker techniques]. 芦苇(Phragmites communis Trin.)不同生态型遗传多样性的分子标记分析

植物生理与分子生物学学报 Pub Date : 2007-02-01

Wen-Fang Lin, Lin-Jiao Chen, Xue-Yi Zhu

引用次数: 0

[Relationship between chloride tolerance and polyamine accumulation in Glycine max, Glycine soja, and their hybrid seedlings]. [Glycine max、Glycine soja 及其杂交幼苗耐氯化物能力与多胺积累之间的关系]。

植物生理与分子生物学学报 Pub Date : 2007-02-01

Xuan-Qin Chen, Bing-Jun Yu, You-Liang Liu

{"title":"[Relationship between chloride tolerance and polyamine accumulation in Glycine max, Glycine soja, and their hybrid seedlings].","authors":"Xuan-Qin Chen, Bing-Jun Yu, You-Liang Liu","doi":"","DOIUrl":"","url":null,"abstract":"The seedlings of the F4 hybrid strain 'JB185' selected for salt tolerance generation by generation, their parents Glycine max cv. Jackson and Glycine soja population 'BB52' were treated with different NaCl concentrations and iso-osmotic (-0.53 MPa) PEG-6000, NaCl, Na+ (without Cl-) and Cl- (without Na+) solutions for 6 d. The results showed that: (1) The relative electrolyte leakage and malondialdehyde (MDA) content in leaves of the above three soybean seedlings showed an increase trend when the NaCl concentration was elevated, but chlorophyll contents decreased except the significant increase in 'BB52' and 'JB185' under NaCl 50 mmol/L stress. The change in 'JB185' was between its parents. (2) Under different iso-osmotic stresses, the relative electrolyte leakage and MDA contents in leaves of three soybean seedlings also increased mostly, the changes in 'BB52' and 'JB185' under Na+ (without Cl-) stress were more than those under Cl- (without Na+) stress. The free and bound Put, Spd and Spm contents in leaves all increased when compared with the control, the ratios of free (Spd+Spm)/Put and total bound polyamines in 'BB52' and 'JB185' seedlings under Na+ (without Cl-) treatment were the lowest one among three iso-osmotic salt stresses. The results indicate that the F4 hybrid strain 'JB185' is more sensitive to Na+ than Cl- as its wild parent 'BB52' population.","PeriodicalId":64030,"journal":{"name":"植物生理与分子生物学学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26542280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequential amplification of flanking sequences by Y-shaped adaptor dependent extension using multiple templates. 利用多模板y形接头依赖扩展对侧翼序列进行顺序扩增。

植物生理与分子生物学学报 Pub Date : 2007-02-01

Yue-Hua Xiao, Yi Peng, Ming Luo, De-Mou Li, Lei Hou, Yan Pei

引用次数: 0

[Construction and preliminary analysis of subtractive library for peel pitting of 'Fengjie' navel orange fruit]. [‘奉节’脐橙果皮点蚀减法文库的构建及初步分析]。

植物生理与分子生物学学报 Pub Date : 2007-02-01

Xue Gao, Zheng-Guo Li, Jin Fan, Ying-Wu Yang

{"title":"[Construction and preliminary analysis of subtractive library for peel pitting of 'Fengjie' navel orange fruit].","authors":"Xue Gao, Zheng-Guo Li, Jin Fan, Ying-Wu Yang","doi":"","DOIUrl":"","url":null,"abstract":"In this study, the pitted peel and non-pitted peel of 'Fengjie' navel orange fruits were used as experimental materials to construct and screen the peel pitting related genes by suppression subtractive hybridization (SSH). The results showed that suppression subtractive hybridization was very effective. A cDNA library of differentially expressed genes was constructed. The library included about 200 clones with an average insert size of around 300 bp. Part of the positive clones were picked up randomly and sequenced. Six of the 50 clones had no homologous sequences being found and three had unknown functions in GenBank. According to the analysis of the homology, four homologous (Ca2+ binding protein, cysteine proteinase, NAC-domain protein and expansin) genes were chosen to examine their expressions through semi-quantitative RT-PCR analysis in pitted and non-pitted navel orange fruits. The expression of four genes were all higher in pitted peel than that in non-pitted peel. It suggests that these genes in the SSH cDNA library may be involved with peel pitting and can be subject of future investigation to explore the molecular biological mechanism of the pitting of citrus fruit.","PeriodicalId":64030,"journal":{"name":"植物生理与分子生物学学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26542283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in proteins within germinating seeds of transgenic wheat with an antisense construct directed against the thioredoxin. 反硫氧还蛋白转基因小麦种子萌发过程中蛋白质的变化。

植物生理与分子生物学学报 Pub Date : 2007-02-01

Hong-Xiang Guo, Jun Yin, Jiang-Ping Ren, Zhen-Yun Wang, Huan-Li Chen

引用次数: 0

[Physiological function of arginine and its metabolites in plants]. [精氨酸及其代谢物在植物中的生理功能]。

植物生理与分子生物学学报 Pub Date : 2007-02-01

Hong-Qiang Yang, Hua-Jun Gao

引用次数: 0

Histological observation of somatic embryogenesis from cultured embryos of Quercus variabilis Bl. 栓皮栎胚体胚发生的组织学观察。

植物生理与分子生物学学报 Pub Date : 2007-02-01

Cun-Xu Zhang, Zeng-Yu Yao, Zhong Zhao, Jian-Hua Qi

{"title":"Histological observation of somatic embryogenesis from cultured embryos of Quercus variabilis Bl.","authors":"Cun-Xu Zhang, Zeng-Yu Yao, Zhong Zhao, Jian-Hua Qi","doi":"","DOIUrl":"","url":null,"abstract":"Immature zygotic embryos of Quercus variabilis Bl. were excised and cultured on MS basal medium containing 0.25 mg/L 2,4-dichlorophenoxyacetic acid and 0.5 mg/L 6-benzyl aminopurine. Callus was initiated from these embryos within 6 weeks. Two types of embryogenic calluses were formed: one was the white mucilaginous callus and the other was light yellow to translucent, glossy, mucilaginous. Histological examination showed that the non-embryogenic cells derived from zygotic embryos were large in size, with small nuclei, thin cytoplasm, and the embryogenic cells were small in size, with large nuclei, thick cytoplasm and denser arrangement. Somatic embryos were induced from embryogenic masses (EMS) cultured on a medium not containing plant growth regulators (PGRs). The histological origin of somatic embryos was single epidermal or subepidermal cells. Somatic embryos displayed a standard development pattern, from globular ones to heart-like and torpedo-shaped ones and finally to embryo with two cotyledons, which was similar to the development of zygotic embryo in vivo. Secondary proembryos were formed on the axis and cotyledon of existing embryos and originated from single, densely stained cells of the epidermis at all stages.","PeriodicalId":64030,"journal":{"name":"植物生理与分子生物学学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26542278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution changes of calcium and programmed cell death in the pistil of litchi (Litchi chinensis Sonn.) flower during its development. 荔枝(litchi chinensis Sonn.)花发育过程中雌蕊中钙的分布变化及细胞程序性死亡。

植物生理与分子生物学学报 Pub Date : 2006-12-01

Xiang-Ping Wang, Li-Xun Su, Jin-Wei Su

{"title":"Distribution changes of calcium and programmed cell death in the pistil of litchi (Litchi chinensis Sonn.) flower during its development.","authors":"Xiang-Ping Wang, Li-Xun Su, Jin-Wei Su","doi":"","DOIUrl":"","url":null,"abstract":"Potassium pyroantimonate precipitation method was used for investigating calcium distribution and cell ultrastructure change during development of pistils of litchi male and female flower. The results showed that at the megasporocyte stage of female flowers, calcium precipitates was located mainly at cell wall and intercellular space of inner integument near the micropyle and style cells, and to a lesser extent in vacuoles. Vascular tissues also contained much calcium precipitates. In inner integument cells near the micropyle of male flowers, the vacuole contained most of the calcium precipitates. Calcium precipitates in style cell and vascular tissues of male flowers was sparse and seldom seen. After meiosis of megasporocyte, pistils of female flowers continued to grow and those of male flowers aborted. In female flowers, calcium precipitates concentration became lower and calcium precipitates was probably transported to the places for future pollen bourgeoning and fertilization. Cell wall calcium precipitates concentration increased in the inner integument cells near the micropyle. Calcium precipitates concentration increased from topper style cells to lower ones. In male flowers, inner integument cells near the micropyle underwent the programmed cell death (PCD): flow of calcium from vacuoles into nucleus might had triggered the PCD process. A continuous channel was formed between perinuclear space and cytoplasm membrane lumen, and calcium flowed freely between nuclear membrane and plasma membrane. At certain time and locations, calcium precipitates was newly appeared at some organelles like endoplasimic reticulum, mitochondria and peroxisomes. This calcium redistribution in cells might trigger and regulate the process of PCD. In male flowers, style cells containing no calcium precipitation soon began to degenerate.","PeriodicalId":64030,"journal":{"name":"植物生理与分子生物学学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26441373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Adaptive changes in activity of tonoplast H(+)-ATPase from the roots of tomato seedlings under phosphorus starvation]. [缺磷条件下番茄根系叶绿体H(+)- atp酶活性的适应性变化]。

植物生理与分子生物学学报 Pub Date : 2006-12-01

Shu-Hua Shan, Ke-Min Song, Jing-Ru Liu, Han-Bing Xu

{"title":"[Adaptive changes in activity of tonoplast H(+)-ATPase from the roots of tomato seedlings under phosphorus starvation].","authors":"Shu-Hua Shan, Ke-Min Song, Jing-Ru Liu, Han-Bing Xu","doi":"","DOIUrl":"","url":null,"abstract":"We studied the growth state of tomato seedlings and the adaptive changes in activity of tonoplast H(+)-ATPase on different days (5 d, 7 d, 9 d, 11 d, 13 d) sampling from the roots of tomato seedlings under phosphorus starvation. The results indicated that the average height of the seedlings decreased under phosphorus starvation, while the main roots of the seedlings were significantly longer than those of the control. The phosphorus uptake rates of tomato seedlings under phosphorus starvation significantly increased and were much higher than those of controls and reached maximum values under the starvation for 7 days. The tonoplast H(+)-ATPase activity in the roots of tomato seedlings increased under phosphorus starvation and reached maximum values under the starvation for 7 days, while the activity of the control changed little. Kinetic analysis of tonoplast H(+)-ATPase showed that phosphorus starvation significantly lowered the K(m) value, but had no significant effect on the V(max) value of the enzyme. The results showed that phosphorus starvation increased the affinity of the enzyme to its substrate. In addition, phosphorus starvation did not alter the optimum pH (7.5) of the ATPase activity.","PeriodicalId":64030,"journal":{"name":"植物生理与分子生物学学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26442060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0