植物生理与分子生物学学报最新文献_第5页

Time course of expression of chalcone synthase gene in Ginkgo biloba. 银杏查尔酮合成酶基因表达的时间过程。

植物生理与分子生物学学报 Pub Date : 2007-08-01

Feng Xu, Shui-Yuan Cheng, Shu-Han Cheng, Yan Wang, He-Wei Du

引用次数: 0

The involvement of NtFtsZ2-1 gene in the regulation of chloroplast division and expansion in tobacco. NtFtsZ2-1 基因参与调控烟草叶绿体的分裂和扩展。

植物生理与分子生物学学报 Pub Date : 2007-08-01

Wei-Zhong Liu, Dong-Dong Kong, Dong Wang, Chuan-Li Ju, Yong Hu, Xiang-Lin Liu, Jing-San Sun, Yi-Kun He

{"title":"The involvement of NtFtsZ2-1 gene in the regulation of chloroplast division and expansion in tobacco.","authors":"Wei-Zhong Liu, Dong-Dong Kong, Dong Wang, Chuan-Li Ju, Yong Hu, Xiang-Lin Liu, Jing-San Sun, Yi-Kun He","doi":"","DOIUrl":"","url":null,"abstract":"Chloroplasts are a vital group of organelles of plants, yet the molecular mechanisms associated with their division remain poorly understood. Recent studies have revealed that the FtsZ protein, known as a key component in prokaryotic cell division, is involved in chloroplast division process. The NtFtsZ2-1 gene was isolated from Nicotiana tabacum by RT-PCR, and the sense and antisense expression plasmids were used to examine the function of NtFtsZ2-1 gene in transgenic tobacco. Light and confocal observations revealed that the normal chloroplast division process was severely disrupted in transgenic plants with enhanced or reduced expression of NtFtsZ2-1 gene. These chloroplasts were abnormally larger in size and fewer in number compared with that of the wild-type tobacco. But the total chloroplast plan area per mesophyll cell was conserved in sense, antisense and wild type tobaccos. Analyses of electron micrographs and chlorophyll content of different transgenic plants showed that constitutively enhancing or inhibiting the expression of NtFtsZ2-1 gene had no direct influence on the ultrastructure and photosynthetic ability of chloroplasts. Basing on these results, we suggest that NtFtsZ2-1 gene is involved in chloroplast division and expansion; the fluctuation of NtFtsZ2-1 expression level would alter normal chloroplast number and size in plant cells. In addition, the similarities of ultrastructure and photosynthetic ability of chloroplasts among sense, antisense and wild type tobaccos implies that a special mechanism regulate the relationship between chloroplast number and size to maximize photosynthetic rate.","PeriodicalId":64030,"journal":{"name":"植物生理与分子生物学学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26870354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cadmium-induced membrane lipid peroxidation and changes in antioxidant enzyme activities and peroxidase isoforms in Jerusalem artichoke seedlings. 镉诱导的耶路撒冷洋蓟幼苗膜脂过氧化及抗氧化酶活性和过氧化物酶同工型的变化。

植物生理与分子生物学学报 Pub Date : 2007-08-01

Yi-Ming Tao, Yan-Zhen Chen, Yang-Lin Liang, Mei-Yan Xu, Xiang-Ming Xu

{"title":"Cadmium-induced membrane lipid peroxidation and changes in antioxidant enzyme activities and peroxidase isoforms in Jerusalem artichoke seedlings.","authors":"Yi-Ming Tao, Yan-Zhen Chen, Yang-Lin Liang, Mei-Yan Xu, Xiang-Ming Xu","doi":"","DOIUrl":"","url":null,"abstract":"Jerusalem artichoke (Helianthus tuberosus L.) seedlings cultured in sandy media were treated with Hoagland nutrition solution with different concentrations of Cd(NO(3))(2) from 0 to 400 micromol/L. After 50 days' treatment, Cd accumulation, activities of peroxidase (POD, EC 1.11.1.7), superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6) were measured and electrophoretograms of POD isoenzymes were analyzed. The accumulation of Cd in seedlings increased from Cd 50-100 micromol/L, after which further increases in Cd concentration resulted in only small increases in accumulation of Cd in seedlings. MDA content was markedly higher than control values indicating the enhanced membrane lipid peroxidation in roots and leaves. POD activities in leaf and root extracts increased with an increase of Cd concentration from 0 to 50 and 100 micromol/L and then decreased with further increases to 200 and 400 micromol/L. Under moderate Cd level of 50-200 micromol/L, SOD activities in leaf and root extracts increased whereas with a higher Cd level of 400 micromol/L marked inhibitions in enzyme activities were observed. With increase in Cd concentration marked elevations in CAT activities in leaves and roots were observed. Results of electrophoresis show that the alteration of POD isoenzyme was noticeable to Cd and an additional POD isoenzyme LP10 appeared. It is suggested that POD isoenzyme of Jerusalem artichoke seedlings could be used as bioindicator for soil contamination by Cd.","PeriodicalId":64030,"journal":{"name":"植物生理与分子生物学学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26870363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between grain yield and leaf photosynthetic rate in super hybrid rice. 超级杂交稻籽粒产量与叶片光合速率的关系

植物生理与分子生物学学报 Pub Date : 2007-06-01

Yue Chen, Long-Ping Yuan, Xue-Hua Wang, Dao-Yun Zhang, Juan Chen, Qi-Yun Deng, Bing-Ran Zhao, Da-Quan Xu

{"title":"Relationship between grain yield and leaf photosynthetic rate in super hybrid rice.","authors":"Yue Chen, Long-Ping Yuan, Xue-Hua Wang, Dao-Yun Zhang, Juan Chen, Qi-Yun Deng, Bing-Ran Zhao, Da-Quan Xu","doi":"","DOIUrl":"","url":null,"abstract":"In order to explore the relationship between grain yield and photosynthesis, the yield composition and leaf photosynthetic rate in some super hybrid rices and ordinary hybrid rice 'Shanyou 63' as control were measured in 2000-2005. The results were as follows. (1) The yield levels of the four super hybrid rices, 'Pei'ai 64S/E32', 'P88S/0293', 'Jin23A/611' and 'GD-1S/RB207', were significantly higher, being 108%-120% of 'Shanyou 63'. (2) These super hybrid rices had a better plant type with more erect upper layer leaves and bigger panicles or more spikelets per panicle, being 125%-177% of spikelets Shanyou 63 spikelets. (3) Net photosynthetic rates of these super hybrid rices were significantly higher in the second leaf but not necessarily in the first leaf or flag leaf than those of spikelets Shanyou 63 spikelets. (4) The removal of half flag leaf led to a decline in the seed-setting rate, while the removal of half panicle induced its increase in spikelets GD-1S/RB207 spikelets. Hence, higher yield in these super hybrid rices can be attributed to their bigger panicles, better plant type and higher light use efficiency of their canopies. Raising the photosynthetic capacity of each leaf, especially flag leaf, is the key to overcome the photosynthate-source restriction on grain yield and to make a new breakthrough of yield potential in future breeding of super hybrid rice.","PeriodicalId":64030,"journal":{"name":"植物生理与分子生物学学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26766884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The control of sugar accumulation within strawberry aggregate fruit by invertase and hexokinase]. 转化酶和己糖激酶对草莓果实中糖积累的控制

植物生理与分子生物学学报 Pub Date : 2007-06-01

Ming Xie, Jun-Wei Chen, Qiao-Pin Qin, Gui-Hua Jiang, Chong-Bo Sun, Hui-Qin Zhang, Hong-Xia Xu

{"title":"[The control of sugar accumulation within strawberry aggregate fruit by invertase and hexokinase].","authors":"Ming Xie, Jun-Wei Chen, Qiao-Pin Qin, Gui-Hua Jiang, Chong-Bo Sun, Hui-Qin Zhang, Hong-Xia Xu","doi":"","DOIUrl":"","url":null,"abstract":"The glucose, fructose and sucrose contents and the activities of invertase, sucrose synthase (SS), sucrose phosphate synthase (SPS), hexokinase (HXK) and fructokinase (FRK) of 'Tochiotome' strawberry aggregate fruit were analyzed at various stages of fruit development. The sugar accumulation exhibited the difference between various parts of the fruit. The sugar content is the highest in the top part of ripe 'Tochiotome' strawberry aggregate fruit, followed by the central part, and the basal part was the lowest. Changes in activity of invertase corresponded to the changes of sugar concentration gradient within various parts of the ripe strawberry aggregate fruit. The SS and SPS activities within various parts of aggregate fruit did not showed obvious change that corresponded to the sugar content. At the stage of fruit maturation, the HXK and FRK activities within various parts of strawberry aggregate fruit, however, exhibited an opposite gradient to sugar content. The basal part of aggregate fruit had the highest activities of HXK and FRK, while the top part had the lowest activities. These results suggested that the higher activity of invertase in the top part of aggregate fruit may play a role for rapid cleavage of sucrose and help to form a sucrose gradient in apoplastic space from the basal part to the top part of strawberry aggregate fruit, thus enhance photosynthate translocation from the basal part to the top part of aggregate fruit, which led to higher sugar accumulation in the top part of fruit. In the basal part of aggregate fruit, the higher activities of hexose-metabolizing enzymes may promote the consumption of hexose, and thus resulted in the lowering of sugar content in the basal part than in the top part of strawberry aggregate fruit.","PeriodicalId":64030,"journal":{"name":"植物生理与分子生物学学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26766936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A calcium-dependent protein kinase is involved in plant hormone signal transduction in Arabidopsis. 一种钙依赖性蛋白激酶参与拟南芥植物激素信号转导。

植物生理与分子生物学学报 Pub Date : 2007-06-01

Xin Yuan, Ke-Qin Deng, Xiao-Ying Zhao, Xian-Jin Wu, Yu-Zhi Qin, Dong-Ying Tang, Xuan-Ming Liu

{"title":"A calcium-dependent protein kinase is involved in plant hormone signal transduction in Arabidopsis.","authors":"Xin Yuan, Ke-Qin Deng, Xiao-Ying Zhao, Xian-Jin Wu, Yu-Zhi Qin, Dong-Ying Tang, Xuan-Ming Liu","doi":"","DOIUrl":"","url":null,"abstract":"A number of signal pathways have been found through which abundant calcium-stimulated protein kinase activity in plant is associated with calcium-dependent protein kinases (CDPKs) which act as the calcium sensors mediating numerous responses, including hormone signaling. Basing on previous studies, we made additional functional analysis of the gene AtCPK30 encoding a protein kinase in Arabidopsis. Results of semi-quantitative reverse transcription PCR (RT-PCR) analysis indicated that AtCPK30 was highly expressed in root and induced by ABA, IAA, 2,4-D, GA(3) and 6-BA treatment. The physiological roles of AtCPK30 were studied using a gain-of-function approach. Seedlings of AtCPK30 transgenic lines had longer primary roots than those plants of wild-type at the early stages. Interestingly, when these plants grew on MS lack of Ca(2+) including wild-type and transgenic lines, the roots of transgenic line were more sensitive to calcium, lack of Ca(2+) had less effect on roots of transgenic lines than those of wild-type. Treated with several plant hormones, such as ABA, IAA, GA(3) and 6-BA, the roots of seedlings of transgenic line developed abnormally because they were more sensitive to hormones. Furthermore, NPA relatively less inhibited emergency of lateral roots of transgenic line than those of the wild-type. Green fluorescent protein-CPK30 (GFP-CPK30) fusion protein studies revealed the localization of AtCPK30 to both cell wall and plasma membrane. These results suggest that AtCPK30 acts as the calcium sensor and involved in the hormone-signaling pathways.","PeriodicalId":64030,"journal":{"name":"植物生理与分子生物学学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26766938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning and expression of the chloroplast copper/zinc-superoxide dismutase gene in upland cotton (Gossypium hirsutum L.). 陆地棉叶绿体铜锌超氧化物歧化酶基因的克隆与表达。

植物生理与分子生物学学报 Pub Date : 2007-06-01

Gen-Hai Hu, Shu-Xun Yu, Shu-Li Fan, Mei-Zhen Song

{"title":"Cloning and expression of the chloroplast copper/zinc-superoxide dismutase gene in upland cotton (Gossypium hirsutum L.).","authors":"Gen-Hai Hu, Shu-Xun Yu, Shu-Li Fan, Mei-Zhen Song","doi":"","DOIUrl":"","url":null,"abstract":"A full-length 1,043-base-pair cDNA clone encoding a chloroplast copper/zinc superoxide dismutase (Cu/Zn-SOD) of upland cotton was first isolated by rapid amplification of cDNA ends (RACE) from the leaves of the cotton (Gossypium hirsutum L.) variety 'CRI36'. Nucleotide sequence analysis of the clone revealed that it contained the complete coding sequence of the mature SOD isozyme subunit, along with a 60-amino acid transit peptide at N-terminal. The amino acid sequence predicted from the full-length clone showed 66%-74% homology with the amino acid sequences of Cu/Zn-SOD from several other plants. This gene was found to be expressed in the leaves and stems, but not in roots, flowers, and hypocotyls, indicating that the gene was expressed only in green tissues. Also, its expression was found to be most active at seedling stage and declined gradually in later development stages. Expression of this cotton Cu/Zn-SOD gene by using the pET-21a (+) expression vector in E. coli BL21 (DE3) led to the production of a novel 29 kD polypeptide with SOD enzyme activity, confirming that the cloned cotton Cu/Zn-SOD cDNA was indeed encoding a functioning SOD enzyme.","PeriodicalId":64030,"journal":{"name":"植物生理与分子生物学学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26766933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Expression of the GUS fusion gene controlled by the tomato rbcS3A promoter in transgenic rice]. [番茄rbcS3A启动子调控GUS融合基因在转基因水稻中的表达]。

植物生理与分子生物学学报 Pub Date : 2007-06-01

Qiao-Quan Liu, Heng-Xiu Yu, Wen-Juan Zhang, Zhi-Yun Gong, Ming-Hong Gu

{"title":"[Expression of the GUS fusion gene controlled by the tomato rbcS3A promoter in transgenic rice].","authors":"Qiao-Quan Liu, Heng-Xiu Yu, Wen-Juan Zhang, Zhi-Yun Gong, Ming-Hong Gu","doi":"","DOIUrl":"","url":null,"abstract":"To use different types of promoters in transgenic rice research, the 1.1 kb 5'-upstream regulation region of one of the tomato (Solanum tuberosum L.) Rubisco small subunit gene, rbcS3A, was cloned and its sequences were confirmed by comparison with the known sequences in GenBank. The cloned rbcS3A promoter was fused to the 5'-upstream of GUS (beta-glucuronidase) coding region in a binary vector, and introduced into an elite japonica rice variety by Agrogacterium-mediated transformation. The integration of the GUS fusion gene into the genome of transgenic rice was confirmed by both PCR and Southern blot analysis. The results of both histochemical staining and quantitative analysis of GUS activity showed that the expression level of GUS fusion gene was significantly stronger in stem, leaf blade and sheath than in other organs of transgenic rice plants, and showed highest in the stem, which implies that the tomato rbcS3A promoter can make tissue-specific, in particular in the stem, expression of foreign genes in transgenic rice. The results present here also demonstrate that light induction had no effect on the expression of the foreign gene when regulated by the tomato rbcS3A promoter in transgenic rice. Our results show that the cloned tomato rbcS3A promoter might be very useful for the expression of target genes in transgenic rice, with particularly high efficiency in stem tissues.","PeriodicalId":64030,"journal":{"name":"植物生理与分子生物学学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26766887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The application of two-dimensional liquid phase chromatography to separation of total protein of Leymus chinensis shoot]. 二维液相色谱法在羊草芽总蛋白分离中的应用

植物生理与分子生物学学报 Pub Date : 2007-06-01

Li-Nan Xie, Yu-Zhe Nie, Xiao-Lei Zhang, Teng-Xiang Chen, Jing-Hua Liu, Yong Jiang, Yu-Hua Li

{"title":"[The application of two-dimensional liquid phase chromatography to separation of total protein of Leymus chinensis shoot].","authors":"Li-Nan Xie, Yu-Zhe Nie, Xiao-Lei Zhang, Teng-Xiang Chen, Jing-Hua Liu, Yong Jiang, Yu-Hua Li","doi":"","DOIUrl":"","url":null,"abstract":"The total proteins of Leymus chinensis were extracted from eight-week-old seedlings grown in the greenhouse by TCA-acetone precipitation method. After the lysis buffer was replaced by the start buffer, proteins were fractionated along the first dimension using chromatofocusing (CF). Subsequently the fractions with pI values between 8.5 and 4.0 collected after the first dimension separation were further fractionated by nonporous silica reverse-phase high-performance liquid chromatography (NPS-RP-HPLC, HPRP). With ProteoVue software the pI/UV map was generated to show the protein expression profile of Leymus chinensis. Some experiments were tested to optimize the fraction procedure. Three different elution gradients were employed to get the optimal chromatogram. Comparison of the protein expression pattern between 2D-PAGE and 2D-LC indicated that 2D-LC was a powerful tool in protein fraction. Reproducibility and veracity of the protein patterns were confirmed in different injections of the same sample. A method to fractionate the total protein of Leymus chinensis shoot with two-dimensional liquid chromatography (2D-LC) was founded.","PeriodicalId":64030,"journal":{"name":"植物生理与分子生物学学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26766888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Analysis of genomic DNA methylation level in radish under cadmium stress by methylation-sensitive amplified polymorphism technique]. [甲基化敏感扩增多态性技术分析镉胁迫下萝卜基因组DNA甲基化水平]。

植物生理与分子生物学学报 Pub Date : 2007-06-01

Jin-Lan Yang, Li-Wang Liu, Yi-Qin Gong, Dan-Qiong Huang, Feng Wang, Ling-Li He

引用次数: 0