干细胞探索(英文) Pub Date : 2023-01-01 DOI: 10.4236/scd.2023.131001

A. Ismail, S. Abdou, A. Yousef, Yousra Sameh, M. Attia, A. Badran, Mohamed I. Adel El Eissawy, Asmaa E. Bedeer, W. Salama, Ahmed O. Korany

引用次数: 1

Stem cell and Non-stem Cell Components of Breast Milk 母乳中的干细胞和非干细胞成分

干细胞探索(英文) Pub Date : 2023-01-01 DOI: 10.1007/978-981-99-0647-5

I. Somasundaram, P. Kaingade, R. Bhonde

引用次数: 0

Stem Cells: A Promising Therapeutic Target for COVID-19 干细胞:治疗COVID-19的一个有希望的靶点

干细胞探索(英文) Pub Date : 2022-01-01 DOI: 10.4236/scd.2022.121001

Leyla Tahrani Hardin, Nan Xiao

引用次数: 1

Isolation and Ex Vivo Expansion of Human Hematopoietic Stem Cells Derived from Umbilical Cord Blood 人脐带血造血干细胞的分离与体外扩增

干细胞探索(英文) Pub Date : 2021-01-05 DOI: 10.4236/SCD.2021.111001

Mao Pang, Shiqian Han, M. Ren, Dongwei Guan, Tianying Kuang, Shuixia Liu, Yesong Liu, Jieping Chen, Fengchao Wang, C. Shi, Zhijia Ye

{"title":"Isolation and Ex Vivo Expansion of Human Hematopoietic Stem Cells Derived from Umbilical Cord Blood","authors":"Mao Pang, Shiqian Han, M. Ren, Dongwei Guan, Tianying Kuang, Shuixia Liu, Yesong Liu, Jieping Chen, Fengchao Wang, C. Shi, Zhijia Ye","doi":"10.4236/SCD.2021.111001","DOIUrl":"https://doi.org/10.4236/SCD.2021.111001","url":null,"abstract":"Umbilical cord blood (UCB) is a current major source of hematopoietic \u0000stem cells (HSCs) for cell transplantation therapy. Cell transplantation with \u0000HSCs derived from UCB is advantageous over transplantation with HSCs from adult \u0000tissues. However, the low number of HSC derived from a single unit of UCB \u0000limits its application. Thus, ex vivo expansion is a good option to create more UCB HSCs for clinical application. \u0000The strategies for HSC expansion in vitro focus on mimicking the composition and structure of HSC natural niche by \u0000enhancing self-renewal and inhibiting lineage differentiation of HSCs. In the \u0000past decade, the mechanisms of the interaction between HSC and the natural \u0000niche have been deeply investigated. This great progress in basic research has \u0000led to advancements in UCB HSC ex vivo expansion. In addition, the biological characteristics of the originally isolated UCB \u0000HSCs correlate with outcome of subsequent ex vivo expansion. In this paper, we summarize the late progress achieved \u0000in isolation and ex vivo expansion of \u0000UCB HSCs. Importantly, we attempt to provide an impact and practicable \u0000procedure to expand UCB HSC in vitro from isolation of original HSCs to identification of expanded HSCs.","PeriodicalId":60944,"journal":{"name":"干细胞探索(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42878731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Chicken Mesenchymal Stem Cells as Feeder Cells Facilitate the Cultivation of Primordial Germ Cells from Circulating Blood and Gonadal Ridge 鸡间充质干细胞作为饲养细胞有利于循环血液和性腺嵴中原始生殖细胞的培养

干细胞探索(英文) Pub Date : 2019-01-31 DOI: 10.4236/SCD.2019.91001

L. Dongsheng, Chen Zhisheng, Sheng‐feng Chen, Ji Huiqin, Zhan Xiaoshu, Luo Dongzhang, Luo Huina, Wang Bingyun

{"title":"Chicken Mesenchymal Stem Cells as Feeder Cells Facilitate the Cultivation of Primordial Germ Cells from Circulating Blood and Gonadal Ridge","authors":"L. Dongsheng, Chen Zhisheng, Sheng‐feng Chen, Ji Huiqin, Zhan Xiaoshu, Luo Dongzhang, Luo Huina, Wang Bingyun","doi":"10.4236/SCD.2019.91001","DOIUrl":"https://doi.org/10.4236/SCD.2019.91001","url":null,"abstract":"Long-term maintenance of chicken primordial germ cells (PGCs) in vitro has tremendous potential for transgenic chicken production. Feeder cells are essential for the establishment and culture of chicken PGCs in vitro. Buffalo rat liver (BRL) cells are the most commonly used feeder cells for PGCs culture; however, this feeder layers from other animal species usually cause immunogenic contaminations, compromising the potential of PGCs in applications. Therefore, we tested chicken source mensenchymal stem cell (MSCs) derived from bone marrow as feeder cells to further improve PGC culture conditions. MSCs derived from chicken bone marrow have a powerful capacity to proliferate and secrete cytokines. We found chicken primordial germ cells derived from circulating blood (cPGCs) and gonads (gPGCs) can be maintained and proliferated with MSCs feeder layer cells. PGCs co-cultured on MSCs feeder retained their pluripotency, expressed PGCs specific genes and stemness markers, and maintained undifferentiated state. Our study indicated that the xeno-free MSCs-feeders culture system is a good candidate for growth and expansion of PGCs as the stepping stone for transgenic chicken research.","PeriodicalId":60944,"journal":{"name":"干细胞探索(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47252678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Stem Cell Renewal and Cell-Cell Communication: Methods and Protocols 干细胞更新和细胞间通讯:方法和协议

干细胞探索(英文) Pub Date : 2015-01-01 DOI: 10.1007/978-1-0716-1570-6

引用次数: 1

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