Chicken Mesenchymal Stem Cells as Feeder Cells Facilitate the Cultivation of Primordial Germ Cells from Circulating Blood and Gonadal Ridge

干细胞探索(英文) Pub Date : 2019-01-31 DOI:10.4236/SCD.2019.91001

L. Dongsheng, Chen Zhisheng, Sheng‐feng Chen, Ji Huiqin, Zhan Xiaoshu, Luo Dongzhang, Luo Huina, Wang Bingyun

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引用次数: 4

Abstract

Long-term maintenance of chicken primordial germ cells (PGCs) in vitro has tremendous potential for transgenic chicken production. Feeder cells are essential for the establishment and culture of chicken PGCs in vitro. Buffalo rat liver (BRL) cells are the most commonly used feeder cells for PGCs culture; however, this feeder layers from other animal species usually cause immunogenic contaminations, compromising the potential of PGCs in applications. Therefore, we tested chicken source mensenchymal stem cell (MSCs) derived from bone marrow as feeder cells to further improve PGC culture conditions. MSCs derived from chicken bone marrow have a powerful capacity to proliferate and secrete cytokines. We found chicken primordial germ cells derived from circulating blood (cPGCs) and gonads (gPGCs) can be maintained and proliferated with MSCs feeder layer cells. PGCs co-cultured on MSCs feeder retained their pluripotency, expressed PGCs specific genes and stemness markers, and maintained undifferentiated state. Our study indicated that the xeno-free MSCs-feeders culture system is a good candidate for growth and expansion of PGCs as the stepping stone for transgenic chicken research.

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鸡间充质干细胞作为饲养细胞有利于循环血液和性腺嵴中原始生殖细胞的培养

鸡原始生殖细胞（PGCs）在体外的长期维持对转基因鸡的生产具有巨大的潜力。饲养细胞对于体外建立和培养鸡PGCs是必不可少的。水牛大鼠肝脏（BRL）细胞是PGCs培养中最常用的饲养细胞；然而，这种来自其他动物物种的饲养层通常会引起免疫原性污染，从而损害PGCs在应用中的潜力。因此，我们测试了来源于骨髓的鸡源间充质干细胞（MSC）作为饲养细胞，以进一步改善PGC培养条件。来源于鸡骨髓的间充质干细胞具有强大的增殖和分泌细胞因子的能力。我们发现来源于循环血液（cPGCs）和性腺（gPGCs）的鸡原始生殖细胞可以与MSCs饲养层细胞一起维持和增殖。在MSCs饲养基上共培养的PGCs保留了其多能性，表达PGCs特异性基因和干性标记，并保持未分化状态。我们的研究表明，无异种MSC饲养系统是PGCs生长和扩增的良好候选者，是转基因鸡研究的垫脚石。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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干细胞探索(英文)

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