精准医学研究最新文献

{"title":"Investigating the effect of rubiadin cytotoxicity and expression of BAX and BCL2 genes on HepG2 liver cancer cell line","authors":"Sanaz Pashapour, Abbas Zabihi, Y. Hamidi, M. Heshmati","doi":"10.53388/pmr20230014","DOIUrl":"https://doi.org/10.53388/pmr20230014","url":null,"abstract":"Background: Rubiadin is a type of anthraquinone compound that can be found in Rubiaceae plants, such as Ronas. Nonetheless, only limited research has been done to explore the potential anticancer properties of rubiadin on liver cancer cells. Thus, the objective of the present study is to examine how rubiadin affects the viability of liver cancer cells as well as normal cells. Methods: HepG2 and AGO cell lines were assigned into controls (not exposed to rubiadin) and groups with exposure to rubiadin with 12.5, 6.25, 3.125, 1.56, 0.78, and 0.39 μg/mL concentrations. 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide and reverse transcription-polymerase chain reaction were used to measure cell viability, and one-way analysis of variance was used for data analysis. Results: The viability of liver cancer cells was significantly reduced when exposed to 12.5, 6.25, 3.125, and 1.56 μg/mL concentrations ( P < 0.01). An IC 50 of 44.73 μg/mL was reported. Furthermore, the BAX gene’s relative expression ( P < 0.05) was significantly increased and the BCL2 gene expression ( P < 0.05) was significantly reduced. The average ratio of BAX gene expression to BCL2 increased significantly ( P < 0.01). Conclusion: This research showed that rubiadin decreases cell viability by increasing the ratio of BAX gene expression to BCL2. In addition rubiadin has no cytotoxic effect on normal cells.","PeriodicalId":59651,"journal":{"name":"精准医学研究","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70817235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances on biological evaluation methods of programmed cell death protein-1/ligand-1 inhibitors","authors":"Qi Miao, Wanwan Zhang","doi":"10.53388/pmr20230011","DOIUrl":"https://doi.org/10.53388/pmr20230011","url":null,"abstract":"Immuno-oncology represents a groundbreaking and well-established field within cancer treatment. Among the various immuno-oncology targets, the exploration of programmed cell death-1/ligand-1 for drug discovery has proven to be one of the most successful endeavors. Remarkably, it took nearly 30 years from the initial target identification to the clinical approval of monoclonal antibodies. Providing suitable and reliable bioassays for drug candidate evaluation is of paramount importance throughout the early stages of drug discovery, from lead compound identification to in vivo efficacy testing. This assay review aims to shed light on diverse assays reported in the literature for testing antagonism activity and efficacy of programmed cell death-1/ligand-1 inhibitors. Each of these assays possesses inherent advantages and can be applied in different research scenarios. The insights presented in this summary can serve as a valuable resource for scientists in this field, aiding in the selection of appropriate assays for their specific investigations.","PeriodicalId":59651,"journal":{"name":"精准医学研究","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70816743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pan-cancer analysis of positive regulatory domain-containing 16 in human tumors","authors":"Shining Zhang, Tingpeng Yang, Xiang He, Fang Yang, Lin Zhang","doi":"10.53388/pmr20230012","DOIUrl":"https://doi.org/10.53388/pmr20230012","url":null,"abstract":"Background: Positive regulatory domain-containing 16 (PRDM16) plays a key role in brown adipose transcription, but its function in cancer is unclear. Our research to investigate the potential roles of PRDM16 across multiple types of cancer by pan cancer analyses. Methods: UALCAN and TIMER2 database were utilized to evaluate PRDM16 expression in cancer patients. Gene Expression Profiling Interactive Analysis was employed to analyze the overall survival and disease-free survival across all The Cancer Genome Atlas Program tumors. Using the cBioPortal tool, we analyzed the mutation features of PRDM16 for the The Cancer Genome Atlas Program tumors, then utilized the Encyclopedia of RNA Interactomes database to predict the miRNA-mRNA relationships associated with the PRDM16 for all tumors. Results: The expression level of PRDM16 in the tumor tissues is lower than that in the normal tissues. Interesting, the high expression of PRDM16 has a positive effect on the prognosis of kidney clear cell carcinoma and lung adenocarcinoma, but not conducive to the prognosis of most cancers. In multiple cancer types, the expression of PRDM16 was significantly positively correlated with immune infiltration of cancer-associated fibroblasts. Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analysis indicated that PRDM16 may be related to transcriptional misregulation pathway in cancer. We identified potential miRNAs that play regulatory roles of PRDM16 in kidney clear cell carcinoma and lung adenocarcinoma. Conclusion: PRDM16 is expressed in different cancers, it can be used as a biomarker for prognosis of pan-cancer and is associated with immune infiltration.","PeriodicalId":59651,"journal":{"name":"精准医学研究","volume":"21 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70816758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of estrogen-mediated ubiquitin on cardiovascular diseases: a bioinformatics analysis","authors":"Nan Li, Yu-Han Duan, Kun Zhang","doi":"10.53388/pmr20230002","DOIUrl":"https://doi.org/10.53388/pmr20230002","url":null,"abstract":"","PeriodicalId":59651,"journal":{"name":"精准医学研究","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70816802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suppressed effects of Phyllanthus urinaria L. ethyl acetate extract on hepatitis B virus both in vitro and in vivo","authors":"Zhilong Chen, Rui Zhang","doi":"10.53388/pmr20230015","DOIUrl":"https://doi.org/10.53388/pmr20230015","url":null,"abstract":"Background: Phyllanthus urinaria L. ( P. urinaria ) extract (PUE) has been used to inhibit hepatitis B virus (HBV). However, the underlying mechanism remains unclear. To investigate which PUE fractions and main components lead to against HBV and approach the relevant molecular mechanisms. Methods: P. urinaria was extracted with water, and then the decoction was extracted by petroleum ether, ethyl acetate, and n-butanol in turn. The HepG2.2.15 cell was treated with aqueous fraction, petroleum ether fraction, ethyl acetate fraction and n-butanol fraction, gallic acid (GA, C 7 H 6 O 5 ) and corilagin (CL, C 27 H 22 O 18 ), respectively. The medium was collected for hepatitis B surface antigen (HBsAg) and hepatitis B e antigen assays. Cell counting kit-8 method was used to identify cell proliferation. Also, the levels of cellular oxygen consumption, reactive oxygen species, and reduced glutathione were detected. The HBV modeling mice were treated with ethyl acetate fraction, entecavir and physiological saline, respectively. The serum was collected for HBsAg and inflammatory cytokines assays. Liver tissue metabolites were screened by LC-MS/MS method. Results: The ethyl acetate fraction (EAF) of P. urinaria could significantly inhibit HBV secretion in HepG2.2.15 ( P < 0.05). Furthermore, two main constitutes in ethyl acetate fraction, GA and CL, could significantly inhibit HBV secretion and reduced cell proliferation ( P < 0.05). Also, GA and CL could increase cellular oxygen consumption, intracellular superoxide anions level, superoxide dismutase level and glutathione depletion. Compared with the Modeling group, EAF significantly decreased the expression levels of HBsAg, IL-1β, IFN-α ( P < 0.05). LC-MS/MS analysis results showed that EAF dramatically up-regulate hydroxyproline, maltotriose, betaine and down-regulate glutathione disulfide, taurocholate, taurochenodeoxycholate ( P < 0.05). Kyoto Encyclopedia of Genes and Genomes results show that the differential metabolites were mainly enriched in ATP-binding cassette transporters pathway. Conclusions: P. urinaria exhibits suppressed effects on HBV by modulating reactive oxygen species formation or metabolomics both in vitro and in vivo. These data indicate that P. urinaria may be an alternative therapeutic agent for the treatment of HBV-related hepatitis.","PeriodicalId":59651,"journal":{"name":"精准医学研究","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70817287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recognition of prognostic biomarker and its association with immune infiltrates in breast cancer associated with inflammation","authors":"Shunjin Wang, Zhi Li, Huan Zhang, Qun Wang","doi":"10.53388/pmr20220001","DOIUrl":"https://doi.org/10.53388/pmr20220001","url":null,"abstract":"","PeriodicalId":59651,"journal":{"name":"精准医学研究","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70815969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic implication and oncogenic role of RHCG in lung adenocarcinoma","authors":"Yidan Sun, Xiaojiang Li, Peiying Yang, Minna Huang, Yingjie Jia","doi":"10.53388/pmr20220013","DOIUrl":"https://doi.org/10.53388/pmr20220013","url":null,"abstract":"","PeriodicalId":59651,"journal":{"name":"精准医学研究","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70816421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of bisphenol A action on genes interfering with estrogen signaling pathways and the development of endometriosis","authors":"Nan Li, Yuhan Duan, Lei Chen, Kun Zhang","doi":"10.53388/pmr20220012","DOIUrl":"https://doi.org/10.53388/pmr20220012","url":null,"abstract":"","PeriodicalId":59651,"journal":{"name":"精准医学研究","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70816785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Mig6 gene in tumor research","authors":"YeMinXiao Zeng, An Yan, Zhonggang Wu, Wenjie Zeng, Huaiyuan Zheng, Xiuling Wei, Shibo Zhao, Zihui Liu","doi":"10.53388/pmr20220005","DOIUrl":"https://doi.org/10.53388/pmr20220005","url":null,"abstract":"","PeriodicalId":59651,"journal":{"name":"精准医学研究","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70816107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical research progress of first-line immunotherapy for extensive-stage small cell lung cancer","authors":"Yongchao Yu, Hongxia Xie, Jinhui Zuo, Xiaojiang Li, Yingjie Jia, F. Kong","doi":"10.53388/pmr20220004","DOIUrl":"https://doi.org/10.53388/pmr20220004","url":null,"abstract":"","PeriodicalId":59651,"journal":{"name":"精准医学研究","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70816062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}