微生物学(英文)最新文献_第3页

Identification of Pathogen of Skin Rot of Rana spinosa and Study on Immune Effect of Vaccine 棘蛙皮腐病病原菌鉴定及疫苗免疫效果研究

微生物学(英文) Pub Date : 2023-01-01 DOI: 10.12677/amb.2023.121005

欢苏

{"title":"Identification of Pathogen of Skin Rot of Rana spinosa and Study on Immune Effect of Vaccine","authors":"欢苏","doi":"10.12677/amb.2023.121005","DOIUrl":"https://doi.org/10.12677/amb.2023.121005","url":null,"abstract":"In order to determine the pathogen of the skin rot disease of Rana spinosa and provide theoretical basis for the prevention and control of the disease in production, this study isolated the pathogenic bacteria from the diseased frog focus, carried out artificial infection and the morphological characteristics, physical and chemical characteristics and molecular biological analysis of the pathogenic bacteria, and carried out drug sensitivity test and vaccine immunity and control test. The results showed that the dominant bacteria QS01 isolated from the diseased lesions of Rana spino-sa was confirmed to be the pathogen of skin rot disease of Rana spinosa by artificial regression infection test. Combined with its phenotypic characteristics and 16S rRNA, gyrB gene sequence analysis, it was determined to be Citrobacter freundii . The medicine sensitive test result showed that the bacteria is sensitive to eight kinds of antibiotics including Enrofloxacin, Chloramphenicol, Ceftazi-dimeetc, but lowly sensitive or resistant to other tested antimicrobial agents. Adopting these ways included injection, immersion and atomizing to inoculate frog with vaccine named QS01 inactivated by formaldehyde, average titer of three immunity groups reached the peak in the 20 d. The highest serum antibody titers were 1:64~128 (101.6), 1:16~32 (20.2) and 1:16~32 (16), which were significantly higher than those of the control group. The immune protection rate for Q. spi-nosa after injection, immersion and spray of QS01 inactivated vaccine can reach 100%, 85.71% and 71.43%. Our research shows that Citrobacter frederii is the pathogen of skin rot disease of Rana spinosa , and it is effective and feasible to use vaccine to control ulcerative skin disease.","PeriodicalId":56408,"journal":{"name":"微生物学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66129056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Post-Harvest Fungi Associated with Cowpea (Vigna unguiculata L. Walp.) Seeds Produced in Burkina Faso 与豇豆相关的收获后真菌(Vigna unguiculata L. Walp.)种子产自布基纳法索

微生物学(英文) Pub Date : 2023-01-01 DOI: 10.4236/aim.2023.133009

Amidou S. Ouili, Y. Maiga, M. Nikiéma, Souleymane Bissiri, Y. Dabiré, Illiassou Mogmenga, Cheik Omar Tidiane Compaoré, A. Ouattara

引用次数: 0

Microbiological Quality of Freshly Prepared, Packaged Fruit and Milk Juices Sold in Cafés, Shops, and Supermarkets in Hargeisa, Somaliland 索马里兰哈尔格萨咖啡馆、商店和超市销售的新鲜制备、包装水果和牛奶果汁的微生物质量

微生物学(英文) Pub Date : 2023-01-01 DOI: 10.4236/aim.2023.135013

Mohamud Ahmed Farah, A. Esa, Helmi S. Abdelaziz

引用次数: 0

Potential Risk of Transmission of Escherichia coli and Salmonella spp. Infections by the Musca domestica Fly and the Periplaneta americana Cockroach in the City of Cotonou (South Benin) 南贝宁科托努市家蝇和美洲大蠊传播大肠杆菌和沙门氏菌感染的潜在风险

微生物学(英文) Pub Date : 2023-01-01 DOI: 10.4236/aim.2023.139029

Tatcheme Filemon TatTokponnon, Bidossessi Brunelle Ella Agassounon, Daton Sylvain Kougblenou, R. Ossè, V. Dougnon, Calmette Nouwagbe Daga, Justin Kossou, Idayath Joachelle Gounou Yerima, Festus Houessinon, Sare Dabou Zoulkifilou, Martin Akogbeto

引用次数: 0

Amplification of Hidden Fluorescent DNA 隐藏荧光DNA的扩增

微生物学(英文) Pub Date : 2023-01-01 DOI: 10.12677/amb.2023.121006

自厚刘

引用次数: 0

Electron Micrographic Representations of Mechanisms of Action of Murine Norovirus on ATCC TIB-71 Cells and Level of Gene Expression 诺如病毒对ATCC TIB-71细胞作用机制及基因表达水平的电镜观察

微生物学(英文) Pub Date : 2023-01-01 DOI: 10.4236/aim.2023.131003

Uchenna B. Iloghalu, S. Miller, A. Ewunkem, J. Khatiwada, S. Davis, L. Williams

引用次数: 0

Escherichia coli Pathotypes in Children with Acute Diarrhea in an Informal Settlement in Nairobi, Kenya 在肯尼亚内罗毕的一个非正式定居点，急性腹泻儿童的大肠杆菌致病性

微生物学(英文) Pub Date : 2023-01-01 DOI: 10.4236/aim.2023.134011

Moureen Jepleting, W. Mutai, V. Musyoki, Beatrice Oduor, C. Ayodo, R. Mugoh, S. Kariuki, S. Omulo

引用次数: 0

Expression Level of Inflammation-Related Cytokines in the Progression of Chronic Hepatitis B and Their Correlation with Clinical Indexes 慢性乙型肝炎进展过程中炎症相关细胞因子的表达水平及其与临床指标的相关性

微生物学(英文) Pub Date : 2023-01-01 DOI: 10.12677/amb.2023.121003

洁陈

{"title":"Expression Level of Inflammation-Related Cytokines in the Progression of Chronic Hepatitis B and Their Correlation with Clinical Indexes","authors":"洁陈","doi":"10.12677/amb.2023.121003","DOIUrl":"https://doi.org/10.12677/amb.2023.121003","url":null,"abstract":"Objective: To detect the expression levels of cytokines secreted by immune cells in patients with chronic hepatitis B (CHB), liver cirrhosis (LC) and hepatocellular carcinoma (HCC), and to investigate their correlation with clinical indicators, and in order to provide new for clinical diagnosis and treatment. Methods: 106 cases of CHB, 96 cases of LC, 21 cases of HCC from March 1, 2020 to May 31, 2020, in the third people’s Hospital of Kunming. The changes of liver function indexes such as ALT, AST, TBIL, IBIL and DBIL were detected by automatic biochemical analyzer. The expression of cytokines IL-1 β , IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12P70, IL-17, TNF-α , IFN-γ and IFN-α in peripheral blood was detected by multiple microsphere flow immunofluorescence assay. And the correlation between the above liver function indexes and cytokines was analyzed. As comparison, 100 healthy people served as normal control group. Results: The expressions of AST, ALT, TBIL, DBIL and IBIL in CHB, LC and HCC groups were higher than normal control group. Excepted IL-5, IFN-α and IL-6, the expression of IL-2, IL-1 β , IFN-γ , IL-8, IL-17, IL-12P70 and TNF-α in CHB, LC and HCC group was significantly higher than normal control group ( P < 0.05), while the expression of IL-4 and IL-10 was significantly lower than normal control group ( P < 0.05). There were significant differences in TNF-α and IL-4 among CHB, LC and HCC groups ( P < 0.05). Cytokines IL-17 and TNF-α were significantly correlated with AST, ALT, TBIL, DBIL and IBIL ( P < 0.05). Conclusion: The secretion of pro-inflammatory cytokines (IL-1 β , IL-2, IL-5, IL-6, IL-8, IL-12P70, IL-17, TNF-α , IFN-γ , IFN-α ) increased, while the secretion of anti-inflammatory cytokines (IL-4, IL-10) decreased in CHB, LC and HCC. The liver damage caused by inflammatory immune reaction was increase led to the deterioration of the disease","PeriodicalId":56408,"journal":{"name":"微生物学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66128993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cascade Signal Amplification Rapid Detection of Two Pathogenic Bacteria in Dairy Products Using Magnetic Bead Nanoprobes Combined with Immunochromatographic 磁珠纳米探针结合免疫层析的级联信号扩增快速检测乳制品中两种致病菌

微生物学(英文) Pub Date : 2023-01-01 DOI: 10.12677/amb.2023.122011

菲卢

引用次数: 0

Opportunities and Challenges of Application of the Probiotics/Postbiotics in Cosmetics and Female Personal Care Products 化妆品及女性个人护理产品中益生菌/后益生菌应用的机遇与挑战

微生物学(英文) Pub Date : 2023-01-01 DOI: 10.12677/amb.2023.121001

冠楠李

引用次数: 0