Food and Bioprocess Technology最新文献

{"title":"Nanoporous Carriers for Active, Sustainable Food-Packaging Films and Coatings: A Cross-Platform Comparison and Design Rules","authors":"Mattia Fornaro,&nbsp;Barbara Liguori,&nbsp;Domenico Caputo","doi":"10.1007/s11947-026-04605-6","DOIUrl":"10.1007/s11947-026-04605-6","url":null,"abstract":"<div><p>Active and sustainable food packaging increasingly relies on additives (antioxidants, antimicrobials, and gas or volatile scavengers) that extend shelf life and reduce food waste, yet delivering these functions from thin films and coatings without sacrificing processability, transparency, or food-contact safety remains a persistent engineering problem, and effectiveness is still commonly pursued by raising additive dosage. Nanoporous solids offer an alternative, because their tunable pores and high internal surface area allow actives to be stored on internal surfaces and metered through pore architecture and surface chemistry, decoupling function from dosage, while the same fillers can improve barrier performance through tortuosity and sorption. Because the literature has developed platform by platform, this review compares five families (zeolites, ordered mesoporous silica, halloysite nanotubes, metal–organic frameworks, and porous carbons) as additive carriers for flexible food-packaging films and coatings, linking pore architecture and surface chemistry to loading, release, scavenging, and barrier enhancement, and to scalable integration routes including melt compounding, solvent casting, and waterborne coating. Release-kinetic analysis and an order-of-magnitude ethylene mass balance indicate that affinity and access under realistic humidity, rather than nominal capacity, usually limit performance, and comparative benchmarking shows that no single platform dominates: zeolites and porous carbons rate highly on cost and processing robustness, metal–organic frameworks on loading and selectivity, and mesoporous silica and halloysite occupy a deployable middle ground. Future work should prioritize quantitative pore-transport design rules, one-filler multi-function systems, manufacturing-ready waterborne topcoats, and standardized migration testing tailored to nanoporous carriers.</p></div>","PeriodicalId":562,"journal":{"name":"Food and Bioprocess Technology","volume":"19 10","pages":""},"PeriodicalIF":5.6,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11947-026-04605-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149132062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Glutamic Acid Esterification on the Microstructure and Physicochemical Properties of Rice Husk-Derived Cellulose Nanocrystals","authors":"Jiaying Yang,&nbsp;JiaYu Zheng,&nbsp;Li Niu,&nbsp;Yayun Hu,&nbsp;Wenhao Li","doi":"10.1007/s11947-026-04595-5","DOIUrl":"10.1007/s11947-026-04595-5","url":null,"abstract":"<div><p>Rice husk, an abundant agricultural waste with limited high-value applications, can be valorized by converting its cellulose into functional nanomaterials. In this study, rice husk-derived cellulose nanocrystals (CNCs), denoted as RNC in the sample nomenclature, were prepared and subsequently modified via esterification using different concentrations of L-glutamic acid hydrochloride (0.1, 0.3, 0.6, 0.9, and 1.2 mol/L) to produce glutamic-acid-modified RNC (GRNC). The treatment increased the nitrogen content and degree of substitution (DS), supporting the successful introduction of glutamic acid moieties. The modified CNCs largely retained the cellulose I crystalline structure, while their crystalline characteristics changed after esterification. Microstructural analysis showed that the characteristic nanoscale morphology of the CNCs was largely retained after modification. Zeta potential analysis indicated enhanced electrostatic stability after modification, with the sample modified at 0.6 mol/L showing the highest absolute zeta potential. Glutamic acid esterification also altered the thermal behavior of the CNCs, with an overall improvement in thermal stability. Correlation analysis further indicated associations among the extent of modification, crystalline characteristics, surface charge, and thermal properties, supporting a modification degree–structure–property relationship and providing a basis for the potential application of the modified CNCs in bio-based packaging materials.</p></div>","PeriodicalId":562,"journal":{"name":"Food and Bioprocess Technology","volume":"19 10","pages":""},"PeriodicalIF":5.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149132321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green Synthesis of Multifunctional Ag/ZnO Nanoparticles Using Vitis labrusca L. Extract: Bioactivity, Malachite Green Adsorption, and HMF Removal","authors":"Rama Hammoudeh,&nbsp;Pınar Bozbeyoğlu,&nbsp;Ömer Karpuz,&nbsp;Abdulkadir Gül,&nbsp;Nebahat Ejder,&nbsp;Cemalettin Baltacı","doi":"10.1007/s11947-026-04594-6","DOIUrl":"10.1007/s11947-026-04594-6","url":null,"abstract":"<div><p>In this study, Ag/ZnO nanoparticles (Ag/ZnO NPs) were synthesized via a green route using <i>Vitis labrusca</i> L. extract and evaluated for their bioactive, environmental performance. BM-1 and BM-2 were prepared under different precursor/extract ratios and pH conditions to assess the influence of synthesis parameters. UV–Vis, FT-IR, XRD, HRTEM, DSC, and EDX analyses indicated the successful formation of Ag/ZnO nanostructures with distinct physicochemical features. HRTEM analysis determined that BM-1 exhibited an average particle diameter of 23.18 ± 11.84 nm, while BM-2 demonstrated a mean size of 6.14 ± 1.30 nm. The corresponding relative size-dispersion values were 0.261 for BM-1 and 0.045 for BM-2, indicating a more uniform particle size distribution for BM-2. Antioxidant assays showed that BM-2 exhibited the strongest overall antioxidant performance in DPPH, ABTS•⁺, Fe³⁺, and Cu²⁺ assays. Both NPs exhibited antibacterial activity against Gram-positive and Gram-negative bacteria and showed stronger effects than the grape extract alone. Cytotoxicity assays revealed dose-dependent inhibition in A549 and HeLa cancer cells, while lower toxicity was observed in normal ARPE-19 cells, suggesting cell-type-dependent biological response. In adsorption experiments, the NPs effectively removed malachite green, with isotherm analysis providing stronger support for the Langmuir model for BM-1 and comparable Langmuir and Freundlich fits for BM-2, while the pseudo-second-order model provided the best description of the adsorption kinetics. The maximum dye adsorption capacities were 85.5 mg/g for BM-1 and 101.3 mg/g for BM-2. Under optimum conditions (5.0 g/L NPs, 240 min), the highest HMF removal efficiencies reached 44.49% for BM-2 in standard aqueous solutions, whereas competitive matrix interactions in apple juice limited the maximum efficiency to 29.27%. Overall, this study highlights the value of integrating green synthesis, bioactivity, dye adsorption, and food-related contaminant removal within a single Ag/ZnO nanoplatform.</p></div>","PeriodicalId":562,"journal":{"name":"Food and Bioprocess Technology","volume":"19 10","pages":""},"PeriodicalIF":5.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149132330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-destructive Multi-attribute Prediction of Beef Freshness Using Excitation–Emission Matrix Fluorescence Spectroscopy and a Dual-Channel Multi-task Network","authors":"Mingze Li,&nbsp;Ruoxin Chen,&nbsp;Wei Ning,&nbsp;Xufen Xie,&nbsp;Jingran Bi,&nbsp;Gongliang Zhang,&nbsp;Hongman Hou","doi":"10.1007/s11947-026-04598-2","DOIUrl":"10.1007/s11947-026-04598-2","url":null,"abstract":"<div><p>Rapid, non-destructive assessment of beef freshness is important for cold-chain distribution, quality grading, and safety control. Conventional physicochemical methods are time-consuming, destructive, and poorly suited to simultaneous multi-index evaluation. This study developed a total volatile basic nitrogen (TVB-N)-guided Asymmetric Cascade excitation–emission matrix (EEM) Network (TAC-EEMNet) for simultaneous prediction of TVB-N, pH, and CIELAB redness (<i>a</i>^*) from EEM fluorescence spectra. Two complementary inputs were constructed: an intensity-preserved channel representing fluorescence-magnitude variation and a spectral-shape-enhanced channel emphasizing normalized peak structures. Independent convolutional neural network (CNN) branches with squeeze-and-excitation (SE) attention extracted features from the two channels and fused them with storage-time information. TVB-N was treated as the primary freshness task, and its gradient-detached intermediate representation guided the auxiliary pH and <i>a</i>^* branches. Across repeated runs, TAC-EEMNet achieved mean test-set <i>R</i>² values of 0.9200 for TVB-N and 0.8109 for pH while remaining competitive for <i>a</i>^*. Relative to partial least squares regression (PLSR), random forest regression (RF), support vector regression (SVR), MLP-dual, and CNN-dual, the model showed superior performance for the core freshness indicator and weighted overall evaluation. Fusion Signed Grad-CAM and region-of-interest (ROI) occlusion further showed that prediction relied mainly on the principal EEM peak region at approximately <i>E</i>_x 250–320 nm and <i>E</i>_m 300–400 nm, with substantial contributions from peak-shape-related regions in the spectral-shape-enhanced channel. TAC-EEMNet therefore provides an interpretable framework that integrates EEM intensity, spectral shape, and storage time for non-destructive, multi-attribute evaluation of beef freshness under controlled conditions in which storage history is known or traceable.</p></div>","PeriodicalId":562,"journal":{"name":"Food and Bioprocess Technology","volume":"19 10","pages":""},"PeriodicalIF":5.6,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149133126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of Oil-in-Water Emulsions Stabilized by Soy Protein and Lecithin Using Premix Microchannel Emulsification","authors":"Jinhyun Park,&nbsp;Takumi Umeda,&nbsp;Tetsuya Araki,&nbsp;Isao Kobayashi","doi":"10.1007/s11947-026-04599-1","DOIUrl":"10.1007/s11947-026-04599-1","url":null,"abstract":"<div><p>This study characterized oil-in-water (O/W) emulsions produced by premix microchannel emulsification (PMCE) using soybean-derived emulsifiers: soy protein isolate (SPI), modified lecithin (ML), and soy lecithin (SL). Mean droplet diameter (<i>d</i>₄,₃) and relative span factor (RSF) were evaluated under varying emulsifier concentrations, dispersed phase fractions, operating pressures, and pass numbers, whereas flux and storage stability were examined for selected representative systems. Among the hydrophilic emulsifier concentrations tested, only SPI significantly affected both <i>d</i>₄,₃ and RSF. Increasing SPI concentration from 0.2 to 0.6% increased <i>d</i>₄,₃ from 9.60 to 12.83 µm, while reducing RSF from 2.45 to 1.55, indicating the formation of larger but more uniform droplets. SL addition induced nonlinear responses in the SPI and ML systems. In the ML system, increasing the initial dispersed phase fraction in the premix from 5 to 25% increased <i>d</i>₄,₃ from 3.69 to 14.27 µm and decreased RSF from 8.43 to 2.31, suggesting a shift in breakup behavior. The <i>d</i>₄,₃ value increased with operating pressure in all systems, whereas a second pass did not significantly affect either <i>d</i>₄,₃ or RSF in any group. Owing to its interfacial activity, low interfacial tension (7.23 mN/m), and low bulk viscosity (1.54 mPa·s), 0.3% ML showed the highest flux, reaching 60.4–77.2% of that of Tween 80 at 5–15 kPa. Overall, 0.6% SPI provided the best balance of droplet uniformity and storage stability. These findings advance the mechanistic understanding of emulsion behavior under PMCE and guide the optimization of low-energy emulsification processes suitable for shear-sensitive or heat-labile formulations.</p></div>","PeriodicalId":562,"journal":{"name":"Food and Bioprocess Technology","volume":"19 10","pages":""},"PeriodicalIF":5.6,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149132971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Data-Centric Machine Learning for Avocado Firmness Prediction using Smartphone Imaging: Effects of Training-Set Size, Image Resolution, and Multi-View Inference","authors":"In-Hwan Lee,&nbsp;Dhimant Morakhia,&nbsp;Xinzhou Ge,&nbsp;Luyao Ma","doi":"10.1007/s11947-026-04600-x","DOIUrl":"10.1007/s11947-026-04600-x","url":null,"abstract":"<div><p>Machine learning coupled with computer vision enables rapid, non-destructive food quality assessment. However, most studies focus on maximizing prediction accuracy, while quantitative criteria for data requirements remain underdeveloped. Because ground-truth quality labels often require labor-intensive and destructive measurements, determining sufficient data is important. This study developed a statistics-informed framework to evaluate training-set size, image resolution, and multi-view inference, using avocado firmness prediction as a case study. ResNet18-based convolutional neural network (CNN), random forest (RF), and support vector regression (SVR) were trained on 100–1100 smartphone images and evaluated with fixed validation (n = 150) and test (n = 150) sets. Twenty independent runs were analyzed using blocked analysis of variance to consider run-to-run variation in sample selection and model training. For CNN, 500 images were the smallest set for which test R² did not differ significantly from the largest set in this study (R² = 0.796 ± 0.080 <i>versus</i> 0.849 ± 0.039; <i>P</i> &gt; 0.05; 95% CI for the difference: -0.114 to 0.009), compared with 700 images for RF and 500 images for SVR. Reducing image resolution from 224 × 224 to 56 × 56 pixels did not significantly reduce CNN accuracy. Combining smaller training sets with lower resolution reduced training time by up to 87.314 ± 0.214%. The number of inference views per fruit affected fruit-level RMSE, with 7 views achieving a significantly lower RMSE than 3 views in adjusted pairwise comparisons (<i>P</i> = 0.015). This framework provides statistics-based guidance for designing machine learning workflows that balance predictive performance with data collection and computational costs.</p></div>","PeriodicalId":562,"journal":{"name":"Food and Bioprocess Technology","volume":"19 10","pages":""},"PeriodicalIF":5.6,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149132970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"H2 MAP Extends the Shelf Life of Juicy Peach by Delaying Softening and Maintaining Antioxidant Capacity","authors":"Guanjie Zhu,&nbsp;Shanshan Jin,&nbsp;Huanhuan Shan,&nbsp;Xin Zhou,&nbsp;Feng Cao,&nbsp;Changxi Li,&nbsp;Wenbiao Shen","doi":"10.1007/s11947-026-04596-4","DOIUrl":"10.1007/s11947-026-04596-4","url":null,"abstract":"<div><p>Hydrogen-modified atmosphere packaging (H₂ MAP) is effective for fruit and vegetable preservation, yet relatively few studies have focused on softening-susceptible fruits. ‘Hujing’ juicy peaches are susceptible to browning and softening during storage, thereby limiting storage quality. To address this problem, an air control and three H₂ MAP treatments containing 0.01%, 0.1%, and 1% H₂ were applied. Among the treatments, 0.1% H₂ MAP showed the strongest overall preservation effect during 32 d of cold storage (4 ± 1 °C and 85% relative humidity), with a smaller total color difference (Δ<i>E</i>), decreased polyphenol oxidase (PPO) activity, and better maintenance of total phenolic content. The decreases in total soluble solids content, soluble sugar content, and firmness were attenuated, while weight loss was reduced. Higher protopectin and cellulose contents and lower water-soluble pectin levels were maintained, accompanied by lower polygalacturonase (PG) and pectin methylesterase (PME) activities during the middle and late stages of storage. Transcript analysis further indicated that storage-induced increases in the expression of genes related to pectin degradation (<i>PpPG1/21/22</i>, <i>PpPME1</i>, and <i>PpPL1/PpPL2</i>) and cellulose degradation (<i>PpCel20</i> and <i>PpEG1</i>) were generally slowed down. In addition, 0.1% H₂ MAP better maintained antioxidant capacity and limited oxidative damage during storage. Collectively, these results indicate that 0.1% H₂ MAP improved the cold-storage quality of ‘Hujing’ juicy peaches.</p></div>","PeriodicalId":562,"journal":{"name":"Food and Bioprocess Technology","volume":"19 10","pages":""},"PeriodicalIF":5.6,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149035067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine Learning-Enabled FT-IR Analysis of an Enzyme-Based Surrogate for Rapid Sanitation Verification","authors":"Hemiao Cui,&nbsp;Luyao Ma,&nbsp;Reza Ovissipour,&nbsp;Nitin Nitin","doi":"10.1007/s11947-026-04591-9","DOIUrl":"10.1007/s11947-026-04591-9","url":null,"abstract":"<div><p>Sanitizer washing is a critical intervention for controlling cross-contamination in fresh produce processing. Rapid verification of sanitizer efficacy is therefore essential, but current approaches are limited in their ability to assess both antimicrobial efficacy and sanitizer concentration in real time. Using bacterial surrogates to verify antimicrobial efficacy requires cultivation, which can take at least 1 day. To resolve this challenge, this study developed a bacteria-free biomarker strategy using catalase, a stress-responsive bacterial enzyme, as an enzyme-based surrogate for sanitation verification. Catalase was treated with sodium hypochlorite (NaOCl), and the resulting structural and activity changes were characterized using Fourier-transform infrared (FT-IR) spectroscopy and machine learning. NaOCl treatment caused distinct changes in the spectra of catalase, with spectral shifts in the amide I and II regions indicating protein denaturation and aggregation. Five-fold cross-validation on 105 FT-IR spectral samples was used to validate the predictive models. Partial least squares regression accurately predicted NaOCl concentration from catalase spectra, with a coefficient of determination (<i>R</i>²) of 0.98. A LightGBM gradient-boosting model predicted whether bacterial reduction exceeded 2 log CFU/mL from catalase spectra, achieving an area under the curve of 0.91. These findings demonstrate the potential of catalase as a non-living, cell-free surrogate indicator for bacterial inactivation and rapid sanitization verification in fresh produce wash systems.</p></div>","PeriodicalId":562,"journal":{"name":"Food and Bioprocess Technology","volume":"19 10","pages":""},"PeriodicalIF":5.6,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149035453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solution-mediated Reconstruction of Electrospun Poly (vinyl alcohol)/Chitosan Fibrous Membranes into Transparent Antimicrobial Films with Immobilized Polyionene for Active Packaging","authors":"Xinmei Xue,&nbsp;Zhengkai Wang,&nbsp;Liying Lan,&nbsp;Jiameng Shi,&nbsp;Qiaohua Qiu","doi":"10.1007/s11947-026-04593-7","DOIUrl":"10.1007/s11947-026-04593-7","url":null,"abstract":"<div><p>Transparent active packaging can facilitate visual quality assessment while extending the shelf life of fresh produce. However, conventional transparent films generally lack antimicrobial activity, whereas the incorporation of active agents often impairs optical or mechanical properties. In this study, transparent antimicrobial films were prepared by aqueous solution-mediated reconstruction of electrospun poly (vinyl alcohol)/chitosan (PVA/CS) fibrous membranes, accompanied by glutaraldehyde (GA)-mediated chemical stabilization. Immersion in aqueous GA caused fiber swelling and partial dissolution, whereas subsequent drying promoted macromolecular rearrangement, fiber coalescence, and collapse of interfibrillar voids. Concurrent GA crosslinking stabilized the reconstructed continuous matrix. The optimized GA-PVA/CS film exhibited a transmittance of 90.2% at 600 nm, a tensile strength of 32.4 MPa, and an elongation at break of 80.9%. Silane-functionalized polyionene was incorporated into the electrospinning precursor, and silanol condensation and self-condensation promoted its retention within the reconstructed matrix. The resulting films retained visible-light transmittance of 87.5–90.4%, enhanced UV attenuation, and reduced water vapor permeability. At polyionene loadings of 3% and 5%, the films produced near-complete inhibition of <i>Escherichia coli (E. coli)</i> and <i>Staphylococcus aureus (S. aureus)</i> and delayed the radial growth of <i>Botrytis cinerea</i> (<i>B. cinerea</i>). Extracts of the final films maintained at least 89% relative metabolic activity in L929 fibroblasts, while the maximum mean GA migration was 0.105 mg/dm² under the tested conditions. A descriptive blueberry-packaging experiment using one independent packaging unit per treatment suggested reductions in visible deterioration, individual-fruit weight loss, packaging-level decay, and changes in juice pH. These results provide a route for converting electrospun PVA/CS fibrous membranes into transparent, flexible, and antimicrobial films for active packaging.</p></div>","PeriodicalId":562,"journal":{"name":"Food and Bioprocess Technology","volume":"19 10","pages":""},"PeriodicalIF":5.6,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149034964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction of Echinophora platyloba Polysaccharides (EPPs) by Natural Deep Eutectic Solvents Coupled with Ultrasound and Enzyme and Ultrasound-Assisted Interaction of EPPs/Oak Protein Isolate as a Potential Prebiotic","authors":"Razieh Hemati-Farsani,&nbsp;Sabihe Soleimanian-Zad,&nbsp;Shokouh Ahmadi","doi":"10.1007/s11947-026-04560-2","DOIUrl":"10.1007/s11947-026-04560-2","url":null,"abstract":"<div><p><i>Echinophora platyloba</i> polysaccharides (EPPs) were initially extracted utilizing natural deep eutectic solvents (NADESs) coupled with ultrasound and enzymatic methods. Subsequently, the EPPs were reacted with oak protein isolate (OPI) at varying ultrasound power (225, 450, and 675 W). The optimal NADES (ChCl-LA) for extracting EPPs, when combined with ultrasound (150 W) and cellulase enzyme, achieved an extraction efficiency of 71.33 ± 1.42%. The molecular weight of the EPPs was determined to be 8032 kDa, comprising 45.94% glucose, 18.68% glucuronic acid, and 32.9% arabinose. The EPPs’ anomeric configurations were validated through FTIR and NMR analyses. An increase in ultrasound intensity from 225 to 450 W resulted in a rise in the zeta potential magnitude and a reduction in surface tension, respectively. FTIR and XRD analysis of the complexes revealed that an ultrasound intensity of 450 W enhanced the interaction between EPPs and OPI. The steady-state rheology data were fitted with a power law model. The viscoelastic characteristics of the interactions indicated that <i>G</i>′ exceeded <i>G</i>″, suggesting gel-like behavior of the samples. DSC and TGA analyses corroborated the FTIR results, demonstrating that the formation of noncovalent bonds (hydrogen and electrostatic) contributed to the increased thermal stability of the EPP/OPI complexes. The prebiotic potential of the EPP/OPI complex for <i>L. plantarum</i> and <i>L. rhamnosus</i> was found to be superior to that of inulin. The EPP/OPI complex derived from 450-W ultrasound exhibited enhanced rheological, thermal, and prebiotic properties, establishing 450 W as the optimal power for effective interaction between EPPs and OPI.</p></div>","PeriodicalId":562,"journal":{"name":"Food and Bioprocess Technology","volume":"19 10","pages":""},"PeriodicalIF":5.6,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149034718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}